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Citations for Promega :
1101 - 1150 of 1604 citations for Phthalic Acid Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: Total RNA extraction was performed with 100 mg of grounded GPR tissue using the ReliaPrep RNA Tissue to fibrous tissues (Promega). The RNA quality and purity were accessed with NanoDrop 2000 Spectrophotometer (Thermo Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Infected SupT1 cells were incubated for 48 h and lysed in 100 μL of Bright-Glo Luciferase Reagent (Promega, E2610). Infection was assessed by luciferase activity using a LUMIstar Omega microplate luminometer (BMG Labtech) ...
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bioRxiv - Microbiology 2020Quote: ... This was followed by in-gel digestion using 1:100 ratio enzyme:sample (w:w) using as enzyme sequencing grade modified trypsin (Promega, Madison, WI) overnight at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... the antibody–bead mixture was resuspended in 500 μl of the reaction mixture containing 10 μg of fragmented total RNA,100 μl of reaction buffer and 5 μl of RNasin Plus RNase Inhibitor (Promega), and incubated for at least 4 h at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: The medium in 96-well plate was replaced at the indicated time points with 100 μl of PBS supplemented with 0.2 g/l EDTA and 50 μM FITC-VAD-fmk (Promega, USA) or 50 μM 1,1’,3,3,3’,3’-hexamethylindodicarbo-cyanine iodide (DiIC1(5) ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293T cells were seeded in 24-well tissue culture plates at a density of 1.6 x 105 cells/well and transfected with 50-100 ng/well of pNL4-3-GFP using Fugene HD (Promega) transfection reagent ...
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bioRxiv - Cancer Biology 2021Quote: ... mix was used at a concentration of 100 μg/μl for overnight “on-bead” digestion using LysC-trypsin mix (Promega)2,3 ...
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bioRxiv - Cell Biology 2021Quote: ... 140 mM NaCl and 1% Triton X-100) and lysates used to assay luciferase activity in 96-well plates using the Dual-Luciferase Reporter Assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Trypsin digestion was carried out overnight at 37°C with 1:50-1:100 enzyme-protein ratio of sequencing grade-modified trypsin (Promega) in 50 mM NH4HCO3 pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... A total of 100 μg of protein was digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, WI, USA) using filter-aided sample preparation (FASP ...
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bioRxiv - Microbiology 2022Quote: ... was used to measure the luciferase activity of each well after the addition of 50-100 µl of reconstituted luciferase assay buffer (Promega) as per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was eluted from the beads using the elution buffer (1% SDS and 100 mM NaHCO3) and purified by PCR purification kit (Promega). The final products were analyzed by quantitative real-time PCR with the primers listed below:
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bioRxiv - Molecular Biology 2022Quote: ... 5 μg of the purified RNA was further treated in solution by 5 units of RQ1 DNase in 100 μl of 1x RQ1 buffer (Promega) for 1 h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... was used to measure the luciferase activity of each well after the addition of 50-100 µl of reconstituted luciferase assay buffer (Promega). Inhibitors were normalized to vehicle control.
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bioRxiv - Biochemistry 2022Quote: A large gel piece covering a wide MW range above 100 kDa was cut out and subjected to reduction/alkylation and in-gel digestion with trypsin (Promega) as previously described [19b] ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the proteins were alkylated with iodoacetamide (100 mM for 1 h at room temperature in a dark chamber) and digested with trypsin (Promega) diluted in ammonium bicarbonate (0.1 M for 24 h at 37 °C) ...
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bioRxiv - Cell Biology 2022Quote: ... and dried in at 37 °C before overnight digestion with 30 μL trypsin (1:100 g/g, sequencing grade modified trypsin V5111, Promega) at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... Nuclei pellets were re-suspended in 100 µl of PBS containing 1 U/μl RNasin® Ribonuclease Inhibitors (Promega, # N2615) with or without 5 µg/µl of yeast tRNA (Thermo ...
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bioRxiv - Cell Biology 2020Quote: ... and the proteins were on-bead digested using 5 µl of Sequencing Grade Trypsin (100 ng/µl in 10 mM HCl, Promega). The digestion was carried out at 37°C over night ...
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bioRxiv - Plant Biology 2022Quote: ... 100 ng total DNA was added to a 20 μl PCR containing 10 μl GoTaq® Master Mixes (Promega, USA) and 0.1 μM of each primer pair ...
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bioRxiv - Systems Biology 2019Quote: ... and dried in an oven at 37 °C) before overnight digestion with 20 μL trypsin (1:100 g/g, sequencing grade modified trypsin V5111, Promega) at 37 °C ...
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bioRxiv - Biophysics 2019Quote: ... cells were treated with the LRRK2 inhibitor MLi-2 (100 nM) for 2 hours and then labelled with the TMR ligand (3 μM)(Promega). After 15 minutes ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were harvested 36 h after transfection by lysing in 100 μl of 1× reporter lysis buffer (Promega, Madison, WI). Lysates were pelleted ...
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bioRxiv - Developmental Biology 2020Quote: ... the cells for the luciferase assay were exposed to 100 μL of a 1:1 dilution of DMEM and luciferase substrate (ONE-Glo Luciferase Assay System, Promega), and luminescence from each well measured in a GloMax-96 Microplate Luminometer (Promega) ...
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bioRxiv - Microbiology 2021Quote: PS cells were seeded at 1.5 × 105 cells per well in 96-well plates (n=3) and transfected with in vitro transcribed TBEV replicon RNA (100 ng/well) and 10 ng of pTK-Ren (Promega) using Lipofectamine-2000 (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... The cytotoxicity of compounds (100 uM to 1 uM, 3-fold dilution) was examined using the CellTiter-Glo Luminescent Cell Viability Assay (Promega). Cell cytotoxicity data was normalized to DMSO control as 0% cell death.
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bioRxiv - Microbiology 2020Quote: ... Reduced and alkylated proteins from band slices excised from the entire separation profile were in-gel digested at 37°C overnight with 100 ng trypsin (modified sequencing grade, Promega). Supernatants were dried in a vacuum centrifuge and resuspended in 0.1% TFA ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were then transiently-transfected with 100 ng/well cDNA encoding NLuc/NRP1 or NLuc/VEGFR2 using FuGENE (Promega, USA) according to the manufacturer’s instructions and incubated for a further 24h at 37°C/5% CO2 ...
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bioRxiv - Molecular Biology 2020Quote: ... 200 ng of DNase treated RNA library was added to 100 nM of Halo-tagged proteins immobilized onto magnetic resin (Promega). The volume of each binding reaction was 100µl in SEQRS buffer containing 200 ng yeast tRNA competitor and 0.1 units of RNase inhibitor (Promega) ...
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bioRxiv - Microbiology 2021Quote: ... resuspended in 50 μL (fLuc assays) or 100 μL (NanoLuc assays) of 1× Cell Culture Lysis Reagent (Luciferase Assay System; Promega) and incubated for 10 min at room temperature ...
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bioRxiv - Immunology 2021Quote: We seeded 1 × 105 of HEK293 cells into 24-well plates in triplicate and transfected the cells with an expression vector together with p-125 Luc reporter (100 ng/well) and phRL-TK (10 ng/well, Promega) plasmids ...
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bioRxiv - Microbiology 2019Quote: ... Pelleted cells suspended gently in 100 μl of TEAB were mixed with 50 ng/µL of sequencing grade modified porcine trypsin (Promega), and digestions were incubated at 37 °C for 15 min ...
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bioRxiv - Biochemistry 2019Quote: ... aliquot of approximately 107 cells were resuspended in 100 μL of lysis buffer (0.1 % w/v ProteaseMAX Surfactant (Promega, USA) in 50 mM ammonium bicarbonate and 10 % v/v ACN) ...
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bioRxiv - Genetics 2020Quote: ... The firefly luminescence from each of the constructs was measured after incubating 20 µL of each reaction with 100 µL of Dual-Glo Luciferase reagent (Promega) for 10 min ...
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bioRxiv - Immunology 2020Quote: ... was synthesised in 20 µl reaction volumes with 500 ng (ISGs) or 100 ng (PIV5-infected cells) total RNA and oligo(dT) using GoScript reverse transcriptase (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Linear replicon DNA (500 ng) was added to transcription reactions at a final volume of 100 μl containing the following: Transcription Optimised Buffer (Promega), 10 mM DTT (Promega) ...
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bioRxiv - Biochemistry 2021Quote: ... and 20 mM iodoacetamide respectively and washed again twice with 200 μl of 100 mM aqueous TEAB before incubating with 2 μg of trypsin (proteomics grade, Promega) at 37°C for 4h ...
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bioRxiv - Molecular Biology 2021Quote: ... Firefly luciferase activity was measured at 550 to 570 nm wavelength and followed by 100 μl of 1X Stop & Glo Reagent (part of the kit E1910, Promega) was added to each well ...
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bioRxiv - Cell Biology 2020Quote: ... using one of the following antibodies against (all dilutions in PBS): HaloTag® polyclonal anti-rabbit (G928A, Promega, 1:100); ACTN2 monoclonal anti-mouse (EA-53 Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: Urinary proteins (100 μg of each sample) were digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, WI, USA) using filter-aided sample preparation (FASP ...
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Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... Trypsin digestion was carried out overnight at 37 °C with 1:50 - 1:100 enzyme-protein ratio of sequencing grade-modified trypsin (Promega) in 50 mM NH4HCO3 pH 7.5 ...
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bioRxiv - Immunology 2021Quote: ... the plates were washed with PBS and the cells were lysed with 1× reporter lysis buffer (100 µl per well; Promega). Following a freeze-thaw cycle ...
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bioRxiv - Cell Biology 2021Quote: ... Aliquots of culture medium (300 μl) were taken at indicated timepoints and mixed with 100 μL of 4X RealTime-Glo extracellular ATP assay reagent (Promega) reconstituted in RPMI 1640/B27(-Ins ...
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bioRxiv - Neuroscience 2021Quote: ... The transfected cells were then treated with Aβ42 (1-4 μM) or calcitriol (100 nM) for 6 h before luciferase activity assay (Dual-Luciferase Reporter Assay, Promega). A 587-bp region of the human Cyp24a1 gene promoter that contains two VDRE motifs was cloned into the promoter-less luciferase expression vector pGL3-basic (Promega ...
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bioRxiv - Biochemistry 2021Quote: ... of 50mM Tris-HCl pH 8.5 containing LysC (Wako Chemicals) at 1:100 (w/w) ratio and trypsin (Promega V5113) at 1:100 (w/w ...
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bioRxiv - Immunology 2022Quote: 1×105 721.221 or 293T cells were plated in 24 well plates and transfected with 400 ng of pGL3 and 100 ng of pRL-CMV (Promega). Two days later ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were on-bead digested using 5 μl of Sequencing Grade Trypsin (100 ng/μl in 10 mM HCl, Promega). The digestion was carried out in a microwave instrument (Discover System ...
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bioRxiv - Cell Biology 2022Quote: ... The slurry was then diluted four times with 20mM Tris-HCl with 2mM CaCl2 before digestion overnight with 100 ng sequencing grade trypsin (Promega). The samples were then centrifuged for peptide extraction from the supernatant which were then subject to liquid chromatography LC-MS (PROXEON coupled to a QExactive mass spectrometer ...
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bioRxiv - Molecular Biology 2022Quote: ... cells transduced with the indicated sgRNAs were seeded in a 96-well plate in 100 µL of media and 50 µl of Cell Titer Glo reagent (Promega) was added to each well and the luminescence read on the SpectraMax M5 plate reader (Molecular Devices).
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bioRxiv - Evolutionary Biology 2022Quote: ... Flies were placed individually in 100 ul Chelex solution (5g of Chelex mixed with 95ml TE buffer) with 5 ul proteinase K (Promega), and incubated overnight at 56°C ...