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Citations for Promega :
2151 - 2200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... The plasmid DNA was extracted using the PureYield™ Plasmid Midiprep system (Promega, USA), followed by vacuum concentration in a SpeedVac Concentrator (Thermofisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... or Chymotrypsin (V1062, Promega) was performed at an enzyme-to-substrate ratio of 1:25 for 4 h at 37°C (Trypsin ...
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bioRxiv - Plant Biology 2024Quote: ... 150 mM NaCl pH 7.5 with 0.2% SDS and in solution digest using either Trypsin (V5280, Promega, Madison, USA), GluC (90054 ...
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bioRxiv - Plant Biology 2024Quote: ... proteins were digested overnight at 37°C with 2 µg trypsin (Promega). Phase transfer surfactants (such as SLS ...
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bioRxiv - Plant Biology 2024Quote: ... Then 0.2 µg Trypsin (Promega) was added to the beads ...
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bioRxiv - Plant Biology 2024Quote: ... and FUL1 were cloned into a plant expression vector as effectors.Using transient transformation of tobacco leaves and the Dual-Luciferase reporter assay system (Promega, Madison, WI, USA), we tested the ratio of LUC to REN to determine the activation of the SlVI promoter by these transcription factors.
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bioRxiv - Synthetic Biology 2024Quote: ... The Promega PureYield Plasmid Midiprep System (Promega) with vacuum manifold and GeneJET Plasmid Midiprep Kit (ThermoFisher ...
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bioRxiv - Synthetic Biology 2024Quote: ... FuGENE HD Transfection Reagent with a 20-minute complexing time was used to introduce the transgenes into the cells (Promega E2312). For stable cell lines ...
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bioRxiv - Synthetic Biology 2024Quote: Genomic DNA was isolated from plant cells using the Promega Wizard® SV 96 Genomic DNA Purification System (Promega Corporation, Madison, WI, USA) following the manufacturer’s protocol using the Tecan Fluent (Supplementary Figure S2E).
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bioRxiv - Synthetic Biology 2024Quote: ... 0.06pmol of donor backbone and 0.04pmol of All-in-One backbone were co-transfected in HEK293T using FuGENEHD (FuGENE® HD Transfection Reagent, Promega) as transfection reagent at a ratio of 3:1 FuGENEHD (μL) ...
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bioRxiv - Synthetic Biology 2024Quote: ... DNA yields were quantified (QuantiFluor® kit, Promega), and DNA concentrations were normalized to 2 ng/100 bp of amplicon length and submitted for Sanger sequencing with the appropriate forward PCR primer ...
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bioRxiv - Systems Biology 2024Quote: ... 110,6ul of Fugene HD (Promega) was added to the mix.
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bioRxiv - Developmental Biology 2024Quote: ... and 1:10000 of the HRP-conjugated Anti-Rabbit IgG (Promega W401B) and Anti-Mouse IgG (Promega W402B) ...
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bioRxiv - Developmental Biology 2024Quote: Animals were incubated in Z-VAD-FMK (Promega, Cat# G7232) at a final concentration of 10 µM for the hydrozoan species and 100 µM for the scyphozoan species in their respective fresh filtered (using 0.22 µm Millex® PVDF syringe filter ...
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bioRxiv - Genomics 2024Quote: ... The extraction was completed according to the Maxwell® RSC 48 Instrument (Promega, Madison, WI) protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and used the GoScript Reverse Transcription System kit to generate cDNA from extracted RNA (Catalog #A5000, Promega Corporation, Madison, WI). We designed primers using the tool primer-Blast134 and the predicted cDNA sequence of kitlga (Fig ...
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bioRxiv - Immunology 2024Quote: ... Anti-Mouse IgG HRP (1:20,000 Promega W402B), Anti-Rabbit IgG HRP (1:20,000 Promega W401B) ...
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bioRxiv - Immunology 2024Quote: ... and transfected the next day using FuGene HD transfection reagent (Promega) at a 1:3 DNA:transfection reagent ratio ...
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bioRxiv - Genetics 2024Quote: ... The assay was conducted on a Glomax Luminometer (Promega), following the guidelines provided by the manufacturer.
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bioRxiv - Genetics 2024Quote: ... followed by DNase (Promega) treatment ...
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bioRxiv - Microbiology 2024Quote: ... for intra-subcellular (i.e., vacuolar) conversion of glutamine (Gln) to glutamate (Glu) assay using Glutamine/Glutamate-Glo Assay kit (Promega, Madison, WI; Cat. # J8021) following kit’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... and lysed 72 hours later using the Bright-Glo Luciferase reagent (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Antisense DIG-tagged probes were synthesized by in vitro transcription using T3 polymerase (Promega) and purified using a Sephadex G-50 column ...
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bioRxiv - Genomics 2024Quote: ... Trypsin (Promega, Leiden, The Netherlands) was added to gel plugs for digestion ...
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bioRxiv - Immunology 2024Quote: ... A buffer containing 10 mM Tris HCL (H5121; Promega), 0.1 mM EDTA (AM9260G ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Luciferase activities were measured at 36-48 hours post-transfection using Dual-Luciferase Reporter Assay System (Promega) using Tecan Spark Multimode Microplate Reader (Eastwin International Trading Ltd).
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bioRxiv - Neuroscience 2024Quote: ... Trypsin and LysC (Promega) were added at a 1:100 (enzyme:protein w:w ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was measured by CellTiter-Glo bioluminescence (Promega, catalog no. G7572), and analyzed using an IVIS imager.
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bioRxiv - Cancer Biology 2024Quote: ... bulk ATP quantitation was performed 72 h after treatment using CellTiter-Glo® 2.0 (CTG; #G9243, Promega, Germany), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... dye was mixed with 10 nM JF646 dye (GA1120; Promega). To label the Halo-CAAX cells ...
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bioRxiv - Cell Biology 2024Quote: ... 24h post-transfection luciferase activity was measured using a Dual luciferase assay kit (#E1910) from Promega according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Apoptosis was determined as Caspase-3/7 activity immediately after 6-day culture using Caspase-Glo 3/7 Assay (Promega, USA). Luminescence was recorded with the Infinite M200 PRO microplate reader (Tecan ...
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bioRxiv - Cell Biology 2024Quote: ... Media samples were used immediately in the Cholesterol/Cholesterol Ester-Glo (Promega, J3191). Assays were performed according to manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: Correlative light and electron microscopy was conducted as previously described.61 PDZD8-Halotag knocked-in HeLa cells were overexpressed with plasmids encoding Venus-FKBP8N403K or YFP-ActA and then treated with 200 nM Janelia Fluor 549 dye (Promega) for 20 hours at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were processed and luciferase activity was measured using the Dual-Luciferase Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: The intracellular Ca2+ - release induced by hemin was measured by Fluo4 fluorescence intensity with a plate reader (GloMax®-Multi Detection System, Promega GmbH, Walldorf, Germany). Isolated platelets (6 x 107 per sample ...
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bioRxiv - Bioengineering 2024Quote: ... samples were incubated with sequencing grade trypsin (0.01 mg/mL; Promega Corporation) overnight at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... and incubated with the following primary antibodies diluted in TBS-T overnight at 4°C: mouse anti-HaloTag (1:5,000; Promega, G9211), mouse anti-Flag M2 (1:2000 ...
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bioRxiv - Bioengineering 2024Quote: ... a rATP standard (Promega, P1132) was prepared by diluting the 10mM stock 1:1000 in culture medium ...
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bioRxiv - Biochemistry 2024Quote: ... 20 μl Dual-Glo Stop reagent (Promega) was added into the above mixture to measure the Rluc luminescence ...
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bioRxiv - Biochemistry 2024Quote: ... using FuGENE6 (Promega), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... as primary antibody and an anti-rabbit IgG HRP-conjugated antibody (Promega) as secondary antibody ...
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bioRxiv - Immunology 2024Quote: Pyroptotic cell death was measured by assessing LDH-release in the cell culture supernatant of human and murine macrophages using a CytoTox 96 Non-radioactive Cytotoxic Assay (Promega) following the manufacturer’s recommended procedures.
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bioRxiv - Cancer Biology 2024Quote: ... PCR was performed using the Go TaqTM Master Mix (PRM7123, Promega).
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bioRxiv - Cell Biology 2024Quote: ... RNA concentration was measured with Quantus Fluorometer (Promega E6150). RNA quality was assessed by RNA ScreenTape analysis at Genomics Core Facility at the University of North Texas Health Science Center ...
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bioRxiv - Bioengineering 2024Quote: ... and Glutamate-GloTM (Promega, #J7021) assay kits respectively as per manufacturer protocols.
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was then determined using the CellTiter-Glo assay (Promega), with luminescence measured by a PerkinElmer Envision plate reader ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were digested overnight at 25°C with modified trypsin (Promega) using an enzyme-to-substrate ratio of 1:50 in 100 mM ammonium bicarbonate ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% of an equal molar mix of unmethylated lambda DNA (Promega, D1521) and fully methylated T7 was spiked into genomic DNA ...
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bioRxiv - Cancer Biology 2024Quote: ... The drug and media were refreshed on day 3 and cell viability was assessed in the cell lines as compared with the vehicle condition (0.1% DMSO) at 7 days post treatment using MTS reagent (Promega, Madison, WI, USA). IC50 values were determined in the cell lines by dose-response curves calculated using the drc (v3.0.1 ...