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Citations for BioLegend :
8251 - 8300 of 8901 citations for DNAM 1 Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... mouse monoclonal anti-Aβ (anti-β-amyloid 17-24, clone 4G8, #800712, 1:500, Biolegend, San Diego, CA, USA) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... The following antibodies were used for flow cytometry and stimulation: FITC-labelled anti-HLA-A2 (BB7.2, Biolegend, 1:40), AlexaFluor® 647-labeled anti-HLA-A,B,C (W6/32 ...
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bioRxiv - Cell Biology 2019Quote: ... with 2□mM EDTA for flow analysis or resuspended in PBS (pH 7.2) with 2□mM EDTA and 1□μg/ml DAPI (Biolegend) for sorting ...
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bioRxiv - Microbiology 2020Quote: ... 1;100 dilution) and an antibody against SR-B1 for FACS (363208, clone m1B9; dilution 1:100) were from Biolegend. The SARS-2-S mAb (GTX632604 ...
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bioRxiv - Microbiology 2021Quote: ... in PBS containing the primary antibodies at a 1:100 dilution: mouse anti-DC/L-SIGN (Biolegend, cat. 845002), rabbit anti-DC-SIGN (Cell Signaling ...
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bioRxiv - Neuroscience 2019Quote: ... in Alexa Fluor® 488) and rat anti-mouse CD31 (1:100; Biolegend 390 in APC/Fire™ 750) for 30 minutes at 4°C ...
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bioRxiv - Genomics 2021Quote: ... and then incubated 1:100 with antibodies directed against cell surface proteins CD11b-BV421 (BioLegend 101251, clone M1/70), CD45-APC (BioLegend 103112 ...
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bioRxiv - Immunology 2020Quote: ... and Tregs (anti-CD3-BV510, anti-CD4-PE, anti-CD8-AF700, anti-CD25-APC (1:100, Biolegend, Cat#302610), anti-FoxP3-AF488 (1:100 ...
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bioRxiv - Immunology 2020Quote: ... Th-17 (anti-CD3-BV510, anti-CD4-PE, anti-CD8-AF700, anti-IL17A-APC (1:100, Biolegend, Cat#512334)) and Tregs (anti-CD3-BV510 ...
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bioRxiv - Immunology 2019Quote: ... The cell pellet was then resuspended in 50µl (1/50 dilution in FACS buffer) of TruStain FcX blocker antibody (BioLegend) and incubated at 4°C for 10minutes ...
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bioRxiv - Immunology 2020Quote: ... and then plated into 12 well plates previously coated for 2 hours RT with 1 μg/ml of huCD28.2 (BioLegend) and 1 μg/ml of human CD3ε (BioLegend) ...
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bioRxiv - Neuroscience 2021Quote: ... The cell pellet was then stained with antibodies to microglial cell surface markers (CD11b-PE Cy7, 1:200 Biolegend, # 101216 ...
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bioRxiv - Cancer Biology 2020Quote: ... and F480 (BM8, #123114), Gr-1 (clone RB6-8C5, #108428), Ly6G (Clone 1A8; #127645) and Ly6C (Clone HK1.4, #128030) from BioLegend. After 30 minutes of staining ...
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bioRxiv - Systems Biology 2021Quote: ... and then stained the cells for 30 min with 1:500 anti-NGFR APC-labeled clone ME20.4 (BioLegend, 345107). We washed the cells five times with 0.1% BSA/PBS and followed with one final wash with PBS for 2 min at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... single cell suspensions were resuspended in FACS buffer (PBS, 2%FBS, 0.01%Tween) and stained for viability dye (1/1000, Biolegend) in 100ul PBS for 10min at RT followed by surface staining with antibody specific for CD45 (30-F11 ...
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bioRxiv - Genomics 2019Quote: ... equal amounts of H1 and HEK293T cells were mixed and stained with anti-TRA-1-60 (Biolegend, Cat#330610) antibody.
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bioRxiv - Immunology 2021Quote: ... and seeded in the antibody-coated flask at 3–5×105 cells/cm2 together with soluble 1 µg/mL anti-CD28 antibodies (clone 37.51; 102116, BioLegend). Cells were cultured for 48 hr at 37°C in a humidified 5% CO2 atmosphere ...
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bioRxiv - Immunology 2020Quote: ... Cells were then stained for surface markers followed by intracellular cytokines using antibodies specific for IFN-y (4S.B3, 1:40) (Biolegend) IL-2 (MQ1-17H12 ...
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bioRxiv - Immunology 2020Quote: ... and incubated with biotin conjugated anti-CD8 (53-6.7) and anti-CD19 (6D5) at a dilution of 1:200 (both from Biolegend) and 50μL/mL normal rat serum for 10 min at RT ...
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bioRxiv - Immunology 2020Quote: ... membranes were incubated with primary antibodies for 1h at RT with shaking (1:2000 anti-filaggrin clone Poly19058, Biolegend; 1:2000 anti-β-actin ...
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bioRxiv - Molecular Biology 2020Quote: ... The following primary antibodies were used at the indicated concentrations: mouse anti-HA (1:200, HA.11 901501 Biolegend), mouse anti-BRCA1 D-9 (1:40 ...
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bioRxiv - Cell Biology 2020Quote: ... T- and B-cell depleted cultures were incubated with antibodies for CD34 (clone 561, PE conjugate, BioLegend, 1:100) prior to sorting ...
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bioRxiv - Immunology 2021Quote: ... with the caveat that CD8+ enrichment was achieved by incubating 1-2 million PBMCs with CD8 Mojobeads (Biolegend 480108) for 15 minutes on ice ...
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bioRxiv - Molecular Biology 2020Quote: ... and c-Kit on mast cells were analyzed by Flow cytometry using fluorochrome labelled antibodies (PE-CY7–conjugated anti-FcεRIα, MAR-1 Biolegend #134317 ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-1 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40634-V27H-B) conjugated to PE-streptavidin (BioLegend, 405204), SARS-CoV-2 biotinylated RBD protein (His & AVI Tag ...
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Bacterial Polyphosphates Induce CXCL4 and Synergize with Complement Anaphylatoxin C5a in Lung InjurybioRxiv - Immunology 2022Quote: ... preincubated for 20 min with anti-mouse CD16/CD32 antibody (TruStain FcX, BioLegend, San Diego, CA, USA, 1:50) in FACS buffer followed by 20 min incubation with fluorescent dye-conjugated ...
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bioRxiv - Cell Biology 2022Quote: ... poly-l-lysine coated glass slides were coated by overnight incubation with ICAM-1 (5 μg/mL, Biolegend 552906) or with ICAM-1 and anti-CD28 (5 μg/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... Either TotalSeq-A Anti-Nuclei Pore Complex Proteins Hashtag 9 or 10 Antibody (1uL at 1:5 dilution; BioLegend) was added to each aliquot of lysis buffer ...
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bioRxiv - Immunology 2022Quote: ... enriched CD8 T cells were incubated for 10’ at 4°C in Staining buffer (2%BSA/0.01% Tween in PBS) containing FcBlock (1/10 dilution ;TruStainTM FcX, Biolegend) followed by a 30’ incubation in Staining Buffer containing TotalSeqB antibodies against Ly108 ...
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bioRxiv - Immunology 2022Quote: ... and then stained for surface antibodies at .1 μg/mL unless indicated: NK1.1 PerCP-Cy5.5 (clone PK136, BioLegend 10727), F4/80 AF594 (clone BM8 ...
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bioRxiv - Pathology 2022Quote: ... The following antibodies were used at 1:200 unless specified: anti-CD9 (clone MZ3, FITC, APC/Fire750, biotin; BioLegend), anti-CD31 (clone 390 ...
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bioRxiv - Neuroscience 2023Quote: ... cat no: 130-123-284) and allophycocyanin (APC) conjugated rat anti-mouse CD45 (1:100, Biolegend, cat no: 103111) in blocking solution for 30 minutes on ice in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... For further experiments viable CD45highCD8+ cells were labeled with rat anti-CD45 APC (1:100, catalog no. 103111; BioLegend), rat anti-CD8 PerCP/Cyanine5.5 (1:100 ...
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bioRxiv - Immunology 2022Quote: ... Followed by ACE2-biotin at 1 μg/mL (RnD Systems) incubation for 30 minutes and then SA-APC (Biolegend) incubation for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... Several positive controls were utilised: (1) polyclonal T-cell simulation with soluble αCD3 (0.5 μg/ml; clone OKT3; Biolegend) and α-CD28 (1μg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... RRID:AB_2315387) and axonal marker anti-neurofilament antibody (1:1000, Clone: SMI-312, # 837904, BioLegend, San Diego, CA, USA; RRID:AB_2566782) at 4°C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were stained at the cell surface with FITC- conjugated anti-CD4 (1:100, BioLegend Cat# 203406 (also 203405), RRID:AB_1227553 ...
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bioRxiv - Immunology 2024Quote: ... cells were harvested and stained for 30min at 4°C with anti-CD25 APC (Biolegend, Cat: 102012, 1:50), and ZombieUV (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked overnight in Odyssey PBS Blocking Buffer (Li-Cor) and stained for 2 hours at RT with Syn1 (1:1,000; Biolegend) or γH2AX (1:1,000 ...
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bioRxiv - Microbiology 2024Quote: ... The sections were rinsed with PBS and incubated with the anti-Neu5Gc (1:500 dilution; Biolegend, San Diego, CA) overnight at 4°C ...
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bioRxiv - Physiology 2023Quote: ... Cell suspensions were incubated with the following antibodies for (20min, on ice): anti-ICAM1-PB (1:25, BioLegend, 322715); anti-VCAM1-PE (1:25 ...
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bioRxiv - Developmental Biology 2024Quote: ... The cells were incubated for 20 min with the following antibodies: Ter119-FITC (BioLegend, Catalog No. 116205, 1/800), Cd45R-FITC (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... then after 2 washes cells were incubated with streptavidin (SA) conjugated to Brilliant Violet 650 (BV650, 1:200, BioLegend) in PBS on ice for 15 minutes ...
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bioRxiv - Immunology 2024Quote: ... CD33 APC (clone P67.6, catalog #: 366605), LOX-1 PE (clone: 15C4, catalog #: 358603), CD11b APC-Cy7 (clone ICRF44, catalog #: 301341) from BioLegend; CD16 BV421 (clone 3G8 ...
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bioRxiv - Immunology 2024Quote: ... Stained spleen or LN samples were fixed with 1-4% phosphate-buffered paraformaldehyde solution (FluoroFix or Fixation Buffer, Biolegend). To detect transcription factors and certain intracellular proteins ...
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bioRxiv - Developmental Biology 2023Quote: ... The cell suspensions were stained with anti-SM-C/2.6 antibody (Fukada et al., 2004) as well as anti-CD45-PE (diluted 1/500, Biolegend), anti-CD31-PE (diluted 1/500 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed three times with Cell Staining Buffer and stained with Alexa Fluor 647 anti-human Ki-67 antibody (1:100) and DAPI (0.5 µg/ml) respectively in 1x Intracellular Staining Permeabilization Wash Buffer (BioLegend). The cells were resuspended in cell staining buffer (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed with FACS-wash (FW-1% FBS in PBS) in 96-well V-shape plates and stained with DAPI (0.1 µg/ml) (Biolegend) for 5 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes were washed three times with TBS-T (TBS with 0.1% Tween-20) and bound Anxs were detected by immunoblotting using Rat anti-FLAG antibody (1:5000; Biolegend) and ECL (ThermoFisher).
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bioRxiv - Microbiology 2023Quote: ... Cells were washed once with cold HMI-9 and resuspended in 200μl cold HMI-9 with rat anti-mouse IgM-FITC (1:500, Biolegend). After one wash with cold HMI-9 ...