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Citations for Promega :
351 - 400 of 1059 citations for Two Hybrid cDNA Library since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... were generated using PCR amplification of two DNA fragments and subsequent assembly PCR (using Pfu DNA polymerase from Promega, Mannheim, Germany) with the artificially inserted exon 9 sequence as region for annealing ...
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bioRxiv - Cell Biology 2023Quote: ... a 1839 bp region between two KpnI sites was amplified by PCR and subcloned into pGEM-T Easy (Promega, Madison, WI). This subclone was subjected to site-directed mutagenesis using the Q5 site-directed mutagenesis kit (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated at 37 °C for two days and the nLuc activity from the virus replication was measured by using Nano-Glo® Luciferase Assay System (Promega) following the manufacturer’s protocol using a plate reader (HT4 Biotek) ...
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bioRxiv - Microbiology 2023Quote: Insertion sites in individual clones were mapped using a two-step nested arbitrary PCR approach using 2X GoTaq master mix (Promega M7122). The first reaction (1X GoTaq master mix ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were digested at 47 °C for two hours with 600 ng of mass spectrometry grade Trypsin/LysC mix (Promega, V5113). The digested peptides were then eluted with two 35 µl increments of 0.2% formic acid in water and two more 40uL increments of 80% acetonitrile with 0.2% formic acid in water ...
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bioRxiv - Immunology 2021Quote: ... cDNA were synthesized from RNA (4 μg) using AMV Reverse Transcriptase (Promega). ...
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bioRxiv - Developmental Biology 2021Quote: ... The cDNA was reverse transcribed using M-MLV Reverse Transcriptase (Promega, M1701). The detailed primers used for qPCR are listed in Supplementary file 1A.
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bioRxiv - Immunology 2021Quote: ... cDNA was generated from 500ng of RNA using random hexamers (Promega, C1181) and the SuperScript III First-Strand kit (Themro Fisher ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was synthesized using M-MLV reverse transcriptase (M3682, Promega, Wisconsin, USA). Real-Time reactions were performed in triplicates using KAPA SYBR FAST qPCR Kit (2X ...
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Extensive introgression among North American wild grapes (Vitis) fuels biotic and abiotic adaptationbioRxiv - Evolutionary Biology 2021Quote: ... The obtained cDNAs were then purified with ProNex magnetic beads (Promega, WI) following the instructions in the Iso-Seq Express Template Preparation for Sequel and Sequel II Systems protocol (Pacific Biosciences ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA fragments were amplified using KOD Fx Neo (TOYOBO) or GoTaq (Promega) for 35 cycles under the following conditions ...
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bioRxiv - Cell Biology 2020Quote: New ORFs were generated from cDNA and first cloned into pGemTeasy (Promega). Primers were designed using primer346 to the boundary between the ORF and the UTR ...
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bioRxiv - Molecular Biology 2019Quote: ... and cDNA was generated using the Moloney Leukemia Virus Reverse Transcriptase (Promega), both according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2019Quote: ... Chicken α4 and β2 subunit cDNAs subcloned into pCI (Promega, Madison, WI) were kindly provided by Dr ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The isolated RNA was reverse transcribed into cDNA with MLV RT (Promega), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... and cDNA was synthesized with the GoScript™Reverse Transcription System (Promega). Quantitative PCR was performed using LightCycler® 480 SYBR® Green I Master (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... One microgram of total RNA was reverse transcribed into cDNA (A3500, Promega). Real-time quantitative PCR was performed using Fast SYBR Green master mix (Applied Biosystems ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was prepared from RNA (400 ng) by using random primers (Promega), GoScript buffer (Promega) ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA was synthesized by using AMV reverse transcriptase with random primers (Promega). TaqMan gene expression assays (Applied Biosystems ...
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bioRxiv - Developmental Biology 2021Quote: ... Synthesis of cDNA was performed using the GoScript Reverse Transcriptase kit (Promega) with random hexamer primers ...
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bioRxiv - Microbiology 2021Quote: ... We synthesized cDNA from 7 μl of RNA using m-MLV (Promega) with random hexamers for PCRs using Culex primers and ...
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bioRxiv - Plant Biology 2019Quote: ... followed by first-strand cDNA synthesis using M-MLV reverse transcriptase (Promega) and random hexamers (Roche ...
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bioRxiv - Physiology 2021Quote: ... cDNA was generated using M-MLV reverse transcriptase (Promega Corp., WI. USA) and random primers ...
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bioRxiv - Microbiology 2020Quote: ... Complementary DNA (cDNA) was prepared using Reverse Transcriptase Core kit (Promega Corporation) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... cDNA transfections were performed using JetPEI (Polyplus Transfection) or Fugene 6 (Promega) transfection reagents according to manufacturers’ instructions for 24h ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... we synthesized cDNA using the The GoScript™ Reverse Transcription System (Promega) and amplified a central region of the long-wavelength opsin using degenerated oligonucleotide primers 5’- TTGAAGCTTCARTTYCCNCCNATGAAYCC-3’ (forward ...
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bioRxiv - Microbiology 2020Quote: ... The cDNA was amplified by PCR using GoTaq G2 DNA Polymerase (Promega) with the following parameters ...
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bioRxiv - Immunology 2020Quote: ... cDNA synthesis was performed using GoScript™ Reverse Transcription System (Promega, USA). S-gene (Surface glycoprotein ...
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bioRxiv - Cell Biology 2022Quote: ... and subjected to cDNA synthesis with oligo(dT) reverse transcriptase (Promega; A2791) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... the slow-1 cDNA was cloned into pGEM-T Easy (Promega, #A1360) backbone ...
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bioRxiv - Genomics 2022Quote: ... The cDNAs were subsequently purified with ProNex magnetic beads (Promega, WI, USA) following the instructions in the Iso-Seq Express Template Preparation for Sequel and Sequel II Systems protocol (Pacific Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA synthesis was performed using the ImProm-II Reverse Transcription System (Promega). qRT-PCR was performed in triplicate with oligos targeting Scn1a and 18s mRNA using Titan HotTaq EvaGreen qPCR Mix 5x (BIOATLAS) ...
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bioRxiv - Neuroscience 2023Quote: ... and reverse transcribed into cDNA using the Promega RT Kit (Promega, A3500). RT-qPCR was performed using specific primer sequences for FOXG1 (F-CGTTCAGCTACAACGCGCTCA ...
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bioRxiv - Genetics 2023Quote: ... First-strand cDNA was then synthesized using M-MLV reverse transcriptase (Promega). The Open read frames (ORFs ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μg of RNA was reversed transcribed using cDNA synthesis reagents (Promega). qPCR was performed using the following 6-carboxyfluorescein-labeled TaqMan probes (Thermo) ...
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bioRxiv - Genomics 2023Quote: ... Reverse transcription was done using cDNA ImProm-II reverse transcription kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... mRNA was reverse-transcribed to cDNA by using oligo-dT primer (Promega) and M-MLV reverse transcriptase (Promega) ...
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bioRxiv - Immunology 2022Quote: ... Complementary DNA (cDNA) was synthesized using M-MLV retrotranscriptase kit (M1701, Promega) and random primers (B070-40 ...
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Analysis of spike protein variants evolved in a novel mouse model of persistent SARS-CoV-2 infectionbioRxiv - Microbiology 2023Quote: ... cDNA (50 μL) was synthesized using a Reverse Transcription System Kit (Promega). Expression of the RNA-dependent RNA polymerase (RdRP ...
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bioRxiv - Immunology 2023Quote: ... followed by cDNA synthesis using GoScript™ Reverse Transcription System (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... then it was reverse transcribed into cDNA using AMV reverse transcriptase (Promega). Finally ...
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bioRxiv - Developmental Biology 2023Quote: ... Catshark cDNA fragments were cloned into the pGEM-T Easy vector (Promega).
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bioRxiv - Immunology 2023Quote: ... and cDNA was synthesized using M-MLV reverse transcriptase (Promega, Madison, WI). Real time quantitative PCR analysis was performed using a QuantStudio 3 real time PCR system (Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... cDNA was then generated using a MMLV reverse transcriptase (Promega, Madison, WI). qPCR analysis was performed using a QuantStudio 3 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... then cDNA generated using M-MLV reverse transcriptase and random primers (Promega). Taqman (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... The first-strand cDNA was synthetized using M-MLV reverse transcriptase (Promega). The qRT-PCR was performed in a Quantstudio 3 thermocycler (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: cDNA was generated from 400ng of total RNA using Random Hexamers (Promega) and Ultrascript Reverse Transcriptase (PCR Bio ...
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bioRxiv - Genomics 2024Quote: ... Standard PCR was performed on cDNAs using the GoTaq DNA polymerase (Promega). All primer sequences can be found in Table S23.
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bioRxiv - Neuroscience 2024Quote: ... After cDNA conversion with a GoScript™ Reverse Transcriptase kit (#A5001, Promega), quantitative real-time PCR was performed on a Bio-rad CFX96 Real-time system ...
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bioRxiv - Synthetic Biology 2024Quote: ... and cDNA was synthesized using the GoScript reverse transcription kit A5001 (Promega), according to the manufactures’ instructions ...