Labshake search
Citations for Promega :
1351 - 1400 of 1604 citations for Phthalic Acid Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2022Quote: ... 20 µL of PBS containing 10 μg/mL Hoechst and 1/4000 CellTox Green Dye (Promega) were dispensed per well 8 h prior to imaging at Opera Phenix (Perkin Elmer ...
-
bioRxiv - Genetics 2022Quote: ... Supernatants were discarded and the remaining 0.2 mL were digested with DNases (Promega and Epicentre, USA), RNase A (Ambion ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Fifty microliters of D-luciferin (30 mg/mL in saline) was injected subcutaneously in mice (Promega). Twenty minutes after the injection ...
-
bioRxiv - Plant Biology 2022Quote: ... and GST- GBF3 were purified from 4 ml culture and immobilized on MagneGST Glutathione particles (Promega). Briefly ...
-
bioRxiv - Cell Biology 2023Quote: ... were analyzed with the addition of luciferin (100mg/mL) or furimazine (Nano-Glo® Luciferase, Promega) diluted 1:200 to 1:500 in 1X PBS ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were incubated in 1.5 mL per well of 20 nM TMR HaloTag ligand (Promega, G8251) in pre-warmed medium for 15 minutes ...
-
bioRxiv - Systems Biology 2023Quote: ... Half of the lysis was treated with 5 µl of 10 mg/ml Rnase (Promega:527491) and both lystates (+/- RNase ...
-
bioRxiv - Biochemistry 2023Quote: ... and sequencing grade trypsin (10 μL, 0.5 mg/mL in 50 mM ammonium bicarbonate, Promega, V5111) were added for overnight incubation at 37 °C ...
-
bioRxiv - Cancer Biology 2023Quote: ... organoids were stained using Hoechst 33342 (1 μg/mL) and CellTox Green (Promega, 1/20 000) dyes for 8 h prior to imaging at Opera Phenix (Perkin Elmer ...
-
bioRxiv - Biochemistry 2023Quote: ... 10 μl of NanoBRET™ Nano-Glo Substrate solution (8 μl/ml Nano-Glo substrate, Promega, diluted in DMEM/F12 no phenol red ...
-
bioRxiv - Genomics 2023Quote: ... 500 μl of CTAB lysis buffer and 10 μl of Proteinase K (20 mg/ml; Promega) were added to the sample and incubated at 65ºC for 90 minutes ...
-
bioRxiv - Cancer Biology 2023Quote: ... mice received intraperitoneal injection of 50 μl 30 mg/ml d-luciferin (in PBS) (E1603, Promega). Continuous administration of isofluorane gas was provided to maintain anaesthesia of animals during imaging ...
-
bioRxiv - Systems Biology 2024Quote: ... After 48 hours each plate was treated with 25 mL’s of CellTiter Glo (Promega Corp G7572) and read in enhanced luminescence mode on an EnVision Multi-Label plate reader (Revvity Corp.) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in 1 mL of the same buffer containing protease inhibitor cocktail (Promega, USA), and then transferred to 2 mL lysing matrix B tubes containing 0.1 mm diameter silica beads (MP Biomedicals ...
-
bioRxiv - Microbiology 2024Quote: ... The only exceptions being the use of 2.5 mL of 10x FastBreak Cell Lysis Reagent (Promega) to lyse the 1 L cell pellets and the Gel Filtration Buffer (25 mM Tris-HCl pH 8 ...
-
bioRxiv - Molecular Biology 2024Quote: ... The samples underwent overnight on-bead digestion with 3.5 µl of trypsin (0.1 mg/mL, Promega) and were dissolved in 50 mM acetic acid in elution buffer ...
-
bioRxiv - Genomics 2020Quote: ... Next nuclei were pelleted and the transposition reaction was performed incubating the lysate for 30 min at 37 °C under agitation in the presence of Transposition mixture (Tris-HCl pH 7.6 10 mM, MgCl2 5 mM, dimethyl formamide 10%, Tn5 enzyme 100 nM – Illumina #20018704, digitonin 0.01% - Promega #G9441, Tween-20 0.1%, PBS 33%). The transposition reaction was purified (Qiagen MinElute PCR Purification kit ...
-
bioRxiv - Immunology 2022Quote: ... and digested over-night with Lys-C-Trypsin mix (1:100 enzyme to protein ratio) and trypsin (Promega; 1:50 enzyme to protein ratio). Following the digestion step ...
-
bioRxiv - Cell Biology 2020Quote: ... samples were blocked in 20% normal goat serum (NGS) for 1 h and incubated with the HaloTag® polyclonal anti-rabbit (Promega, 100-fold dilution) in PBS supplemented with 2% NGS overnight at 4°C ...
-
bioRxiv - Cancer Biology 2024Quote: ... and pellets were washed twice with ice-cold ethanol followed by overnight incubation at 37°C in 100 mM ABC containing 1 mM CaCl2 and trypsin (Sequencing grade, modified, Promega Co, Madison, WI, USA). Digested proteins were evaporated and each sample was freed from salts and buffers by solid-phase extraction on C18 resin using cartridges prepared in-house ...
-
bioRxiv - Immunology 2020Quote: ... For the luciferase assay we mixed 20 μl of those supernatants with 100 μl of the luciferase assay reagent (Promega, Luciferase Assay System Kit #E1500) in a well of a white ...
-
bioRxiv - Molecular Biology 2019Quote: ... for 30 minutes at room temperature and then digested by LysC (Lysyl endopeptidase, #125-05061, Wako, ratio LysC/protein 1:100) and trypsin (Trypsin Gold, V5280, Promega, ratio trypsin/protein 1:20) overnight ...
-
bioRxiv - Molecular Biology 2023Quote: 100 µg of total RNA was used to perform poly(A)+ RNA isolation using PolyATtract® mRNA Isolation Systems (Promega UK LTD, Cat. No. Z5310). 750 ng of recovered mRNA was used for library preparation using the Direct RNA Sequencing kit following the manufacturer’s instructions (Oxford Nanopore Technologies ...
-
bioRxiv - Biophysics 2023Quote: ... The 12-230 kDa separation module was used for PCNA (CST, D3H8P, Rabbit, 1:100 dilution, #13110), b-Actin (CST, D6A8, Rabbit, 1:50 dilution, #8457S) and Halo (Promega, Mouse, 1:10 dilution, #G9211) detection ...
-
bioRxiv - Biochemistry 2019Quote: ... Protein was digested by incubation overnight at 37°C with trypsin (5 ng/ ml, Promega, Madison, WI) in 50 mM NH4HCO3 (pH 7.8) ...
-
bioRxiv - Cell Biology 2021Quote: ... lysed in lysis buffer (20mM Tris-Cl, pH 7.5, 150mM KCl, 5mM MgCl2, 100U/ml RNasin [Promega, Madison ...
-
bioRxiv - Plant Biology 2021Quote: ... Selection was done twice for 2 weeks on medium containing 25 µg/ml G418 (Promega, Mannheim, Germany) starting 2 weeks after transformation with a 2-weeks release period in between ...
-
bioRxiv - Cell Biology 2021Quote: ... The soluble fraction was incubated with 2.5 ml HaloLink resin slurry (Promega, pre-equilibrated in Halo buffer) and allowed to incubate on a roller for 90 min at room temperature ...
-
bioRxiv - Developmental Biology 2022Quote: ... The cell pellet (on ice) was resuspended in 3 ml Red Blood Cell Lysis Solution (Promega, Z3141) for 4 min and span down for 5 min at 300g in a pre-cooled centrifuge.
-
bioRxiv - Microbiology 2020Quote: ... membranes were incubated in a 0.165 mg/ml BCIP (5-bromo-4-chloro-3-indolyl phosphate, Promega)/ 0.33 mg/ml NBT (p-nitroblue tetrazolium chloride ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Bacterial DNA was extracted from 1 ml of culture using the Wizard genomic DNA purification kit (Promega). Evolved population DNAs were sequenced at the GeT-PlaGe core facility (https://get.genotoul.fr/) ...
-
bioRxiv - Cell Biology 2022Quote: ... a sheep anti-BrdU antibody was used in the presence of RQ1 DNase (Promega, 10 units/ml). and after washing with conjugated anti-sheep antibodies (see antibodies section) ...
-
bioRxiv - Neuroscience 2019Quote: Genomic DNA extracted from 3 ml fresh EDTA-blood (wizardR Genomic DNA purification Kit, Promega, Madison, WI) was used for genotyping ...
-
bioRxiv - Biochemistry 2020Quote: ... Destained gel pieces were dried in vacuo and rehydrated by the 12.5 μg/ml trypsin (Promega, USA), 50 mM ABC ...
-
bioRxiv - Molecular Biology 2019Quote: ... tissue was immediately incubated in 1% PFA (with 1 µL mL−1 RNasin Plus (Promega, cat. #N2611)) for 5 minutes at 4°C ...
-
bioRxiv - Microbiology 2019Quote: ... 1 mL of culture was used for genomic DNA isolation with the Wizard DNA Purification Kit (Promega). Approximately 2 µg of DNA was used to prepare a paired-end 250-bp sequencing library with the Nextera XT DNA library kit ...
-
bioRxiv - Molecular Biology 2023Quote: ... each TECprobe-ML reaction was 48 μl due to the omission of 10X (1 μM) streptavidin (Promega) and 10X Start Solution [100 mM MgCl2 ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Samples serving as positive controls for the cytotoxicity measurement received 72 μg/mL membrane-permeabilizer digitonin (Promega) in respective ToxGlo Media type (glucose or galactose) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins were digested via the addition of 5 μL of sequencing grade trypsin (0.1 mg/mL) (Promega) for 3 h at 37 °C ...
-
bioRxiv - Bioengineering 2023Quote: ... samples were incubated overnight at 37°C with sequencing grade modified trypsin (0.01 mg/mL; Promega Corporation) to allow protein digestion ...
-
bioRxiv - Plant Biology 2023Quote: ... Plasmid purification from 50 mL bacterial cultures was made using PureYield™ Plasmid Midiprep System (Promega, A2492) for C ...
-
bioRxiv - Genetics 2023Quote: ... Additional 140 uL of lysis buffer and 40uL of proteinase K (20mg/ml, Promega, Cat. No. MC5005) were added ...
-
bioRxiv - Biochemistry 2023Quote: ... The trypsin digestion commenced with the addition of 8 μL Gold Trypsin (0.5 mg/mL Promega V5280) along with 160 μL of 50 mM Tris pH 8 and 2 μL 100 mM CaCl2 ...
-
bioRxiv - Biochemistry 2021Quote: ... free thiols were alkylated by iodoacetamide and proteins were sequentially digested with endoproteinase Lys-C (Wako, 1:100 enzyme-to-substrate ratio, 3 h at 37 °C) and trypsin (Promega, 1:50, overnight at 37 °C). Digested samples were desalted ...
-
bioRxiv - Cell Biology 2019Quote: Proteins were incubated overnight at 37°C with 20 µl of 25 mM NH4HCO3 containing sequencing-grade trypsin (12.5µg/ml, Promega) at 37°C ...
-
bioRxiv - Microbiology 2019Quote: ... swab tips were placed into DNA/DNase/RNase-free 1.5 ml Eppendorf tubes containing nuclease-free water (Promega). The swab shafts were cut with a pair of sterile dissection scissors to allow closing of the tubes ...
-
bioRxiv - Biochemistry 2019Quote: ... 2mL of Sf9 cells at 0.5×106 cells/mL were transfected with 2µg of fresh bacmid DNA and FuGene HD transfection reagent (Promega) at a 3:1 transfection reagent to DNA ratio according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: ... converted 1µg of DNase-treated RNA to cDNA with 20nt Oligo-dT (25µg/mL; IDT) and rRNAsin (Promega). Precipitated cDNA was resuspended in water at 20ng/µL of original RNA concentration ...
-
bioRxiv - Cancer Biology 2021Quote: ... the protein extracts were then incubated overnight at 37°C with 20 μl of 25mM NH4HCO3 containing sequencing-grade trypsin (12.5μg/ml, Promega). The resulting peptides were desalted using ZipTip µ-C18 Pipette Tips (Millipore ...
-
bioRxiv - Cell Biology 2021Quote: ... 2mL of Sf9 cells at 0.5×106 cells/mL were transfected with 2µg of fresh bacmid DNA and FuGene HD transfection reagent (Promega) at a 3:1 transfection reagent to DNA ratio according to the manufacturer’s instructions ...