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Citations for BioLegend :
8451 - 8500 of 8901 citations for DNAM 1 Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... The cells were pelleted by centrifugation and washed in FACS buffer before incubation with goat anti-mouse Alexa Fluor 647 (diluted 1:200, Biolegend) for 30 minutes on ice ...
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bioRxiv - Immunology 2019Quote: ... glass slides or 8 well chambers were coated with either ICAM-1 alone or anti-CD3 (clone Hit3a from Biolegend)/anti-CD28 (clone CD28.2 from Biolegend)/ICAM-1 (R&D systems ...
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bioRxiv - Immunology 2019Quote: ... Some cultures were supplemented with combinations of 8µg/ml IL-34 (Generon, UK), 100 µg/ml BMP7 (RD systems, USA and 10µg/ml CSF-1 (Biolegend, USA) for the duration of the culture.
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bioRxiv - Cancer Biology 2019Quote: ... approximately 1 * 106 cells from dLN and 5 × 106 tumor cells were first stained with Zombie Red Fixable Viability Kit (BioLegend) for 30’ at room temperature and then incubated on ice for 15’ with an anti-mouse CD16/32 antibody (BioLegend) ...
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bioRxiv - Cancer Biology 2020Quote: ... Tumor sections were then incubated with fluorochrome-conjugated antibody (1:100, Alexa Fluor 488 anti-mouse F4/80, #123120, BioLegend; or PE anti-mouse CD40 ...
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bioRxiv - Cancer Biology 2020Quote: ... Tumor sections were then incubated with fluorochrome-conjugated antibody (1:100, Alexa Fluor 488 anti-mouse F4/80, #123120, BioLegend; or PE anti-mouse CD40, #124610, BioLegend; or PE anti-iNOS ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were either stained with DAPI for viability or fixed with 1% PFA and stained with zombie aqua fixable viability kit (Biolegend). Viable cells were sorted on a FACSAria flow cytometer (Becton Dickinson) ...
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bioRxiv - Immunology 2019Quote: ... and incubated for 1 h at 4°C in the dark: CD3 (anti-CD3-APC-780; clone 145-2C11, Biolegend), CD4 (anti-CD4-PE-Cy7 ...
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bioRxiv - Bioengineering 2020Quote: ... Unbound cells were collected and cultured for 24 hours in the presence of 1 μg/ml anti-mouse CD3ε (145-2C11, BioLegend) and 0.5 μg/ml CD28 (37.51 ...
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bioRxiv - Immunology 2020Quote: ... the biotinylated proteins were incubated for a minimum of 1 h at 4°C with the streptavidin-conjugates AF647 (Biolegend), BV421 (Biolegend ...
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bioRxiv - Neuroscience 2019Quote: ... followed by overnight incubation at 4°C with either anti-beta amyloid 6E10 (mouse, 1:1,000 dilution of 1mg/mL stock) (Biolegend, USA), anti-tau MC1 (mouse ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight at 4°C in primary antibodies diluted in 1-5% serum in PBS-T according to the following concentrations: novel anti-parkin mAbs from Biolegend clones D (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... REA159), Brilliant Violet 421 (BV421)-conjugated anti-pSTAT3 (pY705, 13A3-1) or BV421-conjugated anti-pAKT (pS473, M89-61) (Biolegend) Abs.
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bioRxiv - Microbiology 2019Quote: ... The cells were then washed with ice-cold FACS Buffer (1x PBS + 3% FBS) before staining with mouse anti-HA (diluted 1:200, Biolegend) for 30 minutes on ice ...
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bioRxiv - Developmental Biology 2019Quote: ... digested enzymatically with a papain-based solution (Sumitomo Bakelite) and then resuspended in 0.3% BSA/PBS containing primary antibodies (PE-conjugated CD133 (mouse, 1:500, BioLegend, 141204) and APC-conjugated CD24 (mouse ...
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bioRxiv - Immunology 2019Quote: ... Approximately 106 cells/sample were re-suspended in PBS and dead cells removed using amine-reactive dye NIR Zombie (1:500 dilution in the antibody master mix; #423105, BioLegend). Live cells were stained with the following cell surface marker specific antibodies ...
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bioRxiv - Immunology 2019Quote: ... Tissue sections were then rinsed in staining buffer (1% BSA, 0.03% Triton X-100 in PBS) and incubated with anti-β-tubulin III Alexa 594 (TUBB3, Biolegend), anti-VIP Alexa 647 (H-6 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were blocked in 10% BSA in PBS for 1 h at room temperature and then incubated with a MAP2 antibody diluted in 3% BSA (MAP2 chicken, 1:10000, Biolegend) for 60 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... and subsequently stained for 1 hr at room temperature with anti-H2AX-Phosphorylated (Ser139) antibody conjugated with Alexa Fluor 647 (Biolegend), or Anti-p-CHK1 antibody conjugated with PE (Cell Signaling ...
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bioRxiv - Genomics 2021Quote: Cells from the completed cTSA-seq reaction were resuspended in PBS containing 0.001% Tween-20 and Streptavidin conjugated to Alexa 488 (1:200, Biolegend, #405235) and an anti-rabbit secondary conjugated (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... was detected using biotin labelled anti-mouse IgG1 and IgG2a while total IgG (dilution 1:1000) was detected using anti-mouse HRP conjugated IgG followed by anti-mouse streptavidin-HRP (Biolegend). The plates were further incubated at 37°C for 1h and washed 7 times with a washing buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were resuspended for 30 min in 100 μL of flow cytometry buffer and anti-ICAM-1 (Pe-Cy7, Biolegend) and anti-VCAM (APC ...
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bioRxiv - Cancer Biology 2021Quote: ... in 100ul PBS for 10min at RT followed by surface staining with antibody specific for CD45 (30-F11, PercPcy55, 1/300, Biolegend). Tumor cells (for RNA-seq ...
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bioRxiv - Cancer Biology 2020Quote: ... and labelled via incubation with 1:100 dilution of Alexa Fluor 488 conjugated anti-CD45 antibody (Cat. no. 304017, BioLegend) and 1:100 dilution of PECy7-conjugated anti-CD31 antibody (Cat ...
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bioRxiv - Cell Biology 2020Quote: Single cell suspension was generated from adult lungs and stained with antibodies: anti-EpCAM (APC-Cy7-conjugated, Biolegend,1:50), CD49F (APC-conjugated ...
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bioRxiv - Cell Biology 2020Quote: ... The virus particles were stained at a concentration of 5×108 particles mL−1 and labeled with anti-GFP PE antibody (Clone FM264G, BioLegend) at 0.4 μg mL−1 in a 100 μL staining volume for a minimum of 30 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: Bone marrow cells from two 8-week-old wild-type mice were each stained with 1 μg of different hashtag antibodies (TotalSeq A0301 and TotalSeqA0302, respectively; BioLegend). At the same time ...
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bioRxiv - Immunology 2021Quote: ... Cells were then incubated with bead/Ab mixture for 1 hr at 37°C and then stained with PacificBlue-conjugated anti-CD66b (Biolegend) in PBS ...
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bioRxiv - Immunology 2021Quote: ... The antigenicity and the immunogenicity of the purified recombinant spike protein was validated (Supplementary Fig 1) against verified available recombinant spike protein (BEI Resources, NR52397) and anti-SARS-CoV-2 spike antibody clone A20085C (BioLegend).
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bioRxiv - Immunology 2021Quote: ... Cells were washed and stained with 100 μl of 1:1000 dilution of fixable Zombie Aqua (BioLegend, San Diego, CA) for 20 minutes at 4°C in dark ...
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bioRxiv - Immunology 2020Quote: ... buffer containing 1:100 dilution of target-specific antibodies (anti PD1:cat≠329905; Biolegend, and anti-PDL1: Cat≠329705; Biolegend) and incubated for 30 minutes at 4°C in the dark ...
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bioRxiv - Immunology 2020Quote: ... The pellet was resuspended in flow cytometry staining buffer (FACS) buffer containing 1:100 dilution of CD3 antibody (Cat≠317307; Biolegend) and incubated for 30 minutes at 4°C in the dark ...
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bioRxiv - Immunology 2021Quote: ... or total combined splenocytes plus bone marrow from WT or c-Rel-/- mice were stimulated for 48 hours in complete RPMI containing 10% fetal bovine serum (FBS) and 1% pen strep with either rmIL-2 (1000 U/ mL; BioLegend), rmIL-15 (100 ng/mL ...
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bioRxiv - Immunology 2021Quote: ... and finally with a mixture of AlexaFluor 647-conjugated rat anti-mouse Ly6C antibody (BioLegend, clone HK1.4; diluted 1:500) and DAPI (Molecular Probes ...
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bioRxiv - Microbiology 2021Quote: ... for 15 min at 4°C prior to wash in 1 ml PBS and centrifugation at 1600 rpm for 5 min and surface molecule staining with CD206 APCCy7 (BioLegend), and intracellular staining of anti-human Arginase1 FITC (Ref ...
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bioRxiv - Immunology 2020Quote: ... followed by incubation for 1h at RT in a 1:1000 dilution in blocking buffer of primary Ab (purified anti-Filaggrin polyclonal Ab, clone Poly19058, Biolegend). After washing ...
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bioRxiv - Immunology 2020Quote: ... lymph nodes were removed from Myc-eGFP mice and mashed and cell suspension activated with anti-CD3 (1 µg/mL, clone 145-2C11, Biolegend) and anti-CD28 (3 µg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transferred to a round-bottomed 96-well plate and were incubated with an AF647-conjugated anti-GFP antibody (1:20, BioLegend) and fixable viability dye eFluor 780 (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... washed twice with True Nuclear Permeabilization/Wash buffer and then stained for 1 hour at room temperature with FoxP3 BV421 (206D, BioLegend), Ki-67 BV711 (Ki-67 ...
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bioRxiv - Genomics 2021Quote: ... Then cells were stained with the following cell surface marker antibodies for 30 min on ice (1:100 dilution, all from BioLegend): CD11b-PE (301306 ...
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bioRxiv - Immunology 2022Quote: ... propidium iodide (eBioscience) was added to a final dilution of 1:250 and Annexin V Alexa Fluor™ 647 (Biolegend) was used at 1:125 dilution ...
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bioRxiv - Immunology 2022Quote: ... FSC-A/H and dead cell exclusion by using a Fixable Viability Kit (Near-IR staining, dilution 1:500, Biolegend).
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bioRxiv - Immunology 2022Quote: ... PBMC collected 17 weeks post-boost were incubated for 10 min on ice with purified CD16 (3G8, BioLegend, 1:100), CD32 (FUN-2 ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in FACS buffer (1% BSA in PBS) at a concentration of 108 cells/mL and 1 μL negative CD8+ T cell biotin-antibody cocktail (Biolegend)/million cells was added and allowed to incubate for 15 min on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... is the complete list of antibodies used for experiments of T cell activation or exhaustion against primary AML: Anti-CD279 (PD-1) FITC (cat#329904 from Biolegend), CD366 (TIM-3 ...
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bioRxiv - Cancer Biology 2022Quote: ... After cell counting 1×106 cells were stained with a cocktail for lineage markers (CD3, CD11b, B220, Ter119, and Ly6G/6C; BioLegend), and lineage-negative cells (Lin- ...
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bioRxiv - Genomics 2022Quote: ... Thermo Cat# 00-5523-00) followed by intra-cellular stains with anti-mouse IFNψ-PerCP/Cyanine5.5 (1:200, clone XMG1.2, BioLegend Cat#505821), T-bet monoclonal antibody-PE-Cyanine 7 (1:200 ...
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bioRxiv - Immunology 2022Quote: ... They were then stained overnight at 4ºC with the following antibodies: α-smooth muscle actin (αSMA for myofibroblast, AF488 1:100, Biolegend). Following primary staining ...
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bioRxiv - Immunology 2022Quote: ... Cells from each sample were stained individually for 30 mins with a master mix containing the antibodies specific to CD45-FITC (1:100, Biolegend) and a TotalSeq Hashtags Antibody marker (Biolegend) ...
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bioRxiv - Immunology 2022Quote: Cells isolated from murine livers were stained with 30 μg/mL Hoechst 33342 (Miltenyi) and Zombie NIR viability dye (diluted 1:250 in PBS; Biolegend) before FACS sorting (Sony SH800 ...