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Citations for Promega :
9651 - 9700 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... In-gel digestion was performed using Trypsin Gold (Promega) and ProteaseMAXTM surfactant (Promega ...
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bioRxiv - Microbiology 2023Quote: ... according to the manufacturers’ instructions and linearized by restriction enzyme BamHI (Promega) before purification with the QIAquick PCR purification kit (Qiagen) ...
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bioRxiv - Microbiology 2023Quote: ... As a qPCR standard the recombinant plasmid pBELX-1 (Bellanger et al., 2014) was extracted using the Wizard plus SV Miniprep DNA Purification System (Promega, Madison, WI, USA) according to the manufacturers’ instructions and linearized by restriction enzyme BamHI (Promega ...
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bioRxiv - Microbiology 2023Quote: ... Cells were infected the next day with culture supernatants and lysed 48 hpi followed by measuring luminescence using the Steady-Glo luciferase assay system (Promega) per the manufacturer’s recommendation and a Biostack4 (BioTek ...
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bioRxiv - Molecular Biology 2023Quote: ... 15 mg of His-Tev-HaloTag-(3C)-human γ-TuNA was coupled to Halo Magne beads (Promega). Xenopus laevis egg extract was prepared by standard methods28 ...
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bioRxiv - Molecular Biology 2023Quote: ... Parental plasmids were selectively linearised from the PCR product via digestion with Dpnl (Promega) in lx MULTI-CORE buffer for 1hr at 37°C and the digestion product was immediately transformed into Stbl3 competent bacteria.
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bioRxiv - Microbiology 2023Quote: ... 2 μg of env clone was co-transfected with 4 μg of the pNL4.3-ΔEnv-vpr+-luc+ into 293T cells in a T25 flask using the FuGENE6 transfection reagent (Promega). The cells were cultured at 37°C for 6 hours before the medium was completely replaced with fresh medium ...
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bioRxiv - Microbiology 2023Quote: ... or 5 µL qPCR plasmid pJEB029 (diluted serially in lambda DNA (Promega)) were added ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA was purified using a Wizard Genomic DNA Purification Kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... using the TnT Coupled Reticulocyte Lysate System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... using the TnT Coupled Reticulocyte Lysate System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Halo-Magne beads (Promega: G7287) were coated in purified HaloTag-PreScission-humanγTuNA ...
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bioRxiv - Molecular Biology 2023Quote: ... The gel slices were then dried again and treated with an ice-cold solution of 12.5 ng/μL trypsin (Promega, Madison, WI) in 100 mM ammonium bicarbonate ...
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bioRxiv - Molecular Biology 2023Quote: ... One tube was further digested with thermolysin (Promega, Madison, WI) by resuspending the tryptically digested peptides with a solution containing 50 mM Tris-HCl pH 8 and 0.5 mM calcium chloride and adding 1 μg of thermolysin ...
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bioRxiv - Molecular Biology 2023Quote: ... and treated with 2 μg trypsin (Promega, v511c), shaking overnight at 700 rpm ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were cloned into the plasmid pGEMT easy (Promega). For each gene ...
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bioRxiv - Microbiology 2023Quote: HFF or HEK293 cells (3 × 104 cells per well of a 24-well plate or 1 × 105 cells per well of a 6-well plate) were reverse transfected with 0.5 μg of pCW57-CMV-KDEL-mCherry-GF using Fugene HD (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... in TBS-T followed by a washing step for 30 min with TBS-T and a final 60 min incubation with with 1:10.000 horseradish peroxide-conjugated anti-rabbit IgG (Promega) in TBS-T ...
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bioRxiv - Microbiology 2023Quote: pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega) was linearized by PCR with primers that insert two fully complementary binding sites (“perfect target sites” ...
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bioRxiv - Microbiology 2023Quote: ... and plasmids (pX-FLAG-hAGO2 and pX-FLAG-HrAGO1) were purified using PureYield Plasmid Miniprep System (Promega).
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bioRxiv - Microbiology 2023Quote: ... 0.25 µl 1 M MgCl2 and 0.3 µl Rnasin ribonuclease inhibitor (Promega, 0.4 u/µl) in a final volume of 30 µl at 25°C overnight ...
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bioRxiv - Microbiology 2023Quote: Host cell toxicity was measured using CellTiter AQueous assay (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... cDNA was then generated using a MMLV reverse transcriptase (Promega, Madison, WI). qPCR analysis was performed using a QuantStudio 3 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... 2 μg RNA was used to synthesize first-strand cDNA using Moloney mouse leukemia virus (M-MLV) (Promega). The amplification of specific PCR products was detected using SYBR Premix Ex TaqTM II (Vazyme) ...
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bioRxiv - Genomics 2023Quote: ... we followed a CRISPR knock-in protocol to endogenously insert a HiBiT tag (Promega 88) at the 3’ terminus of the IFNAR2-S mRNA (Fig ...
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bioRxiv - Genomics 2023Quote: ... We used the HaloTag Mammalian Pull-Down and Labeling Systems kit (Promega; #G6500) to identify protein interacting with IFNAR2 in stimulated and unstimulated conditions following manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... We added Nano-Glo Luciferase Assay Substrate and LgBiT protein (Promega) and imaged using the ImageQuant LAS4000 Imager.
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bioRxiv - Genomics 2023Quote: ... and then cloned into the pHTC HaloTag CMV-neo Vector (Promega #G7711) using In-Fusion cloning (Takara Bio #638947) ...
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bioRxiv - Genomics 2023Quote: ... and heterozygous and homozygous clones were identified and screened using the Nano-Glo HiBiT Lytic Detection System (Promega #N3030). We confirmed in-frame insertions by PCR ...
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bioRxiv - Genomics 2023Quote: ... we used the ProTEV Plus enzyme (Promega; #V6101) to cleave the HaloTag from the bait protein following the protocol provided with the HaloTag Mammalian Pull-Down and Labeling Systems ...
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bioRxiv - Immunology 2023Quote: ... The luciferase activity in cell lysates was measured using the Nano-Glo Luciferase Assay System (Promega) and GloMax system (Turner BioSystems) ...
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bioRxiv - Genomics 2023Quote: ... Transfection of plasmid DNA (2.5μg vector + 1μg of transposase plasmid) was performed using the FuGENE transfection reagent (Promega #E5911) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... the HaloTag coding sequence was amplified from the pHTC HaloTag CMV-neo Vector (Promega #G7711). Cloning reactions were transfected into NEB stable competent E ...
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bioRxiv - Genomics 2023Quote: ... and stained for 1hr at room temperature with the permeable Janelia Fluor HaloTag Ligand (JFX549; Promega #GA1110).
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bioRxiv - Genomics 2023Quote: ... We also assessed cell viability of each sample in triplicates through a luminescence assay of ATP (CellTiter-Glo 2.0 Assay, Promega #G9242) following manufacturer’s instructions.
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bioRxiv - Genomics 2023Quote: Promoters were amplified from mouse genomic DNA using primers listed in table S1 and cloned into pGL3-Basic (E1751, Promega) using SacI and BglII.
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bioRxiv - Genomics 2023Quote: ... the automated Maxwell Blood purification kit (Promega), or the QIAamp Blood Midi kit protocol (Qiagen) ...
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bioRxiv - Immunology 2023Quote: ... and 1 mg of RNA was reversed into cDNA by reverse transcriptase ( Promega), which reverse transcribed the Moloney mouse leukemia virus ...
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bioRxiv - Immunology 2023Quote: ... Cell samples were collected at the indicated times and lysed or assayed using the Dual-Luciferase® Reporter Gene (DLR™) Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and an apoptosis assay using Caspase-Glo® 3/7 reagent (G8091, Promega, Canada), as per manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... and O1v1 were amplified from purified NTUH gDNA (Wizard Genomic DNA Purification Kit, Promega) using primers O2a_cluster_1F/O2a_cluster_1R ...
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bioRxiv - Immunology 2023Quote: ... cDNA was synthesized by the ImProm-II™ Reverse Transcriptase system (Promega, Cat# A3801) using 100 ng RNA per reaction ...
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bioRxiv - Genomics 2023Quote: ... we used the Nano-Glo HiBiT Blotting System (Promega) to detect protein following SDS-PAGE and transfer ...
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bioRxiv - Genomics 2023Quote: ... STR analysis was performed with PowerPlex 21 System (Promega DC8902) following the manufactory’s protocol ...
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bioRxiv - Genomics 2023Quote: ... cells were washed once with cold 1× PBS and the luciferase activities were measured with Perkin Elmer Envision using Promega Dual Luciferase Assay kit (Promega, E1960). The firefly luciferase activity was normalized to renilla luciferase activity for each well ...
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bioRxiv - Immunology 2023Quote: DNA in the HHMX-stimulated supernatants of THP1 dual reporter cells was quantified using the QuantiFluor dsDNA Detection Kit from Promega (catalog # E2670) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... and DNA was isolated and purified using the Wizard SV Genomic DNA Purification System (Promega, WI, USA, catalog # A2920) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... 5% CO2 for 24 hrs, after which luciferase activity (Relative Light Units, RLU) was measured in cell lysates using the Bright-Glo system (Promega, Madison, WI) with a Bio-Tek II plate reader ...
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bioRxiv - Immunology 2023Quote: RNA isolation from the cultured THP1 dual reporter cells was performed by using ReliaPrep RNA Cell Miniprep Kit (Promega, USA, catalog # Z6011) according to the instructions of the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... according to the instructions of Non-Radioactive Cytotoxicity Assay Kit (Promega, catalog # G1780). The positive control (100% cytotoxicity ...