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Citations for Promega :
7001 - 7050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 10 million cells were seeded in a 10cm cell culture dish and were transiently transfected with 17 μg of plasmid cDNA (pcDNA 3.4 TOPO) and 53 μl Fugene HD (Promega) in 10 ml of HEK293 media ...
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bioRxiv - Molecular Biology 2023Quote: ... Insulin and glucagon secretion were assayed using Lumit Insulin Immunoassay (Promega CS3037A01) and Lumit Glucagon Immunoassay (Promega W8022 ...
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bioRxiv - Molecular Biology 2023Quote: ... and Lumit Glucagon Immunoassay (Promega W8022) and measured using a TECAN Spark plate reader.
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bioRxiv - Molecular Biology 2023Quote: ... After 5 h the medium was supplemented with nutrients and cells were then transfected with the same double-stranded RNAs using FuGENE-HD Transfection Reagent (Promega, #E2311): the transfection mix containing 30 µl of serum-free medium M3 ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were harvested and both the firefly and Renilla luciferase activities were measured from the same sample using the Dual-Luciferase Reporter Assay System (Promega) according to manufacturer instructions on a NOVOstar microplate reader (BMG Labtech) ...
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bioRxiv - Molecular Biology 2023Quote: ... 500 ng of plasmid DNA encoding for SthCsm-complex and guide RNA was mixed with 1.5 μL of FuGENE HD reagent (Promega), incubated for 15 min ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 ng of plasmid encoding for SthCsm and 50 ng of plasmid encoding for a reporter (stop-GFP or GFP-stop-Luc) were mixed with 0.8 μL FuGENE HD reagent (Promega), incubated for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... and cells were lysed in 50 µL of the 1X Cell Culture Lysis Reagent (Promega). Lysates were used for luciferase assays (20 µL ...
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bioRxiv - Molecular Biology 2023Quote: ... and 50 ng of the plasmid encoding for the GFP-stop-Luc reporter were mixed with 1.1 μ FuGENE HD reagent (Promega), incubated for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... using the DeadEnd Fluorometric TUNEL System (G3250; Promega). Samples were then immunostained with insulin antibody (GTX39371 ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were rinsed using 80% EtOH and proteins were digested with 100 µL of trypsin in 50 mM TEAB pH 7.55 (Promega, protein:enzyme ratio 1:50 ...
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bioRxiv - Molecular Biology 2023Quote: ... Viability was quantified 24 hours after treatment using a luminescent system (CellTiter-Glo, Promega). In experiments using MEFs ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing-grade Trypsin was purchased from Promega (Madison, Wisconsin, USA). BCA reagent was obtained from ThermoFisher Scientific (Pierce Biotechnology ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were transfected with 4 µL FuGene HD (Promega), 1 µg sgRNA-Cas9 plasmid DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... 200 U/mL RNAsin (Promega), 1 complete Mini Protease Inhibitor Tablet (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... 250 μL of 50 mM TEAB was added to 20 μg/vial of Trypsin (Promega) for dissolution ...
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bioRxiv - Molecular Biology 2023Quote: RNA extractions were performed from cell pellets using the Maxwell RSC simplyRNA kit (Promega) using a Maxwell RSC Instrument (Promega).
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bioRxiv - Neuroscience 2023Quote: ... Proteins were precipitated with trichloroacetic acid and digested with Lys-C (Wako) and trypsin (Promega). The peptides were then analysed using an Ultimate 3000 nano-RSLC (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... pEGFP-N3 was from Clontech and pGEM-T Easy was from Promega (cat# A137A). New constructs were generated as described in Table S6 using routine molecular cloning techniques and enzymes from New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... Concentration of ChIP-nexus libraries was measured using the Quantus fluorometer (Promega) and fragment size distribution was checked on Agilent Bioanalyzer using the High Sensitivity DNA Assay ...
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bioRxiv - Molecular Biology 2023Quote: ... together with pCG-gag-pol and pCG-VSV-G (a gift from Dr. T. Yasui, National Institutes of Biomedical Innovation, Health and Nutrition) using FuGENE HD (E2311; Promega) for 4 h in Opti-MEM (31985-070 ...
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bioRxiv - Molecular Biology 2023Quote: ... pGEM®-T Easy Vector Systems and T4 DNA Ligase were from Promega (Madison, WI). Restriction enzymes and Q5® High-Fidelity DNA Polymerase were from New England Biolabs (Ipswich ...
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bioRxiv - Molecular Biology 2023Quote: ... Supernatant was collected and used for taking the luciferase reading using Dual Luciferase kit (Promega) as per manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: Transfected cells were harvested and lysed using 1X Passive Lysis Buffer (Promega). Cell lysates were centrifuged at 10,000 rpm for 10 minutes at 4℃ ...
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bioRxiv - Molecular Biology 2023Quote: ... Transfections of cells were performed using FuGENE® HD Transfection Reagent (Promega) according to the manufacturer’s instructions (Promega) ...
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bioRxiv - Neuroscience 2023Quote: ... according to the manufacturer’s protocol with the modification of on column DNase digestion using RQ1 RNase free DNase (Promega, Madison, WI) in the enzyme mix ...
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bioRxiv - Neuroscience 2023Quote: Intracellular ATP concentrations were measured in 38-45 independent wells per group from two unique culture preparations using the CellTiter-Glo 2.0 reagent (Promega Corp.; Madison, WI) as described (21) ...
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bioRxiv - Neuroscience 2023Quote: ... 0.5U/uL RNasin (Promega, N2615), 0.5U/uL SUPERase RNase inhibitor (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNA was reverse transcribed using random decamers and M-MLV reverse transcriptase (Promega)/Superscript II RNase H reverse transcriptase (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: Adult worms were lysed following standard protocols and PCRs were performed using GoTaq Green Master Mix (Promega, REF-M7123). Mutant alleles were distinguished from wild-type by imaging Restriction Fragment Length Polymorphisms (RFLP ...
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bioRxiv - Neuroscience 2023Quote: ... Both HEK293T cells and N2A cells were transfected with indicated plasmids via Fugene (Promega, E2311) as previously described46 ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing-grade trypsin/Lys-C mixture (Promega) was added at 1:50 protease to protein ratio before incubation at 37°C for 16 hours ...
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bioRxiv - Immunology 2023Quote: ... cDNA was synthesized with M-MLV reverse transcriptase Kit (Promega) and diluted 1/5 before further application ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed using the GoTaq 1-step RT-qPCR system (Promega). Knockdowns were verified using primers described in Table I ...
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bioRxiv - Microbiology 2023Quote: RT-qPCRs were performed with MLV SU or 2-LTR primers (Table 1) using a Power SYBR green PCR kit (Promega) and the QuantStudio 5 real-time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: ... The kan gene was then inserted between the upstream and downstream 05515-05525 flanking PCR fragments and simultaneously inserted into the pGEM-7Zf vector (Promega) in the BamHI and XhoI sites using the In-Fusion kit according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA (500 ng) was reverse transcribed using random primers (Promega) and AMV Reverse Transcriptase (Promega ...
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bioRxiv - Microbiology 2023Quote: ... Samples were harvested at specified timepoints with 1x passive lysis buffer before measurement of NLuc activity using the Nano-glo luciferase assay system (Promega) with the GloMax-96 luminometer (Promega).
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bioRxiv - Microbiology 2023Quote: ... The pCMV-Nanoluc (Promega) plasmid was used as an internal control to normalize transfection efficiency.
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bioRxiv - Microbiology 2023Quote: VHH ORFs were cloned in pCI mammalian expression vector (in Nhe1-Mlu1 restriction sites; Promega). The VHH16 ORF was also fused in frame with sequences encoding the 2A peptide of porcine teschovirus 1 and GFP and placed under the control of a constitutive promoter in a lentiviral vector – the 2A peptide allowing the co-translational cleavage between VHH16 and GFP ...
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bioRxiv - Genomics 2023Quote: ... using the Maxwell® 16 LEV plant RNA kit (Promega). The RNA concentration was measured by Nanodrop (Thermofisher) ...
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bioRxiv - Genomics 2023Quote: ... According to the manufacturer’s protocol (Promega), RNA was reverse transcribed to cDNA firstly with random hexamers (50 ng/µl ...
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bioRxiv - Genomics 2023Quote: ... and then RNA isolation was done by Maxwell® AS2000 (Promega) using the Maxwell® 16 LEV plant RNA kit (Promega) ...
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bioRxiv - Genomics 2023Quote: ... Genomic DNA was extracted using the Wizard SV genomic DNA purification kit (Promega). Virus titer and copy number of viral particles per cell were measured by qPCR as previously described [22].
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bioRxiv - Genomics 2023Quote: ... The IL experiment RNA isolation was conducted using the Maxwell® 16 LEV plant RNA kit (Promega) (35) ...
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bioRxiv - Genetics 2023Quote: ... we cloned the 300 bp region, rs35907548 at the middle (chr12:129,282,013-129,282,312, hg 19) locus into pGL4.26 (Promega) and co-transfected with pGL4.74 (internal control ...
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bioRxiv - Genetics 2023Quote: ... as template and cloned in pGL4.23 Firefly luciferase reporter vector (Promega, Wisconsin, Madison, United States). In addition ...
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bioRxiv - Genetics 2023Quote: ... Purified RNA was treated with DNase (Promega) at 37°C for 30 min ...
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bioRxiv - Genetics 2023Quote: ... 1μl of random primers (Promega), RNasin Plus RNase inhibitor (Promega) ...
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bioRxiv - Genetics 2023Quote: ... pGL4.10[Luc2] (#E6651 Promega, Southampton, UK) and PCR products were digested with KpnI and HindIII ...