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Citations for BioLegend :
8401 - 8450 of 8901 citations for DNAM 1 Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysates were cleared by centrifugation and β1 integrin was immunoprecipitated with anti-β1 integrin (clone HMb1-1, BioLegend, #102202) and Dynabeads Protein G (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight at 4°C in primary antibodies diluted in 1-5% serum in PBS-T according to the following concentrations: novel Parkin mAbs from Biolegend clones D (BioLegend ...
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bioRxiv - Cell Biology 2021Quote: ... Tissue sections were then washed and stained with an AF647-directly conjugated antibody towards CCR6 (1:200, clone G034E3, BioLegend) at 37 ° C for one hour ...
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bioRxiv - Genetics 2020Quote: ... and stained at 4°C in a final volume of 50µL with the following antibodies: CD14-FITC (1:50)(Biolegend 301803), CD16-PE (1:50)(Biolegend 3G8) ...
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bioRxiv - Microbiology 2021Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend)) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2020Quote: A total of 1 x 106 mononuclear cells suspended in PBS were stained using fixable viability dye (Zombie NIR-Biolegend), and incubated for 15 minutes at room temperature following manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2021Quote: ... Mononuclear cells were incubated with APC (allophycocyanin) or PE (phycoerythrin)-conjugated anti-mouse Ly-6A/E (Sca-1) antibody (#122508, BioLegend), APC or PE-conjugated anti-mouse CD45 antibody (#103106 ...
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bioRxiv - Cell Biology 2020Quote: ... Measurements were compared to the isotopic control (APC-conjugated anti-mouse IgG1, Biolegend clone MOPC-21 #BLE400122, 1/100, Ozyme) to determine background and positivity thresholds ...
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bioRxiv - Cancer Biology 2021Quote: ... For the assessment of CD71 expression in TF-1 and DAMI cells were labeled with the phycoerythrin (PE)-cyanine 7 (Cy7)-conjugated CD71 (BioLegend) (#113811) ...
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bioRxiv - Cell Biology 2020Quote: Jurkat cells expressing both the GalT-GFP-SNAP and the HA-LAT encoding constructs were incubated for 30 min at 4°C on a wheel with 1:100 of mouse anti-HA Ab (901515; Biolegend). The cells were washed with cold PBS and incubated for 45 min at 4°C on a wheel with membrane-impermeable BG–PEG9–NHS29 ...
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bioRxiv - Immunology 2022Quote: ... the processed fecal samples are incubated with autologous diluted serum and stained as previously described with the following fluorophore-conjugated antibodies: goat anti-mouse PE-IgA and PE-Cy7-IgG1 (clone RMG1-1; Biolegend). Flow cytometry analysis on stained bacteria populations was performed on the LSRFortessa and data analysis was performed on FlowJo ver ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were next incubated in 1x BD Perm/Wash buffer containing AF674-conjugated anti-Ki67 antibody (16A8; Biolegend; 1:100) at 4°C overnight ...
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bioRxiv - Bioengineering 2022Quote: ... lot: B266946), Ly-6A/E/Sca-1 (D7, lot: B249343), and CD117/cKit (2B8, lot: B272462) were purchased from Biolegend. Anti-mouse F4/80 (BM8 ...
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bioRxiv - Bioengineering 2022Quote: ... Splenic cells at 1 × 106 were co-cultured with target cells at 1:2 effector to target ratio in the presence of anti-CD107a (Biolegend), brefeldin A and monensin and a serial dilutions (6x ...
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bioRxiv - Biophysics 2022Quote: ... were established by transfection of pIRES-HA-CXCR4 and pIRES-HA-CXCR4-W1955.34L and subsequent cell sorting for equivalent surface expression levels using 1:1000 Alexa Fluor 488-labeled anti-HA antibody (clone 16B12, Biolegend). U87 cells were grown in Dulbecco’s modified Eagle medium (Themo Fischer Scientific ...
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bioRxiv - Biophysics 2022Quote: ... Equivalent surface expression level was verified using an Alexa Fluor 488-conjugated anti-HA-tag mAb (clone 16B12, 1:1000 Biolegend). Flow cytometry data were analyzed using FlowJo V10 software.
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bioRxiv - Immunology 2022Quote: ... The following antibodies were used to identify MKs: 1:100 anti-mouse CD41-FITC+ and anti-mouse CD42d-APC+ (BioLegend); and MKPs ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with primary antibodies (anti-GFAP, DAKO, z-0334, 1:1000; SMI-99 [anti-myelin basic protein] Biolegend; 808401 ...
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bioRxiv - Immunology 2022Quote: ... The clone and dilution of antibodies used for flow cytometry were as follows: CD4-Pacific Blue (Biolegend, OKT4, 1:200), CD69-APC (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... the cells were stained with a live/dead fixable dye (Zombie Red dye, 1/800 dilution – BioLegend, San Diego, CA) for 20 minutes at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked in TBS-T/5% BSA for one hour at RT and incubated overnight at 4 °C with anti-P62 (SQSTM1) (1:500, #814802, BioLegend), anti-CD107b (LAMP-2 ...
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bioRxiv - Molecular Biology 2022Quote: ... Alexa Fluor® 488-conjugated or PE/Cy7-conjugated rat anti-mouse CD326 (EpCAM) antibody (1:200, BioLegend, clone G8.8), Alexa Fluor® 647-conjugated rat anti-mouse CD104 (integrin β4 ...
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bioRxiv - Physiology 2022Quote: ... Samples were incubated with anti-c-kit rat monoclonal antibody (1:500 v/v, ACK2, BioLegend, San Diego, CA, USA) overnight (4ºC) ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg biotinylated SARS-CoV-2 BA.1 protein (His & AVI Tag) (SinoBiological, 40592-V49H7-B) conjugated with PE-streptavidin or APC-streptavidin (TotalSeq-C0971 Streptavidin, BioLegend, 405271 and TotalSeq-C0972 Streptavidin ...
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bioRxiv - Immunology 2022Quote: ... Samples were centrifuged at 400g for 3 minutes at room temperature and resuspended 50μL in PE-Annexin V antibody diluted 1:20 with Annexin V Binding Buffer (BioLegend, 422201), or 50uL Annexin V Binding Buffer only for controls ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed once with cold PBS and then stained with 100 µL Zombie Aqua (BioLegend; 1:1000 in PBS) for 30 min at RT ...
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bioRxiv - Immunology 2022Quote: ... samples were stained with the following antibodies in 1X eBiosciences permeabilization buffer for 30 minutes at room temperature: T-bet (1:100; clone 4B10; BioLegend); Foxp3 (1:300 ...
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bioRxiv - Cell Biology 2022Quote: One million fixed BM and spleen cells were immunolabelled for one hour at room temperature with Ter119-PE (1:200, Biolegend) and 1ug/ml Hoechst 33342 (Thermofisher ...
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bioRxiv - Neuroscience 2021Quote: ... followed by Biotin-SP-conjugated AffiniPure Goat Anti-Rabbit IgG at a dilution of 1:200 for 30 min at room temperature and incubation with horseradish peroxidase streptavidin at a dilution of 1:125 (405210, BioLegend) for 30 min at room temperature ...
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bioRxiv - Immunology 2020Quote: ... and 1.0-2.5 x 104 NK cells were cultured in K10 media along with either IL-15 alone (1-100 ng/mL; BioLegend), or a cocktail of IL-12 (10 ng/mL ...
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bioRxiv - Immunology 2020Quote: The cytokines IFNγ and TNFα contained in supernatants derived from 1-4 h BxPC3 - CAR T cell co-cultures were quantified by ELISA using Abs from Biolegend.
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bioRxiv - Neuroscience 2020Quote: ... pH 7.4)-fixed coronal brain sections were blocked with 10% donkey serum (Jackson Immunoresearch)/PBS plus 0.3% Triton X-100 in PBS and incubated with antibodies to p-S129-α-synuclein (1:1000; Biolegend), and TH (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were incubated with 1.5% normal serum followed by incubation with the primary antibody (biotinylated anti-Aβ – 1:100, BioLegend, USA) for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: After 7 days of shRNA-mediated knockdown 1 x 105 cells were wash once in PBS and resuspend in Annexin-V Binding Buffer (422201, BioLegend) with 3 μl of Annexin-V-APC (640941 ...
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bioRxiv - Immunology 2020Quote: ... Antibody dilutions in cell staining buffer containing 1 % TruStain fcX™ (anti-mouse CD16/32) Antibody (both BioLegend, London, UK) were used to stain according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were washed again 3 × 5 minutes in PBS and incubated overnight with rat anti-phospho-TDP primary antibody (1:100, 829901, BioLegend), in a dilution buffer (0.05% Tween ...
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bioRxiv - Cell Biology 2021Quote: ... Populations of interest based on DAPI negativity expression and lack of lineage markers (APC-Cy7; CD31, Ter119, CD45 at 1 μL/106 cells; Biolegend) expression were directly sorted into DMEM/F12 containing 10% FBS with 1% P/S/A ...
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bioRxiv - Cell Biology 2020Quote: ... washed with 0.5 % BSA/PBS and incubated with 200 µL of a 1:50 dilution of hCD4-BV711 (OKT4, BioLegend, UK) for 30 min ...
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bioRxiv - Immunology 2020Quote: ... Effector function was assessed by stimulating cells for 4 hours with PMA (20 ng/ml) and Ionomycin (1 μg/ml) with GolgiStopTM and monitored for CD8-PerCP (RPA-T8, Biolegend), CD4-AF700 (RPA-T4 ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained at 4°C for 30 min in Hanks Balanced Salt Solution-2% FBS with the following markers: anti-THY1 AF700 1:100 (Biolegend), anti-PDGFRalpha PE 1:100 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... Cells from each individual mouse were stained using BioLegend TotalSeq B hashtags (Hashtags 1-4) and an anti-PE oligonucleotide barcode (BioLegend). Cells from each compartment (i.e ...
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bioRxiv - Developmental Biology 2022Quote: ... MGs were localized using the mT/mG reporter or stained using a rabbit anti-K14 polyclonal antibody (1:500, 905301, BioLegend). Nuclei were counterstained with DAPI ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... to block nonspecific binding and then cells were stained using a master mix of CD45.1 (Biolegend Cat # 110728, 1:1000), F4/80 (Biolegend Cat # 123110 ...
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bioRxiv - Immunology 2022Quote: ... On day 6 BMDM were replated at 1 × 106 cells per well in 24-well plates and stimulated with 10 µg/ml Imiquimod (Biolegend) with 10 µg/ml anti-CD200R1 (OX131 ...
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bioRxiv - Immunology 2022Quote: ... The BM cells per animal were spined down and then resuspended in 1 mL Red Blood Cell Lysis (RBCL) buffer (Biolegend) at room temperature for 1 min to lyse the red blood cells ...
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bioRxiv - Immunology 2022Quote: ... LN and thymic homogenates were stained with a cocktail of antibodies against CD4-FITC and CD25-APC (for LN Tregulatory cells; Mouse Regulatory T Cell Staining Kit #1, eBioscience) or CD4-PB (Biolegend), CD8-FITC ...
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bioRxiv - Immunology 2022Quote: ... at a 1:200 dilution for 30 mins at room temperature and was detected with anti-rabbit BV421 (Dnk, BioLegend). Flow was performed on Cytek Aurora (Cytek ...
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Orchestration of alternative splicing regulates bone marrow mesenchymal stem cells fate during agingbioRxiv - Cell Biology 2022Quote: ... We flushed bone marrow cells from the tibia and femora of male mice and incubated the cells with anti-Sca-1-PE (108108; BioLegend), anti-CD29-FITC (102206 ...
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bioRxiv - Systems Biology 2022Quote: ... The population corresponding to cluster B was sorted by top 1% of CD49c abundance via red florescent antibody (Biolegend 343808). Cells corresponding to cluster A were sorted from 25-75% abundance range ...
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bioRxiv - Cancer Biology 2022Quote: Cells were collected in suspension post digest and at each passage and were stained with a live/dead marker Zombie UV (1:1000, Biolegend) for 30 min at room temperature in DPBS (Gibco) ...