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Citations for BioLegend :
8201 - 8250 of 8901 citations for DNAM 1 Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... 500 ng/ml rAspn and 1 μg/ml of ultra-LEAF™ CD44 function blocking antibody (Clone IM7; Biolegend) or equal amount of isotype control IgG2b kappa (Clone RTK4533 ...
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bioRxiv - Immunology 2020Quote: ... washed with PBS and stained for ASC using a primary antibody against the CARD domain (Biolegend; 1:1000 dilution) followed by an Alexa Fluor conjugated secondary antibody (Life technologies ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies included in this study were: mouse IgG2a-anti-β-III-tubulin (Tuj1, 1:400, catalog #801202, Biolegend), chicken-anti-NF200 (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated overnight at 4°C with anti-Aβ primary antibody (6E10 Biolegend, SIG-39320, 1:1000) following incubation with goat anti-mouse secondary antibody (Thermo Scientific ...
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bioRxiv - Genomics 2019Quote: ... The resuspended cells were incubated for 20 min at 4 °C with the fluorophore-labeled antibodies CD45-PerCPCy5.5 (1:50) (Biolegend), Ly51-PE (1:800 ...
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bioRxiv - Immunology 2021Quote: ... and stained with 1 ug/test cell-hashing antibody (TotalSeq C0251, C0254, C0256, C0260, clones LNH-95, 2M2, BioLegend) for 30 minutes at 4C ...
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bioRxiv - Systems Biology 2019Quote: ... and with biotinylated antibodies against a panel of bone marrow lineage markers (CD19, CD11b, CD11c, NK.1.1, Ter119, CD3ε, Gr-1 and B220 (BioLegend)) ...
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bioRxiv - Bioengineering 2019Quote: ... the cell morphological change was examined under bright field microscope and macrophage markers CD36 (2.5 µg per 1×106 cells in 100 µL; Cat. no. 108418, BioLegend) and CD71 (2.5 µg per 1×106 cells in 100 µL ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were incubated overnight at 4°C with mouse anti-HA primary antibody (1:1000, Anti-HA.11, Biolegend), washed 3× in PBS and incubated with Alexa-conjugated anti-mouse secondary antibody (1:500 ...
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bioRxiv - Systems Biology 2019Quote: ... and with biotinylated antibodies against a panel of lineage markers (CD19, CD11b, CD11c, NK.1.1, Ter119, CD3ε, Gr-1 and B220 (BioLegend)) ...
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bioRxiv - Cancer Biology 2019Quote: ... Purified cells were plated at 1×105 cells per well on a 96 well plate coated with 10μg/ml αCD3 (Biolegend), and cultured with 2μg/ml soluble αCD28 (Biolegend) ...
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bioRxiv - Microbiology 2019Quote: ... membranes were reprobed after stripping of previously used antibodies using a pH 2.2 glycine-HCl/SDS buffer with anti-E.coli RNA Polymerase α (1:2,000; BioLegend 663102).
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bioRxiv - Immunology 2019Quote: ... in the presence of 1 μl BV421 anti-CD107a mAb for 2 hr at 37°C (H4A3, Biolegend 328626). Streptavidin M280 Dynabeads were conjugated to biotinylated HLA-C*05:01 refolded with P2-AV or P2-AW as described below.
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bioRxiv - Immunology 2021Quote: ... The cells were washed with PBS/BSA/EDTA and stained either with anti-IgM PE (dilution 1:100, Biolegend) and anti-IgG Alexa FluorTM 647 (dilution 1:600 ...
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bioRxiv - Systems Biology 2021Quote: ... After spinning them down the cells were incubated overnight at 4C with PerCP/Cy5.5 anti-BrdU (1:30 dilution) (BioLegend) in a buffer containing 0.5% Tween 20 / 1% BSA/PBS and RNase (0.8 mg/ml) ...
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bioRxiv - Immunology 2021Quote: ... the cells were fixed for 1 h at RT using the True-Nuclear transcription Factor Buffer Set (BioLegend, 424401). Following the fixation ...
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bioRxiv - Bioengineering 2020Quote: ... Levels of TNF-α mRNA and protein were detected by qPCR (Table 1) and ELISA (BioLegend, San Diego, CA), respectively.
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bioRxiv - Neuroscience 2021Quote: ... for 15 min and cells were stained with fluorophore-conjugated antibodies: CD11b BV605 (1:200, clone M1/70, Biolegend), CD45 PerCP-Cy5.5 (1:200 ...
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bioRxiv - Cancer Biology 2020Quote: Single cell suspension obtained by trypsin treatment was washed and incubated with an anti-mouse CD326 (EpCAM) AlexaFluor 647 antibody (1:2000, clone G8.8, Biolegend). DAPI (10 μg/mL ...
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bioRxiv - Cell Biology 2019Quote: ... Cell surface integrins were labelled with fluorochrome-conjugated antibodies [Alexa Fluor 488 anti-integrin β1 (Clone: HMβ1-1, Biolegend), APC anti-integrin α1 (Clone ...
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bioRxiv - Biochemistry 2021Quote: The following antibodies were used in this study: Anti-ZBP1 clone RonoB19 (WB 1:500, ref. 651602, Rat, BioLegend), anti-TIAR (C-18 ...
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bioRxiv - Biochemistry 2020Quote: ... Tetramers were prepared by mixing 1 or 5 pmol of biotinylated protein monomer with 0.25 to 1.25 pmol of streptavidin-PE (Biolegend #405245) respectively and incubating for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated in 5% dried non-fat milk powder dissolved in TBS-Tween 0,1% (TBST) for 1 h at room temperature (RT) and subsequently incubated with primary anti-HA antibody (Biolegend) followed by incubation by secondary goat HRP-conjugated anti-mouse IgG (Promega) ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated with Calbryte 630TM (2μM; Stratech) for 30 minutes at 37°C followed by staining with CD42b-BV421 (1:100; Biolegend) for 15 minutes at room temperature and supplementation with 2mM CaCl2 ...
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bioRxiv - Microbiology 2021Quote: ... cells were incubated with 100 μl/well of the fixable dead cell viability dye Zombie Aqua (1:800, Biolegend) diluted in PBS for 30 min on ice ...
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bioRxiv - Cell Biology 2021Quote: 1×106 (for immunoblot) or 1.8×107 (for immunoprecipitation) Jurkat Cells were incubated with anti-CD3 (1 μg/ml, OKT3, 317325, BioLegend) and anti-CD28 (1 μg/ml ...
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bioRxiv - Genomics 2020Quote: ... Cells were then stained with a panel of 9 further DNA-oligonucleotide conjugated antibodies (0.33 μg/ml anti-CD71: BioLegend 113802 conjugated with ADT9 [CGAAGAAGGAGT], 1 μg/ml anti-CD41: BioLegend 133919 conjugated with ADT3 [TGTCCGGCAATA] ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-1 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40634-V27H-B) conjugated with PE-streptavidin (BioLegend, 405204), SARS-CoV-2 biotinylated RBD protein (His & AVI Tag ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... primary antibody 4G8 was incubated overnight at a 1:1000 dilution at room temperature (RT) (Biolegend, San Diego, CA). HRP-linked secondary goat anti-mouse antibody at a 1:1000 dilution (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... The cell suspension was stained using allophycocyanin (APC)/Cy7-conjugated rat anti-mouse CD45 antibody (1:200, BioLegend, #101319), PE-conjugated rat anti-mouse EpCam antibody (1:500 ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: Both FLT3L and GM-CSF BMDCs were stained with 1:1000 Zombie Green Fixable Viability Kit (BioLegend, Cat# 423111) and were blocked with anti-mouse CD16/32 (BioLegend ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated at 4°C overnight with the following primary antibodies: mouse anti-C3 (1:200 dilution, 846302, Biolegend), rabbit anti-CD31 (1:200 dilution ...
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bioRxiv - Microbiology 2022Quote: The HA epitope was detected with mouse monoclonal antibody (mAb) HA.11 (diluted 1:1000) (BioLegend, item no. 901515) or rabbit polyclonal antibody (pAb ...
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bioRxiv - Immunology 2022Quote: ... The plate was washed five times after 1-hour secondary antibody incubation and developed with tetramethylbenzidine substrate (Biolegend, 421101). The reaction was stopped with 1 M phosphoric acid after 20 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were stained 20 min with APC-conjugated anti-CD24 antibody (1:100 dilution, 138506, Biolegend, San Diego, CA) and FITC-conjugated anti-Epcam antibody (1:500 dilution ...
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bioRxiv - Immunology 2021Quote: ... counted and up to 1×108 cells were labeled with 1.5-2 μM Carboxyfluorescein succinimidyl ester (CFSE; BioLegend, USA) in 1 mL PBS 1X for 20 min according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... OVA-specific IgG1 was detected using biotinylated anti-mouse IgG1 monoclonal antibody (0.02µg/mL RMG1-1, Biolegend – CA, USA) followed by SA-HRP (1:2000 dilution #P030701-2 ...
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bioRxiv - Neuroscience 2022Quote: ... Cochlea sections were also immunolabeled with rabbit anti-β3 tubulin (1:1000; clone TUJ1, Biolegend, San Diego, CA, USA) overnight at 4 °C followed by goat anti-rabbit Alexa-488 (1:200 ...
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bioRxiv - Genomics 2022Quote: CRISPR/Cas9 RNP-treated Th2 cells were stained intracellularly with APC anti-GATA3 antibody (Biolegend #653806, 1:40 dilution) at room temperature for 30 minutes using True-Nuclear Transcription Factor Buffer Set (Biolegend #424401 ...
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bioRxiv - Immunology 2022Quote: Splenocytes from TregΔCd226 and TregWT mice were plated in a 96-well plate at a concentration of 1,000,000 cells/mL with plate-bound α-CD3ε (2 μg/mL) and plate-bound CD155 (1 μg/mL; BioLegend) for 15 or 30 minutes at 37ºC ...
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bioRxiv - Cancer Biology 2022Quote: ... and lineage-negative cells (Lin-) were analyzed for c-Kit (clone 2B8; eBioscience) and Sca-1 (clone D7; BioLegend) expression to discriminate Kit+ Sca-1- (KL ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated 1:100 with antibodies directed against cell surface proteins CD11b-BV421 (BioLegend, 101251, clone M1/70), CD45-Percp-Cy5.5 (BioLegend ...
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bioRxiv - Cell Biology 2022Quote: ... Syt5/9 (SYSY, cat#105053), Tomosyn-1 (SYSY, cat#183103), Vamp2 (SYSY, Cat#104008) and Snap25 (Biolegend, Cat#836304) were used in Western blotting ...
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bioRxiv - Developmental Biology 2022Quote: ... the cell suspension was pelleted by centrifugation and cells were resuspended in FACS buffer containing 7AAD (BioLegend, 1:100). The flow cytometry analysis was performed on an LSR-II flow cytometer (BD Biosciences) ...
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bioRxiv - Bioengineering 2019Quote: ... PBMCs were stained with CFSE dye at a concentration of 1 μM in PBS for 15 min (Biolegend, 423801) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... Incorporation of BrdU in proliferating intestinal epithelial cells was detected using an anti-BrdU antibody (Biolegend, 1:100 dilution) after deparaffinization of the tissues ...
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bioRxiv - Cancer Biology 2019Quote: ... Experimental values are expressed as a ratio of protein levels over control with the TFIIB (Biolegend, 1:2000 dilution) and Histone H3 (Abcam ...
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bioRxiv - Immunology 2020Quote: ... Oxidative stress was induced using 0.03% H2O2 for 1 hour then cells were washed stained with viability dye Zombie Aqua (Biolegend) and placed on ice before immediate flow cytometry analysis.
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bioRxiv - Biochemistry 2020Quote: ... HA-Flag-tagged and GFP-tagged proteins were detected using HRP-conjugated monoclonal anti-HA (1:5000, BioLegend #901501) or polyclonal anti-GFP antibodies (1:2000 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and a further 1 h incubation with anti-His HRP conjugate (BioLegend; San Diego, CA, USA; cat. no. 652504) diluted (1:2500 ...