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Citations for Promega :
7101 - 7150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The supernatant without beads was digested with 150 ng trypsin (Trypsin Gold, Promega) in 1.5 µL 50 mM ammonium bicarbonate followed by incubation at 37°C for 5 hours ...
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bioRxiv - Immunology 2023Quote: ... and qPCR was performed with the Gotaq qPCR master mix (A6001, Promega) and the CFX96 Touch Real-Time PCR Detection System (Biorad) ...
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CXCL17 binds efficaciously to glycosaminoglycans with the potential to modulate chemokine signallingbioRxiv - Immunology 2023Quote: ... 5% CO2 in a humidified chamber after which cells traversing the membrane were assessed by CellTiterGlo® Luminescent Cell Viability Assay (Promega; Madison, WI) according to the manufacturers protocol using a TopCount® NXTTM Microplate Scintillation and Luminescence Counter ...
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bioRxiv - Immunology 2023Quote: ... goat anti-rabbit IgG conjugated to horseradish peroxidase (HRP) (Promega) was added and incubated for one h at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... and the reaction was revealed with a solution of NBT (nitroblue tetrazolium) and BCIP (5-bromo-4-chloro-3-indolyl-phosphate) (Promega, Madison, WI, USA).
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bioRxiv - Microbiology 2023Quote: ... Membranes were then incubated with an alkaline phosphatase-conjugated anti-mouse IgG (Promega, Madison, WI, USA) as the secondary antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... Incremental digestion by exoIII (Promega, M1811) was performed by diluting the linearized plasmids to a final concentration of 33 ng/ul before adding the exoIII amount to final 0.1U per nanogram of DNA (Qubit reading) ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative PCR (qPCR) was performed using Go-Taq qPCR Master Mix (Promega) with 2 µL of cDNA or ‘no RT’ sample in a 20 µL reaction volume using 100 or 200 nM final concentration qPCR primers ...
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bioRxiv - Molecular Biology 2023Quote: RNA was purified from cells using the Maxwell RSC simply RNA tissue extraction kit (Promega) and the concentration was determined using a NanoDrop spectrophotometer (Thermo-Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: A total of 10 µg RNA was taken from each sample for reverse transcription (RT) using GoScript (Promega) reverse transcriptase (5 µg for RT ...
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bioRxiv - Microbiology 2023Quote: ... and genomic DNA was isolated from overnight cultures (5 mL) using the Wizard Genomic DNA Purification Kit (Promega GmbH, Walldorf) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and then retrotranscribed into cDNA using M-MLV reverse transcriptase (Promega) and Oligo(dT)15 primers (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... and Oligo(dT)15 primers (Promega) according to manufacturer’s instructions in a ProFlex PCR machine (Applied Biosystems).
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bioRxiv - Neuroscience 2023Quote: ... plus helper plasmids pVSV-G (18 mg) and psPAX2 (27 mg) using FuGENE HD (Promega). DNA was incubated with 210 ml FuGENE HD in 4.5 ml Opti-MEM (Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... 24 h later cells were transfected with Fugene HD (Promega) and constructs containing dCas9-VPR and gRNA vectors targeting the conserved DAR region (500ng plasmid DNA in molar ratios sgRNA:dCas9-VPR) ...
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bioRxiv - Microbiology 2023Quote: The protein was digested overnight with 0.1 μg LysC (Promega) and 0.8 μg trypsin (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated with 10µM Coelenterazine-h (Promega) in the dark for 2 hours before recording luminescence in a plate reader (Optima FLUOStar ...
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bioRxiv - Molecular Biology 2023Quote: ... a PCR using GoTaq G2 polymerase (Promega) was performed to specifically amplify the wild-type target locus ...
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bioRxiv - Molecular Biology 2023Quote: ... a qPCR was performed using 40 ng digested genomic DNA as template with the GoTaq qPCR master mix (Promega) according to manufacturer’s instruction ...
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bioRxiv - Systems Biology 2023Quote: ... CellTiterGloⓇ luminescent assay (Promega #G75752) was used to approximate cell viability and generate a dose-response curve ...
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bioRxiv - Systems Biology 2023Quote: Methyltransferase activity of the NSP10-NSP16 complex and inhibitor screening was performed using the MTase-Glo assay (Cat. No. V7601, Promega) (Hsiao et al ...
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bioRxiv - Plant Biology 2023Quote: ... RNA was primed with oligo(dT)15 using M-MLV reverse transcriptase (Promega, https://france.promega.com). Relative transcript levels were determined with the LightCycler 480 SYBR Green I Master kit (Roche ...
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bioRxiv - Microbiology 2023Quote: ... Transcend Biotin-Lysyl-tRNA (Promega) was incorporated in reactions so that reaction products could be identified using western blotting as above ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was isolated with the Maxwell RSC viral total nucleic acid purification kit on a Maxwell RSC 48 instrument (Promega). Isolated ZIKV RNA was titrated by qRT-PCR using TaqMan Fast virus 1-step master mix (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... PCR fragments from the nested PCR were cloned into pGEM-T-easy vectors (Promega), and the indicated number of successful recombinant clones was randomly selected and sequenced using a 3500 Genetic Analyzer (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... Cell proliferation was assessed every 24 h by the MTS assay (CellTiter 96® AQueous One Solution Cell Proliferation, Promega). DMSO-treated cells were used as negative controls and the effects on cell proliferation were expressed as the percentage inhibition compared to the cells of the control group ...
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bioRxiv - Microbiology 2023Quote: ... cells were lysed with Bright-Glo reagent (Promega, Southampton, UK), and luciferase expression levels were assessed using a GloMax plate reader (Promega ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized using GoScript Reverse transcriptase (Promega, Madison, WI, USA), and real-time PCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were then digested with trypsin (Promega) - 1:100 enzyme-to- protein ratio at 37°C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... After the sequential digestion with LysC and trypsin (Promega, each protease to protein ratio 1:80), beads were retained with a magnetic rack and samples were filtered with Costar Spin-X spin filters (0·22 µm cut-off ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the NLuc-substrate furimazine (Promega, N157B) in measuring buffer (1:3,500 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Glycerol was quantified using the luminescence Glycerol-Glo assay (J3151, Promega), as per manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Cells were labelled with 1 μM HaloTag Janelia Fluor 646 (JF646) (Promega) in complete culture medium (without antibiotics ...
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bioRxiv - Physiology 2023Quote: ... for 45 minutes as per the protocol described elsewhere(Villanueva et al, 2020) These samples were then subjected to trypsin (cat no V5111 sequencing grade, Promega) digestion in an enzyme to substrate ratio of 1:20 (trypsin ...
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bioRxiv - Plant Biology 2023Quote: ... Ligation was performed using T4 ligase enzyme (Promega, Madison, WI, USA) and a molarity ratio of insert:vector equal to 3:1 for all the constructs ...
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bioRxiv - Plant Biology 2023Quote: ... 2μg of RNA per sample were treated with DNase RQ1 RNase-Free (Promega, Madison, WI, USA); and used for synthesis of cDNA with Super-Script IV enzyme (Invitrogen) ...
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bioRxiv - Systems Biology 2023Quote: ... 5 x 106 cells were electroporated with 1 μg of NanoLuc pNL3.1 (Promega, N1031) vector and 0.2 μg of control vector ...
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bioRxiv - Systems Biology 2023Quote: ... 2 mg of total RNA was used for cDNA synthesis with MMLV reverse transcriptase (Promega) in final 50 mL volume ...
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bioRxiv - Systems Biology 2023Quote: ... The biotinylated proteins bound to the beads were digested with sequencing-grade trypsin (Promega, Madison, WI, USA) in 100 mM ammonium bicarbonate (pH 8 ...
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bioRxiv - Systems Biology 2023Quote: ... Samples were digested overnight with 0.4ug Trypsin (Promega, #V5113) for a 1:100 enzyme to protein ratio ...
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bioRxiv - Systems Biology 2023Quote: ... The washed protein was then digested with trypsin (sequencing grade modified trypsin; Promega) in a mixture of 100 mM ammonium bicarbonate (pH 8 ...
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bioRxiv - Systems Biology 2023Quote: ... we used the Dual-Glo® luciferase assay kit (Promega) to measure the activities of both firefly and renilla luciferase in the cell lysate harvested from each well of the 24-well plate ...
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bioRxiv - Systems Biology 2023Quote: ... then proteins were digested with 1 μg of mass spectrometry grade Trypsin/Lys-C enzyme mix (Promega) in 50 μL of digestion buffer (100 mM ABC ...
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bioRxiv - Microbiology 2023Quote: ... Imaging was performed 3 min after intraperitoneal (i.p.) injection of 150 mg/kg D-Luciferin (Beetle Luciferin Potassium Salt, Promega) in the IVIS® Spectrum In Vivo Imaging System under 2.5% isoflurane inhalation anesthesia using 15 min exposure ...
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bioRxiv - Microbiology 2023Quote: ... the infected cells were lysed with a One-Glo luciferase assay system (Promega, Cat# E6130), a Bright-Glo luciferase assay system (Promega ...
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bioRxiv - Microbiology 2023Quote: ... genomic DNA was isolated from sporozoites using a genomic DNA purification kit (Promega) and genotyped using primers 1409/1400 (primers are listed in Table S3 ...
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bioRxiv - Microbiology 2023Quote: ... D-Luciferin (Steady-Glo Luciferase Assay System, Promega) was added to each well ...
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bioRxiv - Microbiology 2023Quote: ... 5 μM 11S and 2 μl furimazine solution (Promega Cat. # N1610) were added and the luminescence read at 1 min intervals for 10 min using the FLUOstar Omega using a gain value of 3,000 ...
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bioRxiv - Plant Biology 2023Quote: ... The fragments were T-cloned using the pGEM®-T Easy Vector System I (cat A1360, Promega) and plated on X-gal/IPTG plates ...
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bioRxiv - Plant Biology 2023Quote: ... 50 μl of leaf extract was mixed with 50 μl luciferase substrate (Promega, E1501) in a white-bottomed 96-well plate ...