Labshake search
Citations for Promega :
7401 - 7450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... siRNA transfections were carried out using FuGene reagent (Promega) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... Renilla luciferase activity was determined using the Luciferase Assay Systems (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... the pellet of total RNA was dissolved in RNase-free water (Promega, Madison, WI, USA) and stored at −80 °C.
-
bioRxiv - Molecular Biology 2023Quote: ... SYBR Green-based RT-qPCR reactions were set up using GoTaq qPCR Master Mix (Promega) and 20 ng of input cDNA per each 10-μL reaction ...
-
bioRxiv - Biophysics 2023Quote: ... The gel was then dried and rehydrated with trypsin (Promega) at an estimated 1:20 w/w ratio in 50mM ammonium bicarbonate ...
-
bioRxiv - Bioengineering 2023Quote: ... Samples were incubated with CellTiter-Blue (Promega, Madison, WI, USA) at 20% sample volume according to manufacturer’s instructions for 4 hours ...
-
bioRxiv - Bioengineering 2023Quote: Caspase 3/7 activity was determined using the Caspase-Glo 3/7 Assay Kit (Catalog No. G8091, Promega) for each sample according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2023Quote: ... The total ATP contents in static and dynamic cultured Hep3B cell spheroids were measured with the CellTiter-Glo® assay kit (Promega, G9681). Briefly ...
-
bioRxiv - Bioengineering 2023Quote: ... tumor fragments (200-300 mg) were lysed for 1 h on ice with agitation in 1X Reporter Cell Lysis Buffer (Promega) and then centrifuged at 14,000 x g for 15 min at 4°C ...
-
bioRxiv - Bioengineering 2023Quote: ... Lysates (20 mg per well) were assessed in 96-well plates using 90 μl reconstituted Luciferase Assay Substrate (Promega) according to the manufacturer’s directions ...
-
bioRxiv - Bioengineering 2023Quote: ... luciferase activity was measured using a Bight-Glo™ Luciferase Assay System (Promega, Madison, WI) as per the manufacturer’s protocol.
-
bioRxiv - Bioengineering 2023Quote: ... Firefly and Renilla Luciferase activities in the lysate were then determined with a Dual-Luciferase Reporter Assay System (Promega) and a GloMax explorer plate reader (Promega ...
-
bioRxiv - Bioengineering 2023Quote: ... and a GloMax explorer plate reader (Promega) following the manufacturer instructions ...
-
bioRxiv - Immunology 2023Quote: ... The reaction was stopped by KPL TBM Stop Solution (SeraCare, #5150-0021) and plates were read at 450nm on GloMax Explorer Multimode Microplate Reader (Promega).
-
bioRxiv - Immunology 2023Quote: ... cells were washed 3X with prewarmed HBSS and fluorescence was read on GloMax Explorer Multimode Microplate Reader (Promega).
-
bioRxiv - Cancer Biology 2023Quote: ... using Cell Titre Glo 2.0 Cell Viability Assay (Promega) per manufacturer’s instructions.
-
bioRxiv - Bioengineering 2023Quote: ... were: β-tubulin (1:1500, TUJ1, Promega, G7121), Sox10 (1:300 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Assessment of DNA fragment length was performed by electrophoretic separation in a 0.4% agarose gel with DNA ladder (Promega, WI, USA).
-
bioRxiv - Cancer Biology 2023Quote: ... 20µL of the lysate was revealed using the Dual-Glo luciferase assay system following the manufacturer’s protocol (Promega, E1910). Luminescence was measured using a FLUOstar Optima plate reader (BMG).
-
bioRxiv - Cancer Biology 2023Quote: ... 30μg of proteins were digested with 1µg Trypsin sequencing grade (Promega) for 14h at 37°C ...
-
bioRxiv - Developmental Biology 2023Quote: ... with 1 ug of RNA and RT-qPCR reactions were performed using GoTaq® qPCR Master Mix (A6002 - Promega). Analyses were carried out in the QuantStudio™ 5 Real-Time PCR System ...
-
bioRxiv - Developmental Biology 2023Quote: ... cDNA synthesis was performed using ImProm-II™ Reverse Transcriptional System (A3800 - Promega) with 1 ug of RNA and RT-qPCR reactions were performed using GoTaq® qPCR Master Mix (A6002 - Promega) ...
-
bioRxiv - Cell Biology 2023Quote: ... along with the NBT and BCIP (Promega) detection system.
-
bioRxiv - Cell Biology 2023Quote: ... cloned in pGEM-T vector (Promega) and sequenced (Macrogen) ...
-
bioRxiv - Cell Biology 2023Quote: ... RNA extraction was performed according to the manufacturer’s instructions and cDNA was obtained by means of MMLV reverse transcriptase (Promega) using oligo dT with the protocol provided with the enzyme ...
-
bioRxiv - Cell Biology 2023Quote: ... using HC T4 Ligase and rapid ligation buffer (Promega). Sequences of all recombinant plasmids were verified by direct sequencing ...
-
bioRxiv - Cell Biology 2023Quote: Heat shock-induced cytotoxicity was determined using the LDH-Glo cytotoxicity assay (Promega, catalog number: J2380) according to the manufacturer’s guidelines ...
-
bioRxiv - Microbiology 2023Quote: ... using the GoTaq® Probe 1-Step RT-qPCR System (Promega). Methods for the generation of the 18S and sgE RNA standards have been outlined previously.31 Both PCR products were serially diluted to produce standard curves in the range of 5 x 108 - 5 copies/reaction via a 10-fold serial dilution ...
-
bioRxiv - Microbiology 2023Quote: ... GoTaq® Probe 1-Step RT-qPCR System was purchased from Promega (US). SARS-CoV-2 (2019nCoV ...
-
bioRxiv - Microbiology 2023Quote: ... using the GoTaq® Probe 1-Step RT-qPCR System (Promega). Quantification of 18S RNA utilised previously described primers and probe sequences,30 and were used at 300 nM and 200 nM ...
-
bioRxiv - Microbiology 2023Quote: The viral RNA derived from the lung and nasal turbinate samples was quantified using a protocol for quantifying the SARS-CoV-2 sub-genomic E gene RNA (sgE)29 using the GoTaq® Probe 1-Step RT-qPCR System (Promega).
-
bioRxiv - Cancer Biology 2023Quote: ... Intracellular levels of NAD/NADH were measured using the NAD/NADH-GloTM quantitation kit ((Promega, G9071) according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2023Quote: ... live-cell renilla luciferase (Rluc) substrate (EnduRen, Promega) was added ...
-
bioRxiv - Biophysics 2023Quote: ... or purified LgBiT-substrate cocktail (Promega). After incubation with appropriate HRP-tagged secondary antibodies and chemiluminescent substrate (Thermo Fisher) ...
-
bioRxiv - Biophysics 2023Quote: ... equal volumes of HiBiT-N VLPs and hACE2-LgBiT EVs were mixed with nanoluc substrate (cat#N2420, Promega) and trypsin (Sigma ...
-
bioRxiv - Biophysics 2023Quote: ... The pCMV-LgBiT expression plasmid was purchased from Promega. pcDNA3.1-hACE2-C9 was obtained from Dr ...
-
bioRxiv - Developmental Biology 2023Quote: ... Concentration and fragment size of amplified cDNA was measured with QuantiFluor® dsDNA (Promega) and Fragment Analyzer (Advanced Analytical ...
-
bioRxiv - Cell Biology 2023Quote: ... 2 µL of 50% Cell-Titer Glo (Promega cat G7573) diluted in water was added to the cells and luminescence measured on an EnVision Plate Reader (PerkinElmer).
-
bioRxiv - Cell Biology 2023Quote: ... cytochrome P450 3A4 activity (CYP3A4) was measured using a luciferin-IPA kit (Promega cat V9001) following the lytic protocol with 3 μM IPA ...
-
bioRxiv - Animal Behavior and Cognition 2023Quote: ... followed by overnight modified trypsin digestion (Promega) at a ratio of 1/100 (enzyme/proteins ...
-
bioRxiv - Cell Biology 2023Quote: ... supplemented with or without 50 μM adenosine 5′-triphosphate (ATP) at 30 °C for 30 minutes followed by kinase assays using ADP-Glo Kinase Assay kit (Promega). Data were analyzed using GraphPad Prism 9 ...
-
bioRxiv - Cell Biology 2023Quote: ... were kindly provided by Promega. N-terminal NLuc orientations were used for CDKL5 ...
-
bioRxiv - Immunology 2023Quote: ... 30 μl of 0.02 μg/μl of trypsin (Promega V5111) solution was added to the gel pieces for 20 min followed by addition of 60 μl solution containing 40 mM NH4HCO3 / 10% ACN to completely cover the gel pieces and the samples were incubated at 37°C for 18 h ...
-
bioRxiv - Developmental Biology 2023Quote: ... Luminescence was measured in 96-well format using Nano-Glo Luciferase (Promega) and a Modulus luminescence plate reader.
-
bioRxiv - Developmental Biology 2023Quote: ... gBlocks were inserted using sequence-and ligase-independent cloning (58) upstream of the coding sequence for a secreted nano-luciferase following digestion of vector pNL2.3 (Promega) using EcoRV and HindIII ...
-
bioRxiv - Neuroscience 2023Quote: ... exposed to ProteasomeGlo® (Promega, G8660) and incubated for 30 minutes at RT ...
-
bioRxiv - Biophysics 2023Quote: ... and the suspension was applied on Flex-Column (420400-2510) connected to Vac-Man vacuum manifold (Promega).
-
bioRxiv - Bioengineering 2023Quote: ... cells were stained with JF549 conjugated Halotag ligands (Promega) at 200nM concentration in 2% FBS/PBS for 20 min at RT ...
-
bioRxiv - Bioengineering 2023Quote: ... Samples were then digested first with 6.6μg of LysC at room temperature overnight followed with 1.65ug sequencing grade trypsin (Promega) for 6 hours at 47°C ...
-
bioRxiv - Bioengineering 2023Quote: Cell viability was determined using the CellTiter-Glo Luminescent Cell Viability Assay Kit (Catalog No. G7570, Promega) for each sample according to the manufacturer’s instructions ...