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Citations for Promega :
51 - 100 of 1059 citations for Two Hybrid cDNA Library since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Transient transfection of A36-p14 hybrid constructs and the pE/L-LifeAct-iRFP670 expression in Vaccinia-infected cells was done using FUGENE (Promega) as described by the manufacturer ...
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bioRxiv - Physiology 2021Quote: ... purified total RNA was synthesized into cDNA:RNA hybrids with Maxima H Minus Reverse Transcriptase (Thermo) and primed using equal amounts of oligo(dT)15 primers (Promega) and random hexamers (Thermo) ...
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bioRxiv - Molecular Biology 2022Quote: ... The firefly luciferase activity was detected in homogenates of organs by luminescence measurements using Synergy H4 hybrid reader (Bio-Tek) and Bright-Glo Luciferase Kit (Promega).
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bioRxiv - Neuroscience 2020Quote: ... Two days exposure to alkaline phosphatase (AP) substrate NBT/BCIP (Promega, S3771) was necessary to detect AP-DIG-labeled Vax1 mRNA at maximal level ...
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Interdependent Iron and Phosphorus Availability Controls Photosynthesis Through Retrograde SignalingbioRxiv - Plant Biology 2021Quote: ... Two μg of the total RNA was used for reverse transcription (Promega) to synthesize cDNA using oligo(dT ...
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bioRxiv - Bioengineering 2020Quote: Two different variations of the CellTiter-Glo® 3D Viability assay (Promega) were used in this study ...
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bioRxiv - Genomics 2020Quote: ... The solution was syringed-filtered through two Wizard Genomic DNA columns (Promega) and then washed three times with 80% isopropanol ...
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bioRxiv - Genetics 2022Quote: ... After treatment of two µg RNA with RQ1 RNase-free DNase (Promega) and reverse transcription using M-MLV Reverse Transcriptase (Promega ...
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bioRxiv - Microbiology 2022Quote: ... Two μg RNA was subsequently treated with 2U RNA-free DNAse (Promega) following the manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... the ratio of the two luciferases was determined (Dual luciferase kit, Promega). For generation of inducible BJAB cells ...
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bioRxiv - Synthetic Biology 2024Quote: ... a Firefly Luciferase with two destabilization sequences has been used (Luc2CP, Promega).
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bioRxiv - Synthetic Biology 2024Quote: ... a Firefly Luciferase with two destabilization sequences has been used (Luc2CP, Promega), while for the biosensing experiments ...
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bioRxiv - Immunology 2022Quote: The constant regions of the human TCR genes were replaced with murine constant genes by overlapping PCRs as previously described,14 and the human/murine hybrid TCR genes were sub-cloned into the pGEM-4Z vector (Promega Corporation) for mRNA expression ...
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bioRxiv - Genetics 2023Quote: ... After adding 2 µg MS grade trypsin in the intraspecific hybrids (Pierce Biotechnology, Waltham, MA) and polyploids (Promega Corporation, Madison, WI) to each sample ...
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bioRxiv - Immunology 2020Quote: ... and cDNA synthesized from 1 µg total RNA (High Capacity cDNA Reverse Transcription Kit, Promega). Quantitative RT-PCR was performed using FluoCycle II SYBR Master Mix (EuroClone ...
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bioRxiv - Plant Biology 2019Quote: ... Two micrograms of total RNA were treated with RQ1 RNase-Free DNase (Promega) and processed with the ImProm-II Reverse Transcription System (Promega ...
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bioRxiv - Plant Biology 2023Quote: Two vials of trypsin/Lys-C mix (100µg, V5078, Promega, Alexandria, NSW, Australia) were dissolved into 1 mL of the resuspension buffer (50mM acetic acid ...
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bioRxiv - Molecular Biology 2023Quote: ... The assay was validated using Taq DNA polymerase from two different manufacturers (Promega Gotaq Flexi DNA polymerase or Invitrogen Taq DNA polymerase ...
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bioRxiv - Pathology 2023Quote: ... we performed two overlapping PCR on the RCA products with GoTaq Flexi (Promega) according to the manufacturer’s protocol to amplify the complete genome of MSV using primers targeting MSV-G (PCR#1 ...
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bioRxiv - Systems Biology 2021Quote: ... a human cDNA pool (Promega Corporation), or obtained as synthesized double-stranded DNA fragments (gBlocks ...
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bioRxiv - Biochemistry 2023Quote: ... LgBiT and Halo-Ub cDNA (Promega) were transfected using FuGENE HD (Promega ...
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bioRxiv - Developmental Biology 2020Quote: ... and cDNA was synthesized by reverse transcription using the First-Strand cDNA Synthesis Kit (Promega, Madison, WI). qPCR reactions were performed using Bio-Rad SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNA synthesis was performed on 1 µg of RNA using ProtoScript First Strand cDNA Synthesis Kit (Promega). Primers recognizing exons flanking the CRISPR-targeted exon (Supp ...
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bioRxiv - Genomics 2021Quote: ... Purified RNA was reversed transcribed to cDNA using Maxima H Minus first Strand cDNA synthesis kit (Promega). The sequences of the primers used are available in S6 Table ...
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bioRxiv - Microbiology 2020Quote: ... Two-rounds of translocation PCR was performed using GoTaq G2 Green Master Mix (Promega). In the first round PCR ...
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bioRxiv - Systems Biology 2020Quote: ... There were two positive controls: one was pGL4.10 vector (Promega, with luc2 firefly gene) with pTran promoter inserted between NheI and XhoI sites ...
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bioRxiv - Microbiology 2022Quote: ... Two μg of total RNA was treated with RQ1 DNase enzyme (Promega; Madison, WI) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Two days after transfection with 2 to 2.5 μg DNA using Fugene HD (Promega), confocal images were acquired using an FV1000 confocal laser scanning microscope (Olympus ...
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bioRxiv - Synthetic Biology 2020Quote: ... Proteins were digested with two separate and sequential aliquots of sequencing grade trypsin (Promega) of 1 μg ...
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bioRxiv - Bioengineering 2024Quote: ... two cytocompatibility tests were performed using cell proliferation MTS assay (Promega, Cat. No. G3580). This assay involved the conversion of MTS [3-(4,5-dimethyl-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] into formazan by living cells in a reducing environment ...
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bioRxiv - Cell Biology 2023Quote: ... Libraries were amplified using GoTaq (Promega), with 20 cycles for PCR1 and 15 cycles for nested PCR2 on 50% material from 1st PCR ...
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bioRxiv - Cancer Biology 2021Quote: ... total RNA (2 µg) was reverse transcribed into cDNA using a cDNA synthesis kit (Promega, Madison, WI, USA). The RT-PCR was assessed using r-Taq plus Master Mix (Elpis Biotech ...
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... cDNA was diluted at 1/80 and qPCR were performed using 1 μl of diluted cDNA (GoTaq Promega) and run on QuantStudio 3 Real Time PCR system ...
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bioRxiv - Pathology 2021Quote: ... The nanoluciferase (NL) cDNA was from Promega, with an N-terminal fusion of the IL-6 signal sequence (Hall et al. ...
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bioRxiv - Plant Biology 2022Quote: ... were used to synthesize cDNA (Promega, #1012891). RT-qPCR (Real-Time quantitative PCR ...
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bioRxiv - Plant Biology 2019Quote: ... before cDNA synthesis using Reverse Transcriptase (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Human LAMA1 cDNA was purchased from Promega and sub-cloned into pLVX-Tet-On Advanced vector (Clontech) ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was prepared using random hexamers (Promega) and the ImProm-II Reverse Transcription System (Promega) ...
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bioRxiv - Neuroscience 2022Quote: ... and cDNA was generated using GOSCRIPT (Promega) reverse transcriptase according to the manufacture’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was obtained using random hexamers (Promega) and Revert Aid reverse transcriptase (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... After two days incubation at 37°C cell viability was assessed using CellTiterGlo (Promega, G7570) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... lanes were split in two pieces and digested with sequencing-grade modified trypsin (Promega, #V5111) and alpha-lytic protease (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... Seventy-two hrs after electroporation cell viability was determined by measuring ATP levels (Promega G9241) and 30 ul of cell supernatant was transferred to fresh SUP-T1 cells (105 cells in 500 ul) ...
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bioRxiv - Developmental Biology 2022Quote: ... LUC activity was evaluated two days after agroinfiltration in leaves sprayed with D-Luciferin (Promega) using the NEWTON 7.0 CCD imaging system (Vilber) ...
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bioRxiv - Biochemistry 2022Quote: ... Two identical 50 μL reactions were prepared consisting of 25 μL GoTaq DNA Polymerase (Promega), 0.5 μL forward primer ...
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bioRxiv - Cancer Biology 2023Quote: ... at one week and at two weeks and measured by CellTiter-Glo (Promega #PR-G7573) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... Libraries were cleaned using ProNex beads (Promega) with a 1.5:1 ratio of beads to sample ...
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bioRxiv - Microbiology 2022Quote: Final libraries were quantified by QuantiFluor (Promega) in 96 well plates ...
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bioRxiv - Molecular Biology 2020Quote: ... then 1 µg RNA was used to synthesize the oligo(dT) primed first-strand cDNA using the M-MLV reverse transcriptase cDNA synthesis kit (Promega). Quantitative RT-PCR was performed using a CFX96 Real-Time PCR detection system (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was then synthesized using 2 μg of mRNA and a Transcriptase first-strand cDNA synthesis kit (Promega, Madison, WI). Real-Time qPCR to detect the indicated targets was performed on a LightCycler® 480 System (Roche Life Science ...