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Citations for Promega :
1151 - 1200 of 1604 citations for Phthalic Acid Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: A total of 100 μg of protein was digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, WI, USA) using filter-aided sample preparation (FASP ...
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bioRxiv - Immunology 2022Quote: ... plates were equilibrated at room temperature for at least 30 minutes before 100 µL of 1:1 diluted PBS and CellTiter Glo reagent (Promega) was added to 100 µL of media ...
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bioRxiv - Microbiology 2022Quote: ... were anesthetized with isoflurane and imaged immediately under an in vivo imaging system (IVIS, AMI HTX) after retro-orbitally injected with 100 µl of Nano-Glo luciferase substrate (Promega). The bioluminescence data acquisition and analysis were performed using the Aura program (Spectral Imaging Systems) ...
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bioRxiv - Physiology 2023Quote: ... 300 μl aliquots from each fraction after polysomal ultracentrifugation in sucrose density gradients were spiked with 100 pg of Luciferase mRNA (internal control, Promega). Then ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins bound to magnetic beads were washed twice with 100 μL of 25 mM NH4HCO3 and on-beads digested with 200 ng of trypsine/LysC (Promega) for 1 hour in 100 μL of 25 mM NH4HCO3 at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... expressing PALLD and pNLF1-N [CMV Hygro] or pNLF1-C [CMV Hygro] vector (Promaga) expressing ANKRD1 were treated with 100 nM HaloTag NanoBRET 618 Ligand (Promega), whereafter signals were detected six hours later using a Synergy 4 instrument (BioTek) ...
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bioRxiv - Plant Biology 2022Quote: ... Protein precipitates were resolubilized in 5% trifluoroethanol of aqueous 100 mM TEAB and digested overnight (18 hours) with a Trypsin+LysC mixture (Promega) at a protein to protease ratio of 25:1 ...
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bioRxiv - Immunology 2022Quote: ... were sorted by FACS in 96-well V-bottom plate wells containing 2 μl of lysis buffer (Ultra-pure Water, 10% Triton X-100, RNasinPlus 40U/ul; Promega). After evaporation of the lysis buffer at 95°C for 3 min ...
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bioRxiv - Biochemistry 2022Quote: ... Each 70 μl reaction is separated into three individual 20 μl reactions and 100 μl of Renilla Luciferase assay substrate (Promega) is added to each reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... were mixed with 75ul of 0.1% Triton X-100 in PBS and 25 μl of substrate (Bright-Glow™ Luciferase Assay System, Promega), and 50ul aliquots of the mix were distributed in triplicate wells of a 96-wells white plate and analyzed in a microplate luminometer.
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bioRxiv - Microbiology 2023Quote: ... 100 μL of these purified Se cells or 100 μL of the corresponding Se-Ai cocultures were mixed with 100 μL Nano-Glo Luciferase assay reagent (50:1 mixture of substrate:buffer, Promega N1110) in a 96-well plate ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were subjected to in gel tryptic digest overnight at 37 °C with approximately 2 µg trypsin per 100 µl gel volume (Trypsin Gold, mass spectrometry grade, Promega). Peptides were eluted twice with 100 mM NH4HCO3 followed by an additional extraction with 50 mM NH4HCO3 in 50% acetonitrile ...
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bioRxiv - Cancer Biology 2023Quote: ... with 250 cells plated per well in 96-well flat-bottom plates in 100 μl medium and an equal volume of the CellTiter-Glo Luminescent Assay Reagent (#G7571; Promega) added at the indicated time-points ...
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bioRxiv - Microbiology 2023Quote: ... For transient expression experiments each well was transfected with 100 ng of DNA using 1:3 ratio of FUGENE HD (#E2311, Promega). Twenty-four hours after transfection or doxycycline stimulation ...
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bioRxiv - Biochemistry 2023Quote: ... Samples containing 20-30 pmol rhodopsin were mixed with 100-250 fmol BSA and cleaved with 1 μg trypsin/LysC mix (Promega) using the SP3 beads protocol described in [29].
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed after exactly 24 hours of stimulation in 1X Passive Lysis Buffer (100 μl per well (Promega, #E1941)) ...
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bioRxiv - Biochemistry 2023Quote: ... The gel pieces were dehydrated using 100% ACN before 25 µl of 12 ng/µl sequence grade modified trypsin porcine (Promega) in 25 mM ABC was added ...
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bioRxiv - Cell Biology 2023Quote: ... Isolated proteins were digested with 1:50 w/w LysC (Wako Chemicals, cleaves at the carboxylic side of lysine residue) and 1:100 w/w trypsin (Promega, Sequencing-grade ...
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bioRxiv - Cell Biology 2023Quote: ... diluted 1:100 in storage buffer and analyzed as indicated in the manufacturer’s protocol (LDH-Glo Cytotoxicity Assay, Promega, #J2381).
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bioRxiv - Microbiology 2023Quote: ... After removal of supernatant cells were resuspended in 100 ul of freshly prepared 1X Renilla Luciferase Assay Lysis buffer (Promega). Samples were analyzed with the Renilla Luciferase Assay System (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... The library preparation involved using 100 ng of genomic DNA spiked with 1% (w/w) of unmethylated lambda DNA from Promega. Subsequently ...
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bioRxiv - Biochemistry 2022Quote: ... The transfected neurons were stimulated with 200μM glycine for 3 min and then washed for 30 minutes before lysis in 100 μl of buffer for dual luciferase assay (Promega). 20 μl of the lysates were used for the quantification of Firefly and Renilla Luciferase activity using a Luminometer (GloMax ...
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bioRxiv - Cell Biology 2022Quote: ... on-bead digestion was performed by addition of 100 μL trypsinization buffer (50 mM ammonium bicarbonate, Sigma and 300 ng trypsin per column, Trypsin-gold V5280 Promega) for 1 h at 23°C ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... Protein precipitates were resolubilized in 5% trifluoroethanol of aqueous 100 mM TEAB and digested for 18 h with Trypsin+LysC (Promega) at a ratio of 25:1 protein:protease ...
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bioRxiv - Microbiology 2023Quote: 1 ml of recombinant GST or GST-YgfB protein at a concentration of 10 μM was incubated with 100 μl 50 % MagneGST (Promega) bead-slurry equilibrated with pulldown-buffer for 45 min at 4°C and washed two times with 500 μl pulldown-buffer ...
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bioRxiv - Molecular Biology 2023Quote: The sequences of wild-type (WT) F8 exons and 100-250 nucleotides flanking each exon were amplified from human genomic DNA (Promega) using WT PCR primers shown in Supplemental Table 1 ...
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bioRxiv - Immunology 2023Quote: ... prepared by adding NanoDLR™ Stop & Glo® Substrate 1:100 into NanoDLR™ Stop & Glo® Buffer (Promega, USA), were added to the samples ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then washed with 100 μL PBS and lysed by addition of 20 μL 1 x Passive Lysis Buffer (Promega) to each well and nutated for 15 minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... The following day 100 μl of each sample was transferred to a white 96-well plate and 100 μl of the the mix of Bright-GloTM Luciferase Assay (Promega) was added to each well ...
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bioRxiv - Biochemistry 2023Quote: ... beads were resuspended in 100 μL of 50 mM TEAB and enzymatically digested overnight using sequencing-grade trypsin (Promega, V5111). Digested peptides were eluted through centrifugation and labeled using commercially available TMTsixplex tags (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... Clones expressing the desired fusion gene were determined first by staining with 100 nM JF549-HTL (Cat. No. GA1110, Promega) and 50 nM Hoechst 33342 and identifying clones with the expected distribution of JF549 signal ...
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bioRxiv - Immunology 2023Quote: ... cDNA was generated from 20-100 ng of RNA using the GoTaq 2-step RT-qPCR System (Promega, cat# A6110). qPCR was performed with SYBR Green on a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: For NanoBIT experiments 12,000 HeLa cells were seeded in 96-well black plates and each well was transfected with 100 ng of total DNA using 1:3 ratio of FUGENE HD (#E2311, Promega). Different amounts of plasmid DNA were used to achieve comparable expression of LgBiT/FLAG-tagged and SmBiT/V5-tagged proteins ...
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bioRxiv - Microbiology 2023Quote: ... and each cell pellet was fully resuspended in 100 µl NanoLuc Assay solution (Promega corp. Madison, WI, USA, cat# 1120). Homogenized solutions were transferred to white flat-bottom 96-well plates (Greiner Bio-One ...
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bioRxiv - Microbiology 2024Quote: ... difficile was mixed with 50 µL kit reagents (100-fold diluted LgBiT protein, 50-fold diluted substrate in kit buffer, Promega). Subsequently ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was digested overnight at 37 °C with sequencing-grade trypsin or chymotrypsin (Promega; 1:100 protease:protein weight ratio). After desalting on a C18 column (Pierce ...
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bioRxiv - Cancer Biology 2023Quote: ... Luciferase assays were performed by co-transfecting HEK-293T cells with 100 ng pCMV6-empty or pCMV6-miR-497/195 with 10 ng of psiCHECK2 reporter plasmids using FuGENE6 (E2691; Promega) and performing the Dual-Luciferase Reporter Assay System (E1910 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated at 37 °C for 24 h and then they were washed with 200 μL PBS per well and harvested in 100 μL per well of passive lysis buffer (Promega). Cells were freeze-thawed and 25 μL of lysate from each well was added to 25 μL of firefly luciferase reagent (Bright-Glo ...
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bioRxiv - Molecular Biology 2024Quote: ... Tail DNA from offspring was genotyped for the presence of the BAC transgene by analyzing 100 ng of tail DNA with the GoTaq PCR reaction kit (Promega) according to the manufacturer’s instruction by adding forward primer 5′-GAGGCTCGGGTGCGGCAGCTC −3′ and reverse primer 5′-GTGCAGTGTGAGCACCTGCTGTC-3′ for PCR with an initial denaturation for 3 min at 95 °C ...
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bioRxiv - Neuroscience 2024Quote: ... were calculated as the ratio of the fluorescence intensities between the initial (F0) and the last (F) 100 seconds by R-CEPIA1er in the Glomax Discover Microplate Reader (Promega). Caffeine-induced Ca2+ transients were also measured with Fura2 AM at room temperature using an ECLIPSE Ti/L100 epifluorescence microscope (Nikon ...
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bioRxiv - Cell Biology 2024Quote: ... The protein pellets were resuspended in 50 μl 100 mM TEAB and digested with 200 ng trypsin (Promega sequencing grade) overnight at 37°C.
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Phospholipase C β4 promotes RANKL-dependent osteoclastogenesis by interacting with MKK3 and p38 MAPKbioRxiv - Cell Biology 2024Quote: ... the existing media was removed and replaced with 100 µL of medium containing 20 µL of CellTiter 96 AQueous One solution (Promega). The cells were then incubated for 4 h at 37°C in a humified atmosphere with 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... dilute with 4 volumes of 20 mM Tris-HCl pH8 / 2 mM CaCl2 before overnight digestion at room temperature with 100 ng sequencing grade trypsin (Promega). Samples were centrifuged ...
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bioRxiv - Bioengineering 2024Quote: ... the 1X Renilla-luciferase substrate mixed with a Stop & Glo buffer (100 µL/well, Promega Dual Glo kit, cat # E1960) was added ...
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bioRxiv - Biochemistry 2024Quote: ... beads were resuspended in 100 μL of 50 mM TEAB and enzymatically digested overnight using sequencing-grade trypsin (Promega, V5111). Digested peptides were eluted through centrifugation and labeled using commercially available TMTsixplex tags (ThermoFisher ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were incubated for 15 hours and then washed with PBS and lysed using 100 μL of reporter lysis buffer (Promega). To measure luciferase activity ...
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bioRxiv - Cancer Biology 2024Quote: ... Integrated sgRNAs in each lymphoma sample were amplified from 100 ng of genomic DNA using GoTaq Green Master Mix (Promega) and indexing primers with unique overhangs (12) ...
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bioRxiv - Cell Biology 2020Quote: ... total RNA was annealed with 0.1 mg/ml oligo(dT)15 primer (Promega) and cDNA synthesis was performed with M-MLV Reverse Transcriptase (Promega ...
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bioRxiv - Microbiology 2020Quote: ... and digested in 10 µg/ml Trypsin Gold (Mass Spectrometry Grade, Promega, V5280) overnight at RT ...
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bioRxiv - Cell Biology 2022Quote: ... and suspended LysC solution (Promega, 12.5ng/mL in 25mM Tris-HCl pH 8.5). Digestion was performed overnight at 37°C ...