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Citations for BioLegend :
8651 - 8700 of 8901 citations for DNAM 1 Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Plates were washed three times with PBS-T (PBS with 0.1% Tween-20) and 50μl of mouse IgG-specific secondary antibody (BioLegend #405306, 1:10,000) diluted in dilution solution added to each well ...
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bioRxiv - Immunology 2020Quote: ... were added to the tumor cells and incubated for 2 hours with CD20-TCB (200 ng/ml) and +/- αLFA1 antibody (Clone: TS 1/18, 10 μg/ml, BioLegend, 302116). Cells were then gently washed ...
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bioRxiv - Developmental Biology 2021Quote: ... The following antibodies were added at the concentration of 1:50 and incubated on ice for 45 minutes: APC/Cy7 Cd45 (Biolegend #103116), APC Pecam1 (Biolegend #102410) ...
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bioRxiv - Immunology 2022Quote: Cryopreserved PBMCs were thawed and washed twice with 10 ml of FACS buffer (1 x PBS containing 2% FBS and 1 mM EDTA) and resuspended in 100 μl of 1x PBS containing Zombie UV live/dead dye at 1:200 dilution (BioLegend, 423108) and incubate at room temperature for 15 minutes ...
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bioRxiv - Immunology 2022Quote: ... with the following modifications: Approximately 1 million cells per sample were resuspended in 95 ul of cell staining buffer (Biolegend, USA) with 5 ul of Fc receptor Block (TrueStain FcX ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were then treated with 25 μl of APC-antiHA antibody with 1:75 dilution (~0.07 μg/well, BioLegend catalog # 901523) for 1 hour on ice in the dark ...
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bioRxiv - Immunology 2019Quote: ... Antibody coated cells were mixed with resting NK cells in the presence of 1 μl BV421 anti-CD107a mAb for 2 hr at 37°C (H4A3, Biolegend 328626). Cells were then stained with mAbs to KIR2DS4 (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2019Quote: ... Purity of isolated cells was validated by FACS by using the cell surface markers EpCAM-APC (Biolegend, 118213, 1:50 dilution) and CD45-PE (Biolegend ...
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bioRxiv - Genomics 2019Quote: ... The following antibodies were added (1:100) to each sample and incubated for 30 minutes at room temperature: Alexa Fluor 700 anti-mouse CD8a (Biolegend, 100730), PE/Dazzle-594 anti-mouse CD4 (Biolegend ...
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bioRxiv - Cancer Biology 2019Quote: ... and stained with the following antibodies in isolation or in combination for 30-60 minutes in the dark at 4°C: ICAM-1 PE (BioLegend, #353106), CCR6 PE/Dazzle (BioLegend ...
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bioRxiv - Neuroscience 2021Quote: ... All slides were processed at the same time using the purified mouse monoclonal anti-beta-amyloid 1-16 antibody (6E10) (Biolegend, 803001) at 1/500 for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained with the following primary mouse antibodies (1:100 dilution) for 30 minutes at 4°C: CD45-BV750 (clone 30-F11, Biolegend, 103157), CD31-PE-Cy5.5 (clone MEC 13.3 ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed again and then incubated with horse radish peroxidase (HRP) conjugated anti-mouse IgG secondary antibody (Biolegend; 1:3000) for 1 hour at 37°C ...
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bioRxiv - Immunology 2021Quote: ... Approximately 2 million cells per experimental condition were stained with 1 μg of TotalSeq™ B0301-B0308 anti-mouse Hashtag antibodies (BioLegend) according to the manufacturer’s instructions and pooled together at equal cell numbers ...
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Immunoresolvents Support Skeletal Myofiber Regeneration via Actions on Myeloid and Muscle Stem CellsbioRxiv - Immunology 2020Quote: ... Cells were resupsended and incubated for 30 min on ice with primary antibodies including Sca-1-APC (Biolegend [Clone D7; 108112]), CD45-AF488 (Biolegend [Clone 30-F11 ...
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bioRxiv - Immunology 2021Quote: ... PD-L1 expression in primary tumor-associated LECs was determined using CD45-PE/Cy7 (clone 30-F11, Biolegend 103114, 1:200), CD31-PerCp/Cy5.5 (clone MEC13.3 ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed three times with PBS-T (PBS with 0.1% Tween-20) and 50μl of mouse IgG-specific secondary antibody (BioLegend #405306, 1:10,000) diluted in dilution solution added to each well ...
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bioRxiv - Microbiology 2022Quote: ... bacteria were labeled with 5 µg ml−1 carboxylfluorescein diacetate succinimidyl ester (CFSE) or Tag-IT Violet™ proliferation and cell tracking dye (Biolegend) in PBS containing 5 mM MgSO4 ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were incubated overnight at 4 °C with primary antibody (Biotin anti-β-Amyloid, 1-16 Antibody, clone 6E10 from BioLegend) dissolved in a carrier solution made with 1% HS ...
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bioRxiv - Cancer Biology 2022Quote: ... The washed samples were stained for 20 min on ice with Brilliant Violet 510™ anti-mouse CD45 Antibody (1:200, Clone: 30-F11, BioLegend), PE/Cyanine7 anti-mouse/human CD11b Antibody (1:200 ...
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bioRxiv - Bioengineering 2022Quote: ... CMs in the ORR field of view were uniquely identified by staining with CM surface maker SIRPa (1:100, Biolegend, 323802). We performed control studies to ensure that the SIRPa signal did not interfere with ORR measurement (Supplementary Fig ...
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bioRxiv - Immunology 2022Quote: ... single-cell spleen suspensions from C57BL/6J mice were cultured at 5 million cells/ mL in complete RPMI with 1 μg/mL anti-CD3 (BioLegend, #100340) and 1 μg/mL anti-CD28 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... and incubated with the following antibodies in cRPMI at 1:200 for 30 minutes: CD4-BV650 (#100469; Biolegend, San Diego, USA), CD8-BV605 (#100744 ...
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bioRxiv - Immunology 2022Quote: ... The cells were resuspended in 1 ml FACS buffer and stained with Zombie Near-IR Live/Dead (Biolegend, Cat. No. 423105), CD11b-Pacific Blue (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... Immunostaining was performed by incubating the sections overnight at 4°C with the SMI-32 primary antibody (Biolegend: Suppl. Table 1) in incubation buffer ...
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bioRxiv - Synthetic Biology 2022Quote: ... The blocking buffer was subsequently removed and cells were incubated for an additional hour with 1:100 diluted primary antibody (αHA antibody, Biolegend, #901502) in antibody dilution buffer (1x PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... the NHE1 CRISPR silenced organoids and Lgr5DTR-GFP organoids were first dissociated and then stained for CD44 (BioLegend, 103030, 1:250), CD24 (BD ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated in primary antibodies diluted in 5% skim milk in TBST: mouse anti-brevican (1:1000, BioLegend, cat# 820101), sheep anti-neurocan (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Then the live microglial cells were incubated with PBS supplemented with 5% BSA + HLA-DR mouse monoclonal antibodies pre-conjugated with FITC (1:100, BioLegend, Inc.) + Hoechst 33342 (1:5000 ...
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bioRxiv - Synthetic Biology 2022Quote: ... The membrane was incubated with 5% nonfat milk in TBST for 1 hour followed by the addition of primary antibodies (BioLegend, 652505). After 1 hour of the primary antibody incubation ...
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bioRxiv - Cancer Biology 2022Quote: ... for 10 min at 4°C followed by an incubation with CD45-BV421 (Biolegend 103133, clone 30-F11, 1:200 dilution) and Ter119-BV421 (Biolegend 116233 ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissue sections were stained with monoclonal mouse anti-βIII-tubulin primary antibody (Biolegend, Cat. 801202; 1:100 overnight at 4 °C) and goat anti-mouse AF647 (abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... then incubated with their respective primary antibodies diluted in 5% skim milk in PBST at 4 °C overnight: purified mouse anti-βIII-tubulin (1:1,000) (BioLegend, cat. 801202), monoclonal mouse anti-GAPDH (1:50,000 ...
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Autocrine Sfrp1 inhibits lung fibroblast invasion during transition to injury induced myofibroblastsbioRxiv - Cell Biology 2022Quote: ... an aliquot of 3×105 cells in 1ml MACS buffer was incubated at RT for 15 minutes with 1:500 dilution of Zombie UV™ Fixable Viability Kit (Biolegend). Cells were stained with 1 µl of anti-GFP antibody (Santa Cruz ...
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bioRxiv - Cell Biology 2023Quote: ... breast cancer cells and myeloma cells were treated with recombinant hTRAIL (B294007, Biolegend, 1 to 300 ng/ml for different cells) for 3 h in the presence or absence of either HL-III (5 milliunits/ml) ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... The production of cytokines and cytolytic mediators was determined in OT-1 T-cells using intracellular cytokine staining as previously described93,94 to determine interferon-gamma (anti-IFN-γ PE, Biolegend, # 505807), tumor necrosis factor-alpha (anti-TNF-α FITC ...
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bioRxiv - Bioengineering 2024Quote: ... were coated with 100 µL of 4 µg/mL polyclonal anti-lysostaphin antibody (AICBIOTECH-PAb102) in 1×ELISA coating buffer (BIOLEGEND-421701). Wells were washed with 0.05% Tween 20 (SIGMA-P9416-50ML ...
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bioRxiv - Neuroscience 2023Quote: ... After incubation, cells were centrifuged (350rcf, 7min, 4°C) and resuspended in permeabilization buffer containing Streptavidin (PerCP, Biolegend #405213, 1:600) and antibodies directed against intracellular targets Foxp3 (APC ...
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bioRxiv - Genetics 2024Quote: ... was collected in EDTA-coated capillary and was stained for 15 min in the dark at room temperature with APC labelled anti-mouse CD45.1 antibody (Biolegend, 1:20) and PE labelled anti-mouse CD45.2 antibody (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed with FACS buffer (PBS+ 2% (v/v) FBS with 1mM EDTA) and incubated with a 1:500 dilution of TruStain FCX (Biolegend, 101320) for 5 minutes ...
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bioRxiv - Immunology 2024Quote: ... Cells were next stained for 30 mins on ice with a 1:300 dilution of antibodies raised against mouse CD206 (Biolegend,141710), CD301(Biorad ...
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bioRxiv - Immunology 2023Quote: ... and incubated in 100 μl of Maxpar Cell Staining Buffer with TruStain FcX™ (anti-mouse CD16/32) Antibody (1:100; 101320, Biolegend) for FC blocking (10 min ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then stained with standard antibodies for immune and vascular lineage exclusion: anti-CD45-PE/Cy7 (BioLegend, 304016, 1:200), anti-CD31-PE/Cy7 (BioLegend ...
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bioRxiv - Synthetic Biology 2022Quote: ... The membrane was incubated with 5% nonfat milk in TBST for 1 hour followed by the addition of primary antibodies (BioLegend, 652505). After 1 hour of the primary antibody incubation ...
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bioRxiv - Microbiology 2023Quote: ... were stained with Zombie Aqua™ dye at 1:100 in PBS and washed with PBS following the manufacturer’s protocol (BioLegend, 423101). Samples were then stained for 10 minutes at 4°C with a rabbit anti-AnTat1.1 polyclonal antibody diluted 1:15,000 in HMI-9 media and washed once with HMI-9 (antibody courtesy of Jay Bangs) ...
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bioRxiv - Immunology 2023Quote: ... Flow cytometry was performed to assess neutrophil purity by staining the neutrophils at a 1 in 200 dilution for Ly6G (BioLegend, 127606), Gr1 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated in 200 μl FACS buffer containing 1 μl Zombie NIR fixable dye for 30 min to determine cell viability (Zombie Green™ Fixable Viability Kit, BioLegend). Cells were fixed for 15 min in 200 μl PFA 2 % ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells transfected with pMAX-GFP and either pcDNA3.1-Rai or empty vector were added in a 10:1 ratio and incubated for 4 h in presence of APC anti-LAMP1 antibody (clone H4A3, Biolegend #328620). Monensin (2mM ...
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bioRxiv - Bioengineering 2023Quote: THP-1 cells were incubated with 2 µg/ml of anti-CD44 antibodies (clone: C44Mab-5; Biolegend, San Diego, CA, USA) for 20 minutes under standard cell culture conditions (95% humidity and 5% CO2 at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with primary anti-HA antibodies diluted in PBS-tween 0.2% blocking medium (mouse anti-HA, 1:5000, Biolegend cat. # 901513) over night at 4°C under agitation ...