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Citations for Promega :
6001 - 6050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... 100 µL of luciferase substrate solution (Promega) was added to each well and luminescence was measured using a Spectramax L luminometer (Molecular Devices).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Relative light units (RLUs) were measured using a luminometer (Promega; GloMax). Triplicate data was analyzed in Prism Graphpad to generate IC50 values.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and the cells were washed with PBS and lysed with passive lysis buffer (Promega) for 20 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The plates were read within 30 min on a GloMax Discover luminometer (Promega) equipped with 450 nm BP filter (donor ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... at a ratio of 1:166 to Opti-MEM media in combination with extracellular NLuc Inhibitor (Promega) diluted 1:500 (10 μL of 30 mM stock per 5 mL Opti-MEM plus substrate ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... A total of 5 μL per well of 20x-NanoBRET Tracer K10 (Promega) at 10 μM for CSNK2A1 or 5 μM for CSNK2A2 in Tracer Dilution Buffer (Promega N291B ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... FuGENE HD (Promega) was added at 30 µl/mL to form a lipid:DNA complex ...
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bioRxiv - Neuroscience 2023Quote: ... digested using 100 ng/μL sequencing grade trypsin (Promega) for 1 hr at 47 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... at 10 μM for CSNK2A1 or 5 μM for CSNK2A2 in Tracer Dilution Buffer (Promega N291B) was added to all wells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... NanoBRET NanoGlo substrate (Promega) at a ratio of 1:166 to Opti-MEM media in combination with extracellular NLuc Inhibitor (Promega ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... consisting of 9 µg/mL of carrier DNA (Promega) and 1 µg/mL of CSNK2A-NLuc fusion DNA in Opti-MEM without serum (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... In vitro luciferase activity was measured using a Lumat model LB luminometer (Promega) and the Luciferase Reporter Gene Assay according to manufacturer’s instructions (Roche) ...
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bioRxiv - Cancer Biology 2023Quote: ... The compounds NanoBRET Nano-Glo Substrate (Promega #N1571) and HaloTag NanoBRET 618 Ligand (Promega #G9801 ...
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bioRxiv - Cell Biology 2023Quote: ... Transfection was performed in antibiotic free medium using FuGENE® HD transfection reagent (Promega Corporation, Durham, NC, USA) following the manufacturer’s instructions with a final plas-mid amount of ∼100-200 ng per well ...
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bioRxiv - Neuroscience 2023Quote: ... animals were anesthetized with isoflurane inhalation and 10uls of 1% wheat germ agglutinin (WGA)-AF488 (Promega), diluted in 1%DMSO ...
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bioRxiv - Cell Biology 2023Quote: ... Other parameters of ER Stress induced cell death were measured through immunoblotting or with the Caspase 3/7 Glo Assay Kit (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... by a terminal Deoxynucleotidyl transferase (Promega, M1875) with a specificity of 2.5 x 106 cpm/ml (C-Fos ...
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bioRxiv - Cancer Biology 2023Quote: ... The size of the amplified cDNA was selected using ProNex beads (Promega, Madison, WI) under standard conditions ...
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bioRxiv - Genetics 2023Quote: ... from HEK293A cells transfected with CROPseq-EFS-SpCas9-P2A-EGFP DNMT3B sgRNA plasmid using Fugene HD (Promega). PCR fragment for SURVEYOR assay was amplified using Q5 high-fidelity polymerase using the primers F ...
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bioRxiv - Genetics 2023Quote: ... The PCR product was cloned into pGEM-T plasmid (Promega) and sequenced ...
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bioRxiv - Genomics 2023Quote: ... and transfected (Fugene 6, Promega # E269A) with pBABE-puro SV40 LT plasmid (Addgene # 13970 ...
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bioRxiv - Cell Biology 2023Quote: ... a 1839 bp region between two KpnI sites was amplified by PCR and subcloned into pGEM-T Easy (Promega, Madison, WI). This subclone was subjected to site-directed mutagenesis using the Q5 site-directed mutagenesis kit (New England Biolabs ...
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bioRxiv - Cancer Biology 2023Quote: ... and HaloTag NanoBRET 618 Ligand (Promega #G9801) were used in NanoBRET assays ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR was used to amplify the coding regions of the steroid receptor genes and to linearize the expression plasmids pHTN HaloTag CMV Neo or pFLN-1 NanoLuc (Promega #N1811 ...
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bioRxiv - Cell Biology 2023Quote: ... using fuGENE HD™ transfection reagent (Promega, Cat. no. E2312) following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... peptides were eluted by on-bead trypsin (Promega) digest and acidified for MS analysis ...
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bioRxiv - Microbiology 2023Quote: ... measured on a plate reader (GloMax® Navigator Microplate Luminometer, Promega). For proteins expression analysis ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was cleared by centrifugation at 15,000 g for 15 min at 4°C and 45 μL of the supernatant were mixed with an equal volume of the NanoLuc Reagent (Promega N1110) prepared according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... For transient expression experiments each well was transfected with 100 ng of DNA using 1:3 ratio of FUGENE HD (#E2311, Promega). Twenty-four hours after transfection or doxycycline stimulation ...
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bioRxiv - Molecular Biology 2023Quote: Plasmid-based donor repair templates for CRISPR were prepared in pGEM-T Easy vector (Promega). Homology arms (each around 800-1000 bp ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing-grade porcine trypsin/Lys-C mix (Promega, Madison, WI) was added ...
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bioRxiv - Physiology 2023Quote: ... with added protease inhibitors (Promega, cat# G6521; Madison, WI, USA). Samples were centrifuged at 1,500 g for 10 minutes at 4°C ...
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bioRxiv - Physiology 2023Quote: ... 50 mM spermidine with added protease inhibitors (Promega, cat# G6521). Samples were then homogenized using tight-fitting pestles and stored at −80°C until shipment on dry ice to Seer ...
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bioRxiv - Plant Biology 2023Quote: ... we used a luciferase-based GTPase assay (GTPase-GloTM Assay Kit by Promega). The assay was carried out as per manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... Purified RNA was reverse-transcribed into cDNAs with GoScript Reverse Transcription System (Promega) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2023Quote: ... 1µl of reaction was added to 25µl of luciferase assay reagent (Promega) and luminescence activity was measured in each well.
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bioRxiv - Immunology 2023Quote: ... Remaining tumor cells were lysed using passive lysis buffer (Promega) and bioluminescence activity of each well was measured using D-luciferin (Luciferase Assay System ...
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bioRxiv - Immunology 2023Quote: Total RNA was isolated using the Maxwell RSC simplyRNA Cells Kit (Promega) and reverse-transcribed to cDNA with Transcriptor First Strand cDNA Synthesis Kit (Roche ...
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bioRxiv - Immunology 2023Quote: ... Luciferase expression in cells was measured by lysing the cells in Passive Lysis Buffer and incubating the lysis supernatant with the Luciferase Assay System (Promega, Madison, WI, USA). Luciferase activity was read on an Enspire Multimode Plate Reader (Perkin Elmer) ...
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bioRxiv - Immunology 2023Quote: ... The cells were lysed using Bright-Glo (Promega) and Luciferase expression was measured using the Enspire Multimode Plate Reader.
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bioRxiv - Microbiology 2023Quote: ... using Pfu DNA Polymerase from Promega. The following oligonucleotides were used to introduce the single R57D mutation ...
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bioRxiv - Plant Biology 2023Quote: ... and treated with an RNase-free DNase (Promega, USA) to remove any genomic DNA contamination ...
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bioRxiv - Plant Biology 2023Quote: ... Protoplasts were resuspended in 80 µL passive lysis buffer (Promega) on a rotary shaker for 30 min at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Samples were digested with 3µg of trypsin (Promega, Madison, WI, USA) at 47°C for 2 h ...
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bioRxiv - Microbiology 2023Quote: ... The concentrated phages were next treated for 30 min at 37°C with 10 µl of DNAse (Promega) and 2.5 µl of RNAse (Macherey-Nagel ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega).
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bioRxiv - Plant Biology 2023Quote: ... LUC images were taken 2-3 days after infiltration by applying 1 mM Beetle luciferin (Promega) on adaxial leaf surface.
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bioRxiv - Genomics 2023Quote: ... 1 μl of RNasin Plus RNase Inhibitor (catalog num. N2611, Promega), 4 μl of nuclease-free water (UltraPure DNase/RNase-Free Distilled Water ...
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bioRxiv - Microbiology 2023Quote: ... D-luciferin potassium salt (Promega) stock solution was prepared in sterile PBS at 33.33 mg/mL and stored in a -20°C freezer ...
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bioRxiv - Cell Biology 2023Quote: Glucose uptake was performed by using Glucose Uptake-Glo from Promega. Briefly ...