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Citations for Promega :
451 - 500 of 1059 citations for Two Hybrid cDNA Library since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and cDNA was synthesized by GoScript reverse transcription system (Promega, Madison, WI, USA). Real-time quantitative RT-PCR was performed in duplicates on a CFX Automation System (Bio-rad) ...
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bioRxiv - Immunology 2021Quote: cDNA was prepared from 0.1 µg of total RNA using Random Primers (Promega) and M-MLV Reverse Transcriptase (Promega) ...
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bioRxiv - Cancer Biology 2022Quote: ... The extracted RNA was used for cDNA production using GoScript reverse transcriptase (Promega). Quantitative RT-PCR was performed using IQ SYBR green mix (Biorad ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was synthesized from normalized RNA input using m-MLV reverse transcriptase (Promega). qPCR was performed in duplicate with at least three biological replicates and normalized using the ΔΔCt method.
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bioRxiv - Molecular Biology 2023Quote: ... pCI Luc contains firefly luciferase cDNA cloned MluI / Xba I in pCI (Promega). pNLENG1-ES-IRES (a gift of D.N ...
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bioRxiv - Synthetic Biology 2023Quote: ... Purified RNA was reverse-transcribed into cDNAs with GoScript Reverse Transcription System (Promega) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2023Quote: ... and cDNA synthesis was performed using GoScript™ Reverse Transcriptase Kit (Promega #A5001). qRT-PCR reactions were performed using SensiFast SYBR no-rox kit (BIO-98020).
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bioRxiv - Cell Biology 2023Quote: ... and cDNA synthesis using the GoScript Reverse Transcription Mix Random Primers (A2800, Promega) were done according to the instructions of the manufacturer ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA synthesis was performed using ImProm-II™ Reverse Transcriptional System (A3800 - Promega) with 1 ug of RNA and RT-qPCR reactions were performed using GoTaq® qPCR Master Mix (A6002 - Promega) ...
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bioRxiv - Genomics 2022Quote: ... and cDNA synthesis using the GoScript RT Kit and oligo dT primer (Promega). To amplify the random 5’ and 3’ BC from the plasmid DNA or cDNA sample ...
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bioRxiv - Genomics 2023Quote: ... DNA and cDNA concentrations were determined using a Quantifluor® Assay (Promega®). Samples were diluted to 200 pg/μL or 100 pg/μL for Nextera XT (Illumina ...
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bioRxiv - Plant Biology 2022Quote: ... 10 μg total RNA was converted into cDNA using AMV Reverse Transcriptase (Promega) and oligo-dT primer ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-to-cDNA synthesis was performed using dNTPs and mmLVRT reverse transcriptase (Promega) according to manufacturer’s protocol and diluted to a standard concentration for mRNA expression analysis ...
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bioRxiv - Genomics 2023Quote: ... cDNA was generated via reverse transcription using the GoScript Reverse Transcription System (Promega) with 1 ul GoScript RT and 250 ng of RNA in a total volume of 20ul followed by incubation at 25 °C for 5min ...
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bioRxiv - Microbiology 2023Quote: ... the RNA was further reverse transcribed to cDNA using MMLV-RT (Promega, USA) and used for downstream mRNA qPCR assays ...
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bioRxiv - Immunology 2023Quote: ... cDNA was prepared by reverse transcription (GoTaq 2-Step RT-qPCR System, Promega) and amplified byPCR using SYBR® Green PCR Master Mix and ABI Prism7900HT sequence detection system (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2023Quote: ... and cDNA was prepared as per the GoScript™ Reverse Transcription System (Promega) protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... The full-length cDNA was ligated into the pGEM T-Easy vector (Promega) and sequenced using Sanger’s method to confirm the specificity of the product ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was synthesized from total RNA using M-MLV Reverse Transcriptase (Promega, M1701) using listed primers (Supplementary Table 1) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Samples were converted into cDNA by incubation with M-MLV Reverse Transcriptase (Promega) and dNTP Mix (Promega) ...
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bioRxiv - Immunology 2023Quote: ... and 1 mg of RNA was reversed into cDNA by reverse transcriptase ( Promega), which reverse transcribed the Moloney mouse leukemia virus ...
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bioRxiv - Biochemistry 2023Quote: ... Reverse transcription to cDNA was conducted using M-MLV Reverse Transcriptase (Promega, M1701) following the manufacturer’s instructions with subsequent confirmation of transcription using cyclophilin qPCR.
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bioRxiv - Molecular Biology 2023Quote: cDNA of extraction RNA was made using a commercial kit (Promega Company, USA), and RNA of samples was incubated with DNase I enzyme for two hours to remove any remains of DNA contamination ...
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bioRxiv - Genetics 2024Quote: ... cDNA was synthesized using the ImProm-II™ Reverse Transcription System (Promega, USA). The mRNA composition was established by PCR with plasmid-specific primers ...
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bioRxiv - Immunology 2023Quote: ... cDNA was prepared by reverse transcription (GoTaq 2-Step RT-qPCR System, Promega) and amplified by PCR using SYBR® Green PCR Master Mix and ABI Prism 7900HT sequence detection system (Applied Biosystems) ...
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bioRxiv - Cell Biology 2023Quote: ... Synthesis of cDNA was performed with M-MLV Reverse Transcriptase (Promega, cat.no. M1705) and random hexamer primers (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA conversion was performed using the GoScript™ Reverse Transcriptase kit (#A5001, Promega). Quantitative real-time PCR was conducted on a Bio-rad CFX96 Real-time system ...
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bioRxiv - Genomics 2023Quote: ... Long cDNA (> 2kb) fragments were selected with ProNex magnetic beads (Promega, WI, USA), and at least 80 ng were used to prepare the cDNA SMRTbell library ...
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bioRxiv - Microbiology 2020Quote: ... Clone libraries were generated with the pGEM®-T Easy Vector system (Promega) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... pH 7.5) and libraries were incubated with magnetic streptavidin bead-immobilised bromodomain (Promega) (200 nM ...
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bioRxiv - Bioengineering 2022Quote: ... Library sequencing preparation was performed using the GoTaq Master Mix from Promega (#M7433). D-(+)-Glucose was ordered from Sigma-Aldrich (#G8270) ...
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bioRxiv - Cancer Biology 2022Quote: Libraries were quantified by QuantiFluor assay run on a Glomax Explorer instrument (Promega) and sequenced on an Illumina NovaSeq 6000 platform to obtain 50 bp paired-end reads.
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized from 0.1 µg RNA using M-MLV Reverse Transcriptase (Promega, M3681) and oligo d(T)15 as primer following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was synthesized with the M-MLV Reverse Transcriptase (Promega, Madsion, WI, United States) and qPCR was done on the Rotor-Gene Q instrument (Qiagen ...
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bioRxiv - Genetics 2021Quote: Total RNA was reverse transcribed into cDNA using the GoScript Reverse Transcription System (Promega) and quantified by qPCR using SYBR GREEN mix (Vazyme Q111–02 ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was then reverse transcribed to cDNA using the AMV reverse transcriptase (Promega, M510F). qPCR experiments were performed to assess the levels of cDNA using primers targeting RPLP0 (FW ...
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bioRxiv - Immunology 2021Quote: ... PCR was performed with the generated cDNA and premixed 2× Taq polymerase solution (Promega) in an MJ Mini Thermal Cycler (BioRad) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... First strand cDNA was synthesized using 5μg pure RNA and M-MLV RT (Promega). qPCR was performed using pure cDNA and qPCR kit (Agilent technologies) ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 μg of RNA was reversed transcribed using cDNA synthesis reagents (Promega, Madison, WI). qPCR was performed using 6-carboxyfluorescein labeled TaqMan probes (Thermo ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The synthesized cDNA was inserted into a pGEM-T Easy vector (Promega, Madison, WI) and the full length was verified by sequencing.
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bioRxiv - Neuroscience 2020Quote: ... This was used for cDNA synthesis using the GO-Script Reverse Transcriptase kit (Promega) using 1 μl of random hexamer primers and 3.75 mM MgCl2 per 20-μl reaction ...
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bioRxiv - Neuroscience 2020Quote: ... RNA was reverse transcribed into cDNA using a ImProm-II Reverse Transcription System (Promega). cDNA was mixed with Luna Universal qPCR Master Mix (NEB) ...
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bioRxiv - Plant Biology 2021Quote: ... The cDNA template was used for PCR reaction with GoTaq Green Master Mix (Promega). The primers for RT-PCR are listed in Supplemental Table S1.
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bioRxiv - Plant Biology 2021Quote: ... cDNAs were prepared by GoScript™ Reverse Transcription System following manufactuere’s protocol (Promega, USA). All qRT-PCRs were performed in ABI 7300 real-time PCR machine (Applied Biosystem ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA synthesis was performed using M-MLV Reverse Transcriptase and RNasin Ribonuclease Inhibitor (Promega), and quantitative PCR reactions were done using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... cDNA synthesis was performed using random hexamers and ImProm-II™ Reverse Transcriptase (Promega). qPCR was performed with Power SYBR® Green (Thermo Fisher ...
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bioRxiv - Plant Biology 2020Quote: ... First-strand cDNAs were synthesised using Moloney Murine Leukemia Virus Reverse Transcriptase (Promega, www.promega.com). The quantification of gene expression was made by qRT-PCR ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized from 0.5 ug RNA using M-MLV Reverse Transcriptase (Promega, M3681) and oligo d(T)15 following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... and complementary DNA (cDNA) synthesis was performed using the M-MLV reverse transcriptase (Promega), deoxynucleotide triphosphates ...
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bioRxiv - Microbiology 2021Quote: ... cDNAs were synthesized from 1 μg of total RNA using AMV reverse transcriptase (Promega) and used directly for quantitative PCR (qPCR ...