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Citations for Promega :
1251 - 1300 of 1604 citations for Phthalic Acid Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 2 μL of furimazine substrate was added to the reaction well from a working reagent stock of a 2:100 NanoDLR Stop & Glo Substrate to Buffer ratio (Promega #N1610). A total well volume of approximately 112.5 μL was incubated at room temperature for 2 minutes prior to measuring luminescence and OD600 readings on an EnVision plate reader (PerkinElmer).
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bioRxiv - Immunology 2023Quote: ... for 90 min at 37°C (enzyme-to-substrate ratio of 1:100) and subsequently digested overnight at 37°C with trypsin (Trypsin Gold, Mass Spec Grade, Promega, V5280) at an enzyme-to-substrate ratio of 1:50 ...
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bioRxiv - Microbiology 2023Quote: 1 ml of recombinant His-MBP-AlpA and His-MBP at a concentration of 10 μM was incubated with 100 μl MagneHis™ Ni Particles (Promega) equilibrated with pulldown-buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2023Quote: ... Homogenates were diluted 1/100 with the extraction buffer and mixed with the luminescent solution (CellTiter-Glo Luminescent Cell Viability Assay (Promega, RRID:SCR_006724)) ...
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bioRxiv - Genomics 2023Quote: ... The lysates were generated by adding 100 µL 1X Passive Lysis Buffer included in the Dual-luciferases assay kit (Promega E1960) to the protoplasts and vigorously shaking at room temperature for 15 minutes ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the treatment was removed from the wells and the cells were incubated with 100 μl of EGM™-2 complete medium with 20 mM HEPES and 20 μl of MTS (Promega, CellTiter 96® AQueous One Solution Reagent ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were lysed in 100 μl lysis buffer and subjected to promoter activity assay using the dual luciferase reporter assay system (Promega, USA). The luciferase enzymatic activity was measured using a PE Enspire Multilabel Reader (PerkinElmer ...
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bioRxiv - Cancer Biology 2024Quote: ... Northern blot was performed using poly(A)+ mRNA isolated from 100 µg of total CaSki RNA with PolyATtract mRNA Isolation Systems (Promega, #Z5310) or 10 µg of total CaSki RNA as described previously 8 ...
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bioRxiv - Cell Biology 2023Quote: ... The cell pellet was resuspended in 100 μl fresh TDB and HaloTag ligand (either in-house labelled Alexa647 ligand or Halo-Tetramethylrhodamine (TMR, Promega, USA) was added to a final concentration of 500 nM (Alexa647 ...
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bioRxiv - Biochemistry 2021Quote: ... 40 ng ml-1 of Renilla luciferase under a constitutive promoter (pRL-TK, Promega), and 40 ng ml-1 of pLEXm vector (34 ...
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bioRxiv - Biochemistry 2020Quote: ... HEK-293 PDE4A4 cell media is further supplemented with 500 μg/ml G418 (Promega). Culture conditions were 37°C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... which were destained and overnight-digested with trypsin (1 μg/mL, sequencing grade, Promega), before MS analysis of the peptide mixes ...
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bioRxiv - Bioengineering 2021Quote: ... The bottom of the chamber was coated with 10 μg ml−1 streptavidin (Promega) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... pH 7.2) and after adding 10 μL of 0.1 mg/mL GluC protease (Promega) incubated overnight at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Digestion was performed by adding 10 μL of 0.2 mg/mL Trypsin Gold (Promega) and overnight incubation at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... and dissociated in 50% Ham’s-12/DMEM medium containing 0.075 mg/mL liberase (Promega) for 30 minutes at RT ...
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bioRxiv - Microbiology 2020Quote: ... for 1 h at 55°C then with 200 μg/mL proteinase K (Promega) for 1 h at 55°C ...
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bioRxiv - Pathology 2021Quote: ... Antigen retrieval was performed by incubation with 20 μg/mL proteinase K (Promega, USA) for 20 minutes ...
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bioRxiv - Immunology 2022Quote: ... and rehydrated in 50 mM NH4HCO3 containing 10 μg / ml trypsin (sequence grade; Promega). After incubation overnight at 37 °C peptides were extracted and collected in a new tube ...
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bioRxiv - Neuroscience 2022Quote: ... The gel pieces were rehydrated in 60 μL of 20 μg/mL Trypsin (Promega) in 50 mM ammonium bicarbonate solution and incubated for 2h at 55°C ...
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bioRxiv - Microbiology 2023Quote: ... DNA samples were treated with a final concentration of 0.2mg/ml RNaseA (Promega #A7973) and 0.2mg/ml Proteinase K (NEB #P8107S ...
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bioRxiv - Immunology 2023Quote: ... droplets were resuspended in 30 ml of room temperature 6X SSC (Promega, Madison, WI) and 1 ml perfluorooctanol (Sigma-Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... grown up overnight in a 500 ml culture and maxiprepped (PureYield Maxiprep Kit, Promega). 400 μg of calmodulin and myosin-5 expression vectors (at a 1:6 ratio ...
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bioRxiv - Cell Biology 2023Quote: ... gel pieces were dried and rehydrated with 12.5 ng/mL trypsin (Promega, Madison, WI) solution in 25 mM ammonium bicarbonate on ice for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... Mice were intraperitoneally injected with 200 μl of 40 mg/ml D-luciferin (Promega) in PBS and anesthetized with isoflurane ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... We rehydrated slices with 12.5 μg/ml modified porcine trypsin (Promega, Madison, WI, USA) in 50 mM AMBIC and digested overnight at 37°C ...
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bioRxiv - Plant Biology 2019Quote: ... The luminescence of Luciferase was quenched and Renilla luminescence measured by the addition of 100 µL of Stop & Glo® Buffer (the Dual-Luciferase® Reporter Assay System, Promega). Luminescence was measured in technical triplicates for all combinations of transfected plasmids and each transfection was repeated thrice ...
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bioRxiv - Microbiology 2020Quote: Total RNA was extracted from 100 μl culture supernatants or apical washes using Monarch® Total RNA Miniprep Kit (Promega, Cat#: T2010S). For viral copy number quantification droplet digital PCR technology was applied (Bio-Rad Laboratories Inc ...
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bioRxiv - Microbiology 2021Quote: RNA was extracted from 50–100 mg of EDTA blood or 20 mg of liver using the SimplyRNA Tissue kit and Maxwell RSC16 instrument (Promega, Wisconsin, USA), as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation with 100 μL 50mM ammonium bicarbonate (MilliporeSigma Cat. #A6141) addition of 1 μg trypsin/lysC mix (Promega, Cat. # V5071) at 37 °C for 16 hours ...
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bioRxiv - Neuroscience 2022Quote: ... we transferred the beads to 100 µL 50 mM Tris-HCl buffer with mixed with 1 µg trypsin/Lys-C (Promega Cat# V5073) per replicate ...
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bioRxiv - Microbiology 2022Quote: ... The resulting materials were used as template to generate cDNA using 100 U Moloney murine leukemia virus (MMLV) reverse transcriptase (Promega, Madison, WI), 0.45 μM N6 random hexamer primers (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... The medium was then replaced with fresh medium in the incubator and protein release was induced by biotin addition (100 µM final) and following Furmazin (Promega, 1/500) addition ...
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bioRxiv - Microbiology 2020Quote: ... cells were lysed in 100 μL of passive lysis buffer and 50 μL lysate was incubated with 100 μL of luciferase assay substrate according to the manufacturer’s instructions (Promega, Madison, WI, USA).
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bioRxiv - Microbiology 2021Quote: Cells were washed with 1X Dulbecco’s PBS and harvested with 100 μL of 1X passive lysis buffer diluted in ddH2O (Promega, Madison WI, USA). Luciferase activity in cell lysates were measured by using either Firefly or Renilla luciferase kits (Promega ...
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bioRxiv - Biochemistry 2019Quote: ... A total of 100 µg of each protein sample was digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, Wisconsin, USA) by using filter-aided sample preparation (FASP ...
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bioRxiv - Microbiology 2021Quote: ... the cells were lysed in 100 μL of passive lysis buffer and 50 μL lysate was incubated with 100 μL of luciferase assay substrate according to the manufacturer’s instructions (Promega, Madison, WI, USA).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The resulting formazan precipitate was then dissolved by addition of 100 μL DMSO and quantified spectrophotometrically at 560nm using the GloMax Multi Microplate Multimode Reader (Promega, Madison, USA). Cell viability was expressed as a percentage relative to the negative control of untreated cell samples.
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bioRxiv - Molecular Biology 2023Quote: ... diluted with 4 volumes of 20 mM Tris-HCl pH 8 2 mM CaCl2 prior to overnight digestion with 100 ng sequencing grade trypsin (Promega, Cat# V5111) at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Two aliquots of 100 µL were transferred into new tubes and digested either with a sequencing-grade trypsin (Promega, Madison, WI, USA) or chymotrypsin (Promega ...
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bioRxiv - Systems Biology 2023Quote: ... and digested using LysC 1:100 enzyme:proteins ratio for 4 hours (Wako sequencing grade, 125-05061) and trypsin 1:100 enzyme:proteins ratio for 16 hours (Promega sequencing grade, V5111). The digested proteins were then acidified with 10% (v/v ...
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bioRxiv - Biochemistry 2023Quote: ... All samples were then diluted 8-fold with 100 mM NH4HCO3 and 1 mM CaCl2 and digested with sequencing-grade modified porcine trypsin (Promega, WI, USA) provided at a 1 ...
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bioRxiv - Genomics 2022Quote: ... The samples were diluted 1:1 with milliQ water and were incubated with a 1:100 w/w amount of trypsin (Promega, sequencing grade) overnight at 37 °C ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested using LysC 1:100 enzyme:proteins ratio for 4 hours (Wako sequencing grade, 125-05061) and trypsin 1:100 enzyme:proteins ratio for 16 hours (Promega sequencing grade, V5111). The digested proteins were then acidified with 10% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... mEPCs were incubated with 100 nM SiR-tubulin (Spirochrome, SC002) for 30 min or 200 nM Janelia Fluor® 549 HaloTag ligand (Promega, GA1110) for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The clear supernatant was incubated overnight at 4°C with 100 μl of prewashed Magne® HaloTag® Beads (Promega, WI, USA). Post-incubation ...
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bioRxiv - Microbiology 2023Quote: ... 100 μl of supernatant was removed from all wells and replaced with 100 μl of Bright-Glo luciferase substrate reagent (Promega; catalog #E2610). After 2 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... In-solution digestion was performed with LysC-Trypsin mix (1:100 enzyme: protein ratio) and trypsin (Promega; 1:50 enzyme: protein ratio). Peptides were desalted on C18 stage tips ...
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bioRxiv - Cancer Biology 2021Quote: ... and then resuspended in 4 ml DMEM/F12 + 80 μl DNase (1U/μl) (Promega M6101). The DNase solution was gently shaken by hand for 2–5 min ...
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bioRxiv - Microbiology 2021Quote: ... The lungs were incubated with 1 mg/mL of D-luciferin (Promega, Madison, WI, USA) for 5 min ...