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Citations for Promega :
2101 - 2150 of 3140 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The migrated cells in the lower chamber were stained with 5 μl of CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega # G3580) for 2.5 h at 37° C with 5% CO2 ...
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bioRxiv - Bioengineering 2023Quote: ... 1 well-1 with 100 μL fresh cell culture media) and mixed with 100 μL reaction solution of CellTiter-Glo 3D cell viability assay kit (G9681; Promega, WI). The 96-well plate was gently rocked for 5 min and stabilized at room temperature for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... cell viability was assessed using the MTS assay (CellTiter 96® AQueous One Solution Cell Proliferation, G3580, Promega, Madison, WI, USA). In brief ...
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bioRxiv - Developmental Biology 2023Quote: ... then incubated in freshly made stain solution (5mM potassium ferricyanide + 5mM potassium ferrocyanide + 1mg/mL X-gal (Promega cat no. V3941) in wash buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The gel slices were then dried again and treated with an ice-cold solution of 12.5 ng/μL trypsin (Promega, Madison, WI) in 100 mM ammonium bicarbonate ...
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bioRxiv - Biophysics 2023Quote: To investigate CBD influence on the cells viability and metabolic activity the cells were tested using CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega) with tetrazolium compound ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell viability was measured 72 hr after drug treatment using the CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... A 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS)-based viability assay (CellTiter 96® AQueous One Solution Cell Proliferation Assay, Promega) was performed as previously described (25).
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bioRxiv - Molecular Biology 2024Quote: ... The MTS assay was carried out at certain time points using The CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega #G3580) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The final reduced and alkylated flow through from the column was mixed with 1 mg/mL solution of trypsin enzyme (Promega, V511A) in ratio 1:10 (w/w ...
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bioRxiv - Microbiology 2024Quote: ... the morphology of the cells will be assessed and MTS tetrazolium compound (CellTiter 96 AQueous One Solution Assay, Promega, Madison, WI) was added ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were cultured for 72 hours and then measured for cell growth using an MTS assay (CellTiter 96® AQueous one solution cell proliferation assay, Promega). The IC50 values were generated from 3 independent experiments using Prism 8.0 (GraphPad ...
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bioRxiv - Microbiology 2024Quote: The 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H- tetrazolium (MTS) assay was performed using the CellTiter 96® AQueous One Solution Cell Proliferation Assay kit (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... NanoBRET plates were read after the addition of 50 μL of a stock solution (3X) containing NanoBRET NanoGlo substrate (Promega, #N2161), extracellular NanoLuc inhibitor (Promega ...
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bioRxiv - Cell Biology 2024Quote: ... cells were gently washed once with 1X DPBS and MTS reagent of 20 μL (Cell Titer-96 Aqueous One Solution, Promega, USA) in 80 μL of cell culture media for 3 h (37°C and 5% CO2) ...
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bioRxiv - Biochemistry 2024Quote: ... Cell proliferation was evaluated using a Promega CellTiter 96™ AQueous One Solution Cell Proliferation Assay (MTS) as per the manufacturer’s instructions (Promega G3580). The absorbance (490 nm ...
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bioRxiv - Immunology 2024Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
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bioRxiv - Genetics 2024Quote: ... cells were washed twice in PBS and incubated for 5 min at room temperature with 50 µL of a 50 µg/mL RNAse A solution (Promega A7973). Finally ...
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bioRxiv - Immunology 2024Quote: ... Gel pieces were then rehydrated with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... The pellet was resuspended in 100 µl 100 mM ammonium bicarbonate and 20 µl trypsin solution (#V5111, Promega, Madison, WI, USA) and then incubated at 37 ℃ for 16 hr ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized from 1 μg of each RNA sample using Oligo(dT)15 Primer (Promega) and M-MLV Reverse Transcriptase (RNase H Minus ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA samples were extracted using Eastep Super Total RNA Extraction Kit (LS1040, Promega, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were diluted to 1M urea using a stock of 50mM ammonium bicarbonate and trypsin (Promega) was added at a 1:50 enzyme-to-substrate ratio and incubated overnight at 37°C shaking at 600 rpm ...
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bioRxiv - Molecular Biology 2020Quote: ... The samples were dissolved in 0.1 M ammonium bicarbonate and digested with sequencing grade trypsin (Promega) at a protease/protein ratio of 1:50 ...
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bioRxiv - Molecular Biology 2020Quote: Samples were then incubated overnight at 37°C with 12.5 ng/μL trypsin (sequencing grade; Promega) in 25mM NH4HCO3 ...
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bioRxiv - Plant Biology 2020Quote: ... Pooled RNA samples derived from each conditions were subjected to DNaseI digest (RNase-free DNaseI, Promega) in the presence of RNase inhibitor (RNasin Plus ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were digested overnight at 37°C with 1 μg trypsin (sequencing grade; #V5111, Promega) per 100 μg of protein.
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bioRxiv - Microbiology 2020Quote: ... Extraction of DNA from each sample was done using the Wizard Genomic DNA Purification Kit (Promega) following the manufacturer’s specifications.
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bioRxiv - Cancer Biology 2020Quote: ... and RNA Tissue Miniprep System (Promega, UK, #Z6112 for tumor samples. GoScript™ (Promega, UK, #A5003) was used for the cDNA synthesis ...
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bioRxiv - Biophysics 2021Quote: ... Samples were observed either in an 8-well Lab Tek chamber slides (Promega, Madison, WI, USA) or a home-made Ludin chamber ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were mixed in a 1:1 (v/v) ratio with luciferase assay reagent (Promega) in a 96-well white opaque plate and left at room temperature for 2 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... Luciferase assays were performed on samples using the Dual-Luciferase Reporter Assay System (Promega, Madison, WI). Brains were placed in 200 μL of 1x passive lysis buffer and homogenized with a pestle ...
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bioRxiv - Microbiology 2020Quote: ... 150 μg of protein from each sample digested overnight/37 °C with 1.5 μg trypsin (Promega), followed by addition of further 1.5 μg of trypsin and digestion for an additional 4-hrs ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then subjected to immunoblotting and probed for anti-p75NTR (Promega, Cat: G323A, 1:300) and anti-GAPDH (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... 60 μL of each sample was mixed 1:1 with Steady-Glo Luciferase system (Promega #E2510) following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... Samples were diluted and the bioluminescent signal was quantified using Nano-glo Luciferase Assay (Promega, N1110). Readings were performed on a Berthold Sirius luminometer.
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bioRxiv - Cell Biology 2022Quote: ... the samples were mixed in a 1:1 (v/v) ratio with nucleotide detection reagent (Promega) in a 96-well white opaque plate and left at room temperature in the dark for another 60 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were mixed in a 1:1 (v/v) ratio with luciferase assay reagent (Promega) in a 96-well white opaque plate and left at room temperature for 2 minutes ...
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bioRxiv - Genomics 2021Quote: ... was extracted from tissue samples using a commercial kit (Wizard R Genomic DNA Purification Kit, Promega) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Reacted sequences were isolated by addition of one sample volume of Streptavidin MagneSphere paramagnetic beads (Promega) per sample ...
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bioRxiv - Microbiology 2021Quote: ... and 25 μg of each rNA sample was digested at 37°C overnight with trypsin (Promega). After 10 min at 95°C to denature the trypsin ...
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bioRxiv - Microbiology 2022Quote: ... Trypsin digestion was done using sequencing-grade modified Trypsin (0.4 μg/sample; Promega Corp., Madison, WI) overnight at 37°C ...
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bioRxiv - Plant Biology 2022Quote: ... Leaf samples were collected for RNA extraction using the SV Total RNA Isolation system (Promega, USA) and cDNA synthesis using the High-Capacity cDNA Reverse Transcription Kits (Applied biosystems ...
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bioRxiv - Plant Biology 2022Quote: ... equal RNA concentrations of each sample were used and incubated with reverse transcriptase MM-LV (Promega). A final PCR reaction using cDNA as template was performed as follows ...
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bioRxiv - Genomics 2024Quote: ... (i) quantification of DNA samples of multiple sexed green toads (QuantiFluor® dsDNA System kit, Promega), (ii ...
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bioRxiv - Cell Biology 2024Quote: ... Half of the sample was digested with 10 µg trypsin (Promega, 18 h at 37 °C). DOC was precipitated with 10 µL concentrated formic acid (conc ...
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bioRxiv - Molecular Biology 2024Quote: ... The samples underwent overnight on-bead digestion with 3.5 µl of trypsin (0.1 mg/mL, Promega) and were dissolved in 50 mM acetic acid in elution buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Protein samples (15 µg) were digested overnight at 37 °C with trypsin (Promega, Madison, WI-USA) in a protein:trypsin ratio of 50:1 (w/w) ...