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Citations for Promega :
1901 - 1950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Each well was also transfected with a pGL3 vector (10 ng, Promega) as a transfection control ...
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bioRxiv - Biochemistry 2024Quote: ... The binding of the Nbs to the coated protein was detected via their EPEA-tag using a 1:4000 CaptureSelectTM Biotin anti-C-tag conjugate (Thermo Fischer Scientific) in combination with 1:1000 Streptavidin Alkaline Phosphatase (Promega). Colour was developed by adding 100 µL of 3 mg/mL disodium 4-nitrophenyl phosphate solution (DNPP ...
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bioRxiv - Biochemistry 2024Quote: ... Toxin sensitivity was measured with CellTiter-Glo reagent (Promega) according to manufacturer protocol ...
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bioRxiv - Biochemistry 2024Quote: ... after which cells were read for luminescence signal using the NanoGlo® Luciferase Assay kit (Promega), on a SpectraMax M5e plate reader (Molecular Devices) ...
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bioRxiv - Neuroscience 2024Quote: ... The Renilla luciferase activity associated with WT or uORF-mutated 5’UTRs was measured according to the manual of the Dual-Luciferase Reporter Assay System (Promega) 32 h after transfection and was normalized to the activity of firefly luciferase.
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bioRxiv - Animal Behavior and Cognition 2024Quote: We extracted total RNA by combining a TRIZOL-chloroform approach with a spin column kit (SV Total RNA Isolation System, Promega (Peuß et al., 2016)) ...
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bioRxiv - Neuroscience 2024Quote: ... The WT and mutated 5’UTR sequences were ligated into a linearized reporter plasmid (psiCHECK-2 vector, Promega). The entire sequences of all the plasmids were validated by Sanger sequencing ...
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bioRxiv - Biochemistry 2024Quote: ... The wild-type and mutated GCE variants were expressed in vitro using the TnT Quick T7 Coupled translation rabbit reticulocyte lysate system (Promega, Madison, WI, USA). The reaction products were examined in the dextran-coated charcoal binding assay4,9 with own synthesized [3H]JH III (25.7 Ci/mmol).62 The reticulocyte lysate without DNA input was the background control.
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bioRxiv - Biochemistry 2024Quote: ... intracellular ATP levels were quantified using the CellTiter-Glo 2.0 luminescence assay (Promega, Madison, WI, USA). Luciferase activity was recorded on the Envision plate reader (PerkinElmer ...
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bioRxiv - Biochemistry 2024Quote: ... were prepared by substituting the firefly luciferase sequence with that encoding the NanoLuc® luciferase (Promega, Madison, WI, USA). The NanoLuc® sequence was excised from a previously described vector pNL-9xUAS [nLucP Hygro]62 using HindIII and BamHI ...
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bioRxiv - Biochemistry 2024Quote: ... Bright-Glo™ Luciferase Assay System (Promega, Madison, WI, USA) was used for the firefly-based reporters and Nano-Glo® Luciferase Assay System (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... Kc167 cells were seeded in 6-well plates and transfected (3 µg DNA per well) with the JHRE reporter or control (EGFP-pIEx-4) plasmids using the standard FuGENE-HD transfection protocol (Promega, Madison, WI, USA). About 48 h post-transfection ...
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bioRxiv - Biochemistry 2024Quote: ... and mixed with the nanoluciferase substrate Nano-Glo (Promega cat# N1120, final dilution 1:200) for 2 min before measuring luminescence in a POLARstar OMEGA plate reader (BMG Labtech ...
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bioRxiv - Biochemistry 2024Quote: ... The plasmid encoding Glosensor 22F was acquired from Promega (cat# E2301). The plasmid encoding cytosolic KB1691-Nluc (pcDNA3.1-cyto-KB1691-Nluc ...
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bioRxiv - Biochemistry 2024Quote: ... 7C (mouse, Promega cat# N700A; 1:500-1:1000); Nluc in Fig ...
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bioRxiv - Biochemistry 2024Quote: ATP hydrolysis rates were measured using the ADP-Glo Kinase assay (Promega). Reactions were performed with 500 μM ultra-pure ATP in a reaction buffer containing 20 mM Tris pH 7.5 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell proliferation in vitro was assessed using CellTiter (Promega) per the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... and reverse-transcribed with AMV-RT (Promega) and random 9-mer primers ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were labelled at least 30 minutes prior to imaging with Halo ligand Janelia Fluor 646 (Promega) at a 1:1,000 dilution of 20 μM stock in DMSO and 10 uM Hoesct dye ...
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bioRxiv - Cancer Biology 2024Quote: ... and MTT-based cell viability reagent (CellTiter96, Promega, Madison, WI) was added at 48 h and allowed to incubate for ∼2 h before reading ...
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bioRxiv - Cancer Biology 2024Quote: ... the fusion proteins were expressed using the cell-free transcription and translation system (Cat. # L4140, Promega) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... human Female genomic DNA (Promega #G1521), as a reference ...
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bioRxiv - Cancer Biology 2024Quote: ... CellTiter-Glo reagent (Promega) was used to assess cell viability at multiple time points after transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... cell viability was analyzed using the CellTiter-Glo Luminescent Cell Viability Assay (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... CellTiter-Glo 2.0 (#G9243, Promega, Madison, Wisconsin, USA) was added to each well in a 1:1 v/v ratio ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 ng of Renilla luciferase expressing pRL SV40 plasmid (E2231, Promega), as internal control ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were harvested at 48 h post-transfection and activity of Firefly and Renilla luciferases were sequentially determined using the Dual-luciferase Reporter Assay System (E1980, Promega) and a Beckman Coulter microtiter plate reader (Beckman Coulter ...
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bioRxiv - Cancer Biology 2024Quote: ... Digestion was carried out using sequence-grade modified trypsin (Promega, Madison, WI) at an enzyme-to-protein ratio of 1:50 (w/w ...
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bioRxiv - Cancer Biology 2024Quote: ... after DNase I treatment with RQ1 RNase-Free DNase (Promega - M6101), reverse transcription was done using OneScript Plus cDNA Synthesis Kit (abm-G236) ...
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bioRxiv - Cancer Biology 2024Quote: ... with 200U/mL RNAsin (Promega) (3x pellet volume ...
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bioRxiv - Cancer Biology 2024Quote: ... with 200U/mL RNAsin (Promega) (3x pellet volume ...
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bioRxiv - Cancer Biology 2024Quote: ... Assay run in technical triplicate following manufacturer instructions (G8080, Promega).
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bioRxiv - Cancer Biology 2024Quote: ... 0.5 µg of trypsin/Lys-C mixture (V5073, Promega) added to each on-bead sample and set to rotate head-over-head overnight at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... Viability of tumor organoids was assayed using RealTime-Glo™ MT Cell Viability Assay by Promega with heme treated organoids compared to their baseline activity.
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bioRxiv - Cancer Biology 2024Quote: ... Instructions from Glutamine/Glutamate-Glo Kit (J8021, Promega) were followed past this point.
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bioRxiv - Cancer Biology 2024Quote: ... The cell viability was measured by adding MTS reagent (Cat. No. G-111, Promega, Madison, WI) according to the manufacturer’s protocol and as described previously.(26 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was tested after 96hrs of transfection using MTS kit (Promega) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... Luciferase intensity was recorded 48 h after transfection using the dual-luciferase reporter assay system (Promega, Madison, WI).
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bioRxiv - Cancer Biology 2024Quote: ... HeLa cells were routinely tested negative for Mycoplasma (Promega PK-CA20-700-20).
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was measured by CelltiterGlo assay (G7571, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were subjected to cell viability assay using CellTiter-Glo assay (# G7572, Promega, USA) in accordance to the manufacture protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were subjected to cell viability assay using CellTiter-Glo assay (# G7572, Promega, USA) in accordance to the manufacture protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... containing 50mM DTT (Promega, #V3151), vortexed and heated up to 98°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Horseradish peroxidase-conjugated secondary antibodies (Promega) and diaminobenzidine (Cell Signaling ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was isolated with the Maxwell 48 RSC simply RNA Tissue Kit (Promega) on a Maxwell RSC Instrument (Promega) ...
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bioRxiv - Cancer Biology 2024Quote: ... Moloney Murine Leukemia Virus Reverse Transcriptase RNase H-Point Mutant (Promega) was used for cDNA synthesis ...
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bioRxiv - Cancer Biology 2024Quote: ... and GoTaq qPCR Mastermix (Promega) for quantitative real-time PCR on a CFX384 instrument (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: ... on a Maxwell RSC Instrument (Promega). Moloney Murine Leukemia Virus Reverse Transcriptase RNase H-Point Mutant (Promega ...
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bioRxiv - Cancer Biology 2024Quote: Organoid growth was determined by using the CellTiter-Glo Luminescent Cell Viability Assay Kit (Promega, G7573) according to the manufacturers’ protocol ...
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bioRxiv - Cancer Biology 2024Quote: Viability assays were performed using the CellTiter-Glo Luminescent Cell Viability Assay (Promega #G7572) per manufacturer’s protocol ...