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Citations for Promega :
1401 - 1450 of 1604 citations for Phthalic Acid Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... aliquots of the enzyme were removed from the freezer and were treated with thrombin (0.1 kU/mL, Promega) overnight at 4 °C in enzyme storage buffer (20 mM HEPES ...
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bioRxiv - Cancer Biology 2021Quote: ... The gel sections were lyophilized again before in-gel trypsinization (20 mg/mL sequencing grade modified trypsin, Promega) overnight at 37°C with gentle shaking ...
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bioRxiv - Molecular Biology 2022Quote: ... The urea concentration was then diluted with 17 μl of 50 mM HEPES pH 8.5 and after adding 1 μl of 0.2 mg/ml trypsin (Promega) in 0.2 mM HCl the digestion continued overnight at 37°C with interval mixing at 2000 RPM for 30 s every 15 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysis buffer (0.1% v/v NP-40, 0.1% v/v Tween-20, 0.1mg/mL Digitonin ((Promega, Southampton, UK) in resuspension buffer ...
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bioRxiv - Plant Biology 2021Quote: ... filled with 175 μL 0.5x MS-agar medium 1% sucrose and 35μL (50 μg/mL) of Beetle Luciferin (Promega) dissolved in DMSO ...
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bioRxiv - Biochemistry 2019Quote: ... DMEM media was aspirated from the cells and 500 µL of hypotonic lysis buffer (50 mM Tris.HCl pH 7.4, 25 units/mL DNase I (Promega), 0.2 % (v/v ...
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bioRxiv - Molecular Biology 2019Quote: ... and 200 μL of the cell/supernatant suspension was treated overnight with 80 μg/ml proteinase K (Promega) in 1x proteinase K digestion buffer (10 mM Tris-HCl pH 7.4 ...
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bioRxiv - Synthetic Biology 2020Quote: ... a 1 mL aliquot was taken for genomic isolation through the Maxwell kit (Promega, Cat# AS1490, Madison WI). Successful integration was verified through qPCR ...
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bioRxiv - Synthetic Biology 2020Quote: ... a 1 mL aliquot was taken for genomic isolation using the Maxwell kit (Promega, Cat# AS1490, Madison WI). Successful integration was verified using qPCR ...
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bioRxiv - Molecular Biology 2021Quote: ... The gel sections were lyophilized again before in-gel trypsinization (20 mg/mL sequencing grade modified trypsin, Promega) overnight at 37°C with gentle shaking ...
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bioRxiv - Biophysics 2019Quote: ... the beads were incubated in a PBS solution containing 25 μg/mL of the HaloTag ligand O4 (Promega) for at least 4 h at constant rotation (room temperature) ...
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bioRxiv - Neuroscience 2021Quote: ... gel pieces were rehydrated with 10 µL 50 mM NH4HCO3 containing 25 µg/mL trypsin (Trypsin Gold, Promega) and incubated for 15 min on ice ...
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bioRxiv - Cancer Biology 2021Quote: ... containing 2μl of lysis buffer (1.9ml of 0.2% v/v Triton-X l00 v/v + 0.1ml of RNasin Plus RNase inhibitor (10,000 U/ml, Promega). Smart-seq2 protocol was performed as described in Picelli et al [43] ...
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bioRxiv - Cell Biology 2021Quote: ... 2mL of Sf9 cells at 0.5×106 cells/mL were transfected with 2μg of fresh bacmid DNA and FuGene HD transfection reagent (Promega) at a 3:1 transfection reagent to DNA ratio according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... In-gel digestion was performed by adding 25 μL of 4 μg/mL sequencing grade modified trypsin (Promega) in 25 mM ammonium bicarbonate ...
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bioRxiv - Biophysics 2022Quote: ... The poly-L-lysine coated side of the cover slip was exposed to 5 mg/ml streptavidin (Promega) in this space for 15 min ...
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bioRxiv - Immunology 2022Quote: ... The samples were then subjected to in-gel trypsin digest (Trypsin Gold, Mass Spectrometry Grade. Promega. 20μg/ml). Samples were loaded in an UltiMate 3000 HPLC autosampler system (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: Total DNA was extracted from 10 ml bacterial overnight grown cultures using Wizard Genomic DNA Purification Kit (Promega) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... was resuspended in 1.5 mL salt-tris solution buffer supplemented with 0.2 U/ul RNasin Plus RNase Inhibitor (Promega). The tissue material was spin down at 500 x g for 5 min at 4°C ...
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bioRxiv - Genomics 2022Quote: ... was resuspended in 1.5 mL wash buffer (1x PBS, 1% BSA and 0.2U μl/1 RNasin Plus RNase Inhibitor (Promega)) ...
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bioRxiv - Microbiology 2023Quote: Mid-log phase fusobacterial cells (OD600 of ∼ 0.5) were harvested by centrifugation and combined in equal volumes (0.1 ml) with the BacTiter-Glo Reagent (Promega) in multi-well plates (Cellstar) ...
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bioRxiv - Molecular Biology 2023Quote: ... The gel sections were lyophilized again before in-gel trypsinization (20 mg/mL sequencing grade modified trypsin, Promega) overnight at 37°C with gentle shaking ...
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bioRxiv - Immunology 2023Quote: ... and cells were treated with 3µg/ml LPS O111:B4 and 0.25% (vol/vol) Fugene HD Plus (Promega) to cause transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... The samples were diluted with 17 µL of 50 mM HEPES (pH 8.5) and digested with 1 µL of 0.2 mg/mL trypsin (Promega) in 0.2 mM HCl at 37°C with interval mixing at 2000 RPM for 30 sec every 15 min.
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bioRxiv - Cancer Biology 2024Quote: ... then washed with 1 ml of PBS and lysed using 60 μl of Cell Culture Lysis Reagent (Promega). 20 μl of cell lysate was added to 20 μl of Luciferase Substrate (Promega) ...
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bioRxiv - Biochemistry 2021Quote: ... The dried resin was resuspended with 50 mM Tris pH 7.5 (2x resin volume) and 200 ng of trypsin was added (Promega; made up to 100 ng·μL-1 in H2O), the samples were digested overnight shaking at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... Samples were ground to fine powder using pestles on dry ice and then 100 μl 2x cell culture lysis buffer was added (Promega, E1531; with 150 mM Tris-Hcl pH7.5). 50 μl of leaf extract was mixed with 50 μl luciferase substrate (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... The samples were then diluted to 2 M urea final concentration via the addition of 20 mM HEPES pH 8.0 and digested overnight with 5 µg/ml sequence-modified trypsin (Promega) at room temperature.
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bioRxiv - Cell Biology 2022Quote: ... Beads were washed three times for 10 min with gentle rotation in high-salt buffer (50 mM Tris, pH 7.5, 300 mM KCl, 12 mM MgCl2, 1% Nonidet P-40, 1 mM DTT, 100U/mL Promega RNasin ...
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bioRxiv - Neuroscience 2019Quote: ... Thawed tissue was minced and incubated in 1% PFA (with 1 µL mL−1 RNasin Plus (Promega, Madison, WI)) for 5 minutes ...
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bioRxiv - Bioengineering 2019Quote: ... to 30 µL of 0.61 mM digitonin in a 1.5 mL microcentrifuge tube that had been pre-inserted in the luminometer reader (GloMax, Promega). The signal was recorded for 1 minute ...
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bioRxiv - Neuroscience 2019Quote: ... Three adult male heads were collected into a 1.5 ml Eppendorf tube and homogenized in 50 μl of Glo Lysis Buffer (E266A, Promega) at Zeitgeber time (ZT ...
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bioRxiv - Biophysics 2020Quote: ... Gel plugs were soaked in 50 mM ammonium bicarbonate (pH 7.8) containing 20 μg/mL of trypsin gold (Promega) for 24 hours at 37°C ...
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bioRxiv - Immunology 2021Quote: ... Single CD3-CD8-CD14-CD16-CD20+Ova-RBD-PE+RBD-AF647+ B cells were sorted into individual wells of 96-well plates containing 4 μl of lysis buffer (0.5× PBS, 10 mM DTT, 3,000 units/ml RNasin Ribonuclease Inhibitors (Promega, N2615) per well using a FACS Aria III and FACSDiva software (Becton Dickinson ...
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bioRxiv - Biochemistry 2020Quote: ... the samples were brought to room temperature for 5-10 minutes while 2 mL of 30 mM coelenterazine (Promega), or 15 mL of 1-to-50 diluted NanoLuc substrate (Promega) ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 mg/mL 50 μL aliquots of cleared lysates were incubated with 2 μL of indicated trypsin concentration (Promega) at 4°C for 15 min ...
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bioRxiv - Cell Biology 2021Quote: ... collected at 2000 x g followed by resuspension in 0.5 ml of storage buffer (PBS containing 4% BSA and RNasin (Promega) – 0.2 U/ul ...
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bioRxiv - Pathology 2021Quote: ... and 20 µg/mL L-012) and recorded by a Promega GloMax Navigator microplate luminometer (Promega Biotech, Beijing, China). GraphPad Prism software was used to analyze data.
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bioRxiv - Biochemistry 2022Quote: ... Cells were diluted to a final concentration of 500,000 cells/mL in Expi293 expression medium plus 2% (v/v) Glosensor reagent (Promega) and incubated for 75 minutes at room temperature with gentle rotation ...
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bioRxiv - Biophysics 2022Quote: ... and resuspended in assay buffer (HBSS with 20 mM HEPES pH 7.45) with 1 μg/mL freshly prepared coelenterazine h (Promega). Cells were plated in white-walled ...
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bioRxiv - Molecular Biology 2022Quote: ... Fresh bacmid DNA was transfected into Sf9 cells at 0.5×106 cells/mL in 6-well cell culture plates using FuGene HD (Promega) according to the manufacturer’s protocol (final concentration 10 μg/mL) ...
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bioRxiv - Immunology 2022Quote: ... Single CD3−CD8−CD14−CD16−CD20+Ova−Gamma NTD-PE+Wuhan-Hu-1 NTD-AF647+ B cells were sorted into individual wells of 96-well plates containing 4 μl of lysis buffer (0.5× PBS, 10 mM DTT, 3,000 units/ml RNasin Ribonuclease Inhibitors (Promega, N2615) per well using a FACS Aria III and FACSDiva software (Becton Dickinson ...
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bioRxiv - Microbiology 2020Quote: ... The proteins in the sample were digested with 10 μg/mL sequence grade modified trypsin (Promega, Madison, WI, USA) at 37°C overnight ...
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bioRxiv - Physiology 2022Quote: ... Cells were then air-dried for 30 min and lyse with 200 μg/ml proteinase K (Promega, WI, USA) in lysis buffer ...
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bioRxiv - Microbiology 2019Quote: ... The cells were then scraped into the media and the media + cells were digested with proteinase K (80μg/mL) (Promega) in 5x proteinase K digestion buffer (50mM Tris-HCl pH 7.4 ...
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bioRxiv - Cell Biology 2019Quote: ... proteins were trypsinised on the beads in 60 μl of Buffer I (2 M urea, 50 mM Tris-HCl pH 7.5, 5 μg/ml Trypsin [modified sequencing-grade trypsin; Promega]) for 30 min at 37°C in a thermomixer ...
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bioRxiv - Molecular Biology 2020Quote: ... Hygromycin (150ug/ml) was used to select for cells with integration and Nano-Glo Dual-luciferase assay kit (Promega) was used to confirm expression of both Firefly and Nano-luciferase in clonally isolated cells.
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bioRxiv - Molecular Biology 2019Quote: ... 200 µg lysate was mixed with 0.1 µl RNase A (~3 mg/ml) and increasing concentrations of RNasin (Promega), as indicated ...
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bioRxiv - Molecular Biology 2020Quote: ... and 0.5 ml (approximately 2 × 109 cells) were used for gDNA extraction with the Wizard Genomic DNA Purification kit (Promega), which typically yielded 50 μl of 0.5-1.5 μg/μl gDNA.
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bioRxiv - Microbiology 2020Quote: ... Protease was driven into the gel pieces by rehydrating them in 12 ng/ml trypsin in 0.01% ProteaseMAX Surfactant (Promega) for 5 minutes ...