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Citations for Promega :
851 - 900 of 1564 citations for 2x SYBR Green qPCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... PCR amplification was performed in a 10- μL reaction mixture containing 5 μL of GoTaq Master Mix (Promega), 5 pmol FAM-labeled universal primer (5′ - FAM-gctacggactgacctcggac-3′) ...
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bioRxiv - Bioengineering 2020Quote: ... PCR reactions were carried out with 100 ng genomic DNA or cDNA in the GoTaq Master Mix (Promega) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... The reaction products served as templates for PCR with GoTaq ® G2 Hot Start Master Mix (Promega, USA), 300 nM forward primer ...
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bioRxiv - Microbiology 2023Quote: ... 2 ng of each purified epicPCR product was amplified using qacE_F (0.4 μM) and AP28_short (0.4 μM) primers in GoTaq polymerase master mix (Promega, United States) under the following thermocycling conditions ...
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bioRxiv - Cancer Biology 2023Quote: ... All PCR reactions were run using 2 µL of extracted DNA in GoTaq® PCR Master Mix (Promega) and the following cycling protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The target DNA was amplified in PCR reactions prepared with GoTaq Clear Master Mix (Promega, Cat. No. M7123) using 2 µL of the lysed samples per 25 µL PCR reaction ...
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bioRxiv - Neuroscience 2020Quote: One µl of bisulfite-converted DNA was subjected to PCR using the G2 Green Mix (Promega, Mannheim – Germany) with bisulfite-specific primers at temperatures and concentrations as indicated in Appendix 3 ...
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bioRxiv - Microbiology 2022Quote: ... Ten nanograms of template DNA was added to 5 µl Green GoTaq Reaction Mix Buffer (Promega, Madison, WI), 1.25 units GoTaq DNA Polymerase (Promega ...
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bioRxiv - Cell Biology 2019Quote: ... 500 ng RNA was used for reverse transcription using random primers and the GoScript master mix according to the manufacturer’s instructions (Promega). Quantitative real-time PCR was used to examine the expression levels of TRAP1 and HIF1A with GAPDH as the reference gene.
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bioRxiv - Cancer Biology 2020Quote: HPV16 integration into the IRF4 gene was confirmed by PCR using GoTaq Long PCR Master Mix kit (Promega U.S.), with 5’-AAGCATGTCAGACACGCAGA- 3’as a forward primer ...
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bioRxiv - Microbiology 2021Quote: ... 5 μL of DNA or 5 μL of cell lysate were mixed with 12.5 μL of GoTaq Colorless Master Mix (Promega), 1 μL of forward and reverse primer (5 μM ...
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bioRxiv - Plant Biology 2019Quote: ... PCR amplifications were carried out in a total reaction volume of 10μL containing 5 μL of 2xGoTaq master mix (Promega) and 2.5 μL of 4M betaine ...
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bioRxiv - Microbiology 2021Quote: ... Marker genes in isolated DNA were PCR-amplified using GoTaq Master Mix (Cat. No. M5133, Promega, Madison, WI, USA) as described in Caporaso et al.[24] PCR products were cleaned and normalized using the SequalPrepTM Normalization Plate Kit (Cat ...
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bioRxiv - Molecular Biology 2022Quote: Edited loci were amplified using locus-specific primer pairs described in Supplementary Table 1 using GoTaq master mix (Promega) and 200ng of genomic DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μg of pCMV-Sport- Polκ were incubated with 100 µL of TnT® Sp6 Quick Master Mix (Promega) supplemented with 4 μL of 1 mM methionine for 90 min at 30°C ...
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bioRxiv - Molecular Biology 2022Quote: ... GoTaq® qPCR and RT-qPCR Systems (Promega), and reverse transcription system (Promega) ...
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bioRxiv - Microbiology 2021Quote: ... Ten nanograms of template DNA was added to 5 μl Green GoTaq Reaction Mix Buffer (Promega, Madison, Wisconsin, USA), 1.25 units of GoTaq DNA Polymerase (Promega ...
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bioRxiv - Developmental Biology 2021Quote: ... Real time PCR was performed on an ABI Prism 7900 detection system using Gotaq master mix (Promega, Madison, WI, USA). Expression relative to the housekeeping gene TBP was calculated using the delta(d)Ct method and expressed as 2^(-dCT ...
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bioRxiv - Microbiology 2020Quote: ... Twenty nanograms of DNA were used as a model for 18 cycles of amplification of the V4 region of the 16S rRNA gene, using primers 515F and 806R (Caporaso, et al. 2012: 1621-4) and GoTaq Master Mix (Promega). PCR products were quantified with the Qschd dsDNA HS kit (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR was performed with 40 ng of cDNA for the cell and 1.6 ng of cDNA as the template and GoTaq™ master mix according to manufacturer instructions (Promega). The oligonucleotides were designed with PRIMER-Blast using the following parameters ...
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bioRxiv - Microbiology 2021Quote: 16S rRNA gene of each isolate was amplified from its genomic DNA using GoTaq G2 Hot Start Master Mix (Promega) and the purified PCR products were sequenced using BigDye Terminator v3.1 Cycle Sequencing Kit according to a previous report [15].
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bioRxiv - Evolutionary Biology 2021Quote: ... were then used to amplify a 443 bp region of the mitochondrial cox1 gene using GoTaq Hot Start Master Mix (Promega). Each 50 µL reaction contained 500 nM each primer and cycling conditions were as described above ...
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bioRxiv - Genetics 2020Quote: ... 1-2 μL of cDNA was used for 25 μL PCR reactions using the GoTaq Hot Start Master Mix (Promega). Cycling parameters consisted of an initial denaturation of 95°C for 2 min. ...
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bioRxiv - Microbiology 2024Quote: ... 2 µL of extracted DNA was added to an amplification mixture containing 1 µl (10 µM) of primers and 12.5 μl of PCR Master Mix Plus (Promega, USA). To each mixture ...
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bioRxiv - Neuroscience 2024Quote: ... Approximately 0.5µg of RNA from each sample were reverse-transcribed to become cDNA by mixing with the master-mix (200 u/µL M-MLV Reverse Transcriptase, Promega Cat ...
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bioRxiv - Microbiology 2024Quote: ... gonorrhoeae dedA gene and a kanamycin resistance cassette (Primer: kanR (kanamycin resistance) cassette F/R) were amplified using GoTaq master mix (Promega). The fragments were then assembled using NEBuilder HiFi DNA assembly master mix (NEB ...
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bioRxiv - Genomics 2022Quote: ... The efficiency and specificity of the designed primers was tested through PCR using the GoTaq Green Master kit (Promega, Madison, USA) with thermal cycling conditions of 2 min at 95 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... qPCR reactions were performed using GoTaq qPCR system (Promega).
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bioRxiv - Genomics 2024Quote: ... 1x SYBR Green I] and calculated from a standard dilution series of a PCR product (quantified with QuantiFluor® dsDNA System, Promega with a DeNovix DS-11 spectrometer ...
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bioRxiv - Cell Biology 2024Quote: Genotyping was performed by PCR in 20 µl volume reactions that included 1x GoTaq Green Mas-ter Mix (Promega #M7123), 1.1 mM MgCl2 ...
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bioRxiv - Biochemistry 2023Quote: ... 5 µL of 2x GoTaq (Promega, #A600A), 0,4 µL forward primer (0,5 µM) ...
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bioRxiv - Cell Biology 2021Quote: ... Real time quantitative PCR was performed in a CFX 96 BioRad CFX 96 Real-Time PCR detection instrument with GoTaq qRT-PCR master mix (Promega, A6002). The sequence of the primers used in this study are ...
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bioRxiv - Genomics 2021Quote: ... 23.5 μl PCR master mix (20 μl Buffer 5X, 2 μl dNTPs 10 μM, 1.5 μl GoTaq; Promega, cat. no. M3001), and 5 μl of Forward universal primer ...
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bioRxiv - Microbiology 2023Quote: ... Each PCR reaction with a total volume of 25 μL contained 12.5 μL of PCR master mix (Promega; Madison, WI, USA), 8.0 μL of nuclease free water ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR reactions were prepared in 25 μL final reaction volume containing 12,5 μL of GoTaq® G2 Hot Start Colorless Master Mix (Promega, USA), 0,25 μL (10 μM ...
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bioRxiv - Microbiology 2023Quote: ... Marker genes in isolated DNA were polymerase chain reaction (PCR)-amplified using GoTaq Master Mix (Cat. No. M5133, Promega, Madison, Wisconsin) and 515 F (5′-GTGCCAGCMGCCGCGGTAA-3′) ...
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bioRxiv - Molecular Biology 2024Quote: PCRs for amplification of chromosomal termini were performed on tailed DNA in 20 µL using GoTaq® Long PCR master Mix (Promega). PCR mix included 10 µL of GoTaq® Long PCR master Mix ...
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bioRxiv - Plant Biology 2020Quote: ... RT-qPCR analysis was conducted using the Roche LightCycler® 96 or the Bio-Rad CFX384 Touch™ with GoTaq SYBR green fluorescence detection (Promega). All reactions were performed in duplicate for at least two biological replicates (n = 11-14 ...
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bioRxiv - Cell Biology 2020Quote: ... before performing qPCR using the GoTAQ qPCR Mastermix (Promega, cat #A6001) according to the manufacturer’s instructions ...
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Dual inhibition of coronavirus Mpro and PLpro enzymes by phenothiazines and their antiviral activitybioRxiv - Microbiology 2023Quote: ... qPCR was performed using GoTaq 1-Step RT-qPCR (Promega, A6020) reaction ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 µl of the RT product (diluted 1:5) was used for semi-quantitative PCR or qPCR reactions with Promega PCR Mix (Promega, Madison, Wisconsin, USA) and SYBR Green PCR Mix (Applied Biosystems ...
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bioRxiv - Cancer Biology 2022Quote: ... An equal volume of 2x PLB buffer (Promega) was added to permeabilize the yeast cells ...
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bioRxiv - Neuroscience 2021Quote: RT-qPCR was conducted using one-step RT-qPCR kit (Promega, A6020), according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The GoTaq® qPCR and RT-qPCR Systems kit (Promega, Beijing, China) was used to quantify the transcript abundance on the ABI 7500 real-time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR reactions using the GoTaq 1-Step RT-qPCR kit (Promega) were set-up to 10 µl final reaction volumes according to the manufacturer’s instructions containing 250 nM of each receptors primer pairs and 10 ng of RNA ...
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bioRxiv - Neuroscience 2023Quote: ... qPCR was carried out using GoTaq qPCR Probe Kit (Promega, Cat #A6110) in a QuantStudio3 RT-PCR system (Applied Biosystems™ ...
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bioRxiv - Biochemistry 2023Quote: ... CellTox Green Dye (CellTox™ Green Cytotoxicity Assay, Promega), either 2.5 mM or 100 µM of P[5]a ...
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bioRxiv - Physiology 2021Quote: ... using GoTaq qPCR (Promega) following manufacturer’s protocols ...
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bioRxiv - Microbiology 2023Quote: ... GoTaq qPCR MasterMix (Promega) and the desired primer set ...
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bioRxiv - Evolutionary Biology 2023Quote: Target gene and AAEL002401(reference gene) expression was quantified by RT-qPCR using FAM- and HEX -labelled GoTaq® Probe qPCR and RT-qPCR assay (Promega), TaqMan universal PCR master mix (Promega ...