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Citations for BioLegend :
8101 - 8150 of 8901 citations for DNAM 1 Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... CD64 (clone X54-5/7.1, PE-Cy7), IgG1 (clone RMG1-1, APC), IgG2a (clone RMG2a-62, PerCP-Cy5.5) were purchased from BioLegend, CA ...
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bioRxiv - Immunology 2020Quote: ... #SK-4105) and others were also incubated with an AF647 anti-neutrophil antibody (1:50, Biolegend UK #127609). Slides were scanned on a ZEISS Axio Scan.Z1 slide scanner.
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bioRxiv - Cell Biology 2019Quote: Panel 3 (fresh in vivo tissue): anti-CD45-Brilliant Violet 570 (HI30 clone, 304034, BioLegend, dilution 1:50), anti-CD235a-Pacific Blue (HI264 clone ...
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bioRxiv - Immunology 2020Quote: ... and Nlrp3-/- BMDMs were stained with anti-mouse MHC 1 Kb-PE labeled antibodies (BioLegend, clone AF6-88.5) and they were positive by FACS analysis.
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bioRxiv - Biochemistry 2019Quote: ... 1 μL of antibody (α-Flag-M2 antibody, Sigma-Aldrich / α-HA, Biolegend/ α-TAP, Thermo Fisher Scientfic) was added to the lysate and rotated for 3 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-Phospho-c-Jun (Ser73) (D47G9) (Cell Signalling, #3270) and Ly-6A/E (Sca-1) (Biolegend, cat#108101). Appropriate Alexa Fluor labelled secondary antibodies (ThermoFischer Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... 50 μl of blood was resuspended in 1 ml of red blood cell (RBC) lysis buffer (Biolegend™) for 10 min on ice followed by two washes with 1 ml phosphate buffer saline (PBS) ...
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bioRxiv - Immunology 2021Quote: ... 108 yeast were pelleted and resuspended in 1 mL PBE (PBS with 0.5% BSA and 0.5 mM EDTA) containing 1:100 anti-FLAG PE antibody (BioLegend). Yeast were stained at 4° for 75 minutes ...
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bioRxiv - Immunology 2021Quote: Mice were injected intravenously under isoflurane anesthesia once daily with 1×105 U recombinant mouse IFNγ (575306, Biolegend) in 150 μL PBS ...
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bioRxiv - Neuroscience 2019Quote: ... anti-CD45-FITC or anti-CD45-APC/Cy7 (1:100, BD Pharmingen Clone 30-F11 553080; Biolegend, 103116), and anti-Cd11b-BV421 (1:100 ...
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bioRxiv - Developmental Biology 2021Quote: The following antibodies and dilutions were used: rabbit anti-transformation related protein 63 (P63, 1:250, 619002, Biolegend), rabbit anti-red fluorescent protein (tdT ...
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bioRxiv - Cell Biology 2020Quote: ... overnight at 4 °C and followed by incubation with Streptavidin conjugated to Alexa 488 (1:200, Biolegend, #405235) and an anti-mouse secondary conjugated (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... and CV05) were stained with a divergent set of antibodies: CD19-PE 1:50 (clone HIB19, BioLegend, 302207), CD38-PEcy7 1:50 (clone HIT2 ...
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bioRxiv - Immunology 2020Quote: CH12 cells were stimulated to undergo CSR to IgA by treatment with 1-5 μg/mL αCD40 (BioLegend), 5 ng/ml TGFβ (R&D Systems ...
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bioRxiv - Immunology 2021Quote: ... the isolated B cells were incubated with 1 μl Zombie UV Fixable Viability kit (Biolegend, cat. no. 423107) per 1 ml cell suspension for 30 min on ice ...
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bioRxiv - Immunology 2022Quote: ... intracellular staining in Saponin Buffer (0.1% Saponin, 1% BSA in PBS) was done using anti-IFNγ (clone XMG1.2, Biolegend), anti-TNF (clone ...
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bioRxiv - Neuroscience 2022Quote: ... before washing and transferring to PBS and treating with Zombie NIR (BioLegend, 1:100, 15 min at RT) to label dead cells ...
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bioRxiv - Neuroscience 2022Quote: ... and 3 washes were performed prior to secondary antibody staining with anti-rabbit bv421 1:500 (BioLegend 406410) with 3 additional washes after secondary antibody ...
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bioRxiv - Immunology 2022Quote: Frozen thymus tissue sections (7μm) were fixed in acetone and stained using antibodies specific for CD69 (1:100, H1.2F3, BioLegend), Ly51 (1:200 ...
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bioRxiv - Bioengineering 2022Quote: ... and then 1:200 dilution of Alexa Fluor 488 mouse anti-rat CD45 (BioLegend, San Diego, CA, USA), PE mouse anti-rat CD31 (BD biosciences ...
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bioRxiv - Immunology 2022Quote: ... were injected into the microfluidic circulation in HBSS with 1% FBS supplemented with 5 nM CXCL10 (Biolegend 573604) or vehicle (PBS) ...
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bioRxiv - Genetics 2022Quote: ... PT3 bone marrow-derived CD34+ cells were stained using 1:40 APC-conjugated CD34 (clone 581, 343509, BioLegend). For mtscATAC-seq and ASAP-seq experiments residual granulocytes were excluded by staining of cells using 1:50 PE-conjugated CD66b (clone G10F5 ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated O/N in 50% block solution with anti-Prox1 1:500 (Biolegend, San Diego, CA; Rb), anti-ChAT 1:1K (Chemicon ...
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bioRxiv - Immunology 2024Quote: ... all cells were blocked with 1:50 dilution of TruStain FcX anti-mouse CD16/CD32 (Clone 93, Biolegend) and Fixable Viability Dye (eBioscience ...
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bioRxiv - Molecular Biology 2022Quote: ... clone 500A2 (all at 1:100; produced in-house) and with the Zombie Aqua fixable dye (423101, Biolegend).
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bioRxiv - Physiology 2022Quote: ... Organoids were harvested in cold Serum-Free Defined Medium media and incubated with Zombie (BioLegend 423107, 1:100) for 15 minutes in the dark at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... Washed and filtered single-cell suspension were stained with viability dye (Zombie NIR, 1:500, BioLegend, no. 423105), blocked with Fc-block (1:200 ...
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bioRxiv - Cancer Biology 2024Quote: ... For senescence and exhaustion NK cell evaluation the following antibodies were used: anti-PD-1 (clone EH12.2H7, Biolegend), - CD57 (clone NK-1 ...
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bioRxiv - Genomics 2024Quote: ... Approximately 1 million MNCs per tissue site were washed in FACS buffer and resuspended in TrueStain FcX (BioLegend) for 10 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... anti-CD45.1 A20, anti-CD45.2 104, anti-CD8 53-6.7 conjugated to different fluorophores depending on experiment, all Biolegend). Dead cells were excluded by staining cells with a fixable Live/Dead dye (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-PD-1 (Bio X Cell, RMP1-14, BE0146, 10 μg/mL or Biolegend, 114102, 10 μg/mL), anti-CD38 (Novus ...
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bioRxiv - Molecular Biology 2024Quote: ... and stained with a panel of 40 fluorescent reagents (Supplementary Table 1) supplemented with Monocyte Blocker (BioLegend 426102) and Brilliant Buffer Plus (BD Biosciences 566385) ...
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bioRxiv - Neuroscience 2024Quote: Primary antibodies used in this study included mouse IgG2a-anti-β-III-tubulin (Tuj1, 1:400, #801202, Biolegend) to label SGNs ...
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bioRxiv - Neuroscience 2024Quote: ... and SMI-32 (using mouse Purified anti-Neurofilament H nonphosphorylated antibodies, 1:12000 dilution; Biolegend, San Diego, CA) immunohistochemistry.
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bioRxiv - Immunology 2024Quote: ... Cells were then washed and surface stained with CD4-PE-Cy5 (Clone RPA-T4, BioLegend 300510, 1:400), CD69-BUV496 (Clone FN50 ...
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bioRxiv - Developmental Biology 2023Quote: ... Western blot analysis was performed using anti-HA.11 epitope tag antibody (BioLegend, 901501, 1:1000, RRID: AB_2565006), anti-FLAG (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: Cells were first washed with PBS then resuspended in Zombie Aqua Viability Dye (1:500 in PBS, Biolegend). For extracellular staining ...
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bioRxiv - Cell Biology 2023Quote: ... and a 1:500 dilution of Alexa Fluor™ 647 anti-murine Siglec-F (BioLegend, Cat. No. 155519). Depending on the fluorophores selected in each panel ...
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bioRxiv - Immunology 2023Quote: ... cells were re-suspended in PBS and treated with anti-CD16/CD32 (Clone 93, Biolegend, 101301, 1:200) for 15 min on ice ...
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bioRxiv - Immunology 2023Quote: ... cells were centrifuged and resuspended in 1 ml of −20°C cold TruePhos™ Perm Buffer (BioLegend, USA) and incubated at −20°C for 1 hour ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The cell suspension was stained with a panel of antibodies against CD45-Alexa 700 (1:1200; Biolegend; #147716) and CD11b-PE dazzle (1:800 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Dead cells were excluded using the live/dead stain Zombie NIR fixable viability dye (1:2000; Biolegend; #423105). The samples were run on a Cytek® Northern Lights™ cytometer (Cytek biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... The following antibodies were used to assess post-transplant BM reconstitution: CD45.1 FITC (A20, BioLegend 110706; 1:200), CD45.2 Pacific blue (104 ...
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bioRxiv - Immunology 2023Quote: ... and ST2 were determined by flow cytometric analyses using FITC-labeled anti-mouse FcεRIα (clone MAR-1, BioLegend), APC-labeled anti-mouse CD117 (clone 2B8 ...
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bioRxiv - Immunology 2023Quote: ... was added to the wells for 1 hour at RT prior to developing with 3,3’,5,5’-tetramethylbenzidine (TMB) substrate solution (BioLegend). The reaction was stopped by adding 0.1M sulfuric acid and the optical density was determined at 450 nm using a SpectraMax iD5 spectrophotometer.
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bioRxiv - Immunology 2023Quote: ... samples were incubated in 1:200 dilution of donkey antirabbit IgG antibody with Alexa Fluor 488 conjugation (BioLegend) at RT for 30 min (protected from light) ...
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bioRxiv - Immunology 2023Quote: ... Cells were next resuspended in 50 µl (per mouse) lavage buffer containing 0.5 µg biotinylated anti-mouse FcεRIα (clone MAR-1; Biolegend) and incubated 20 minutes at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Both cell fractions were stained with cell hashing antibodies (1:1000, #2 for heart, #3 for blood, Biolegend) and Propidium iodide (1:100 ...
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bioRxiv - Cell Biology 2022Quote: ... pelleted cells were resuspended in MACS buffer containing anti-CD45-FITC (1:1000, clone 30-F11, Biolegend, 103108), PI (1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... magnetic activated cell sorting (MACS) was applied using the mouse IgM BG-1 antibody (BioLegend, San Diego, CA) coupled to α-mouse IgM microbeads (Miltenyi Biotec ...