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Citations for BioLegend :
8501 - 8550 of 8901 citations for DNAM 1 Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 50,000 CD4+ T cells were added to each well of mBECs in 12-well chamber slides in Hank’s balanced salt solution (HBSS) with 1% FBS supplemented with 5 nM CXCL10 (Biolegend 573604) or vehicle (PBS) ...
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bioRxiv - Immunology 2022Quote: ... The permeabilised cells were then intracellularly stained for 1 hour at room temperature (RT) with anti-STAT6Tyr641 Alexa Fluor 647 (A15137E; Biolegend) and anti-NFκB p65Ser536 Alexa Fluor 488 (93H1 ...
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bioRxiv - Genetics 2022Quote: ... For mtscATAC-seq and ASAP-seq experiments residual granulocytes were excluded by staining of cells using 1:50 PE-conjugated CD66b (clone G10F5, 305102, BioLegend). For live/ dead cell discrimination Sytox Blue was used at a 1:1000 dilution according to the manufacturer’s instructions (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T-cells were cultured alone or co-cultured with either of the B-cell populations at a ratio of 3:1 for 60 h at 37°C and 5% CO2 on anti-CD3 (2 μg/mL) (ULTRA-LEAF Biolegend) coated flat bottomed 384 CellCarrier Ultra microplates (PerkinElmer ...
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bioRxiv - Neuroscience 2022Quote: ... Retinas were then placed in primary antibody solution containing mouse anti-SMI-32 (1:1000, BioLegend, Cat#801701, RRID: AB_509997) in blocking solution for 2-4 days at 4°C ...
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bioRxiv - Pathology 2022Quote: ... The pellet was further washed once with FACS buffer (0.5% BSA/0.5 mM EDTA in PBS) and stained with APC-conjugated anti-mouse CD45 (1:300, BioLegend, CAT#103112), BV510-conjugated anti-mouse CD11b (1:300 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated overnight at 4°C with primary antibodies diluted in blocking solution: anti-βIII Tubulin (1/500, Biolegend), anti-RSK2 (1/100 ...
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bioRxiv - Immunology 2022Quote: ... Samples were then stained for extracellular markers in the presence of Fc block for 30 minutes at 4°C protected from light using the following antibodies: CD3 (APC/Cy7; 1:300; clone 17A2; BioLegend), CD4 (AF488 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and at passage 1 a depletion of CD45+ cells was performed using a Biotin anti-mouse CD45 Antibody (Biolegend, 103104) and Dynabeads™ Biotin Binder (ThermoFisher 11047) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were restimulated at 1×106 cells/mL concentration for 48 h on day 8-10 with 1 µg/mL plate-bound anti-CD3ε antibody (clone UCHT1, Biolegend). BiTE-mediated killing assays were carried out on day 14-16 of culture.
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bioRxiv - Cell Biology 2022Quote: ... were performed after confirming full differentiation into mature MCs using flow cytometric analysis of lineage-specific c-KIT (rat anti-CD117 Brilliant Violet 421-conjugated, 1:200, Biolegend) and FcεRI (armenian hamster anti-FcεRIα Alexa Fluor 647-conjugated ...
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bioRxiv - Cell Biology 2022Quote: ... Full differentiation into mature MCs was confirmed by expression of lineage-specific c-KIT (rat anti-CD117 Brilliant Violet 421- conjugated, 1:200, Biolegend) and FcεRI (armenian hamster anti-FcεRIα Alexa Fluor 647- conjugated ...
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bioRxiv - Cell Biology 2024Quote: ... Cells from the epithelial layer were sorted gating on single positive for CD326-PE/Cy7 (BioLegend, clone DECMA-1, #147310) and CD104-AF647 (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... PL1 or KPL1 antibodies were incubated for 1 h followed by PE conjugated goat anti-mouse secondary antibody (cat. no. 405307, Biolegend) for another hour ...
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bioRxiv - Bioengineering 2024Quote: ... Biotinylated rasburicase was reacted for 20 min on ice with 4:1 molar ratio of biotin to streptavidin-conjugated PE or streptavidin-conjugated APC (Biolegend). Streptavidin-conjugated FITC (BioLegend ...
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bioRxiv - Genomics 2024Quote: ... amplification and sequencing libraries were generated using the Next GEM Single Cell 3’ Kit v3.1 (10X Genomics) with the recommended modifications for compatibility with the TotalSeq-A cell hashing and CITE-seq reagents (BioLegend). Organ donor GEX libraries were sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... Mouse lung single cells were similarly stained but with a cocktail of antibodies (1:250 each) against CD45-PE/Cy7 (103114, BioLegend), Icam2-A647 (A15452 ...
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bioRxiv - Neuroscience 2024Quote: ... FluoSphere- containing media was then removed from the wells and cells were processed for flow cytometry using markers for CD11b (1:200, 101206, Biolegend) and CD45 (1:200,103132 ...
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bioRxiv - Immunology 2024Quote: ... FL or bone marrow HSPC were cultured on OP9-DL1 cells in the presence of 1 ng/mL IL-7 and 5 ng/mL FLT3L (BioLegend) as previously described 70 ...
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bioRxiv - Immunology 2023Quote: ... samples from animals that were vaccinated with the recombinant GPCysR4-I53-50A.30 To exclude I53-50A-binding B cells we included a SARS-CoV-1 Spike probe containing I53-50A-biotin which was labeled with streptavidin AF647 and streptavidin BV421 (both Biolegend). After a 2 day culture with interleukin (IL)-21 and irradiated mouse fibroblast (L cells ...
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bioRxiv - Neuroscience 2024Quote: ... and heavy non-phosphorylated neurofilament (NF-H) enriched in large pyramidal neurons (Anti-NF-H mouse monoclonal antibody, clone SMI32, 1:2000, Biolegend). All sections were counterstained with hematoxylin for further visualization of cell organization used in cytoarchitectonic type identification explained below and in Supplementary Table 1.
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bioRxiv - Immunology 2024Quote: ... were cultured for 3 days in 96-well U bottom plates coated with 2 μg/mL anti-CD3 (eBioscience) and 1 μg/mL anti-CD28 (eBioscience) supplemented with 10ng/mL of recombinant mIL-2 (Biolegend) to allow Treg survival ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were immunolabeled in blocking solution for 1 hour at RT with antibodies against the following proteins: ALIX at 5 μg/ml (mouse; no. 634502, Biolegend); IST1 at 0.4 μg/ml (rabbit ...
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bioRxiv - Immunology 2024Quote: ... in the presence of S2 mAbs (5 μg/mL), protein transport inhibitor cocktail (1:500, eBioscience) and anti-CD107a APC (clone H4A3, BioLegend). Antibody-dependent NK cell activation was allowed to occur for 4 hours at 37°C and 5% CO2 ...
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bioRxiv - Developmental Biology 2024Quote: ... and the resulting cDNA was amplified using as PCR oligos the partial TruSeq read 1 adaptor (provided in the 10x kit) and an additive primer (a partial TruSeq read 2, sequence provided by Biolegend and synthesized by Integrated DNA Technologies) ...
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bioRxiv - Immunology 2024Quote: ... Wells were washed 5 times with 200 µL PBST and streptavidin-conjugated to horseradish peroxidase was added (1:2000 in PBS, BioLegend) for 30 minutes at RT ...
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bioRxiv - Immunology 2024Quote: ... plates were coated per well with 100 µL of anti-IgE capture antibody (2 µg/mL in PBS, clone RME-1, BioLegend) overnight at 4⁰ C ...
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bioRxiv - Immunology 2024Quote: ... the cells were stained with a live/dead fixable dye (Zombie Red dye, 1/800 dilution – BioLegend, San Diego, CA) for 20 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... which were obtained from indicated sources: APC anti-mouse I-A/I-E (MHC II) (clone M5/114.15.2, BioLegend, 1:500), FITC anti-mouse I-A/I-E (MHC II ...
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bioRxiv - Immunology 2024Quote: ... The following antibodies were used for staining: Alexa Fluor 488 anti-mouse CD3 (17A2) (1:100) (BioLegend, San Diego, CA), PE anti-mouse CD4 (GK1.5 ...
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bioRxiv - Immunology 2024Quote: γδ IELs were cultured ex vivo as previously described (33) in which 100,000 γδ IELs were activated with 1 ÿg/mL anti-CD3 (2C11, BioLegend) in the presence of 10 U/mL IL-2 ...
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bioRxiv - Immunology 2024Quote: ... and anti-dsDNA antibodies were detected with a 1/1000 dilution of alkaline phosphatase–conjugated goat anti-mouse IgG (BioLegend) for 1 hour at 37°C and developed with a phosphatase substrate for 30 min at 37°C.
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bioRxiv - Immunology 2024Quote: ... and stained with mouse anti-human HLA-E primary antibody or mouse anti-human HLA class I primary antibody (clone W6/32, purified from hybridoma cells) in 1:100 dilution with cell staining buffer (BioLegend) and a secondary antibody goat anti-mouse antibody conjugated with APC or rat anti-mouse antibody conjugated with Alexa Fluro 700 (BioLegend) ...
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bioRxiv - Microbiology 2024Quote: ... parasites were washed twice with FACS buffer and stained with (1:100) goat anti-mouse IgG-APC (BioLegend, cat# Poly4053). After incubation ...
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bioRxiv - Genetics 2023Quote: ... Lin-Sca-1+ c-Kit+ Flt3-CD150+ CD48-on a FACSAria into StemSpan SFEM II media with SCF (100 ng/ml, BioLegend), TPO (50 ng/ml ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit) at 1:100 dilution (for immunodepletion) or 3 μg/ml of each 4G8 and 6E10 anti-Aβ antibodies (Biolegend) (for brain-derived Aβ enrichment ...
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bioRxiv - Pathology 2023Quote: ... Single cell suspension was stained with antiCD45:Pacific blue (368539, clone 2D1, Biolegend, San Diego, CA, USA, 1:200 dilution), anti-EpCAM:BV650 (324225 ...
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bioRxiv - Immunology 2023Quote: ... Intracellular cytokine stain was performed following restimulation of pmel-1 T cells as above with the addition of 1X Brefeldin A (Biolegend) and 1X Monensin (Biolegend ...
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bioRxiv - Microbiology 2023Quote: ... for 30 minutes at 37°C and then incubated with 10 µg/ml of αASC TMS-1 (clone HASC-71, Biolegend) in blocking buffer ...
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bioRxiv - Immunology 2022Quote: T cells were adjusted at a concentration of 1 × 106 cells/100 μl CTL medium including Golgi-Stop (Biolegend, 420701) and Golgi-Plug (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... Following combinations of cytokines and antibodies were used for the differentiation of T cells: for Th1 cells - 1 µg/ml anti-IL-4 (clone 11B11; Biolegend), 30 U/ml rhIL-2 (NIH) ...
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bioRxiv - Immunology 2023Quote: ... 100 uL/well) were restimulated with coated anti-CD3 antibody (1 ug/mL, 100 uL/well, 4 °C overnight, Clone: OKT3, Biolegend) and soluble anti-CD28 antibody (2 ug/mL ...
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bioRxiv - Microbiology 2023Quote: ... percoll isolated cells were activated for 5 hours at 37 °C using a Cell Activation Cocktail (1:500, Biolegend, USA). The cells were washed with PBS once and stained with surface staining antibody for 40min at 4 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... An aliquot of the resulting suspensions in PBS were immediately stained for viability with Zombie Aqua diluted 1:1000 (BioLegend) for 20 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Sip-T or single cell suspensions of tumor-infiltrating lymphocytes (TILs) were resuspended in PBS as 1-3×106 cells/ml and stained with fixable Zombie Aqua viability dye (BioLegend) as per manufacturer’s recommendation ...
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bioRxiv - Microbiology 2023Quote: ... Adjacent sections were deparaffinized in citrate and stained with monoclonal mouse anti-F4/80 antibody at a 1:400 dilution (BioLegend), or polyclonal goat anti-MPO antibody at a 1:500 dilution (R&D Systems) ...
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bioRxiv - Cancer Biology 2023Quote: ... Wells of a 24-well plate were coated with 1 μg/mL anti-CD3 (#317326) and anti-CD28 antibodies (#302934, both BioLegend). For activation of T-cells ...
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bioRxiv - Immunology 2023Quote: ... the slides were incubated for 1 hour in the dark at room temperature with an Alexa 488-conjugated goat anti-mouse IgG antibody (BioLegend). Following three washing steps ...
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bioRxiv - Developmental Biology 2023Quote: ... When staining for SLAM markers the same lineage cocktail was used (FITC) with the addition of the following antibodies: anti-CD48 (FITC, clone HM48-1, BioLegend), anti-CD41 (FITC ...
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bioRxiv - Immunology 2023Quote: ... Pacific Blue (or v450), and APC-Cy7 (or Alexa Fluor-e780)-conjugated antibodies (1:100, CD4, CD8) purchased from Biolegend. After cell surface marker staining ...