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Citations for BioLegend :
8701 - 8750 of 8901 citations for DNAM 1 Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The sample pool was washed once with 10 mL of PBS-BSA and resuspended in 150 µL of Zombie NIR viability dye (1:500 in PBS; BioLegend 423105). After 15 minutes on ice ...
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bioRxiv - Cancer Biology 2023Quote: For inflammatory activation colon fibroblasts were plated in 6-wells and after 24 hours treated with 1 ng/ml murine recombinant IL-1α (Biolegend, 575002) in PBS for the indicated time periods ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were centrifuged at 1000 rpm and resuspended in flow buffer containing the primary antibodies: rat GFP (Biolegend, 338001, 1:200), guinea pig MAP2 (Synaptic Systems ...
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bioRxiv - Immunology 2023Quote: ... surface markers were stained with following antibodies at 1:200 dilution at 4°C for 30 min: CD11b- Pacific Blue (BioLegend, 101224), CD45-BV650 (BioLegend ...
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bioRxiv - Genetics 2023Quote: ... Recombinant Anti-AKT1 + AKT2 + AKT3 (phospho S472 + S473 + S474, Purified anti-Tubulin β-3 (TUBB3) Antibody 1:1000 (Biolegend, # PRB-435P), anti-GAPDH antibody 1:1000 (Sigma Aldrich ...
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bioRxiv - Bioengineering 2023Quote: THP-1 cells were incubated with 2 µg/ml of anti-CD44 antibodies (clone: C44Mab-5; Biolegend, San Diego, CA, USA) for 20 minutes under standard cell culture conditions (95% humidity and 5% CO2 at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with primary anti-HA antibodies diluted in PBS-tween 0.2% blocking medium (mouse anti-HA, 1:5000, Biolegend cat. # 901513) over night at 4°C under agitation ...
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bioRxiv - Neuroscience 2023Quote: ... non-fat dried skimmed milk powder and then probed with ICSM35 anti-PrP antibody (D-Gen Ltd; 1:5000 for RML) or with 6D11 anti-PrP antibody (BioLegend (808004); 1:5000 for ME7 ...
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bioRxiv - Cell Biology 2022Quote: ... The digested tissue was resuspended in 100 μL of 4% FBS in PBS containing an FITC antibody to CD11b (1:100; BioLegend, 101206) and PE antibody to CD45(1:100 ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... cells were resuspended in FACS buffer and incubated with Alexa Fluor® 488 anti-CD31 ([390] 102414) and APC/Cyanine7 anti-CD45 ([30-F11] 103116) antibodies (1/100, BioLegend) and mouse TruStain FcXTM (1/200 ...
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bioRxiv - Immunology 2023Quote: ... The following antibodies were used at a concentration of 1 µg/mL except where otherwise indicated: APC-Cy7-CD117 (clone 2B8; Biolegend #105826), PE-FceRI (clone MAR-1 ...
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bioRxiv - Immunology 2023Quote: ... we incubated sequential de-paraffinized sections with anti-2’,3’-cyclic-nucleotide 3’-phosphodiesterase (CNPase) (mouse CNPase: 1:200 dilution; Biolegend, Inc.), anti-CD3 (rabbit ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants were at 8 h post-infection and used fresh or stored at -20°C for up to 1 week and TNF was quantified using mouse TNF-α ELISA MAX kit (BioLegend) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Tyrosine hydroxylase was visualized using an Alexa Fluor® 594 conjugated mouse monoclonal anti-TH antibody at a 1:500 concentration (818004; Biolegend). Glutamine synthetase was visualized using a mouse monoclonal anti-GS antibody at a 1:100 concentration (MA5-27750 ...
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bioRxiv - Immunology 2023Quote: The following list of antibodies and their concentration against mouse proteins were employed: αCD45 (1:400; BioLegend Cat# 103147, RRID:AB 2564383), αCD3ε (1:300 ...
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bioRxiv - Cancer Biology 2023Quote: Cells were stimulated with IFNγ for 4h and the trypizinized cells were immunostained with VCAM1 antibody (1:100 dilution, BV421; BioLegend #305815). eBioscience™ Fixable Viability Dye eFluor™ 780 was used for discriminating dead cells ...
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bioRxiv - Bioengineering 2023Quote: ... Cells pre-treated with anti-mouse TfR-1 antibodies (human constant regions) were incubated with 50 nM of a second TfR-1 antibody for detection (R17, rat anti-mouse antibody; BioLegend, 113802). Cells pre-treated with anti-mouse CD98hc antibodies (human constant regions ...
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bioRxiv - Cancer Biology 2023Quote: ... single cell suspensions of samples incubated with myeloid antibody cocktail were washed and resuspended in PBS for flow cytometry run whereas lymphoid antibody cocktail-stained cells were incubated overnight with 1 mL perm buffer (Biolegend; 421402) containing 10 μL of anti-FoxP3 (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... cell suspensions were stained for 30 min at 4°C in the dark with CD45-BV605 (1:50, BioLegend #304042, RRID:AB_2562106), ITGA6-PeCy7 (1:100 ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were then incubated in 1×PBST with either rabbit anti-flagellin (kindly given by Dr Monica Rolando) or mouse anti-RNAPα (BioLegend #663104) diluted 1/5,000 for 1h at room temperature with agitation ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells transfected with pMAX-GFP and either pcDNA3.1-Rai or empty vector were added in a 10:1 ratio and incubated for 4 h in presence of APC anti-LAMP1 antibody (clone H4A3, Biolegend #328620). Monensin (2mM ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then stained with standard antibodies for immune and vascular lineage exclusion: anti-CD45-PE/Cy7 (BioLegend, 304016, 1:200), anti-CD31-PE/Cy7 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... and incubated in 100 μl of Maxpar Cell Staining Buffer with TruStain FcX™ (anti-mouse CD16/32) Antibody (1:100; 101320, Biolegend) for FC blocking (10 min ...
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Treg cells drive MYCN-mediated immunosuppression and tumor aggressiveness in high-risk neuroblastomabioRxiv - Cancer Biology 2023Quote: ... cells from both upper and lower chambers were collected and stained with BV421-conjugated CD4 (1:100, Clone RPA-TA, BioLegend, 300501) and APC/CY7-conjugated CD8 (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed 3 times for 5 minutes each with 1xPBS + .03% Triton before incubating for 2 hours in secondary solution containing 2% NDS + DAPI (1:1000; Biolegend 422801) + secondary antibodies (Alexafluor 647 donkey anti-chicken ...
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bioRxiv - Neuroscience 2023Quote: ... in phenol-red free DMEM-F12 in combination with live-dead stain Zombie NIR (1: 10,000 dilution; Biolegend, cat. no 423106) and incubated for 25 minutes at 37 °C and 5% CO2 conditions ...
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bioRxiv - Biochemistry 2023Quote: ... FoxP3-transduced CD4+ T cells were stained with antibodies targeting the cell surface antigens CD25 (Biolegend, Cat#102022, 1:1000 dilution) and Thy1.1 (Biolegend ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Following incubation on ice for 30 min., cells were pelleted, washed, and resuspended in PBS/BSA containing streptavidin-APC (1:2000 dilution in PBS/BSA, (BioLegend #405207)) and incubated for 30 min on ice prior to washing ...
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bioRxiv - Immunology 2023Quote: ... 6 x 106 cells were stained with the appropriate cocktail of cell-surface conjugated antibodies in 200µL FWB with the fixable viability dye Zombie Red (BioLegend; 1:1000) for 30 minutes on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... About 1*106 live cells were then blocked with TruStain FcXTM anti-mouse CD16/32 antibody (Clone 93; Biolegend; Cat #101320) on ice for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... and incubated in 100 μl of Cell Staining Buffer with TruStain FcX™ (anti-mouse CD16/32) Antibody (1:100; 101320, Biolegend) and True-Stain Monocyte Blocker™ (1:20 ...
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bioRxiv - Developmental Biology 2024Quote: ... or with an anti-integrin ß4 antibody coupled to R-phycoerythrin (ITGß4-PE, 1:500, clone 58XB4, BioLegend, San Diego CA) for isolation of CTB ...
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bioRxiv - Microbiology 2024Quote: ... each sample was counterstained with the desired concentration (0.5ul/1 × 106 cells) of 7AAD viability staining solution (Biolegend; Cat. No. 420404) before analysis ...
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bioRxiv - Microbiology 2024Quote: ... Cells were pelleted by centrifugation (2,000 rpm 5 min) and then treated with 1 mL 1X red blood cell (RBC) Lysis Buffer (Biolegend; cat# NC9067514) to lyse red blood cells ...
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bioRxiv - Cell Biology 2024Quote: ... Cell pellets were gently resuspended in 100 µl FACS buffer and stained with antibodies (CD38-APC, Clone: HIT2 (Biolegend, 1:100), CD45RA-PE-Cy7 ...
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bioRxiv - Cancer Biology 2024Quote: ... single cells isolated from tissue were labeled for 30 min at room temperature with Alexa Fluor 647-conjugated anti-EpCAM (1:50, Biolegend 324212), PE-conjugated anti-CD49f (1:100 ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were permeabilized for intra-cellular staining with a saponin-based buffer (1% saponin, 10% BSA in PBS) and stained with an antibody for Cytochrome C AlexaFluor 647 (Biolegend, 612310) used at 1:2000 dilution and DAPI ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then stained with the following conjugated cell-surface marker antibodies at 1:50 dilutions: CD326 (EpCAM) PE (Biolegend, 324206) and CD45 BV786 (Biolegend ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were permeabilized for intra-cellular staining with a saponin-based buffer (1% saponin, 10% BSA in PBS) and stained with an antibody for Cytochrome C AlexaFluor 647 (Biolegend, 612310) used at 1:2000 dilution and DAPI ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were washed with DPBS three times and stained with 1 µg/mL Streptavidin-Alexa Fluor 647 (AF647) (BioLegend, #405237) for another 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... 0.3% triton in PBS) for 3 hours then incubated with Alexa Fluor 594 anti-β-amyloid (Clone 6E10; 1:1,000; BioLegend; 803019), Olig2 (1:500 ...
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bioRxiv - Immunology 2024Quote: ... diluted 1:500 in 1x Permeabilisation buffer with 5% Normal Donkey Serum at +40C and 1 h staining with DyLightTM 649 Donkey anti-rabbit IgG (BioLegend, #406406) diluted 1:500 in 1x Permeabilisation buffer ...
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bioRxiv - Cell Biology 2024Quote: ... The live mCherry+ or mCherry- Epcam+ miPSC or GFP+ hiPSC were sorted using the following staining: anti-Epcam antibody (1:50) (Biolegend; 118225). Cells were loaded on a 10x Genomics Chromium controller.
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bioRxiv - Molecular Biology 2024Quote: ... and incubated for 25 m on ice in a cocktail of fluorochrome-conjugated antibodies (B220 (RA3-6B2-BV605; 1:200; BioLegend #103244), TCRβ (H57-597-PE-Cy7 ...
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bioRxiv - Cell Biology 2020Quote: ... Around 900 μl of plasma from each mouse was used to stain for T cell (1:150, APC-CD3e/clone 145-2C11, Biolegend Cat. 100312), B cell (1:300 ...
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bioRxiv - Immunology 2021Quote: ... clone: NKI-SAM-1), αL (CD11a, clone: HI111), αM (CD11b, clone: M1/70), αX (CD11c, clone: 3.9) (all Biolegend, San Diego, CA), or α9 (CD49i ...
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bioRxiv - Immunology 2021Quote: ... five groups of cells were pre-labelled with mouse HashTag oligos (HTO) on cell surface: spleen T cell control group (SPT ctrl) with hashtag 1 (BioLegend, Cat# 155801); spleen T cell IL-1β/IL-23 stimulation group (SPT stim ...
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bioRxiv - Immunology 2019Quote: ... against intracellular antigens of interest were added and incubated for 90 minutes at 4°C (IFNγ: PE-Cy7, clone XMG1.2; IL2: PE, clone JES6-5H4; TNFα: APC, clone MP6-XT22; all Biolegend. all 1:200). Next ...
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bioRxiv - Immunology 2019Quote: PBMCs were seeded in a 24 well plate at 2 × 106 cells/well in RPMI-1640 with anti-CD107a antibody (Biolegend, 1:200). Then ...
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bioRxiv - Neuroscience 2021Quote: Nrxn1 neuron cKO-T brain slices (coronal and sagittal) were labeled for Nrxn1 (Fig. 1c-d) using purified HA-647 (mouse, 1:500, Biolegend, Cat# 682404) as described above ...