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Citations for Promega :
9701 - 9750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... and 20 µL of the supernatant was used for the dual-luciferases assay measured in GloMax Navigator Plate Reader (Promega, GM2010) as specified by the manufacturer ...
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bioRxiv - Genomics 2023Quote: ... The lysates were generated by adding 100 µL 1X Passive Lysis Buffer included in the Dual-luciferases assay kit (Promega E1960) to the protoplasts and vigorously shaking at room temperature for 15 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Luciferase expression was assessed using a beetle luciferin-based assay system (Promega, Cat. No. PR-E1500), and luciferase activity was quantified with an LMax Luminometer (Molecular Devices) ...
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bioRxiv - Neuroscience 2023Quote: RNA from lyophilized cerebrum and brainstem samples was extracted using the Maxwell® RSC simplyRNA Tissue Kit/Instrument (Promega) with initial homogenization on a TissueLyser LT (Qiagen) ...
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bioRxiv - Neuroscience 2023Quote: ... alkylated in the dark with iodoacetamide and applied to S-Traps (mini; Protifi) for tryptic digestion (sequencing grade; Promega) in 50 mM TEAB ...
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bioRxiv - Neuroscience 2023Quote: ... Urea concentration was lowered to ¡2 M by addition of 100 mM Ammonium Bicarbonate and sequencing-grade modified trypsin 1.5 µg (Promega) was added to each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell number was measured at the indicated time intervals with CellTiter-Glo® Luminescent Cell Viability Assay (Promega #G7571) following manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... LgBiT and lytic substrate (Promega) were added 1:100 and 1:50 ...
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bioRxiv - Neuroscience 2023Quote: ... The Cell Titer Glo 2.0 (Promega) viability assay was performed after 24 h of treatment ...
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bioRxiv - Neuroscience 2023Quote: ... Blots were then incubated with blotting buffer containing 1:200 LgBiT for 1-2 h with rocking at RT (N2410, Promega). Next ...
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bioRxiv - Neuroscience 2023Quote: ... Fifty microliters of stopping solution was added to each well and the absorbance at 490 nm was read on a GloMax (Promega).
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bioRxiv - Neuroscience 2023Quote: Plasmid constructs were acutely transfected into HEK293 cells using Viafect reagent (E4981; Promega, Madison, WI), following the manufacture’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... and luciferase reported activity was measured with an Infinite M200 Pro Microplate Reader luminometer using the Dual-Luciferase assay system (Promega). Background luminosity was subtracted from un-transfected cells and luciferase readings were normalized by dividing the reported gene firefly luciferase by the reference Renilla luciferase activity ...
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bioRxiv - Molecular Biology 2023Quote: ... viability was measured using CellTiter-Blue assay (G8081/2, Promega) following the protocol of the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: HeLa or HEK cells were cultured for at least one day and transfected using FUGENE HD reagent (Promega) according to manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... using the CellTiter-Blue assay (G8081/2, Promega) following the protocol of the manufacturer using an EnVision multimode plate reader (PerkinElmer) ...
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bioRxiv - Molecular Biology 2023Quote: GloSensor cAMP assay was used to detecte the downstream Gαi signal of MCHR1 using GloSensor cAMP Reagent (Promega). HEK293 cells were cultured in DMEM/ high Glucose supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... HaloPROTAC3 (Promega, GA3110), HaloPROTAC-E (MedChem Express ...
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bioRxiv - Molecular Biology 2023Quote: The pGEM®-T Easy Vector System (Promega) was used according to the manufacturer protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... beads were resuspended in 100 μL of 25 mM NH4HCO3 and digested by adding 0.2 μg of trypsine/LysC (Promega) for 1 h at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... 150 μg/well of either pGL3-B.HTSS2KB or pGL3-Intron3-DSCR1 reporter construct was transfected with 150 μg/well of pCDNA3-ΔCnA,and 300 μg/well pEF-BOS-hNFATp and/or 150 until 600 μg/well of pEGFP-VIVIT.7,17 pRL-TK (Promega; xx μg/well), containing the thymidine kinase promoter driving Renilla luciferase ...
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bioRxiv - Molecular Biology 2023Quote: Reverse transcription of the total RNA was performed using M-MLV and RNAsin (Promega) following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: A construct bearing 2 kb of the murine Bigheart promoter (pGL3-B.HTSS2KB) was subcloned as a HindIII and SacI fragment into the pGL3-Basic vector (Promega). pGL3-Intron3-DSCR1 harbors the complete (875 bp ...
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bioRxiv - Molecular Biology 2023Quote: ... Extracted RNA was purified using RQ1 RNase-free DNase (Promega) then cDNA generated using M-MLV reverse transcriptase and random primers (Promega) ...
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bioRxiv - Molecular Biology 2023Quote: ... then cDNA generated using M-MLV reverse transcriptase and random primers (Promega). Taqman (Thermo Fisher Scientific ...
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Modifications in the T arm of tRNA globally determine tRNA maturation, function and cellular fitnessbioRxiv - Molecular Biology 2023Quote: ... and firefly and Renilla luciferase were measured using the Dual-Luciferase® Reporter Assay System (Promega) as per the manufacturer’s instructions with a Spectramax i3 plate reader (Molecular Devices) ...
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bioRxiv - Molecular Biology 2023Quote: Luciferase assays were performed using the Dual-Luciferase Reporter Assay System (Promega). Frozen pellets were resuspended in 300 μL of 1X Passive Lysis Buffer (PLB ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA integrity was assessed by electrophoresis and genomic DNA was eliminated with RQ1 DNAse (Promega, Madison, WI, USA) and subsequent chloroform extraction and ethanol precipitation ...
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bioRxiv - Molecular Biology 2023Quote: ... a pGEM-T Easy vector (Promega, Madison, WI, USA) with the blasticidin cassette flanked by a fragment of the 5’ UTR and 3’ UTR of the SUMO gene ...
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bioRxiv - Molecular Biology 2023Quote: ... 4μg of total RNA spiked with 1ng/μL of Luciferase Control RNA (Promega #L4561) as a negative uncapped control ...
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bioRxiv - Molecular Biology 2023Quote: ... and lysates were assayed for luciferase activity using Bright-Glo™ (Promega) on a 96-well using The VICTOR3™ Multilabel Plate Reader (PerkinElmer LAS Germany GmbH).
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bioRxiv - Microbiology 2023Quote: ... the supernatants were removed and 1X 50 μl of Glo Lysis Buffer (Promega, USA) were added to each well and incubated for at least 10 min ...
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bioRxiv - Microbiology 2023Quote: ... NanoGlo luciferase substrate (Promega, USA) was diluted 1:50 in NanoGlo Buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by DNase digestion using RQ1 RNase free DNase (PROMEGA, USA) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Clean T7 templates were used to generate dsRNA with the T7 RiboMAX Express RNAi System (#P1700, Promega). Resulting dsRNA was resuspended in nuclease-free water and quantified on a Denovix DS-11.
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Broad variation in response of individual introns to splicing inhibitors in a humanized yeast strainbioRxiv - Molecular Biology 2023Quote: ... 0.5 μL 40 U/μL RNAse inhibitor (RNAsIN, Promega), and 0.5 μL 200 U/μL SuperScript III RTase (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... Firefly and Renilla luciferase activities were measured in duplicates at 48 hpt using the Dual-Glo Luciferase Assay system (Promega) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... following the manufacturer’s instructions (DeadEnd™ Fluorometric TUNEL System – Promega).
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Nucleolar Pol II interactome reveals TBPL1, PAF1, and Pol I at intergenic rDNA drive rRNA biogenesisbioRxiv - Molecular Biology 2023Quote: ... Tryptic digestion was performed by incubating beads with 1 μg MS-grade TPCK trypsin (Promega, Madison, WI) dissolved in 200 μl of 50 mM ammbic (pH 8.3 ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA (4 μg) was reverse-transcribed using oligo-dT and the Reverse Transcription System (Promega). cDNA samples were diluted 1:10 and 5 μl of each sample were used for PCR reactions (final volume of 20 μl ...
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bioRxiv - Molecular Biology 2023Quote: ... mouse anti-HaloTag (Promega, G921A), rabbit anti-DDX6 (Bethyl ...
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bioRxiv - Molecular Biology 2023Quote: HEK293-µOR cells were transfected with a plasmid encoding pGloSensor-20F cAMP reporter (Promega). Cells were harvested 24 h post transfection and resuspended at a 1.5x10^6 live cells/ml in assay media (DMEM without phenol red or FluoroBrite DMEM ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 ng pCas9 mCherry Frame +1 and 100ng pCRISPaint HaloTag-Puro donor plasmids were transfected using ViaFect (Promega E498A). Three days post-transfection cells were sub-cultured into media supplemented with puromycin (0.8 µg/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... and the HBB 5′ UTR (ENST00000335295.4) commercially generated (IDT) sequences were each inserted into the pNL1.1 NanoLuc luciferase reporter plasmid (Promega, GenBank Accession Number JQ437370) downstream of a T7 promoter using overlap-extension PCR with Q5 High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... The original minimal promoter from the pGL4.23 construct (Promega) was first removed via digestion with BglII and NcoI and the plasmid backbone was purified ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were in-gel digested using 1µg trypsin (Trypsin Gold, Promega)(37) ...
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bioRxiv - Molecular Biology 2023Quote: ... Luminescence in the wells was counted using the Renilla-Glo® luciferase assay system (Promega, Madison, Wisconsin) according to the manufacturer’s specifications ...
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bioRxiv - Microbiology 2023Quote: ... was amplified from complementary DNA synthesized by reverse transcriptase starting from RNA of HAP1 WT cells (GoScriptTM Reverse Transcriptase, Promega, Madison WI, USA). HMBS_232 was cloned into pCMV-puroR lentiviral plasmid using the primers listed in Table S6 and NEBuilder HiFi DNA Assembly Master Mix (New England BioLabs) ...
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bioRxiv - Microbiology 2023Quote: LEC viability was determined using the CellTiter-Glo Luminescent Cell Viability Assay (Promega). Uninfected and KSHV-infected LECs were seeded at a density of 7,500-10,000 cells per well of a white 96-well plate ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were digested overnight at room temperature with 0.4 µg of sequencing grade modified trypsin (Promega, #V5111) in 40 µl Hepes/NaOH ...