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Citations for Promega :
401 - 450 of 1059 citations for Two Hybrid cDNA Library since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... First-strand cDNA synthesis was performed using M-MLV Reverse Transcriptase (Promega) and specific primer of L1 gene ...
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bioRxiv - Microbiology 2024Quote: ... Complementary DNA (cDNA) synthesis was performed using GoScript™ Reverse Transcriptase (Promega) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... The cDNA was then purified with ProNex magnetic beads (Promega, WI, USA) according to the Iso-Seq Express Template Preparation for Sequel and Sequel II Systems protocol (Pacific Biosciences ...
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bioRxiv - Plant Biology 2023Quote: ... first-strand cDNA was synthesized using the ImProm-II Reverse Transcriptase (Promega). Three cDNA samples derived from three independent RNA extractions were synthesized and amplified using Luna®Universal qPCR Master Mix (New England Biolabs ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were harvested two days post-transfection and lysed using the Passive Lysis Buffer provided in the Dual-Luciferase Reporter Assay System (Promega, Madison, WI). The relative activities of the firefly luciferase and renilla luciferase domains in the clarified lysate were then measured using the Dual-Luciferase Reporter Assay System on a Synergy Neo2 plate reader (Biotek ...
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bioRxiv - Microbiology 2023Quote: ... Two aliquots of 100 µL were transferred into new tubes and digested either with a sequencing-grade trypsin (Promega, Madison, WI, USA) or chymotrypsin (Promega ...
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bioRxiv - Neuroscience 2023Quote: Intracellular ATP concentrations were measured in 38-45 independent wells per group from two unique culture preparations using the CellTiter-Glo 2.0 reagent (Promega Corp.; Madison, WI) as described (21) ...
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bioRxiv - Genomics 2021Quote: ... The prepared sequencing libraries were quantified using a GloMax Plate Reader (Promega) and equal-mass pooled into 96-sample pools ...
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bioRxiv - Physiology 2021Quote: ... The double stranded library is quantified using QuantiFluor ONE dsDNA System (Promega) and equal 330 ng in a total volume of 60 ml or less ...
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bioRxiv - Cancer Biology 2021Quote: ... The double stranded library is quantified using QauntiFluor ONE dsDNA System (Promega). The library is circularized at 37 °C for 30 min ...
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bioRxiv - Microbiology 2022Quote: ... The resulting purified libraries were finally quantified using a Quantus fluorometer (Promega) and combined in equimolar amounts prior to paired-end sequencing (2 x 250-bp ...
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bioRxiv - Cell Biology 2023Quote: ... The double stranded library is quantified using QauntiFluor ONE dsDNA System (Promega). The library is circularized at 37 °C for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Concentration of ChIP-nexus libraries was measured using the Quantus fluorometer (Promega) and fragment size distribution was checked on Agilent Bioanalyzer using the High Sensitivity DNA Assay ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNA was generated from the isolated RNA using a GoScript™ Reverse_Transcription_System (Promega) according to the vendor’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The amount of synthesised cDNA was determined using the QuantiFluor ssDNA system (Promega) with a Qubit® 2.0 Fluorometer (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by reverse transcription to generate cDNA with GoScript Reverse Transcription Kit (Promega). Quantitative PCR was performed by using the Agilent Mx3000P qPCR system with the 2×SYBR Green qPCR Master Mix (Bimake) ...
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bioRxiv - Molecular Biology 2021Quote: ... RAI1 and TCF20 cDNA sequences were obtained from Promega (products FXC01995, FHC01638, FXC11595). RAI1 ePHD domain was codon-optimized for bacterial expression ...
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bioRxiv - Microbiology 2019Quote: ... Quantification of the cDNA was carried out using the QuantiFluor ssDNA system (Promega) with the Qubit Fluorometer (Life Technologies).
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bioRxiv - Plant Biology 2019Quote: ... cDNA was synthesized from 1µg plant RNA using M-MLV reverse transcriptase (Promega) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... First strand cDNA was generated using the Reverse Transcription System (Promega, Madison, WI) according to the manufacturer’s instruction ...
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bioRxiv - Physiology 2019Quote: ... and then converted to cDNA using MMLV Reverse Transcriptase with random primers (Promega). Quantitative RT-PCR was carried out with either TaqMan™ Universal PCR Master Mix or SYBR Green PCR master mix on the QuantStudio 7 Flex Real time PCR system (Applied Biosystems) ...
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bioRxiv - Evolutionary Biology 2020Quote: The RNA was transcribed to cDNA using M-MLV reverse transcription kit (Promega) following the manufacturer’s recommendations using a defined amount of RNA (1μg for bees and 50 ng for mites ...
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bioRxiv - Cell Biology 2021Quote: ... Kif9 and B-actin cDNAs were amplified by GoTag Green Master Mix (Promega) and with the following primers:
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bioRxiv - Cell Biology 2021Quote: ... Splicing was analyzed by radiolabeled PCR of resulting cDNA using GoTaq Green (Promega) spiked with α-32P-deoxycytidine triphosphate (dCTP) ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was generated using oligo(dT)20 primer (GoScript Reverse Transcription System, Promega), before RT-qPCR using primers for socs3 and gapdh ...
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bioRxiv - Microbiology 2021Quote: ... cDNA of 1.5 µg RNA was produced using M-MLV reverse transcriptase (Promega) and Oligo(dT)15 primers (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... RNA (3μg) was used to prepare cDNA using reverse transcriptase (Promega, United States) and oligodT15 ...
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bioRxiv - Cancer Biology 2020Quote: ... Synthesis of cDNA was performed following standard protocols using MMLV Reverse Transcriptase (Promega). Quantitative RT-PCR was performed using SYBR Green (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: RNA was first reverse-transcribed to cDNA using GoScript Reverse Transcriptase kit (Promega) following the manufacturers protocol ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was synthesized from 10 μg total RNA using AMV Reverse Transcriptase (Promega) and oligo-dT ...
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bioRxiv - Neuroscience 2021Quote: ... BF OT cDNA fragments were inserted into the pGEM-T easy vectors (Promega). The plasmids were digested with restriction enzymes (NcoI or SpeI ...
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bioRxiv - Neuroscience 2021Quote: ... The cDNA encoding the full-length mouse HCN2 was cloned in pCI (Promega) mammalian expression vector ...
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bioRxiv - Microbiology 2021Quote: cDNAs from RNA isolated sample was obtained using M-MLV reverse transcriptase (Promega) following the manufacturer instructions ...
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bioRxiv - Microbiology 2019Quote: ... cDNA synthesis was carried out using the GoScript™ Reverse Transcription kit (Promega). cDNA was diluted 1:30 into molecular biology grade water and used as template in qRT-PCR reactions using SYBR Green (Applied Biosystems™ ...
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bioRxiv - Microbiology 2019Quote: ... cDNA was synthetized from 1µg RNA using the Reverse Transcription system kit (Promega). The qPCR reactions were performed with PowerUp™ SYBR™ Green Master Mix (Thermofisher ...
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bioRxiv - Neuroscience 2019Quote: ... cDNA fragments were ligated to pGEM T Easy vector (Promega, Madison, WI, USA) and used to transform NEB 5-α competent Escherichia coli cells (New England Biolabs ...
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bioRxiv - Immunology 2020Quote: ... and cDNAs were synthesized using M-MLV reverse-transcriptase (Promega, Madison, WI, USA) using random hexamer nucleotides as primers (Promega) ...
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bioRxiv - Systems Biology 2020Quote: ... First-strand cDNA was synthesized by M-MLV reverse transcriptase (Promega, car# M170B) and diluted by a factor of 10 for quantitative PCR ...
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bioRxiv - Cancer Biology 2019Quote: ... cDNA was yielded from purified mRNA using ImProm II Reverse Transcription kit (Promega). Quantitative-RT PCR was then performed using the SensiFAST SYBR Green Hi-ROX master mix (Bioline) ...
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bioRxiv - Microbiology 2020Quote: ... cDNA synthesis was carried out using the GoScript™ Reverse Transcription kit (Promega). cDNA was diluted 1:30 into molecular biology grade water and amplification was quantified using SYBR Green (Applied Biosystems™) ...
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bioRxiv - Immunology 2019Quote: ... cDNA was generated using Moloney murine leukemia virus (M-MLV) reverse transcriptase (Promega) with random hexamer primers ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was generated from 500ng isolated RNA using M-MLV reverse transcriptase (Promega) and diluted 1:5 for use in 12µL qPCR reactions using SYBR® Green PCR master mix (Applied Bioscience ...
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bioRxiv - Physiology 2020Quote: ... with cDNA produced using the ImProm-II RT system (Promega, Madison, WI, USA). Real-time quantitative PCR was performed using a Prism 7000 (Applied Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... Splicing was analyzed by radiolabeled PCR of resulting cDNA using GoTaq Green (Promega) supplemented with α-32P-deoxycytidine triphosphate (dCTP) ...
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bioRxiv - Biochemistry 2021Quote: ... Complete cDNA was generated from total RNA using GoScript Reverse Transcription system (Promega), using 1 μg RNA per reaction and 0.5 μg of Random primer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Probes were synthesized from cDNAs cloned into the pGEM®-T Easy (Promega) plasmid ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA synthesis was carried out with M-MLV enzyme (Promega, Madison WI, USA) for 50 min at 37°C followed by 10 min at 70°C using random oligomers (hexamers ...
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bioRxiv - Cell Biology 2020Quote: ... One microgram of total RNA was reverse transcribed to generate cDNA (A3500, Promega). cDNA was amplified via fluorescently labeled Taqman primer sets using an Applied Biosystems 7900HT Fast Real Time PCR device ...
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bioRxiv - Biophysics 2020Quote: ... cDNAs were amplified and PCR fragments were subcloned into pGEM-HA vector (Promega) for expression in Xenopus oocytes ...
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bioRxiv - Evolutionary Biology 2021Quote: ... cDNA synthesis was performed with 0.5 µg of RNA using random primers (Promega) and the MMLV reverse transcriptase enzyme (Promega ...