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Citations for Promega :
1 - 50 of 1059 citations for Two Hybrid cDNA Library since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: Mammalian two-hybrid assay was performed using CheckMate™ Mammalian Two-Hybrid System (Promega, CAT# E2440) following the protocol supplied by the manufacturer ...
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bioRxiv - Biochemistry 2024Quote: Mammalian two-hybrid assays were performed following the instruction of the CheckMate™ Mammalian Two-Hybrid System (Promega). Constructs were synthesized by Genscript (Fig ...
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bioRxiv - Bioengineering 2019Quote: The two-hybrid system used in this study was developed from commercial CheckMate™ Mammalian Two-Hybrid System (Promega, Madison, WI, USA) with some modifications ...
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bioRxiv - Immunology 2022Quote: Based on Checkmate mammalian two-hybrid system (Promega, Madison, WI, USA), the indicated genes were engineered into pACT and pBIND vectors ...
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bioRxiv - Cancer Biology 2022Quote: Assays were performed using CheckMate Mammalian Two-Hybrid System (Promega E2440). The sequence encoding MED1 (amino acids 495-699) ...
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bioRxiv - Cell Biology 2020Quote: ... and SERBP1 were amplified from human cDNA and cloned into the appropriate vectors (pACT or pBIND) provided by the CheckMate Mammalian Two-Hybrid kit (Promega E2440). The appropriate combination of edited pACT and pBIND vectors ...
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bioRxiv - Microbiology 2020Quote: The interactions between host protein and RHDV nonstructural proteins were evaluated using a CheckMate Mammalian Two-Hybrid System (Promega). The proteins expressed from the pACT vector recombinant plasmid acted as prey proteins ...
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bioRxiv - Genetics 2020Quote: ... WT G4 mESCs (129/Sv × C57BL/6 F1 hybrid background)54 were co-transfected with the two respective sgRNA-pX459 vectors using the FUGENE HD transfection reagent (Promega) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Two (2) μg of total RNA were used for cDNA synthesis with MMLV (Promega) and random hexamers (Invitrogen) ...
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bioRxiv - Plant Biology 2021Quote: ... and used for cDNA library preparation with GoScript reverse transcriptase (Promega), using oligo(dT ...
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bioRxiv - Microbiology 2022Quote: ... cDNA library was built by M-MLV Reverse transcriptase (Promega, USA) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Two micrograms of total RNA were used for cDNA synthesis with a reverse transcription kit (Promega). Quantitative real-time PCR was performed using the SYBR premix kit (Promega) ...
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bioRxiv - Systems Biology 2019Quote: ... Half of the cDNA library was PCR amplified using Pfu polymerase (Promega, M7745) using the P5 forward PCR oligonucleotide and barcoded BC reverse oligonucleotides (200 nM ...
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bioRxiv - Bioengineering 2023Quote: ... Small RNA cDNA libraries were generated with M-MLV Reverse Transcriptase (Promega, M1705) before applying to the Illumina HiSeq2500 (Illumina ...
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bioRxiv - Microbiology 2022Quote: Two micrograms of DNAse-free RNA was used for cDNA synthesis using an InProm-II Reverse transcription system (Promega) according to the manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA libraries were generated using M-MLV RT [H-] Point mutant (Promega, WI, USA) according to the manufacturer’s directions ...
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bioRxiv - Neuroscience 2020Quote: ... Accurate quantitation of cDNA libraries was performed by using the QuantiFluor™ dsDNA System (Promega, USA). The size range of final cDNA libraries was determined applying the DNA chip on the fragment analyzer (average 350 bp ...
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bioRxiv - Neuroscience 2020Quote: ... Accurate quantitation of cDNA libraries was performed by using the QuantiFluor™ dsDNA System (Promega, U.S.A.). The size range of final cDNA libraries was determined applying the DNA chip on the fragment analyzer (average 350 bp ...
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bioRxiv - Neuroscience 2023Quote: ... The cDNA library was subjected to quality control by a QuantiFluor dsDNA system (E2671, Promega, WI) and an Agilent D5000 ScreenTape system before being sent to scRNA-seq (NovaSeq 6000 ...
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Differences in orthodenticle expression promote ommatidial size variation between Drosophila speciesbioRxiv - Evolutionary Biology 2021Quote: ... Accurate quantitation of cDNA libraries was performed using the QuantiFluor™dsDNA System (Promega, Madison, Wisconsin, USA). The size range of final cDNA libraries was determined by applying the DNA 1000 chip on the Bioanalyzer 2100 from Agilent (280 bp) ...
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bioRxiv - Plant Biology 2021Quote: The cDNAs corresponding to the two alleles of SoIAA2 were cloned into a pFN2A (GST) flexi vector (Promega – primers in Supplementary Table 1), expressed in E ...
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bioRxiv - Pathology 2020Quote: ... The partial sequence encoding the nucleoprotein from HPWMoV was amplified using specific primers (Table 1) from two-fold diluted cDNA with GoTaq® Flexi DNA polymerase (Promega, WI, USA) with the following reaction conditions ...
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bioRxiv - Microbiology 2019Quote: ... Quality control and quantification of the cDNA and Sequence Library was performed using Quantifluor dsDNA System (Promega, UK). Sequence libraries were run on a Miseq using MiSeq V2 300 cycle kit (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... Quality control and quantification of the cDNA and Sequence Library was performed using Quantifluor dsDNA System (Promega, UK). Sequence libraries were run on a Miseq using MiSeq V2 300 cycle kit (Illumina ...
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bioRxiv - Cancer Biology 2022Quote: ... After two rounds of trypsin (Promega) digestion ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: Group two-choice assay: agarose (Promega) substrates were prepared as follows ...
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bioRxiv - Cell Biology 2022Quote: ... After cloning prkar1aa from a zebrafish retina cDNA library and inserting into pGemT-Easy vector (#A1360) from Promega (Madison, WI), site-directed mutagenesis was performed as described in Liu & Naismith (32 ...
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bioRxiv - Cell Biology 2021Quote: ... following the manufacturer’s protocols and 250-500 ng of RNA was used to generate a cDNA library with MMLV H-Reverse transcriptase (Promega) and Oligo dT15 primers (Promega ...
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bioRxiv - Neuroscience 2021Quote: The Cacnb2 3’UTR (Ensemble ID: ENSG00000165995) was amplified from a rat brain cDNA library and cloned into the pmirGLO dual-luciferase expression vector (Promega) using the PfuPlus! DNA Polymerase (Roboklon) ...
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bioRxiv - Developmental Biology 2024Quote: ... ORFs for fam83fa and fam83fb were amplified from cDNA libraries and either TA cloned using the pGEM-T Easy Vector System (Promega) or the pENTR/D-TOPO vector system (Thermo Fisher) ...
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bioRxiv - Immunology 2022Quote: Fragments of each gene of interest obtained by amplification of cDNA libraries using specific primers were inserted into pGEM-T Easy vectors (Promega, USA) and transformed into competent E ...
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bioRxiv - Cell Biology 2023Quote: ... we generated pGEMT-col1a2 by cloning the zebrafish col1a2 sequence from a self-made zebrafish cDNA library into the pGEMT-easy vector using pGEM®-T Easy Vector Systems (Promega). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... pre-amplified cDNA libraries were diluted with addition of 9µL water to each well before quantification using QuantiFluor® dsDNA System (Promega, #E2671) and normalisation and dilution to 100pg ul-1.
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bioRxiv - Microbiology 2019Quote: ... Two hundred μL of protein precipitate solution (Promega) was added ...
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bioRxiv - Biophysics 2022Quote: ... the two purified flanking PCR fragments (Promega, USA) were assembled with BamHI and HindIII-digested PK18 (Gmr ...
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bioRxiv - Microbiology 2020Quote: ... for gram-negative bacteria and cDNA library preparation was performed using the GoTaq® 2-Step RT-qPCR System (A6010, Promega, USA). The RT-PCR was done using the Applied Biosystems™ 7500 Real-Time PCR System and primers shown in the supplementary data table S2 (35-38) ...
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bioRxiv - Microbiology 2020Quote: ... The resulting cDNA libraries were verified using the MultiNA System (Shimadzu, Kyoto, Japan) and quantified using a Quantus Fluorometer (Promega, Madison, WI, USA). Indexed libraries were then converted and sequenced (150-bp paired-end reads ...
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bioRxiv - Cancer Biology 2020Quote: ... RT-PCRs were conducted in two-step reactions by using two-step RT-PCR: M-MLV reverse transcriptase in M-MLV RT-PCR system (Promega, Madison, WI) and Platinum Taq polymerase (GeneMark ...
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bioRxiv - Biophysics 2022Quote: ... Two PCR reactions using GoTaq G2 PCR mix (Promega) and a pUC19 template were set up ...
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bioRxiv - Evolutionary Biology 2021Quote: ... we extracted DNA from parents and hybrid fish using the Wizard SV Genomic DNA Purification kit (Promega), simultaneously digested samples with SphI and MluCI restriction enzymes (New England Biolabs) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Locusta cDNA was synthesized using the cDNA Synthesis Kit (Promega). 2.5 ng of cDNA was used in each qPCR reaction ...
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bioRxiv - Evolutionary Biology 2024Quote: ... cDNAs were synthesized using the GoScript cDNA synthesis procedure (Promega) prior to concentration assessment using the Qubit High sensitivity DNA kit (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... Contaminating genomic DNA was depleted by two DNase treatments (Promega), according to manufacturer’s recommendations ...
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Kinetics Of Interferon-λ And Receptor Expression In Response To In Vitro Respiratory Viral InfectionbioRxiv - Immunology 2021Quote: Two micrograms of total RNA were treated by DNase (Promega) and then directly reverse transcribed using oligo-dT16 primers and MMLV reverse transcriptase (Promega) ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were digested in two steps with LysC/trypsin (Promega). After overnight trypsin digestion in 2 M Urea ...
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bioRxiv - Plant Biology 2023Quote: ... Two-hundred µL of 1x cell lysis buffer (Promega #E3971) was added and samples vortexed and incubated for 15 min ...
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bioRxiv - Plant Biology 2022Quote: ... Two micrograms of total RNA treated with DNAse (Promega, RQ1 #M6101) were used to synthesize cDNA (Promega ...
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bioRxiv - Cell Biology 2019Quote: ... Two days later the Dual-Glo Luciferase Assay System (E2940, Promega) was used to measure the luciferase activity of the cells on a bio-luminometer ...
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bioRxiv - Genetics 2022Quote: ... and two isoform1 mutant plasmids using FuGENE® HD (Promega, E2311) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... two reactions containing 40 μL TnT SP6 Quick Master Mix (Promega), 2 μL of 1 mM methionine and 1 μg of pCMV-Sport were incubated for 90 minutes at RT ...