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Citations for BioLegend :
8151 - 8200 of 8901 citations for DNAM 1 Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... cells were labeled with an antibody cocktail containing Anti-CD14-PE/Cy7 (Clone 63D3, 367111, BioLegend, 1:100), Anti-CD80-APC (Clone 2D10 ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were washed with PBS containing 1% FBS and incubated with APC anti-His Tag Antibody (BioLegend) for 20 minutes on ice ...
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bioRxiv - Bioengineering 2023Quote: ... The following antibodies were mixed and added directly to the cell suspension: BV421 CD86 (1:200, Biolegend, 105031), PacBlue Ly6G (1:150 ...
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bioRxiv - Immunology 2023Quote: ... cells were washed with FACS buffer and stained with goat-anti-mouse IgG-Alexa Fluor647 (1:400, Biolegend) in FACS buffer for 20 minutes at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Surface markers were subsequently stained on ice for 20 minutes: anti-mouse CD3-PE (1:200, 17A2, Biolegend), anti-mouse CD4-APC (1:200 ...
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bioRxiv - Genetics 2023Quote: ... proteins were transferred onto nitrocellulose membranes and probed with a 1:2,000 dilution of anti-RpoS antibody (Biolegend). For secondary antibody ...
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bioRxiv - Biochemistry 2023Quote: ... the membrane was blocked with 0.7% Na-caseinate pH 7.4-7.6 and 1% BSA and probed with the following primary antibodies: O-GlcNAc (clone RL2, BioLegend), OGT (ab96718 ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% BSA and 0.07% NaN3 in PBS) with additional Zombie Aqua™ (1:1,000 Fixable Viability Kit, BioLegend) to stain for vital cells ...
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bioRxiv - Developmental Biology 2023Quote: ... then for 1 hour at room temperature (RT) with biotinylated secondary antibodies and finally with fluorochrome-labeled (BioLegend) or peroxidase-labeled streptavidin (Beckman Coulter) ...
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bioRxiv - Immunology 2023Quote: ... stained with cell surface antibodies at 1:1200 (anti-CD8: PerCP-Cyanine5.5, eBioscience, # 45-0081-82, or Biolegend, #100734 ...
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bioRxiv - Cell Biology 2023Quote: ... neurons were incubated live with 10 µg/ml of β1-integrin antibody clone HM β1-1 (102202, Biolegend) for 15 min at 37 °C ...
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bioRxiv - Bioengineering 2022Quote: ... 25 μL of fluorescent counting beads (1 million/mL, 424902, Precision Count Beads, BioLegend, San Diego, California USA) were mixed with 100 μL of cell suspension for each condition ...
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bioRxiv - Neuroscience 2023Quote: ... and SMI-32 (using mouse Purified anti-Neurofilament H nonphosphorylated antibodies, 1:12000 dilution; Biolegend, San Diego, CA) immunohistochemistry (note that only sections adjacent to every 3rd Nissl-stained sections were processed for calbindin or SMI-32 ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 million cells were incubated for 20 min at room temperature with Zombie viability dye (Biolegend ref 77143). Then cells were incubated in PBS + 10% FBS to block Fc receptors ...
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bioRxiv - Immunology 2022Quote: ... The c-kit+ fraction was stained with antibodies against CD117 (c-kit APC, clone 2B8, Biolegend, 1/100), Sca-1 (Pacific Blue ...
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bioRxiv - Microbiology 2023Quote: ... gonorrhoeae (1 x 108 CFU) was incubated with 1.5 µl Tag-IT® Violet (TIV) (Biolegend, Catalog # 425101) in 100 µl 1X PBS + 5 mM MgSO4 ...
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bioRxiv - Immunology 2024Quote: ... sections were stained for 3h at room temperature with anti-CD3 PE-Dazzle594 (Biolegend, Cat: 100245, 1:50), anti-CD4 PE (Biolegend ...
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bioRxiv - Physiology 2024Quote: ... followed by 30-min incubation with the following diluted antibodies: BV605-anti-mouse CD45 (BioLegend #103139, 1:200), BV421-anti-mouse Ly6G (BioLegend #127627 ...
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were pre-stained with Fc blocker for 10 min in ice followed by staining at 1:300 with anti-CD64 (139304, PE anti-mouse CD64, clone:X54-5/7.1, BioLegend) and anti-Pdgfra (25-1401-82 ...
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bioRxiv - Immunology 2023Quote: ... anti-mouse CD279 (PD-1) (clone: RMP1-30, PE, Biolegend, cat# 109104; clone: RMP1-30, PE-Cy7, Biolegend, cat# 109110 ...
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bioRxiv - Biochemistry 2023Quote: ... using the following reagents: anti-HA (HA.11 Clone 16B12 Monoclonal antibody, BioLegend 901514, used at 1:1,000), Alexa Fluor 680 donkey anti-rabbit IgG(H+L ...
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bioRxiv - Bioengineering 2023Quote: ... were prepared in 1× Annexin V Binding Buffer (100 mM HEPES, 25 mM CaCl2, 1.4 M NaCl; BioLegend). To analyze cells ...
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bioRxiv - Immunology 2023Quote: ... kidneys were washed three times for 1 hr at room temperature in Ce3D™ Wash Buffer (427710, Biolegend), then cut in half and transferred to Ce3D™ Permeabilization/Blocking Buffer (48 hr ...
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bioRxiv - Cancer Biology 2023Quote: ... A far-red BST2 antibody was used to label BST2-expressing cells (diluted 1:40, BioLegend, cat. 348416). Cell viability was determined by Sytox Blue (diluted 1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... The grids were incubated with mouse anti-HA.11 epitope tag antibody (BioLegend, 901501, 1:1000, RRID: AB_2565006) for 30 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Viability staining was performed using Zombie NIR for 10 minutes at room temperature (1:500 in PBS, BioLegend). Cells were pelleted and resuspended in anti-CD16/32 antibody (1:200 ...
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bioRxiv - Immunology 2023Quote: ... approximately 1.0×106 PBMCs were firstly incubated with LIVE/Dead Zombie Aqua Dye (77143, BioLegend; 1 μL/well) for 15min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed with PBS prior to staining with Zombie NIR Fixable live/dead dye (1:500) (Biolegend) for 20 min at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... The level of CTLA4 was measure by intracellular staining using anti-CTLA4 (Biolegend, Cat#106311, 1:1000 dilution) and the Transcription Factor Staining Buffer Set (eBioscience ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 100 μL Zombie NIR (1:1000 in PBS, Zombie NIR™ Fixable Viability Kit, Biolegend, #423106), transferred into a 96-well plate and incubated for 20 min at 4 °C in the dark ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies included a combination of the following: rat anti-CD4 antibody (Biolegend, UK, cat# 100505, 1:500), guinea pig anti-IBA1 antibody (Synaptic Systems ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were incubated for 30min with the following antibodies in 1:100 concentration: APC anti-mouse CD45 (Biolegend), APC anti-mouse Ter119 (Biolegend) ...
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bioRxiv - Immunology 2024Quote: ... Activated total PBMCs or isolated activated CD4+ and CD8+ T cells were treated with anti-PD-1 (BioLegend, Ultra LEAF purified human anti-PD-1 antibody ...
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bioRxiv - Neuroscience 2024Quote: ... Brain and spleen cells were stained with 1 µL from each FITC anti-mouse CD45 (Biolegend, Cat. 157214), PE anti-mouse CD11b (Biolegend ...
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bioRxiv - Neuroscience 2024Quote: ... and SMI-32 (using mouse Purified anti-Neurofilament H nonphosphorylated antibodies, 1:12000 dilution; Biolegend, San Diego, CA) immunohistochemistry.
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bioRxiv - Microbiology 2024Quote: ... for 10 min at RT and incubated with anti-mCherry developed in mouse (diluted 1:1000, Biolegend, 677702) and anti-TRAP (diluted 1:200 ...
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bioRxiv - Microbiology 2024Quote: ... Primary antibodies against the α-subunit of RNA polymerase were used at 1:5,000 (BioLegend, Lot No. B376827) with fluorophore conjugated antibodies against mice (Li-Cor ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissue for sequencing was incubated for 1 hour with 0.5 μM Sytox™ Blue solution (Biolegend, catalog no. 425305) to counterstain cell nuclei ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.02% Tween-20 in PBS) and incubated with 1 µg of a unique TotalSeq-A nuclear hashing antibody (Biolegend) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... Fixed and blocked hMPCs were incubated overnight at 4°C with an anti-BrdU antibody (1:400 dilution, Biolegend) followed by PBS washes and a 60 min incubation with an Alexa-Fluor 488 conjugated anti-mouse secondary antibody (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... containing 1% BSA and incubated with rabbit monoclonal CTR1 antibody (ab129067) or an isotype control antibody (Biolegend, clone Poly29108) for 1 hour on ice ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained for 20-30 minutes on ice with 1:200 anti-CD11b-BV711 (Clone M1/70; Biolegend) prior to analysis by flow cytometry ...
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bioRxiv - Immunology 2021Quote: ... in PBS with 1% BSA for 10 minutes at 4C and then surface stained with CD45-FITC (HI30, Biolegend) for 30 minutes in the dark ...
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bioRxiv - Developmental Biology 2021Quote: ... E14 cells were collected from culture dishes and incubated for 30 min at 4 °C with CITE-seq cell hashing62 antibody Ab_CD15 (1:200) (Biolegend). XEN-eGFP were collected from culture plates and incubated for 30 min at 4 °C with CITE-seq cell hashing antibody Ab_CD140 (1:200 ...
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bioRxiv - Developmental Biology 2021Quote: ... XEN-eGFP were collected from culture plates and incubated for 30 min at 4 °C with CITE-seq cell hashing antibody Ab_CD140 (1:200) (Biolegend). In the gastruloid sample ...
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bioRxiv - Immunology 2021Quote: ... then stained with the I-A/E-APC antibody (MHCII) (1:1,200) (eBioscience) and positively selected for I-A/E-positive cells using anti-APC Microbeads (Biolegend). The obtained final cell fraction consisted of total mTECs (CD45-EpCAM+Ly51-I-A/E+).
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bioRxiv - Immunology 2021Quote: ... The depleted cell fraction was then stained for 20 min at 4 °C with the CD45-PerCPCy5.5 antibody (1:50) (Biolegend) and the EpCAM-FITC antibody (G8.8 clone ...
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bioRxiv - Immunology 2021Quote: ... The depleted cell fraction was then stained for 20 min at 4 °C with a cocktail of fluorophore-labeled antibodies CD45-PerCPCy5.5 (1:50) (Biolegend), Ly51-PE (1:800 ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were incubated for 1 h on ice in PE/Cy7 anti-mouse CD45.2 antibody (clone:104, BioLegend) and PE-conjugated anti-mouse IL1a antibody (clone:ALF161 ...
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bioRxiv - Molecular Biology 2021Quote: CH12 cells were stimulated to undergo CSR to IgA by treatment with 1-5 μg/mL α CD40 (BioLegend), 5 ng/ml TGF (R&D Systems ...