Labshake search
Citations for Promega :
1201 - 1250 of 1604 citations for Phthalic Acid Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2020Quote: ... ATP (0.2 mM) and/or protein kinase A (0.1 mg/mL; PKA, Promega) were then added to hydrogel droplets for 12 hours at 25 °C ...
-
bioRxiv - Cell Biology 2019Quote: ... gel pieces were dried and rehydrated with 12.5ng/mL trypsin (Promega, Madison, WI) solution in 25mM ammonium bicarbonate on ice for 30min ...
-
bioRxiv - Microbiology 2021Quote: ... cells were lysed in 200 ml Dual Luciferase Passive Lysis Buffer (Kit Promega) and both Luciferase (FF-Luc ...
-
bioRxiv - Pathology 2021Quote: ... pre-treated with DNAse (50u/ml, 15min RT, RQ1 RNAase free DNAse, Promega) and cytoplasmic fractions were adjusted ...
-
bioRxiv - Developmental Biology 2021Quote: ... and 175 μg/ml 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) (Promega). Reactions were stopped with three PBS rinses ...
-
bioRxiv - Plant Biology 2020Quote: ... One mL of 1X cell lysis buffer from the luciferase assay system (Promega) was added to each sample ...
-
bioRxiv - Genomics 2021Quote: ... Sorted cells were then unfixed overnight in 50 μg/mL proteinase K (Promega) plus 150 mM NaCl at 65°C ...
-
bioRxiv - Developmental Biology 2022Quote: ... and 175 μg/ml 5-bromo-4-chloro-3- indolyl-phosphate (BCIP) (Promega). Reactions were stopped as the signal became apparent with three PBS rinses ...
-
bioRxiv - Neuroscience 2022Quote: ... and recombinant RNasin ribonuclease inhibitor (1 uL/ml; Promega, Madison, WI; Cat#N2511). These washes were followed by an additional wash with 500 μl each of both wash buffer and high salt wash buffer ...
-
bioRxiv - Neuroscience 2022Quote: ... and recombinant RNasin ribonuclease inhibitor (1 ul/ml; Promega, Madison, WI; Cat#N2511) with a mechanical homogenizer followed by addition of TURBO DNase (2 μl ...
-
bioRxiv - Systems Biology 2023Quote: ... 0.5ul “home-made” Tn5 transposase adapter complex and 0.4mg/ml digitonin (Promega, #G9441) at 30 min at 37 °C ...
-
bioRxiv - Biochemistry 2023Quote: ... one of which was incubated with 50 µg /ml of RNase A (Promega) at 37 °C for 30 min ...
-
bioRxiv - Genetics 2023Quote: ... and 0.45% Tween-20) with Proteinase K (20mg/ml, Promega, Cat. No. MC5005) added just before use ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 10 μL/ml RNasin® Plus Ribonuclease Inhibitor (Promega, Madison, WI, USA). The tissue lysates were then homogenized on ice and 1% of each lysate volume was separated as Input samples ...
-
bioRxiv - Biochemistry 2024Quote: ... The remainder of the reaction was treated with 50 µg/mL trypsin (Promega) and incubated at room temperature for the indicated times ...
-
bioRxiv - Cancer Biology 2021Quote: Trypsin digestion was carried out overnight at 37 °C with 1:50-1:100 enzyme– protein ratio of sequencing grade-modified trypsin (Promega V5111) in 50 mM NH4HCO3 pH 7.5 ...
-
bioRxiv - Biochemistry 2020Quote: ... Samples were diluted 10-fold with 100 mM Tris (pH 8.0) and incubated overnight at 37°C with sequencing grade trypsin (Promega, Madison, WI) added at a 1:50 enzyme:substrate ratio (wt/wt) ...
-
bioRxiv - Systems Biology 2019Quote: ... The sample was diluted with 100 mM tris to a final urea concentration of 1.5 M urea and digested with trypsin (Promega, Madison, WI) overnight at room temperature (1:50 ...
-
bioRxiv - Bioengineering 2019Quote: ... using a reaction solution consisting of RNA (100 or 200 ng) supplemented with 1 μL random primers (50 ng μL−1, Promega, C1181), 1 μL dNTPs (10 mM ...
-
bioRxiv - Biochemistry 2021Quote: ... Cells were then co-transfected with 100 ng luciferase vector and 20 ng of pGL4-hRLuc-TK (Renilla, Promega, Madison, WI). 24 hours later ...
-
bioRxiv - Microbiology 2020Quote: HPV infected or pGL3 transfected cells were washed once with PBS and lysed in 100 μL reporter lysis buffer (Promega E3971). Luciferase activity was measured on a DTX-800 multimode plate reader (Beckman Coulter ...
-
bioRxiv - Cell Biology 2020Quote: ... with a Tris/NaCl buffer (100 mM Tris and 75 mM NaCl at pH 8) containing 1 μg LysC (Promega, Va11A) per sample ...
-
bioRxiv - Neuroscience 2022Quote: ... The standard curve was generated by cloning a short 100-bp sequence of mitochondrial 16S rRNA gene into pGEM®-T Easy Vector (Promega). The 16S_rRNA gene sequence was amplified with Taq Polymerase (Supreme NZYtech II master mix ...
-
bioRxiv - Microbiology 2021Quote: ... Virus-mAb-containing media was then aspirated from cells and 100 μL of a 1:4 dilution of Bio-glo (Promega, G7940) in PBS was added to cells ...
-
bioRxiv - Neuroscience 2020Quote: ... total RNA was placed in 4 µl of lysis buffer (0.1% vol/vol Triton X-100, 2.5 mM dNTPs, 2.5 µM oligo-dT, 1 U/µl Promega RNasin Plus RNase inhibitor). Reverse transcription was performed followed by cDNA amplification ...
-
bioRxiv - Plant Biology 2021Quote: ... and 100 μl sample volumes were assayed in triplicate for ATP content using the BacTiter-Glo bioluminescence kit (Promega, Mannheim, Germany). After 5 min incubation in the dark to allow for the decay of plate autofluorescence ...
-
bioRxiv - Neuroscience 2021Quote: ... Further 50ug in 100 µl protein sample was added into vials and for ATP measurement luminescence was measured through luminometer (Promega glowmax).
-
bioRxiv - Biochemistry 2020Quote: ... The RNA was added to the 100 µl SA Sepharose slurry together with 1 µl RNasin Plus RNase inhibitor (40 U/µL, Promega, N261A). 10 µl of the supernatant was saved for extraction of input RNA using TRIzol (Invitrogen ...
-
bioRxiv - Bioengineering 2020Quote: ... 100 μl of each supernatant were transferred into new 96 well plates and equal volume of BacTiter-Glo™ Reagent (Promega) was added per well ...
-
bioRxiv - Immunology 2022Quote: ... Samples were diluted two-fold with 100 mM of triethylammonium bicarbonate (TEAB) and proteins were digested during 3 hours with 1 µg of trypsin (Promega V5111) and 1 µg of LysC (Wako 129-02541 ...
-
bioRxiv - Microbiology 2022Quote: ... washing two times with 100 µl PBS and then lysed with 1x lysis buffer (made from 5x Promega Firefly Lysis Buffer). Plates were frozen for at least 24 hours and then thawed for 2 hours before analyzing with Promega Firefly Luciferase Kit on a luminometer.
-
bioRxiv - Microbiology 2020Quote: ... After 24 hours of growth luciferase activity was visualized by spraying one spray of 1:100 reconstituted NanoGlo Luciferase substrate (Promega N1110) per agar plate using a disposable spray flask ...
-
bioRxiv - Epidemiology 2019Quote: ... A band of about 350 base pairs (bp) in size was confirmed using a Ready-LoadTM 100 bp DNA ladder (Promega, USA). We submitted the positive PCR products for sequencing to Macrogen Inc (Seoul ...
-
bioRxiv - Genetics 2021Quote: ... 5 μL of substrate was added to a final concentration of 100 nM 11S fragment and 0.5X furimazine (Promega, NanoGlo Substrate). Luminescence was measured on a ViewLux HTS reader (PerkinElmer ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Infected SupT1 cells were cultured for another 48 h and directly lysed with 100 uL of Bright-Glo Luciferase Reagent (Promega, #E2610). Cell lysate was measured for luciferase activity using a LUMIstar Omega microplate luminometer (BMG Labtech) ...
-
bioRxiv - Biochemistry 2020Quote: ... Proteins were then reduced with 10 mM DTT in 100 mM Ambic for 30 min at 40 °C and alkylated with 55 mM iodoacetamide in 100 mM Ambic for 20 min at 40 °C followed by digestion with 0.3 μg trypsin (sequence grade, Promega, Madison, WI) in 50 mM Ambic for 5 h at 40 °C ...
-
Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... MBP-LIC1 (50 pmol) and either BICD2N-Halo (50 pmol) or CBD-HAP1CC1 (100 pmol) was incubated with resin (Magne® HaloTag, Promega, G7281 ...
-
bioRxiv - Immunology 2021Quote: ... 20 Nb L3 were collected with 100 μl of PBS and 100μl of the Celltiter-Glo Luminescent reagent (Promega, Madison, WI) was then added ...
-
bioRxiv - Cancer Biology 2022Quote: ... and the appropriate protein amount (60 μg for baseline expression experiments and 1mg for perturbation experiments) was digested with LysC (1:100, Wako, #121-05063) for 4 h and Trypsin (1:75, Promega, #V5113) overnight ...
-
Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... The urea concentration was reduced to <2M and the protein sample was digested overnight at 37°C with 1:100 Trypsin Gold (Promega V5280). The following day ...
-
bioRxiv - Immunology 2022Quote: ... Proteins then were reduced with 10 mM DTT in 100 mM Ambic for 30 min at 40oC and alkylated with 55 mM iodoacetamide in 100 mM Ambic for 20 min at 4°C followed by digestion with 0.3 mg trypsin (sequence grade, Promega, Madison, WI) in 50 mM Ambic for 5 h at 4°C ...
-
bioRxiv - Immunology 2022Quote: ... Loss of cell viability reflecting the efficiency of viral infection was measured 3 days after infection by adding 100 μL Cell Titer-Glo reagent to each well (CellTiterGlo Luminescent Cell Viability Assay, Promega #G7571), according to the manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2022Quote: ... 6.5 μl of 1:100 diluted cDNA per reaction were used with the GoTaq® qPCR Mastermix (Promega GmbH, Madison, USA) in a total volume of 15 μl ...
-
bioRxiv - Molecular Biology 2022Quote: ... Proteins then were reduced with 10 mM DTT in 100 mM Ambic for 30 min at 40°C and alkylated with 55 mM iodoacetamide in 100 mM Ambic for 20 min at 40°C followed by digestion with 0.3 μg trypsin (sequence grade, Promega, Madison, WI) in 50 mM Ambic for 5 hours at 40°C ...
-
bioRxiv - Microbiology 2023Quote: ABIL emulsion oil was prepared by supplementing mineral oil with 4% ABIL EM 90 (Redox, Australia) and 0.05% Triton X-100 (Promega, United States). 425 μL of ABIL oil was added to the PCR mix ...
-
bioRxiv - Molecular Biology 2023Quote: ... at an enzyme-to-substrate ratio of 1:100 and subsequently digested overnight at 37°C with sequencing grade trypsin (Trypsin Gold, Mass Spec Grade, Promega, V5280) at an enzyme-to-substrate ratio of 1:50 ...
-
bioRxiv - Molecular Biology 2023Quote: ... The semisoft pellet of worms was weighed to ensure an equal number of worms in each sample (100 µg of worm pellet per sample) and mixed with 800 µL of 1× passive lysis buffer (Promega, #E1910). This was followed by eight cycles of sonication with 3 s on and 30 s off at an amplitude of 40% ...
-
bioRxiv - Biochemistry 2023Quote: ... 25 μL diluted lysate was mixed with an equal volume of Renilla-Glo Luciferase Assay System (prepared 1:100 as recommended; Promega # E2710) for 10 min at room temperature.
-
bioRxiv - Bioengineering 2023Quote: ... 1 well-1 with 100 μL fresh cell culture media) and mixed with 100 μL reaction solution of CellTiter-Glo 3D cell viability assay kit (G9681; Promega, WI). The 96-well plate was gently rocked for 5 min and stabilized at room temperature for 30 min ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were pretreated with indicated antagonist for 1hr before treatment with 10 nM of PGF2a or 100 nM PGE2 overnight before glucose uptake was measured by Glucose Uptake-Glo™ Assay kit (Promega) according to the manufacturer’s instructions.