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Citations for Promega :
2051 - 2100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... and 50 U/ mL DNAse (# M6101, Promega) at 37°C for 1 hr rotating incubator ...
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bioRxiv - Cell Biology 2024Quote: mRNA was synthesized in vitro with linearized template plasmids using the RiboMax Large Scale RNA Production System-T7 (Promega) supplemented with the Ribo m7G Cap Analog (Promega) ...
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bioRxiv - Cell Biology 2024Quote: RAW 264.7 cells were transfected using FuGene HD (Cat# E2311, Promega) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and then cells were homogenized according to the manufactureŕs instructions (Dual-Luciferase resporter assay system, Promega, Madison, WI, USA)
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bioRxiv - Cell Biology 2024Quote: ... samples were treated with RNase-Free DNase Kit (#M6101; Promega), quantified ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were digested with 1µg trypsin (Trypsin Gold, Promega Corporation) overnight at 37°C in a shaker at 1250 rpm ...
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bioRxiv - Cell Biology 2024Quote: 1μg of each plasmid or pcDNA3.1 empty vector was reverse transfected into 1x106 HEK293T cells using FUGENE HD (Promega) as per manufactures instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Trypsin (Promega) was added at 1:80 enzyme/protein ratio and digestion performed overnight at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were harvested after 24 h and luciferase activity was measured using the Luciferase Reporter Assay System (Promega, Cat. #E1500) on Victor Nivo luminometer (PerkinElmer) ...
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bioRxiv - Cell Biology 2024Quote: ... Each sample was digested on beads in 100 µl of 50 mM ammonium bicarbonate with 1 µg trypsin (Promega) in spin cap tubes sitting in fresh mass-spectrometry grade Eppendorf tubes ...
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bioRxiv - Cell Biology 2024Quote: ... The DNA fragments were PCR-amplified with primer pairs containing a XhoI and BamHI site from human genomic DNA (Promega) and subcloned into the pCLL-NoPromoter-FLuc-CMV-RLuc-dsRed2 vector ...
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bioRxiv - Cell Biology 2024Quote: ... cell lysis was achieved by adding 50 μL of Steady-Glo Luciferase Assay System buffer (Promega, E2510) to the wells ...
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bioRxiv - Cell Biology 2024Quote: ... Transfection of HeLa cells with VPS35 plasmids was achieved using FuGene HD (Promega, #E2311) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected with TPMT-FLAG or TPMT A80P-FLAG with FuGENE 6 (Promega). 24 h post transfection ...
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bioRxiv - Cell Biology 2024Quote: ... Purification and assembly of DNA fragments were performed using a Wizard SV Gel and PCR Clean-up System (Promega) and a NEBuilder HiFi DNA assembly cloning kit (New England Biolabs) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell proliferation was evaluated using CellTiter 96® Aqueous MTS assay (Promega; Madison, WI) as previously described [17] ...
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bioRxiv - Cancer Biology 2024Quote: We developed a high-throughput cytotoxicity plate based-assay using the Cell Titer-Glo® 2.0 Luminescent Cell Viability Assay (Promega, G9242) to screen CBCS Oncoset collection of 172 compounds with known targets (listed in table S1 ...
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bioRxiv - Cancer Biology 2024Quote: ... Denatured proteins were enzymatically digested for 14 h at 37 °C with 1 µg of sequencing-grade Trypsin (V511A, Promega). After speed-vaccum drying ...
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bioRxiv - Cancer Biology 2024Quote: ... 3 μl MgCl 25 mM (Promega A3511), 0.2 mM dNTPs ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was measured using CellTiter-Glo (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... we added 10μL Cell Titer Glo reagent diluted 1:2 (Promega, Inc.) to each well and mixed ...
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bioRxiv - Cancer Biology 2024Quote: ... the kinase reaction mixtures were collected after incubation and added into equal volume Kinase-Glo® regent (V6711, Promega) for 10 minutes at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Reactions were treated with RQ1 DNAse (Promega M6101) for 20 min at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... gDNA was extracted from a positive clone using the Wizard Genomic DNA Purification Kit (Promega) and subjected to whole genome sequencing (Novogene ...
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bioRxiv - Neuroscience 2024Quote: ... the medium was removed and substituted with OptiMEM media supplemented with 4 µM coelenterazine-H (Promega; Cat. No. S2001), and incubated for a period of 2 to 3 hours ...
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bioRxiv - Microbiology 2024Quote: ... DNA was purified using the Wizard Genomic DNA Purification kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was then reverse transcribed to cDNA using the AMV reverse transcriptase (Promega, M510F). qPCR experiments were performed to assess the levels of cDNA using primers referenced in Extended Data Table 1 ...
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bioRxiv - Molecular Biology 2024Quote: Cytotoxicity was measured by Lactate Dehydrogenase (LDH) release using the CytoTox 96 Non-Radioactive Cytotoxicity Assay kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (E1910, Promega corporation, Madison, WI), and subsequently used to infect suspension cultures of Sf9 cells (2 × 106 cells/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... Viral replication was measured from supernatants collected 24 hrs post infection and viability determined at 48 hrs by 3D CellTiter-Glo assay following the manufacturer’s instructions (Promega).
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bioRxiv - Bioengineering 2024Quote: ... RNA was reverse transcribed to cDNA using random primers and GoScript Reverse Transcriptase kit (Promega). RT-qPCR was performed using Firepol qPCR Master Mix (Solis BioDyne ...
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bioRxiv - Biophysics 2024Quote: ... Promega Chemiluminescent Substrate (Promega) was used according to the manufacturer’s instructions to visualize TTR content.
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bioRxiv - Biochemistry 2024Quote: lrl 1μg of N-terminally FLAG-tagged receptor and 1μg of F22 (Promega, Cat. no: E2301) (for GloSensor assay)
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bioRxiv - Genetics 2024Quote: ... total RNA was isolated from the transfected cells using Trizol reagent (Thermo Fisher Scientific, Waltham, MA, USA and reverse transcription was performed using the GoScript™ Reverse Transcription System (Promega, Madison, MI, USA). PCR amplification was performed using the pCAS2-RT-F and pCAS2-RT-R primers ...
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bioRxiv - Genetics 2024Quote: ... cell viability was determined using CellTiter-Glo Luminescence Cell viability Assay kit (Promega) by measuring luminescence (ATP level ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293T cells were transfected with 3.5μg of N-terminally FLAG-tagged CXCR2/CXCR3/CXCR7 and 3.5μg of F22 (Promega, Cat. no: E2301). 14-16h post-transfection ...
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bioRxiv - Bioengineering 2024Quote: ... Quenchbody PCR products were purified using Wizard Clean up kits (Promega; as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... GRK371 and β-arrestin272 recruitment to chemokine receptors (CXCR1, CXCR2, CXCR3-A, CXCR4, CXCR5, CXCR6 and ACKR3) was monitored using a nanoluciferase complementation-based assay (NanoBiT, Promega)73,74 ...
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bioRxiv - Bioengineering 2024Quote: ... A pHR expression plasmid and lentiviral packaging plasmids were co-transfected using the Fugene HD transfection reagent (Promega). 52 hours after transfection ...
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FACS-Sortable Picoreactors for Ultra High-throughput Screening of Catalysts in Biphasic EnvironmentsbioRxiv - Bioengineering 2024Quote: ... emulsions of octanol + 5% (w/v) Span 80 + 40 µM Nile Red in an aqueous phase of nuclease free water (Promega) + 200 ng/µL miniprepped plasmid + 5% (w/v ...
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bioRxiv - Bioengineering 2024Quote: ... a released luciferase assay was performed using the luciferase assay system (Promega). Medium was removed and cells were washed with 100 uL of PBS ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293T cells were transfected with 3.5μg of N-terminally FLAG-tagged receptor and 3.5μg of an SRE-based luciferase reporter plasmid pGL4.33 (Promega, Cat. no: E1340). 14-16h after transfection ...
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bioRxiv - Biochemistry 2024Quote: ... followed by the addition of ADP GloTM Kinase detection reagent (Promega #V6930) to convert ADP to ATP and introduce luciferase to detect ATP ...
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bioRxiv - Bioengineering 2024Quote: ... cells were lysed with Reporter Lysis Buffer (Promega, Madison, WI, USA, cat.: E3971) and freeze-thawed at −80°C ...
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bioRxiv - Biochemistry 2024Quote: ... Then membranes were incubated with secondary anti-rabbit IgG horse radish peroxidase (Promega, 1:5000) for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: The ADP-Glo™ Kinase Assay kit (V9104) was purchased from Promega Corporation (Madison ...
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bioRxiv - Biochemistry 2024Quote: ... Trypsin Golden (Promega, USA), dithiothreitol (DTT ...
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bioRxiv - Biochemistry 2024Quote: ... 100-fold diluted NanoBRET™ Nano-Glo® Substrate (Promega) was added and donor emission at 450 nm and acceptor emission at 618 nm were read within 10 min of substrate addition using a ClarioStar plate reader (Mandel Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... +/- 1000-fold diluted HaloTag® NanoBRET™ 618 Ligand (Promega) and incubated with indicated compounds concentrations or DMSO control for 4 h ...
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bioRxiv - Biochemistry 2024Quote: ... Trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, WI, USA) was used to digest proteins at 37 °C for 16 h.