Labshake search
Citations for Promega :
2101 - 2150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... and proteins were digested with LysC and trypsin (Promega) in two steps with a final enzyme-to-protein ratio of 1:25 ...
-
bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized using ImPromII reverse transcriptase system (Promega, Madison, WI) from 5 μl of stool or tissue RNA ...
-
bioRxiv - Microbiology 2024Quote: ... 100 µl of samples were mixed with 100 µl BacTiter-GloTM reagent (Promega) and incubated for 5 min in the dark ...
-
bioRxiv - Microbiology 2024Quote: ... Ligation was performed using T4 DNA ligase (Promega) with 3:1 insert:vector ratio in the buffer provided by the manufacturer ...
-
bioRxiv - Microbiology 2024Quote: ... Proteins were digested using filter-aided sample preparation with sequence-grade trypsin (Promega, # V5111) at 37°C overnight ...
-
bioRxiv - Immunology 2024Quote: ... supernatant was collected from the replicon-transfected cells and assayed for Renilla luciferase activity according to kit instructions (Promega, cat. E2810).
-
bioRxiv - Immunology 2024Quote: ... using Fugene HD transfection reagent (Promega). Human PBMC were pre-activated with 30 ng/ml anti-CD3 (OKT3 ...
-
bioRxiv - Immunology 2024Quote: ... During the experiment tumor growth was monitored weekly by BLI measurement after intraperitoneal (IP) Luciferin (Promega) injection.
-
bioRxiv - Microbiology 2024Quote: ... Prior to tryptic digestion of the protein overnight using mass-spec grade Trypsin (Promega), protein was reduced and alkylated using dithiothreitol and iodoacetamide ...
-
bioRxiv - Microbiology 2024Quote: ... following extraction RNA was treated with RNase -free DNase I (Promega) for 30 minutes ...
-
bioRxiv - Molecular Biology 2024Quote: ... with 100 bp ladder (Promega, USA) or with a TapeStation 4150 using D5000 screen tape (Agilent ...
-
bioRxiv - Microbiology 2024Quote: ... Media was removed from each well prior to addition of 100 μL Bright-Glo™ luciferase substrate (Promega) diluted 1:20 in 1x lysis buffer ...
-
bioRxiv - Microbiology 2024Quote: ... the MTS cell viability reagent (CellTiter 96 AQueous MTS Reagent from Promega) was added ...
-
bioRxiv - Microbiology 2024Quote: ... HEK293T cells (70% confluent) were transfected using FuGene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... The activity of in vitro synthesized luciferase was assessed using 5 μL of the substrate from the Steady-Glo Luciferase Assay System (Promega, US). The signal was detected on plate reader Victor X5 2030 (Perkin Elmer ...
-
bioRxiv - Microbiology 2024Quote: ... coli S30 Extract System for Linear Templates (Promega, USA). The reactions were carried out in 5 μL aliquots containing 2 μL of S30 Premix ...
-
bioRxiv - Microbiology 2024Quote: Lytic cell death was indirectly studied by measuring lactate dehydrogenase (LDH) release in cell culture medium using the CytoTox 96 nonradioactive cytotoxicity assay kit (G1780, Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2024Quote: ... HALO-tagged miniGs was labeled using HALO-JaneliaFluor595 (HALO-JF595) (Promega) by incubating cells in ∼2 μM of HALO-JF595 in DMEM supplemented with 10% FBS for 30 min at 37 °C ...
-
bioRxiv - Cell Biology 2024Quote: ... RNA samples were treated with RQ1 DNAse (Promega: M6101) and cDNA was synthesized using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2024Quote: ... DNA was isolated using the Maxwell® 16 DNA Purification Kit (Promega) and corresponding Maxwell instrument ...
-
bioRxiv - Microbiology 2024Quote: ... CellTiter-Glo 2.0 (Promega) was added to measure the fluorescence intensity ...
-
bioRxiv - Microbiology 2024Quote: ... Luminescence (relative luminesce units, RLU) and Absorbance (OD595) of sample triplicates supplemented with NanoGlo Live Cell Assay System (Promega) as recommended by the manufacturer ...
-
bioRxiv - Microbiology 2024Quote: ... rabbit anti-Halo (1:300, Promega), rabbit anti-H3K9me3 (1:300 Abcam ...
-
bioRxiv - Microbiology 2024Quote: ... 0.1 % ProteaseMax (Promega, USA)) ...
-
bioRxiv - Physiology 2024Quote: Total RNA was isolated from snap frozen LV wall or kidney after mechanical grinding (Precellys Evolution, Bertin Technologies, France) using the Maxwell RSC microRNA Tissue kit (Promega, USA). RNA was quantified via NanoDrop (Thermo Fisher Scientific ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Injection of 40 µl firefly luciferase substrate (Promega) and measurement of luminescence was performed with a plate reader (Viktor Nivo ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... 50 ng/well of pGloSensorTM-22F cAMP sensor plasmid (Promega), 50 ng/well of the 3x HA MC4R plasmid and 50 ng/well pcDNA3.1 or MRAP2-FLAG were added to 25 mL/well OptiMEM® media ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... The stimulation was stopped by discarding the media and cell lysis was induced by addition of 1x passive lysis buffer (PLB, Promega) and horizontal shaking for 15 min at room temperature.
-
bioRxiv - Plant Biology 2024Quote: ... The luciferase activity was detected using Dual-Luciferase® Reporter Assay System (Promega, E1910) by Infinite F200 Fluorescence Microplate Reader (Tecan).
-
bioRxiv - Plant Biology 2024Quote: ... proteins that underwent in vitro reactions were digested in 50 mM ammonium bicarbonate (NH4HCO3) with 0.2 µg trypsin (Promega) at 37°C overnight ...
-
bioRxiv - Plant Biology 2024Quote: Phosphorylation of HUB2 by SnRK2s was examined with ADP-Glo Protein Kinase Activity Assay Kit (Promega, V9101) with slight modifications (Kathania et al. ...
-
bioRxiv - Plant Biology 2024Quote: ... alkylated (55 mM Iodoacetamide; 1h @ 23°C) and digested in solution with 1:100 trypsin (V5113; Promega). Peptides were acidified to 0.5% (v/v ...
-
bioRxiv - Physiology 2024Quote: ... revelation was done by incubation with ECL Western Blotting Substrate (Promega, W1001). Signals were recorded with Amersham IQ800 imager (Cytiva) ...
-
bioRxiv - Physiology 2024Quote: CellTiter 96® Aqueous One solution Cell Proliferation Assay was used accordingly to manufacturer instruction (Promega). Briefly ...
-
bioRxiv - Physiology 2024Quote: ... the PCR products were ligated into the pGEM-T Easy Vector (Promega, Madison, WI), and the sequences were confirmed by Sanger sequencing ...
-
bioRxiv - Synthetic Biology 2024Quote: ... FuGENE HD transfection reagent (Promega E2312) was used as specified in the user manual to transfect 400 ng of the hypoxia and/or Wnt recording cassettes into the 24-well cultures ...
-
bioRxiv - Synthetic Biology 2024Quote: ... and the TdT variant of interest (750 ng) were transiently transfected using FuGENE HD Transfection Reagent (Promega E2312) according to manufacturer instructions ...
-
bioRxiv - Synthetic Biology 2024Quote: ... 20 ul of linear TXTL reaction were mixed with 50 ul of Nano-Glo luminescent substrate (Promega N1110) into a white-bottom 96-well plate ...
-
bioRxiv - Synthetic Biology 2024Quote: ... and 1 µg of an mCherry-expressing plasmid (included for a visual indicator of the transfection efficiency) were transfected into each flask with FuGENE HD transfection reagent (Promega E2312), according to the manufacturer’s instructions ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Females from each progeny were then pooled to perform genomic DNA extraction (Wizard® Genomic DNA Purification Kit, Promega) and subsequently do amplicon sequencing on the dsx target to check the frequency of the dsxFΔ11 allele in the offspring ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Larvae from progenies with low homing rates of dsxFGFP-null were pooled to perform genomic DNA extraction (Wizard® Genomic DNA Purification Kit, Promega) and subsequently do amplicon sequencing on the dsx target to check for the presence of mutations that could have prevented the homing ...
-
bioRxiv - Synthetic Biology 2024Quote: ... BacTiter-Glo™ Microbial Cell Viability Assay reagent was purchased from Promega (USA). We purchased B-PER™ Complete Bacterial Protein Extraction Reagent and Pierce™ 660 nm Protein Assay Reagent from Thermo Fisher Scientific (USA) ...
-
bioRxiv - Synthetic Biology 2024Quote: ... and the assay was carried out using the Nano-Glo® Luciferase assay system and associated protocol (Promega, Madison, USA). Samples were mixed with an equal volume of the Nano-Glo® assay reagent and 20 µL of this mix was subsequently pipetted into the wells of a Nunc™ 384-well microplate (Thermo Fisher Scientific ...
-
bioRxiv - Synthetic Biology 2024Quote: ... NanoLuc luciferase activity was with the kit (Promega) measured according to the manufacturer’s instructions.
-
bioRxiv - Physiology 2024Quote: ... MAO activity was measured in luminescence units (RLU) using extra protocol steps specific to MAO-B (Promega cat. V1401); PG has been shown to inhibit this isoform specifically.
-
bioRxiv - Plant Biology 2024Quote: ... followed by genomic DNA extraction with CTAB and RNase A treatment (Promega) (Chen and Ronald,1999) ...
-
bioRxiv - Plant Biology 2024Quote: ... 2 µL ROX (CRX reference dye, Promega, C5411) were added to 1 mL SybrGreen as a passive reference dye that allows fluorescent normalization for qPCR data ...
-
bioRxiv - Plant Biology 2024Quote: RNA was extracted using Maxwell plant RNA kit (Promega Corporation, Madison, WI, USA). After quality and quantity control ...
-
bioRxiv - Plant Biology 2024Quote: ... PCR amplification was performed in an 8 µl reaction mixture comprising GoTaq Green Master Mix (Promega), primers ...
-
bioRxiv - Physiology 2024Quote: ... The resulting cDNA product was inserted into the pCI-neo vector (Promega, Madison, WI, USA) between the EcoRI and XbaI sites ...