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Citations for Promega :
701 - 750 of 1564 citations for 2x SYBR Green qPCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The amplification was performed in 50 µl mixture containing 20 µl GoTaq®Green Master Mix (Promega, Madison, WI, United States), 1 µl of each primer (10 µM) ...
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bioRxiv - Synthetic Biology 2022Quote: ... and the integration of antibiotic resistance cassettes or reporter genetic constructs in the genome was confirmed by PCR under standard conditions using the GoTaq Green Master Mix (Promega, USA). The strains used and generated herein are shown in Table S1 ...
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bioRxiv - Neuroscience 2024Quote: ... One µL of lysate was then added to make 20µL PCR reactions with GoTaq G2 Green Master Mix (Promega, Cat# M7823) and 1µM forward and reverse primers (Table 1) ...
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bioRxiv - Genetics 2024Quote: ... 500–800 nt amplicons were amplified from genomic DNA from individual insertion lines through single fly PCR using GoTaq green master mix (Promega M7122).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and a final extension at 72 °C for 10 min using GoTaq® G2 Hot Start Green Master Mix (Promega, USA). The PCR products of each gene were then separated on 2% agarose gels containing SYBR® Safe DNA Gel Stain (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... and the DNA fragments encoding the designs were amplified following a colonyPCR protocol using GoTaq® Green DNA polymerase master mix (#M7122; Promega) and T7 reverse and forward primers ...
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bioRxiv - Molecular Biology 2023Quote: ... The PEG10-5’AmC6-modified primers were used for the amplification of the donor template using GoTaq® Green Master Mix DNA polymerase (Promega). The SmUbi-EGFP-SmUbi construct with the 600 bp HA sequence (21 ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 µl of cDNA per sample were used and qRT-PCR was performed using the GoTaq® qPCR Master Mix (Promega, A6001) on a LightCycler 96 (Roche) ...
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bioRxiv - Molecular Biology 2020Quote: ... Real Time PCR was done with unique oligonucleotide primers targeting ABCB11 gene using GoTaq® qPCR Master Mix (Promega Cat. No. A6001) following ‘manufacturer’s instructions on a Veriti Thermo Cycler from Applied Biosystems Waltham ...
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bioRxiv - Genetics 2024Quote: ... The qRT-PCR reactions were conducted in a 10 μL volume using the GoTaq qPCR Master Mix chemistry (Promega, Madison, Wisconsin, USA) and a CFX384 Touch Real-Time PCR Detection System (BioRad ...
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bioRxiv - Immunology 2023Quote: ... 4 µl of cDNA per sample were used and qRT-PCR was performed using the GoTaq® qPCR Master Mix (Promega, #A6001) on a LightCycler 96 (Roche) ...
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bioRxiv - Molecular Biology 2020Quote: ... 50ng of each DNA sample were used per qPCR reaction (GoTaq qPCR Mix, Promega), and an untransfected control was used to discard plasmid contamination ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR was performed in GoTaq Master Mix (Promega) according to standard procedures ...
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bioRxiv - Synthetic Biology 2021Quote: ... using the OneTaq hot start master mix (Promega) and corresponding primers (Supplementary Table 1) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 3.5 µl of GoTaq Colorless Master Mix (Promega), 0.6µl of forward and reverse primers ...
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bioRxiv - Microbiology 2021Quote: ... 1 unit of GoTaqG2 master mix (Promega UK), 5 μL of DNA sample and milliQ water to reach a final reaction volume of 25 μL ...
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bioRxiv - Microbiology 2020Quote: ... PCR was performed with GoTaq Master Mix (Promega) with 1ul cDNA template at 1:20 dilution ...
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bioRxiv - Immunology 2020Quote: ... which was performed with GoTaq master mix (Promega).
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bioRxiv - Microbiology 2022Quote: ... or GoTaq Master Mix (Promega, Madison, WI, USA). Vectors for heterologous expression ...
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bioRxiv - Microbiology 2024Quote: PCR was performed using GoTaq Master Mix (Promega) using 5 ng of DNA extracted from RS collected after growth on MHA containing 0 or 32 μg/mL cefiderocol ...
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bioRxiv - Developmental Biology 2024Quote: ... and also with PCR Master Mix (M7502, Promega), Taq polymerase (EP0402 ...
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bioRxiv - Microbiology 2024Quote: ... and GoTaq Master Mix (Promega; Madison, WI, USA) for a total volume of 25 µl per sample ...
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bioRxiv - Plant Biology 2024Quote: ... using GoTaq® qPCR Master MixA6001 (Promega, Madison, WI, USA) with the following protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... Quantitative PCR was performed using SYBR-green mastermix (Promega) and run in a Prism 7900 HT instrument (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... template and Sybr green DNA dye (Promega, Madison, USA). Thermalcycler conditions were as follows ...
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bioRxiv - Developmental Biology 2023Quote: ... and SYBR Green fluorescent dye (Promega, Madison, WI, USA) were used ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunoprecipitated protein–DNA complexes and 5% input were analyzed by qRT-PCR using GO taq QPCR master mix (Promega, A6002, lot no. 0000385100) in triplicate using primers specific for PFKFB3 HREs ...
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bioRxiv - Cell Biology 2024Quote: ... RT-qPCR analysis was done on a QuantStudio™ 5 Real-Time PCR System in a final volume of 20 µl containing 10 µl of GoTaq qPCR Master Mix (Promega, Cat No. PRA6001), 2 µl of the forward plus reverse primers (final primer concentration ...
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bioRxiv - Cell Biology 2020Quote: ... according to manufacturer’s instructions and analyzed for the presence of the wildtype and knock-in Lmna allele by PCR using the GoTaq green master mix (Promega, Madison, WI) and the following primer pairs (see also ‘Primer and sgRNA sequences’):
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bioRxiv - Molecular Biology 2019Quote: ... PCR was performed using primers spanning 75-150 base pairs (Table S5) for each transcript using GoTaq® Green Master Mix (Promega, M7122) for 35 cycles.
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bioRxiv - Bioengineering 2021Quote: ... Presence of endogenous and/or exogenous Nix in the genome was assessed by PCR using GoTaq® Green Master Mix (M712, Promega, France) according to the manufacturer’s recommendations with the Nix-833 published primers 25 and primer EM1926-EM1927 (Supplementary Table 1) ...
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bioRxiv - Cancer Biology 2022Quote: Synthesized cDNA as described above was used to measure levels of unspliced and spliced XBP1mRNA were measured by regular PCR performed with GoTaq Green Master Mix (Promega cat#: M7123) on a T100 Thermo Cycler (Bio-Rad cat# ...
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bioRxiv - Physiology 2019Quote: An aliquot of cDNA obtained by reverse transcription was amplified in a PCR reaction with GoTaq Green Master Mix (Promega, Milan, Italy). For each pair of primers ...
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bioRxiv - Microbiology 2023Quote: ... 5’-CGCCTTCGTTACAAGCATCG-3’; antisense, 5’-AAGAGCAAACGCATCTCCGA-3’), GAPDH (sense, 5’-ACCCACTCCTCCACCTTTGAC-3’; antisense, 5’-CTGTTGCTGTAGCCAAATTCGT-3’) using Green GoTaq master mix (Promega, Madison, WI) with C1000 Touch Thermal Cycler (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: Endogenous target sites and reporter target sites for arrayed editing were amplified by GoTaq G2 Hot Start Green Master Mix (Promega, 30 cycles) and were purified with Sera-Mag Select (Merck) ...
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bioRxiv - Microbiology 2024Quote: ... we confirmed that each primer set amplified single bands in a ligation-dependent manner by GoTaq Hot Start Green Master Mix (Promega, Madison, WI) (Figure S10) ...
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bioRxiv - Molecular Biology 2020Quote: ... qRT-PCR was executed in a total of 20μl reaction volume which includes 2 X Mix SYBR green I (10μl; Promega Corporation, Madison, WI, USA), primer (0.25μl ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 μl of Master Mix (Promega, Madison, WI, USA), 3.94 μl of ddH2O ...
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bioRxiv - Microbiology 2019Quote: ... and analysed by PCR with GoTaq Master Mix (Promega) using the following conditions ...
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bioRxiv - Microbiology 2020Quote: ... a PCR reaction using the PCR Master Mix (Promega) and the primers rplU_Fwd and rplU_Rev (Table 2 ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNAs were mixed with the GoTaq master mix (Promega), and specific primer pairs for relevant genes (Supplementary Table 1 ...
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bioRxiv - Genetics 2022Quote: ... PCR was performed using GoTaq PCR Master Mix (Promega) with primers mo37 and ag489 ...
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bioRxiv - Microbiology 2023Quote: ... 40 μl TnT® Quick Master Mix (L1170, Promega), 1 μl Methionine (1 mM) ...
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bioRxiv - Genetics 2023Quote: ... 12.5 µL of Promega Master Mix (Cat# M7502 Promega), 6.5 µL of Molecular Biology Grade water (ref#46-000-CI Corning) ...
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bioRxiv - Genetics 2023Quote: ... 12.5 µL of Promega Master Mix (Cat# M7502 Promega), 6.5 µL of Molecular Biology Grade water (ref#46-000-CI Corning) ...
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bioRxiv - Genetics 2023Quote: ... 12.5 µL of Promega Master Mix (Cat# M7502 Promega), 6.5 µL of Molecular Biology Grade water (ref#46-000-CI Corning) ...
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bioRxiv - Microbiology 2024Quote: ... 12.5 µL GoTaq master mix (Promega, Madison, WI, USA) and 1 µL (10 ng µL−1 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCRs were performed in a total volume of 10µl (1µl DNA sample, 3µl Milli-Q water, 5µl GOTag Green Master Mix buffer (Promega, note with 1.5mM MgCl2 for 1x reaction), 0.5µl forward primer and of reverse primer each) ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by 85℃ heat inactivation for 1 hour and PCR-based genotyping (GoTaq Green Master Mix, Promega, and C1000 Touch Cycler, Bio-rad). Tcf12 [34] ...
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bioRxiv - Molecular Biology 2020Quote: The PCR mix included 10 µl Green GoTaq DNA polymerase mix (Promega, Madison, USA) with 200 nM of each primer and 10 ng genomic DNA ...