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Citations for Promega :
5501 - 5550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2023Quote: ... Their DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega) and was paired-end sequenced (2 × 150 bp ...
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bioRxiv - Pathology 2023Quote: ... 0.08 µL of GoTaq2 and 4 µL of its 5x corresponding buffer (Promega), and 5 µL of boiled calibrated bacterial suspensions ...
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bioRxiv - Microbiology 2023Quote: ... Two aliquots of 100 µL were transferred into new tubes and digested either with a sequencing-grade trypsin (Promega, Madison, WI, USA) or chymotrypsin (Promega ...
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bioRxiv - Microbiology 2023Quote: ... or chymotrypsin (Promega, Madison, WI, USA) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Cells were pelleted (2000 g x 5 min) and subjected to genomic DNA (gDNA) extraction using the Wizard Genomic DNA Purification kit (Promega), according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... higginsianum genomic DNA used as PCR templates were isolated by Maxwell RSC Plant DNA Kit with Maxwell RSC 48 instrument (Promega) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Samples were diluted in 50 mM ammonium bicarbonate and normalized to 50 μg protein for overnight LysC/trypsin digestion (Promega, protein:enzyme ratio, 50:1). Trifluoroacetic acid (TFA ...
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bioRxiv - Microbiology 2023Quote: ... genomic DNA was extracted using the Wizard® Genomic DNA Purification Kit (Promega, Madison, WI, USA) and the libraries were prepared using the Rapid Barcoding Sequencing kit (SQK-RBK004 ...
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bioRxiv - Neuroscience 2023Quote: ... pcDNA3-GloSensor (Promega, WI, USA) and pcDNA3-D2R-expression vector (generated with RT-PCR of rat brain mRNA library) ...
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bioRxiv - Neuroscience 2023Quote: ... Mass Spec Grade (Promega) was made up at 100ug/mL in 50mM TEAB ...
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bioRxiv - Neuroscience 2023Quote: ... Mass Spec Grade (Promega) was made up at 100ug/mL in 50mM TEAB ...
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bioRxiv - Microbiology 2023Quote: ... cell culture supernatants from the same infections were collected and used to measure Nluc expression using the Nano-Glo luciferase substrate (Promega) and the microplate reader (BioTek Synergy) ...
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bioRxiv - Microbiology 2023Quote: ... RNA samples were treated with RQ1 DNase (Promega) and repurified with the RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Microbiology 2023Quote: ... 1% parasitemia and lysed in 1x Nano-Glo Lysis Buffer (Promega, USA), before being added to a white 96-well plate in triplicate ...
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bioRxiv - Microbiology 2023Quote: Lentiviral particles were produced by transfection of HEK293T cells in T150 flasks using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... GloSensor plasmid was acquired from Promega (WI, USA). Mammalian codon-optimized CRY2PHR and CIBN sequences were synthesized by Genscript and subcloned into the appropriate vectors ...
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bioRxiv - Genetics 2023Quote: ... and 1 unit of GoTaq G2 Flexi DNA Polymerase (Promega, USA). DNA concentrations was rarely quantified as amplification was often successful across a range of values (e.g ...
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bioRxiv - Genetics 2023Quote: ... 3μL of 5X Colorless GoTaq Flexi buffer (Promega, USA), 2.0 mM MgCl2 ...
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bioRxiv - Genomics 2023Quote: ... cell survival was measured using the CellTiter-Glo Luminescent Cell Viability Assay (Promega) according to the manufacturer’s protocol.
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bioRxiv - Genomics 2023Quote: ... cells were incubated with DSPE liposomes at concentrations of 0-5mg/ml in DMEM containing the viability dye from the RealTime-Glo MT Cell Viability Assay (cat: G9712, Promega). After incubation with liposomes for 4h ...
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bioRxiv - Microbiology 2023Quote: ... 1 μg trypsin (Sequencing grade Modified Trypsin, Promega) was added to the sample ...
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bioRxiv - Microbiology 2023Quote: ... 5X reporter lysis buffer (Promega, Southampton, UK) was diluted in sterile ddH2O and 100μL was added per well ...
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bioRxiv - Cell Biology 2023Quote: 35S-radiolabeled Tom40 precursor proteins were synthesized in vitro using the TNT Quick Coupled Transcription/Translation kit (Promega). Transcripts for MCO6 encoding 3 additional N-terminal methionine residues for radiolabelling of the Mco6 protein which lacks internal methionine residues ...
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bioRxiv - Cell Biology 2023Quote: ... and digested with 0.5 μg of trypsin (Sequencing Grade, Promega, or Pierce) overnight in 50mM HEPES (pH 8.0 ...
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bioRxiv - Molecular Biology 2023Quote: Cardiomyocytes isolated from WT and Rpl3l-/- hearts were diluted to same concentrations and the cell suspensions were mixed with the same volume of CellTiter-Glo (Promega, G7570), as instructed by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: The SV total RNA isolation system kit (Promega) was employed to isolate RNA from cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was measured using CellTiter-Glo 2.0 Assay (Promega G9243) to find IC20 concentrations.
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bioRxiv - Developmental Biology 2023Quote: ... the cells were transfected using FuGENE 6 transfection reagent (Promega). Transfection mixture was prepared in serum-reduced OptiMEM medium (Thermo ...
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bioRxiv - Developmental Biology 2023Quote: ... dNTP mix (0.3 mM each, Promega) with 9-10 cycles of PCR ...
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bioRxiv - Developmental Biology 2023Quote: ... Dried gel samples were digested overnight with mass spectrometry grade trypsin (Trypsin Gold, Promega, Madison, WI, USA) at 5ng/μl in 50 mM NH4HCO3 digest buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... and genomic DNA (gDNA) was extracted for genotyping using the Wizard® Genomic DNA Purification Kit (Promega, #A1120). A 781bp fragment around the expected mutation site was amplified by PCR ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and a Dual Injector System for GloMax-Multi Detection System (Promega) luminometer were used to measure luciferase activity ...
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bioRxiv - Evolutionary Biology 2023Quote: ... aliquots of a single preparation of the basal firefly vector pGL4.23[luc2/minP] (Promega, Genbank Accession # DQ904455.1) were assayed on each experimental day ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Primers introducing homology arms for Gibson Assembly were used to clone candidate sequences into either the GFP reporter vector pT2HE (Howes, Summers and Kingsley, 2017) and/or the luciferase reporter vector pGL4.23[luc2/minP] (Promega, Genbank Accession # DQ904455.1) using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs catalog # E2621) ...
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bioRxiv - Cell Biology 2023Quote: ... FuGENE HD (Promega) transfection reagent was used to transfect RAW cells at a 3.5:1 ratio (using 1.75 µL FuGENE HD and 0.5 µg DNA per well) ...
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bioRxiv - Cell Biology 2023Quote: ... was obtained through transfecting HEK293T cells with these plasmids in combination with a second generation CMV lentiviral packaging system and FuGENE transfection agent (E2311, Promega).
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bioRxiv - Cell Biology 2023Quote: ... For in vitro import assay WT and MLS-TFEB cDNA were cloned into the pGEM-4 vector and transcribed in vitro using the TNT Quick coupled translation system (L1170, Promega), by incubating 200 ng of DNA with 7 μL of TNT-T7 reticulocyte lysate mix and 0.5 µL of 35S-labelled methionine (NEG709A500UC ...
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bioRxiv - Cell Biology 2023Quote: ... was synthesized in vitro using the TNT-coupled transcription-translation system (Promega, Madison, WI) using DNA templates pcDNA3.1– 2xHA-PXF-1 and pcDNA3.1–2xHA-PXF-1(K191/P195/P198->AAA) ...
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bioRxiv - Immunology 2023Quote: ... DNA was isolated from these samples using a DNA cleanup kit (Promega Wizard).
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bioRxiv - Immunology 2023Quote: ... oligo dT and dNTPs (Promega). Transcripts were quantified by real time PCR on a 480 LightCycler instrument (Roche) ...
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bioRxiv - Immunology 2023Quote: ... Blots were developed using chemiluminescent substrate (Promega) and X-ray film.
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bioRxiv - Pathology 2023Quote: ... RNA (1 µg) was reverse transcribed using GoScript Reverse Transcription System (A5001, Promega, Madison, WI). cDNA was measured by quantitative PCR with FastStart Universal SYBR Green Master (Rox ...
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bioRxiv - Microbiology 2023Quote: ... and the pCI-neo mammalian expression vector (Promega, WI, USA) was used as the backbone vector ...
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bioRxiv - Microbiology 2023Quote: ... the infected cells were lysed with a Bright-Glo luciferase assay system (Promega, Cat# E2650) and the intracellular luciferase activity was measured by a Synergy H1 microplate reader (BioTek ...
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bioRxiv - Microbiology 2023Quote: ... 2 μg of RNA was used for cDNA synthesis by Go Script TM Reverse Transcription System (Promega, Madison, USA). Then qRT-PCR was performed using Maxima SYBR Green/ROX qPCR master mix (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Plates were stored at -20°C before adding 100 μl Steady Glo substrate (Promega) to perform a luciferase assay as described earlier ...
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bioRxiv - Microbiology 2023Quote: ... GoTaq Flexi DNA polymerase (Promega, Wisconsin, USA) was used as per manufacturer’s instructions and 10 µmol of the primers Imp4_screen_F (5′-CCAGGACACACTCCAGATAACC-3′ ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were then digested overnight with 2 µg of lysyl endopeptidase (Wako) at room temperature and further digested overnight with 2 µg trypsin (Promega) at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... 12 μl Viafect Transfection Reagent (Promega) and MEM without antibiotics and FCS to a final volume of 300 μl ...
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bioRxiv - Microbiology 2023Quote: ... DNase (Promega, Madison, USA) treatment and control PCR ...