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Citations for Promega :
951 - 1000 of 1604 citations for Phthalic Acid Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... the tprC ORF was amplified in a 100-μl final volume using 0.4 units of GoTaq polymerase (Promega) with approximately 10 ng of DNA template ...
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bioRxiv - Systems Biology 2022Quote: ... Dry pellets were resuspended in 100 μL of 50 mM TEAB and 2.5 μg of Trypsin/LysC (Promega) was added for overnight digestion at 37 °C ...
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bioRxiv - Microbiology 2020Quote: Mice infected with Antares- or Nluc-encoding viruses received 100 μL furimazine (NanoGlo™ furimazine, Promega, Madison, WI) diluted 1:40 in sterile endotoxin-free PBS (r.o. ...
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bioRxiv - Immunology 2020Quote: ... 200 µl/sample) and split into fractions with or without MNase (Promega, 50U/100 µl of nuclear lysate) and incubated for 1 hour at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were extracted using 100 μL of Passive Lysis Buffer from Dual-Luciferase reporter assay systems (Promega, E1980) according to manufacturer’s instruction and assayed using FLUOstar Omega (BMG Labtech) ...
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bioRxiv - Biochemistry 2020Quote: ... and added to 50-100 µL ribosome-coupled anti-Flag M2 agarose beads and 1 µL RNasin (Promega) per reaction ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was synthesized from 100-500 ng of total RNA using the GoScript reverse transcription reaction kits (Promega). Singleplex quantitative real-time polymerase chain reaction (qPCR ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were harvested and lysed with 100 μL of lysis buffer provided by a luciferase detection kit (Promega). After centrifugation ...
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bioRxiv - Microbiology 2019Quote: ... The cells were fixed with 4% paraformaldehyde for 5 min and permeabilised with 0.2 % Triton X-100 (Promega) in PBS for 10 min ...
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bioRxiv - Genetics 2020Quote: ... A 100 μL volume of collected medium was used for measurement by a GloMax Discover System GM300 (Promega). Signals were collected using a 520 nm excitation filter ...
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bioRxiv - Microbiology 2019Quote: ... we washed and lysed cells with 100 µL of 1X Passive Lysis Buffer (Dual Luciferase Assay Kit, Promega), freeze-thawed lysates ...
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bioRxiv - Plant Biology 2020Quote: ... LUC activity was measured with 20 μl of protein sample and 100 μl of LUC assay reagent (Promega). Activation from the DHS was calculated as (LUC luminescence/rate of MUG accumulation ...
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bioRxiv - Biochemistry 2020Quote: ... 100 µg of each sample was digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, WI, USA) at a ratio of 50:1 ...
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bioRxiv - Cancer Biology 2019Quote: ... ATP solution (5 μL of a 100 μM solution containing 0.1 μg 4:1 glycine:tyrosine peptide substrate (Promega)) was added and incubated at 37 °C for 20 min before the addition of ADP-Glo reagent (5μL ...
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bioRxiv - Bioengineering 2020Quote: ... PCR reactions were carried out with 100 ng genomic DNA or cDNA in the GoTaq Master Mix (Promega) according to the manufacturer’s instruction ...
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bioRxiv - Genetics 2021Quote: ... Substrate was added to a final concentration of 100 nM 11S fragment and 0.5X furimazine (Promega, NanoGlo Substrate). Luminescence was measured on a ViewLux HTS reader (PerkinElmer ...
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bioRxiv - Microbiology 2021Quote: ... Cells (100 μL) were then incubated with 4 μM cheopin or Fast Break lysis solution (Promega, Madison, WI) and absorbance at 405 nm was measured for 1h using a BioTek Synergy H1M plate reader set to 28°C.
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was generated from 20-100 ng of RNA using the GoTaq 2-step RT-qPCR System (Promega). qPCR was performed with SYBR Green on a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was generated from 20-100 ng of RNA using the GoTaq 2-step RT-qPCR System (Promega). qPCR was performed with SYBR Green on a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2021Quote: ... into 100 mM ammonium bicarbonate and digested overnight at 37 °C with mass spectrometry grade trypsin gold (Promega) at a 1:50 trypsin:protein ratio ...
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bioRxiv - Genomics 2020Quote: ... each of the wells containing 0.5 μl of lysis solution (0.2% Triton X-100 [Roche], 0.8 units of RNasin Plus [Promega], 4 mM dNTPs [Promega] and 1 μM of barcoded oligo-dT primers [E3V6NEXT ...
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bioRxiv - Cell Biology 2020Quote: ... The dried gel pieces were re-swollen by incubation in 100 mM ammonium bicarbonate pH 8.0 supplemented with trypsin (12.5 ng/μL; Promega) for 1 h at 4 C and then incubated overnight at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... and 100 μl of 50 mM ammonium bicarbonate buffer as well as 10 μl of protease solution (Promega Trypsin/Lys-C Mix ...
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bioRxiv - Microbiology 2023Quote: ... Each sample was mixed with 100 μl luciferase substrate (Bright-Glo Luciferase Assay System, purchased from Promega, USA), and luminescence was measured immediately by the GloMax Navigator System (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested in 100 mM ammonium bicarbonate containing 2 μg of trypsin/Lys-C enzyme mix (Promega) at 37 °C overnight ...
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bioRxiv - Immunology 2023Quote: ... The samples were then diluted with Tris-HCl (100 mM, pH 8.0) and digested with 0.5 µg sequencing-grade trypsin (Promega). The generated peptides were desalted and analyzed in duplicates by LC-MS/MS using a timsTOF fleX mass spectrometer equipped with a nanoElute LC system (Bruker) ...
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bioRxiv - Neuroscience 2023Quote: ... DA at concentrations ranging from 0.01 nM to 100 μM and 5 μM furimazine (NanoLuc Luciferase Assay, Promega) were then bath-applied to the cells ...
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bioRxiv - Plant Biology 2023Quote: ... Total proteome peptide pools were generated by sample digestion overnight with 1:100 sequencing grade trypsin (V5113; Promega). Generated peptide pools were quantified by Nanodrop ...
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bioRxiv - Plant Biology 2023Quote: The substrate of the nano-luciferase diluted at 1:100 (v/v) (furimazine, Kit from Promega (ref 1110)) was spread on seedling that grew on Agar plates ...
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bioRxiv - Microbiology 2023Quote: ... The size of any positive IS2404 detection was assessed against 2 µL of 100 bp DNA Ladder (Promega) and run at 50 V for 1.5 hrs before being visualised with an EZ Gel Documentation System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 µl of CellTiter-Glo® reagent from CellTiter-Glo® 3D cell viability assay kit (Promega-UK) was added to each well and mixed for 5 minutes on a plate shaker and incubated at room temperature (RT ...
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bioRxiv - Immunology 2023Quote: ... 100 mM ammonium bicarbonate was added followed by the addition of 0.5 µG/µL sequencing-grade trypsin (Promega) for protein digestion at 37°C for 18 h ...
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bioRxiv - Cell Biology 2024Quote: ... Solvent was removed in a vacuum centrifuge and 100 µl 10 ng/µl sequencing grade Trypsin (Promega, US) in 10 % ACN ...
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bioRxiv - Plant Biology 2024Quote: ... resuspended in 100 mM HEPES pH 7.5 and digested at 37°C overnight with Trypsin/Lys-C (Promega) at a protease-to-proteome ratio of 1:100 (wt/wt) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Bands were dehydrated and dried by speed vac before resuspension in 100 μL 0.2% ProteaseMAX™ surfactant (Promega) and 50mM NH4HCO3 solution containing trypsin (sequencing grade ...
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bioRxiv - Microbiology 2024Quote: ... Media was removed from each well prior to addition of 100 μL Bright-Glo™ luciferase substrate (Promega) diluted 1:20 in 1x lysis buffer ...
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bioRxiv - Neuroscience 2024Quote: ... cells were washed with HBSS and labeled with 100 nM HaloTag NanoBRET 618 ligand (Promega Corp., Fitchburg, WI) in phenol red-free Opti-MEM I reduced serum medium (Gibco/Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... The plates were brought to room temperature and 100 μL of room temperature CellTiter-Glo 3D (Promega G9683) were added to each of the wells ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were cleared by centrifugation and 200 µL of supernatant was incubated for 20 min at 21°C with 5 µg of RNA in 200 µL of 2xTENT buffer (100 mM Tris pH 7.8, 2 mM EDTA, 500 mM NaCl, 0.1% Tween) supplemented with RNAsin (Promega). RNA-protein complexes were then incubated with MyOne Streptavidin T1 DynaBeads (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... 50 to 100 ng of template were used per reaction with 1.5 µl rRNasin 40 U.ml-1 (Promega), 5 µl 5x optimized transcription buffer (Promega) ...
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bioRxiv - Genetics 2024Quote: ... 100 ng of genomic DNA was spiked with 0.5 ng of unmethylated lambda DNA (Catalogue Number: D1521, Promega) to assess the bisulfite conversion efficiency ...
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bioRxiv - Cancer Biology 2021Quote: ... the samples were diluted to 1 M Urea with 10mM EPPS pH 8.2 and incubated overnight with 1:50 LysC (Wako Chemicals) and 1:100 Sequencing grade trypsin (Promega). Digests were acidified using TFA and tryptic peptides were purified by tC18 SepPak (50 mg ...
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bioRxiv - Cell Biology 2020Quote: ... and immersed in 100 µL of growth medium and 20 µL of CellTiter 96 AQueousOne Solution Reagent (Promega Co.). After another incubation for 3 h at 37°C in a 5% CO2 atmosphere ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested with LysC (Wako) (substrate:enzyme=100) overnight at 37°C and then with sequencing grade trypsin (Promega) (substrate:enzyme=100 ...
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bioRxiv - Cell Biology 2020Quote: Proteins on beads were washed twice with 100 μL of 25 mM NH4HCO3 and we performed on-beads digestion with 0.2 μg of trypsine/LysC (Promega) for 1 h in 100 µL of 25 mM NH4HCO3 ...
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bioRxiv - Cell Biology 2020Quote: ... and harvested at varying timepoints by resuspending the cells in 50-100 μL of 1X Passive Lysis Buffer (Promega). Inductions were staggered so that all timepoints could be harvested simultaneously ...
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bioRxiv - Biochemistry 2022Quote: ... The gel pieces were then first dehydrated with 100 % acetonitrile and then rehydrated with sequence grade trypsin solution (0.6 µg, Promega) and incubated overnight at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... beads were resuspended in 100 µL of 25 mM NH4HCO3 and digested by adding 0.2 µg of trypsin/LysC (Promega) for 1 h at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: Gently re-suspend cells in 100 μl of 3% Glyoxal fixation solution with 1:25 RNasin Plus (Promega N261B) and incubate for 15 minutes on ice.
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bioRxiv - Physiology 2020Quote: ... cDNA was added as 100 ng/reaction to SYBR green based GoTaq® qPCR Master Mix (Promega, Wisconsin, USA). The primer sequence for CEBPa was F ...