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Citations for BioLegend :
8851 - 8900 of 8901 citations for DNAM 1 Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... ASC was stained using a primary antibody anti-ASC (polyclonal anti-PYD ASC, Santa Cruz or monoclonal anti-CARD ASC, Biolegend; 1:1000 dilution) or anti-Luciferase (Abcam ...
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bioRxiv - Microbiology 2019Quote: ... Cells were permeabilized with ice-cold methanol at −20°C for at least 20 min and incubated with anti-Pol II antibody (Biolegend, 8WG16 at 1:200), anti-PABPC antibody (Abcam ab21060 at 1:200 for mouse cells and Santa Cruz sc32318 at 1:25 for human cells ...
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bioRxiv - Cell Biology 2021Quote: ... and the cell pellet was resuspended in 1 volume (V) CnT-PR-MSC-XF-HC before adding 3V of 1x Red Blood Cell Lysis Buffer (Biolegend, San Diego, United States). After 10 minutes of incubation at room temperature another 10V of CnT-PR-MSC-XF-HC were added and the cell suspension was centrifuged ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was discarded and the cell pellets were resuspended in PBS containing Zombie Violet dye (Biolegend; 1:200 in PBS; 100μl per well) and incubated at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... at a dilution of 1:200 for 30 min at room temperature followed by incubation with horseradish peroxidase streptavidin at a dilution of 1:125 (405210, BioLegend, San Diego, CA, USA) for 30 min at room temperature ...
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bioRxiv - Immunology 2020Quote: ... LNs were harvested and digested as below (preparation of single cell suspensions) and stained with CD45 brilliant violet 510 (Biolegend clone 30F11, 1:300), PDPN APC (Biolegend clone 8.1.1 ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissue was incubated in primary antibody overnight at 4°C in a moist chamber diluted in blocking buffer with the following dilutions: Anti-Tubulin β3 (BioLegend, clone TUJ1, 1:500); Anti-OMP (Santa Cruz ...
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bioRxiv - Cancer Biology 2022Quote: ... the antibody or IgG1 excess was rinsed and pellets were resuspended in APC-conjugated Annexin V at 1:20 in Annexin V buffer solution plus Zombie Violet dye at 1:400 (all antibodies and probes are from Biolegend, San Diego, CA, USA). Samples were transferred into FACS tubes and incubated for 20 minutes at room temperature protected from light prior to their analysis by FACS Canto II (BD ...
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bioRxiv - Immunology 2022Quote: Antibody staining was done in the dark for 30 minutes at 4°C in 30 μL of FACS buffer with antibodies and anti-CD16/32 (TruStain FcX™ PLUS; BioLegend, #156604; 1:100). Acquisition was done on a BD FACSCanto™ II or BD LSRFortessa (both BD Biosciences) ...
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bioRxiv - Microbiology 2022Quote: ... To measure the cytokines/chemokines present in the SN we chose the LEGENDplex™ bead-based immunoassay and quantified the following cytokines/chemokines from the panels COVID-19 Cytokine Storm 1 (CCL2, CCL5, CXCL10, IFN-γ, IL-10, IL-6; Biolegend cat. No. 741088) and Human Neurodegeneration Biomarkers 1 (NFL ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: BALF and post-digested lungs were centrifuged at 1500 rpm before resuspension in 100 μL of a 1:100 dilution of Zombie Yellow™ dye (Biolegend, San Diego, USA). After staining ...
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bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used in this study: anti-tubulin beta-3 conjugated to Alexa Fluor 488 (BioLegend; AB_2562669; mouse IgG2a, 1:200), Alexa Fluor 555 (BD PharMingen ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were permeabilized with 0.1% Triton X-100 in PBS (5 min at RT) and immunolabeled with anti-HA (Biolegend, 901501; 1:500 in PBS) for 1 hr at RT ...
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bioRxiv - Cancer Biology 2023Quote: Other conjugated antibodies used in this study include the following: Alexa Fluor® 647 anti-Tubulin Beta 3 (1:1000, Biolegend, #657406, clone AA10), Alexa Fluor® 488 anti-Vimentin (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... and stained overnight at 4°C in blocking solution with DAPI and the following antibodies and dilutions: Alexa Fluor® 488 anti-Vimentin (1:500, Biolegend, #677809, clone O91D3), anti-Ki67 (1:300 ...
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bioRxiv - Immunology 2022Quote: ... 4 °C) cells were stained with the following conjugated antibodies for 20 min at RT in the dark: CD3-APC (Biolegend, clone REA613, 1:200), CD11b-APC-Cy7 (Biolegend ...
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bioRxiv - Neuroscience 2023Quote: ... were blocked with 5% BSA and 5% goat serum in PBST (PBS/0.25% Triton X-100) for 30 min and then incubated with monoclonal 6E10 antibody (BioLegend, cat# 803001, 1:1000 dilution) overnight at 4 °C ...
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bioRxiv - Bioengineering 2023Quote: ... the slices were transferred into 30% sucrose in water for >24 hours and then sub-sectioned on a sliding microtome to 15-30 μm for immunostaining using the following antibodies: mouse anti-HA (Biolegend catalog #901513, 1:1000) and rabbit anti-NeuN (Millipore catalog #ABN78 ...
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bioRxiv - Immunology 2023Quote: ... pancreatic islets were harvested from four 12-week-old female NOD mice and labelled individually with anti-TCRβ (BioLegend, allophycocyanin, H57-597, 1:300), anti-CD4 (BioLegend ...
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bioRxiv - Biophysics 2023Quote: ... were isolated from buffy coats by density gradient centrifugation (Lymphoprep, StemCell ref: 07851) and activated on plates coated with anti-CD3 (1 mg/ml, Biolegend, ref: 317347, clone: OKT3) and anti-CD28 (1 mg/ml ...
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bioRxiv - Biochemistry 2023Quote: ... and 0.2 g/L NaN3] for 15 min after which the grids were incubated on a 20 μl drop of primary antibodies—1:10 dilution of 6E10 (Biolegend, San Diego, CA, USA) and anti-αSyn MJFR1 (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were stained with unconjugated rat anti-mouse lineage-specific antibodies (Ter-119, Mac1, Gr-1, B220, CD5, CD3, CD4, CD8, and CD127, Biolegend, San Diego, CA, USA), followed by incubating with Dynabeads anti-rat IgG (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... or the following antibody panel where the 488 and 555 channel markers were swapped: Alexa Fluor® 488 anti-Nestin (1:150, Biolegend, #656812, clone 10C2), Alexa Fluor® 555 anti-S100 beta (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... the FOXP3/Transcription Factor Staining Buffer kit (eBioscences – 00-5523-00) was used along with the following anti-mouse antibodies: BV605-conjugated anti-CD4 (Biolegend, RM4-5; 1/700), BV650-conjugated anti-B220 (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... BV-421), CD62L (clone: MEL-14, PE), PD-1 (clone: 29F.1A12, PE-Cy7), CD107a (clone: 1D4B, APC) from BioLegend (San Diego, CA, USA), and NKG2D (clone ...
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bioRxiv - Microbiology 2023Quote: ... washed once with PBS and stained for 30 min at RT in the dark with anti-CD4 antibody (PerCP-Cy5.5, Biolegend #317428, 1:50 in PBS) and eBioscience Fixable viability dye 780 (ThermoFisher Scientific #65-0865-14 ...
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bioRxiv - Cell Biology 2023Quote: ... Antibodies were combined in different panels comprising of an appropriate selection from the following: a 1:400 dilution of Alexa Fluor™ 750 anti-murine CD8 (BioLegend, Cat. No. 100766), a 1:400 dilution of Brilliant Violet™ 605 anti-murine CD4 (BioLegend ...
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bioRxiv - Microbiology 2024Quote: ... 800 μl of crosslinked chromatin was incubated with 25 μl Protein A Sepharose slurry (50%) in TBS and 1 μl of antibody against RNAP β (Biolegend, #663003, clone: NT63) for 90 minutes at room temperature while rotating ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were fixed with 2% neutral-buffered formalin diluted in FACS buffer following addition of Zombie Yellow fixable viability dye (BioLegend #423103, 1:200 in PBS).
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bioRxiv - Immunology 2021Quote: ... and stained by intracellular staining for 30 min at 4 °C using a cocktail of the following antibodies: IFN-γ PE (Biolegend, Clone W18272D,1:500), TNF Percp-Cy5.5(Biolegend ...
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bioRxiv - Developmental Biology 2021Quote: ... the cells were pre-incubated for 20 minutes at 4°C in the dark with unlabelled rat anti-mouse CD16/32 (BioLegend – 101320, 1 μg/million cells), then for 1 hour at 4°C in the dark with a 1:1 mix of rat anti-mouse CD41 (labelled with BV421 ...
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bioRxiv - Developmental Biology 2021Quote: ... Single cell suspensions were pre-incubated for 20 minutes at 4°C in the dark with unlabelled rat anti-mouse CD16/32 (BioLegend – 101320, 1 μg/million cells), then stained for one hour at 4°C in the dark with a mix of rat anti-mouse CD117/c-kit (labelled with PE ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then incubated with the following primary antibodies diluted in the incubation solution overnight: chicken anti-neurofilament NF-M (1:1000) (#822701, BioLegend, USA, previously #PCK-593P, Covance), rat anti-MBP (1:300 ...
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bioRxiv - Neuroscience 2020Quote: ... the neurons were fixed and stained as previously described38 using primary antibodies for β-3-Tubulin (TUJ1; 1:200; BioLegend, San Diego, CA, USA; 801201) and TH (1:300 ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was discarded and the cell pellets were resuspended in PBS containing Zombie NIR™ dye (Biolegend; 1:200 in PBS; 100μl per well) and incubated at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was discarded and the cell pellets were resuspended in PBS containing Zombie NIR™ dye (Biolegend; 1:200 in PBS; 100μl per well) and incubated at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 μm sections were sliced and stained with the murine version of the mAbs (1A8 muIgG2a-4m and 7C8 muIgG2a-4m) and compared to the anti-Aβ N-terminus positive control antibody (6E10, Biolegend, Cat. # #SIG39320, diluted 1:1000) and an IgG negative control antibody (used at a concentration of 5 μg/ml) ...
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bioRxiv - Cancer Biology 2022Quote: Epithelial crypt cells from the ileum of Ki67-RFP ApccKO/cKO Villin-CreERT2 mice and microadenomas from Rosa26-tdTomato ApccKO/cKO Lgr5-EGFP-CreERT2 mice were stained with Pacific Blue™ (PB) anti-CD45 antibody (#103126, BioLegend, USA; dilution 1:200), PB-conjugated anti-CD31 (#102422 ...
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bioRxiv - Immunology 2024Quote: ... Yeast cells were washed two times with PBSF and subsequently stained for 30 minutes on ice with a cocktail of anti-HA APC antibody (Biolegend, Clone: 16B12, dilution 1:100), goat F(ab′)2 anti-human IgG PE (SouthernBiotech ...
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bioRxiv - Developmental Biology 2024Quote: ... The cells were pelleted and resuspended in FACS buffer for staining with the following antibodies for 20 min at 4° C: Ter119-FITC (BioLegend, Catalog No. 116205, 1/100), EpCAM-PE (BioLegend ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were then washed with FACS buffer and incubated with the following Lineage antibodies for 30 min at 4°C: CD11b Biotin (BioLegend, Catalog No. 101204, 1/1000), CD11c Biotin (BioLegend ...
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bioRxiv - Systems Biology 2023Quote: ... All iPSCs were stained in PBS with 5% FBS overnight at 4°C with the following antibodies and dilutions: Alexa Fluor® 647 anti-tubulin α (1:1000, Biolegend, #627908, clone 10D8), and Alexa Fluor ® 488 anti-Oct-4A (1:500 ...
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bioRxiv - Systems Biology 2023Quote: ... were used at a concentration of 1:100 then stained with anti-rabbit-PE or anti-mouse Pacific Blue secondary antibody (BioLegend® #406421, Life Technologies #P10993) for flow cytometry.
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bioRxiv - Immunology 2023Quote: ... and incubated with anti-BTN3A antibody (clone 20.1) or IgG1,κ isotype control (clone MOPC-21, BioLegend; or BM4-1, a gift from CSL Limited) at 5 µg/mL for 15 min at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were resuspended in 10 μl of FACS Buffer and incubated with 1 μl of allophycocyanin (APC)-conjugated anti-CD46 antibody (BioLegend, clone TRA-2–10, 352405) for 30 min at 4°C with rotation ...
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bioRxiv - Molecular Biology 2024Quote: ... the supernatant was transferred to a tube containing 10 µL of fresh beads and 1 µL of α-HA antibody (901515, BioLegend, San Diego, California, U.S.) and incubated under gentle agitation overnight at 4° C ...
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bioRxiv - Pathology 2022Quote: ... and cells were stained in FACS buffer for 30 min at 4°C with the following anti-mouse antibodies: PacificBlue-conjugated anti-CD45 (Biolegend, clone 30-F11, #103126, 1/100), PE-Cy7-conjugated anti-CD11b (BD Biosciences ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Immunofluorescence staining was performed in 96 well plates on seminiferous tubules with primary antibodies major histocompatibility complex class II [MHC Class II RT1D (BioLegend, Clone OX-17, 205401, 1:200)] ...
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bioRxiv - Immunology 2020Quote: ... re-suspended in flow cytometry staining buffer (FACS) buffer containing 1:100 dilution of target-specific antibodies (anti PD1:cat≠329905; Biolegend, and anti-PDL1: Cat≠329705; Biolegend) and incubated for 30 minutes at 4°C in the dark ...
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bioRxiv - Immunology 2024Quote: ... cells were centrifuged at 900 x g for 5 minutes at room temperature and stained with Zombie Aqua Fixable viability kit (Biolegend, 1:1000 diluted in PBS, 100µL) for 20 minutes at room temperature in the dark to enable exclusion of dead cells ...