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Citations for BioLegend :
8601 - 8650 of 8901 citations for DNAM 1 Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Surface staining of the Macrophage panel consisted of the following primary antibodies diluted in FACS buffer (2% FBS in DPBS) with anti-mouse CD45-Brilliant Violet 650 (1:200, 103151, Biolegend), anti-mouse F4/80-Brilliant Violet 785 (1:200 ...
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bioRxiv - Immunology 2024Quote: ... The cell suspension was stained using Brilliant Violet 785™ anti-mouse CD45 antibody (1:100, Biolegend, Clone 30-F11), Brilliant Violet 421™ anti-mouse F4/80 antibody (1:100 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were stimulated with anti-CD3/CD28 (3.5 µg/ml anti-CD3 mAb, BioLegend, #100339; 1 µg/ml anti-CD28 mAb, BioLegend, #102115) in the presence of 5 μg/mL Brefeldin A (BFA ...
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bioRxiv - Cancer Biology 2024Quote: ... The viability of T-ALL#1 cell suspension was assessed by flow cytometry with Zombie Aqua Viable dye (423102, BioLegend) prior to mouse injection ...
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bioRxiv - Immunology 2024Quote: ... 0.5-1×106 cells were resuspended in 50 μL/well T cell stimulation media (5 μg/mL anti-mouse CD28 (Biolegend), 5 ug/mL BFA) ...
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bioRxiv - Immunology 2024Quote: ... cells were centrifuged at 500 xg for 7 minutes and resuspended in 1 mL ice cold Cell Stain Buffer (CSB, BioLegend). Samples were filtered through a sterile pre-wet 70 μm mesh ...
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bioRxiv - Immunology 2024Quote: ... Cellular debris and red blood cells were removed by the Debris Removal Solution (Miltenyi) and 1× RBC Lysis Buffer (Biolegend), respectively ...
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bioRxiv - Microbiology 2024Quote: ... LAMP-1 was stained as described previously for LAMP-2 but using a rat-IgG anti-mouse LAMP-1 and anti-rat-IgG-AlexaFluor647 (Biolegend) [56] ...
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bioRxiv - Developmental Biology 2024Quote: ... Single-cell suspensions were generated as described above with an additional incubation with anti-CD11b-BV421 (1:200, Biolegend #101251) and anti-Ly6g-AF647 (1:200 ...
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bioRxiv - Immunology 2024Quote: ... in the presence of anti-Ly6G AF488 (1A8) and anti-CD68 AF647 (FA-11) or their isotypes (1/100 each, Biolegend), and counterstained using 500ng/mL DAPI dilactate (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... peptide-specific T cells were evaluated by FCM by staining with PE-conjugated MART-1(A27L) tetramer and anti-CD8 FITC (BioLegend) (Supplementary Table 1).
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bioRxiv - Cell Biology 2020Quote: ... Staining of the red blood cell membrane was performed with the anti-Ter119::Alexa647 antibody (Biolegend, 0.5 mg/ml; 1/1000) for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... Slides were incubated with the following primary antibodies diluted in blocking buffer overnight at 4°C – chicken anti-keratin 14 (1:800, Biolegend 906001), rabbit anti-SCD1 (1:500 ...
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bioRxiv - Immunology 2021Quote: ... and cells were stained with 1.5 μg/ml Brilliant Violet 605™ anti-CD16 (clone: 3G8) and 1 μg/ml APC anti-CD18 (clone: 1B4/CD18) (Biolegend). Cell data was collected using an Attune flow cytometer (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... ENR media was supplemented with 500 ng/ml rAspn and 1 μg/ml of ultra-LEAF™ CD44 function blocking antibody (Clone IM7; Biolegend) or equal amount of isotype control IgG2b kappa (Clone RTK4533 ...
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bioRxiv - Immunology 2022Quote: ... 50 μl of supernatants from cultures of T cell clones were collected after 48 hrs of stimulation and added to each well of 96-well round-bottom plates precoated with IFN-γ (clone MD-1) capturing antibodies (BioLegend). After overnight incubation ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated with the following primary antibodies overnight at 4 : mouse anti-C3 (1:200 dilution; Biolegend cat. no. 846302) + rabbit anti-GFAP (1:500 dilutio L gilent Dako cat ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were blocked using PBS/1% BSA/0.5% Tween-20 and incubated with monoclonal mouse FITC anti BrdU (Biolegend, clone 3D4) at 1:50 dilution for 20 minutes ...
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bioRxiv - Immunology 2021Quote: Naïve CD8+ T cells (OT-1 or Pmel-1) were isolated from pooled spleen and lymph nodes using MojoSort™ mouse CD8 T cell isolation kit (Biolegend) following manufacturer’s instruction ...
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bioRxiv - Genomics 2019Quote: ... Isolated nuclei were labeled with an Alexa Fluor 647 anti-GFP antibody (338006, clone FM264G, 1:25, BioLegend, San Diego, CA) for 30 minutes and 2 μg/ml DAPI immediately prior to sorting ...
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bioRxiv - Immunology 2019Quote: ... The cells were incubated with the following antibodies (1:100) for 30 minutes at room temperature: Alexa Fluor 488 anti-mouse CD25 (Biolegend, 102017), PE/Cy7 anti-mouse/human CD44 (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... The following antibodies were added (1:100) to each sample and incubated for 30 minutes at room temperature Alexa Fluor 700 anti-mouse CD8a (Biolegend, 100730), PE/Dazzle-594 anti-mouse CD4 (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... anti-CD3 (clone OKT3, BV650), anti-CD4 (clone OKT4, BV785), anti-PD-1 (EH122.2H7, BV711), anti-CD27 (O323, BV605) from Biolegend (London, UK); anti-CD45 (clone 2D1 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... This was followed by an incubation period with Anti-Aβ mouse monoclonal antibody 6E10 (1:1000, epitope: Aβ3–8, Biolegend, San Diego) and peroxidase-conjugated anti-mouse IgG (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... This was followed by an incubation period with Anti-Aβ mouse monoclonal antibody 6E10 (1:1000, epitope: Aβ3-8, Biolegend, San Diego) and peroxidase-conjugated anti-mouse IgG (1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... or double knockout cells were harvested and washed with PBS containing 1% bovine serum albumin (BSA) and incubated with 5 μL of PE-conjugated EphA2 antibody (Biolegend, SHM16) in 50 μL PBS containing 1% BSA for 30 minutes at 4 °C ...
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bioRxiv - Immunology 2019Quote: ... 105 resting NK cells were mixed at 1:1 with 221 cell lines or peptide loaded target cells in presence of 1 μl BV421 anti-CD107a mAb for 2 hr at 37°C (H4A3, Biolegend 328626). NK cells were then stained with mAbs to identify subsets based on the expression of KIR2DL1/S1 (Beckman Coulter ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed twice and stained in 100 μl for 30 minutes on ice using an anti-mouse IgG-Brilliant Violet421 secondary antibody at 1:90 (BioLegend, 405317). Cells were again washed twice ...
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bioRxiv - Immunology 2021Quote: ... Samples were then washed twice with 1 mL of Intracellular Staining Perm Wash buffer (Cat: 421002, BioLegend, San Diego, CA, USA); after the second wash ...
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bioRxiv - Cell Biology 2021Quote: ... The fixed cells are permeabilized with 0.1% Triton-X 100 for 10min at RT and stained with anti-vimentin (1:200, Biolegend, CA, USA) for 30 min in PBS containing 5% normal goat serum (RT) ...
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bioRxiv - Bioengineering 2020Quote: ... Antigen-independent activation-marker expression of CAR-T cells was evaluated by antibody staining for CD137 (PE/Cy7, clone 4B4-1, BioLegend #309818) and CTLA-4 (PE/Cy7 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell sorting was repeated on cultured CAFs and PVL cells using the previously described experimental conditions with anti-PDGFRα (1:100; BioLegend #323507), anti-CD146 (1:100 ...
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bioRxiv - Microbiology 2020Quote: ... mouse monoclonal anti-DnaK 4RA2 at 1□:□10,□000 dilution (Enzo, ADI-SPA-880) and HRP Goat anti-mouse IgG (BioLegend, 405306) at 1□:□10,□000 dilution ...
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bioRxiv - Bioengineering 2021Quote: ... PBMCs were collected and stained using APC/Fire anti-CD4 and APC anti-CD8 (Supplemental Table 1) (Biolegend, San Diego CA). PBMCs were first washed and stained with Zombie Yellow (Supplemental Table 1 ...
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bioRxiv - Cell Biology 2021Quote: ... CD59 (PE, 143103), PD-1 (BV605, 135220), CD44 (BV711, 103057), CD69 (PE-Cy7, 104512), IFN-γ (APC, 505810) (all from Biolegend). The Foxp3 Fix/Perm Kit was used for intracellular staining (421403 ...
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bioRxiv - Microbiology 2021Quote: We performed western blotting as in (Polino et al., 2020) with primary antibodies mouse anti-HA diluted 1:1000 (clone 16B12; Biolegend #901501), rabbit anti-PfAldolase diluted 1:2000 (abcam #ab207494 ...
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bioRxiv - Developmental Biology 2021Quote: ... Blocking of Fc receptors was performed by incubation at 4°C for 20 minutes with an unlabelled anti-CD16/32 (1 μg/million cells; BioLegend – 101320). The cells were then incubated for one hour at 4°C in the dark with a mix of rat anti-mouse CD41 (labelled with Phycoerythrin ...
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bioRxiv - Systems Biology 2021Quote: Single nuclei from each PDX condition were labeled with 1 μl condition-specific hashtag oligonucleotide-labeled antibodies (BioLegend, TotalSeq A0541-A0545) according to manufacturer’s protocol prior to pooling and loading on a single lane of the 10X Chromium v2 system ...
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bioRxiv - Microbiology 2019Quote: ... Cells were harvested using Trypsin-EDTA and resuspended in FACS buffer (PBS containing 10% FBS and 2mM EDTA) and stained with anti-CD300lf PE clone Tx70 (1:100; BioLegend, #132704) or anti-CD300ld PE clone Tx69 (1:100 ...
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bioRxiv - Cancer Biology 2020Quote: ... VDR overexpressing SKOV-3 and ECC-1 cells were processed and stained with PDL1-BV605 antibody (Biolegend Inc, Catalog number:124321). VDR expressing SKOV-3 and ECC-1 cells ...
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bioRxiv - Cell Biology 2021Quote: ... non-adherent cells were moved to bacteriological Petri dishes the next day and differentiated over 1 week in cDMEM containing 20 ng/mL recombinant murine M-CSF (Biolegend, 576404). Generation of murine bone-marrow derived dendritic cells (BMDC ...
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bioRxiv - Cell Biology 2021Quote: ... Mac-1-PE-Cy7 and FcγR-PE (eBioscience, 12-0161-83) for myeloid differentiation, or Mac-1-APC (eBioscience, 17-0112-82) and CD19-PB (BioLegend, 115523) for lymphoid differentiation on OP9 cells ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was removed and approximately 106 cells were resuspended in FACS buffer containing an anti-HA AF488 conjugate (1:1000, BioLegend, 901509) and an eFluor 780 fixable viability dye (Thermo Fischer Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed once in Cyto-Fast Wash Buffer and incubated in Cyto-Fast Wash Buffer containing an anti-HA AF647 conjugate (1:1000, BioLegend, 682404) and an anti-FLAG AF549 conjugate (for cells expressing FLAG-tagged M ...
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bioRxiv - Molecular Biology 2022Quote: ... Whole-lung suspension was then repelleted and resuspended in SB + 1:50 TruStain FcX™ antibody (anti-mouse CD16/32, BioLegend) for a 10-minute blocking period at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies used for removal of mouse stromal cells included: anti-mouse H-2Kd-biotin (BioLegend, 116604, clone SF1.1, dilution 1:20) and anti-mouse Cd45-PE/Cyanine5 (BioLegend 103110 ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-1 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40634-V27H-B) conjugated with PE-streptavidin (BioLegend, 405204), 0.0032 μg SARS-CoV-2 biotinylated RBD protein (His & AVI Tag ...
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bioRxiv - Immunology 2022Quote: ... and then stained at room temperature for 15 min with a 1:200 dilution of the LIVE/DEAD Zombie Aqua™ Fixable Viability Kit (Biolegend) reagent to exclude dead cells ...
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bioRxiv - Cancer Biology 2022Quote: The following primary antibodies with indicated dilution in blocking buffer were used: Rabbit anti-Tuj1 (Covance, BioLegend, MRB-435P, 1:1,000), Mouse anti-Tuj1 (Covance ...
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bioRxiv - Neuroscience 2020Quote: ... The following antibodies were then added to cell suspensions at 1:200 concentrations: CD11b-PE (BioLegend Cat#: 101208; Clone: M1/70), CD45-APC/Cy7 (BioLegend Cat# ...