Labshake search
Citations for Promega :
6101 - 6150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... Serially diluted inhibitor and the corresponding NanoBRET Tracer (Promega) at a concentration determined previously as the tracer KD,app were pipetted into white 384-well plates (Greiner #781207 ...
-
bioRxiv - Microbiology 2023Quote: ... cells were washed once in PBS and lysed in 35 μl 1x Luciferase Cell culture lysis buffer (E1531, Promega) for 15min at room temperature and centrifuged for 10min at 4°C ...
-
bioRxiv - Biochemistry 2023Quote: ... Purified DNA was ligated into a pGEM-T Easy pre-linearized vector (Promega, catalog no. A1360) and transformed into DH5α competent cells ...
-
bioRxiv - Biochemistry 2023Quote: ... Digest (Promega GmbH, Walldorf, Germany), and MassPREP E ...
-
bioRxiv - Biochemistry 2023Quote: ... cell death was measured using the CellTiter-Glo 2.0 (CTG) luminescent cell viability assay kit (Promega).
-
bioRxiv - Cell Biology 2023Quote: ... a 1839 bp region between two KpnI sites was amplified by PCR and subcloned into pGEM-T Easy (Promega, Madison, WI). This subclone was subjected to site-directed mutagenesis using the Q5 site-directed mutagenesis kit (New England Biolabs ...
-
bioRxiv - Cell Biology 2023Quote: ... Sequencing-grade porcine trypsin/Lys-C mix (Promega, Madison, WI) was added ...
-
bioRxiv - Neuroscience 2023Quote: ... animals were anesthetized with isoflurane inhalation and 10uls of 1% wheat germ agglutinin (WGA)-AF488 (Promega), diluted in 1%DMSO ...
-
bioRxiv - Cell Biology 2023Quote: ... Other parameters of ER Stress induced cell death were measured through immunoblotting or with the Caspase 3/7 Glo Assay Kit (Promega) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were digested by incubation with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin) for 12h at 37°C ...
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were alkylated using 15 mM chloroacetamide at 37° C for 30min and further digested using sequencing-grade modified trypsin (1/50 w/w, ratio trypsin/protein; Promega, USA) at 37°C for 12h ...
-
bioRxiv - Biophysics 2023Quote: RNA extraction was performed on PBS-prewashed cell pellets using ReliaPrepTM RNA cell Miniprep Systems according to the manufacturer’s instruction (Z6010, Promega). The concentration of extracted RNA was determined by NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer (ND-ONE-W ...
-
bioRxiv - Biophysics 2023Quote: ... and GloMax® Explorer Multimode Microplate Reader (GM3500, Promega).
-
bioRxiv - Cell Biology 2023Quote: ... RT-PCR was performed using GoTaq Green Master Mix (Promega, M7123). 2 µl of diluted cDNA was added to 12.5 µl GoTaq Green Master Mix and 0.4 µM of forward and reverse primers and nuclease-free water in a 25 µl reaction ...
-
bioRxiv - Cell Biology 2023Quote: ... Protein samples were digested overnight with 1ul of 0.1μg/ul LysC (Promega) and 2ul of 0.4μg/ul trypsin (Pierce ...
-
bioRxiv - Biochemistry 2023Quote: ... nLuc luciferase signal was measured by mixing 25 μL of Nano-Glo Luciferase Assay System (prepared 1:50 as recommended; Promega # N1120) with 25 μL of diluted reactions (diluted 1:5 in Glo Lysis Buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... purified using the PureYield Plasmid Miniprep or Midiprep Systems (Promega # A1222 and A2495), and validated by Sanger sequencing at The Ohio State University Comprehensive Cancer Center Genomics Shared Resource (OSUCCC GSR) ...
-
bioRxiv - Biochemistry 2023Quote: ... 25 μL diluted lysate was mixed with an equal volume of Renilla-Glo Luciferase Assay System (prepared 1:100 as recommended; Promega # E2710) for 10 min at room temperature.
-
bioRxiv - Biophysics 2023Quote: ... followed by polymerase chain reaction (PCR) with GoTaq® G2 Master Mix (M7822, Promega) on Biometra TRIO Touch Thermocycler (207072X ...
-
bioRxiv - Biophysics 2023Quote: ... we normalized the luminescent signal to the fluorescent signal obtained with CellTiter-Fluor® Cell Viability assay (G6082, Promega). The signals were detected with Spark® multimode microplate (Tecan ...
-
bioRxiv - Genomics 2023Quote: Luciferase assays were performed using the Nano-Glo Dual-Luciferase Reporter Assay System (Promega Cat#N1630) following the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2023Quote: ... PCR products were sub-cloned into the TAK101 vector (TOYOBO) and RACE product was sub-cloned into the pGEM-T Easy vector (Promega). The resulting plasmids were used for RNA probe synthesis with digoxigenin (DIG ...
-
bioRxiv - Genomics 2023Quote: The pNL1.1.CMV [Nluc/CMV] and pGL4.23 [luc2/minP] vectors were obtained from Promega. Luciferase elements were generated by selecting 467 bp of the nominated region and both the forward and reverse complement sequences were ordered as gBlocks from Integrated DNA Technologies (IDT) ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: Cardiomyocyte viability was measured as changes in cellular ATP levels using the CellTiter-Glo® 2.0 Cell Viability Assay (Promega). Cardiomyocytes cultured for 20 h were stimulated with CNP (1 µM) ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: Cell death was measured by the LDH-Glo™ Cytotoxicity Assay (Promega, Mannheim, Germany). Cardiomyocytes cultured for 20 h were stimulated with CNP (1 µM) ...
-
bioRxiv - Biochemistry 2023Quote: ... with 25 μL of diluted reactions (diluted 1:5 in Glo Lysis Buffer; Promega # E2661) and incubated at room temperature for 5 min ...
-
bioRxiv - Biochemistry 2023Quote: ... AAA start codon variants) was constructed by subcloning RLuc from pRL-SV40 (which was a kind gift from Aaron Goldstrohm; Promega # E2231) via PCR amplification to insert the N-terminal 3XFLAG tag ...
-
bioRxiv - Biochemistry 2023Quote: ... Total RNA was extracted from 400 μL of each fraction (spiked with 0.2 ng exogenous control FFLuc mRNA; Promega # L4561) by adding 600 μL TRIzol (Thermo Fisher # 15596018 ...
-
bioRxiv - Biochemistry 2023Quote: 10 μL in vitro nLuc mRNA translation reactions were performed in the dynamic linear range using 3 nM mRNA (31) in the Flexi Rabbit Reticulocyte Lysate (RRL) System (Promega # L4540) with final concentrations of reagents at 20% RRL ...
-
bioRxiv - Biophysics 2023Quote: ... Since Nano-Glo® Live Cell Assay (N2012, Promega) was not ratiometric and depended on the cell quantity ...
-
bioRxiv - Biophysics 2023Quote: ... NanoBiT® (Promega) allowed us to monitor protein-protein interactions between two Nav1.5-WT subunits in live cells using an aqueous ...
-
bioRxiv - Neuroscience 2023Quote: ... RNA was treated with DNase I (Promega) (1 μg DNase I/ 1 μg RNA ...
-
bioRxiv - Neuroscience 2023Quote: ... 0.5% Rnasin® Ribonuclease Inhibitors (Fisher/Promega, PRN2615), 1X Protease inhibitor ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins were subjected to in gel tryptic digest overnight at 37 °C with approximately 2 µg trypsin per 100 µl gel volume (Trypsin Gold, mass spectrometry grade, Promega). Peptides were eluted twice with 100 mM NH4HCO3 followed by an additional extraction with 50 mM NH4HCO3 in 50% acetonitrile ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were incubated in 1.5 mL per well of 20 nM TMR HaloTag ligand (Promega, G8251) in pre-warmed medium for 15 minutes ...
-
bioRxiv - Cell Biology 2023Quote: CellTiter-Glo (Promega, G9241) was performed in black opaque 96-well tissue culture plates (Corning ...
-
bioRxiv - Cell Biology 2023Quote: Subconfluent HaloTag-expressing cells were labeled with HaloTag-TMR (Promega, G8251), JFX-554 (Promega ...
-
bioRxiv - Cell Biology 2023Quote: ... The following antibodies were used: HaloTag mAB (Promega, G9211, 1:500), GAPDH (Cell Signaling 2118 ...
-
bioRxiv - Cell Biology 2023Quote: The MTS or CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega, G3580) was performed in transparent 96-well tissue culture plates (Corning ...
-
bioRxiv - Biophysics 2023Quote: ... were used to reverse transcribe a region of 23S rRNA using AMV reverse transcriptase (Promega). The region of cDNA containing the MS2 tag was amplified via PCR using primer pairs MS2_H98_quant_F and MS2_H98_quant_R or MS2_H25_quant_F and MS2_H25_quant_R ...
-
bioRxiv - Cancer Biology 2023Quote: ... Quantitative viability assays were performed by CellTiter-Glo® (Promega) or Alamar Blue® (Thermo Fisher ...
-
bioRxiv - Cancer Biology 2023Quote: ... The number of viable cells surviving drug treatment were measured with CellTiter-Glo (Promega #G7571) 24 hours after the last drug treatment per the manufacturer’s protocol using a BioTek Synergy H5 plate reader ...
-
bioRxiv - Molecular Biology 2023Quote: ... 10μl/ml rRNasin (Promega), 10μl/ml Superasin (Applied Biosystems) ...
-
bioRxiv - Zoology 2023Quote: ... 1 μl 10mM dNTPs (Promega), 2.5 μl of each primer at 10 μM ...
-
bioRxiv - Zoology 2023Quote: ... 0.25 μl Taq (Gotaq flexi, Promega), 1 μl template ...
-
bioRxiv - Zoology 2023Quote: ... and 3% 20 mg/ml Proteinase K (Promega). This digestion eliminates cellular material while leaving the spongin network intact ...
-
bioRxiv - Zoology 2023Quote: ... Skeletal architecture was examined by hand cutting tissue sections and digesting them with a mixture of 97% Nuclei Lysis Solution (Promega; from the Wizard DNA isolation kit) and 3% 20 mg/ml Proteinase K (Promega) ...
-
bioRxiv - Zoology 2023Quote: ... 10 μl 5x PCR buffer (Gotaq flexi, Promega), 8 μl MgCl ...
-
bioRxiv - Systems Biology 2023Quote: ... 10 µl of MTase-Glo Detection Solution (Promega) was added ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... The ReverTra Ace-cDNA synthesis kit (Promega, USA) was used to synthesize cDNA from 2 μg of total RNA ...