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Citations for Roche :
1651 - 1700 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: Genomic DNA (gDNA) was isolated from mouse ear punch biopsies using the PCR Template Preparation kit (Roche). Targets was PCR amplified using Taq Polymerase (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclear pellets were washed once with 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI) (Roche, 10236276001) in methanol (1 μg/ml) ...
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bioRxiv - Molecular Biology 2024Quote: ... Targets was PCR amplified using Taq Polymerase (Roche) and FailSafe™ Buffer J (Epicentre ...
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bioRxiv - Genomics 2024Quote: Kapa HiFi Hotstart ReadyMix (KAPA Biosystems, Inc./Roche ...
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bioRxiv - Genomics 2024Quote: Kapa HiFi Hotstart ReadyMix (KAPA Biosystems, Inc./Roche, Wilmington. MA, USA; cat. no. KK2602)
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bioRxiv - Neuroscience 2024Quote: ... Using the KAPA Library Quantification kit for Illumina platforms (KAPA Biosystems, Wilmington, MA), libraries were quantitated and pooled equimolarly ...
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bioRxiv - Genomics 2024Quote: ... whole blood (14.0 – 17.0 mL per subject) was collected using Cell-Free DNA Collection tubes (Roche Diagnostics; Rotkreuz, Switzerland), with no in-clinic processing (e.g. ...
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bioRxiv - Neuroscience 2024Quote: ... Blood glucose levels were measured using glucometer (Accu-Chek, Aviva, Roche) every week by fasting mice for 2–3h and then withdrawing a drop of blood from the tail vein ...
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bioRxiv - Molecular Biology 2024Quote: ... containing protease inhibitor cocktail (Roche) and phosphatase inhibitors (Roche) ...
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bioRxiv - Microbiology 2024Quote: ... 10 mM NaCl, 1.5 mM MgCl2, 1 mM DTT, 100 μg/ml PMSF, 1 μg/ml pepstatin, and Roche Complete Protease Inhibitor Cocktail) with 100 U/ml of OmniCleave nuclease (Epicentre ...
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bioRxiv - Microbiology 2024Quote: ... 1.5 mM MgCl2, 0.1% Triton X-100, 1 mM DTT, 100 μg/ml PMSF, 1 μg/ml pepstatin, and Roche Complete Protease Inhibitor Cocktail), incubated for 20 minutes on ice ...
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bioRxiv - Microbiology 2024Quote: ... 1.5 mM MgCl2, 0.1% IGEPAL Nonidet P-40, 1 mM DTT, 100 μg/ml PMSF, 1 μg/ml pepstatin, and Roche Complete Protease Inhibitor Cocktail) and transferred to a Dounce homogenizer and homogenized with 20 slow strokes ...
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bioRxiv - Microbiology 2024Quote: ... samples were collected and plasma HIV RNA levels tested by quantitative rRT-PCR (Roche Cobas Ampliprep/Cobas Taqman HIV-1 test ...
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bioRxiv - Microbiology 2024Quote: ... 0.5% w/v sodium deoxycholate) with a 1x protease inhibitor cocktail (Roche, VWR, Cat# PI88266) and 0.5% v/v SUPERase In (Life Technologies ...
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Synthetase and Hydrolase Specificity Collectively Excludes 2’-Deoxyguanosine from Bacterial AlarmonebioRxiv - Microbiology 2024Quote: ... and lactate dehydrogenase (LDH, from rabbit muscle, Roche). Total reactions are as follows:
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bioRxiv - Microbiology 2024Quote: ... genes were amplified using KAPA HiFi HotStart ReadyMix (Roche cat # KK2601), cloned using NEBuilder HiFi DNA Assembly cloning kit (NEB ...
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bioRxiv - Microbiology 2024Quote: ... The engorged individuals were maintained for two days at 25°C and then stored in tissue lysis buffer (Roche, Prague, Czech Republic) at - 20°C in pools of 5 females for subsequent DNA isolation and nested PCR.
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bioRxiv - Microbiology 2024Quote: Total DNA isolated using the High Pure PCR Template Preparation Kit (Roche Diagnostics, Indianapolis, IN) according to the manufacturer’s instructions was used as a template for nested PCR amplification (detection limit 10-2 parasites per sample ...
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bioRxiv - Microbiology 2024Quote: ... cells were washed with cold PBS and lysed using NP-40 lysis buffer (P0013F, Beyotime, China) supplemented with a protease inhibitor cocktail (04693132001, Roche, Switzerland). The resulting cell lysates were each incubated overnight at 4 [with Dynabeads (Sc-2003 ...
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bioRxiv - Microbiology 2024Quote: ... NRP2-Fc point mutations were introduced by PCR mutagenesis using Kapa HiFi DNA polymerase (Kapa Biosystems), according to the QuikChange site-directed mutagenesis manual ...
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bioRxiv - Microbiology 2024Quote: ... protease inhibitor (complete; Roche, Indianapolis, IN, USA)) and analyzed by western blot.
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bioRxiv - Microbiology 2024Quote: ... we isolated RNA from M-SIV and T-SIV using the High Pure Viral RNA Kit (Roche), converted it to cDNA with the Cloned AMV First-Strand cDNA Synthesis Kit (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... the master mix was added and the plate was placed in a LightCycler 480 II (Roche). The results were evaluated by Delta delta CT method considering the Cp values obtained from LightCycler 480 Software release 1.5.0 ...
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bioRxiv - Microbiology 2024Quote: ... the manufacturer’s instructions were followed (New England Biolabs and Roche). Separated DNA fragments were recovered from agarose using the Qiagen gel extraction kit ...
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bioRxiv - Microbiology 2024Quote: ... with their respective dilution in 5% skimmed milk in PBS-tween 0.1% were used: anti-HA-HRP (3F10) (N°12013819001, Roche) 1/10,000 ...
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bioRxiv - Microbiology 2024Quote: ... BSA in TBST (TBSTB) and probed with rat monoclonal Anti-HAHA High Affinity antibody (clone 3F10, cat. no. 11867423001, Roche) diluted 1:1000 in TBSTB for 1 hour at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... were stained with rat monoclonal Anti-HAHA High Affinity antibody (clone 3F10, cat. no. 11867423001, Roche). Alternatively ...
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bioRxiv - Molecular Biology 2024Quote: ... whole cell lysate samples were prepared by resuspension and trituration of cell pellets in a lysis buffer (20 µM Tris-HCl pH 7.4, 1 µg/mL DNaseI, 1X cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche)) ...
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bioRxiv - Microbiology 2024Quote: ... prepared for NGS by shotgun library preparation (KAPA HyperPrep Kits, Roche), and then sequenced on either MiSeq (MiSeq 500-cycle sequencing kit version 2 ...
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bioRxiv - Microbiology 2024Quote: The rapid translation system has been performed according to the producer’s protocol with slight modifications (Roche, Rapid Translation System RTS 100 ...
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bioRxiv - Microbiology 2024Quote: ... 5 mM DTT) supplemented with EDTA-free protease inhibitor cocktail (Roche) and lysed by sonication for ten cycles of 30 pulses of sonication and 30 s breaks using a Hielscher UP200st set to pulse=70% ...
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bioRxiv - Microbiology 2024Quote: ... A two – step PCR protocol was used to prepare the sequencing library: a first amplification (target PCR) was carried out with 1X KAPA HiFi HotStart DNA polymerase (Roche), 0.3 µM of each 16S primer and 2 µL of template DNA ...
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bioRxiv - Immunology 2024Quote: ... and complemented with protease (#4693124001) and phosphatase (#04906837001) inhibitors from Roche (Basel, Switzerland) during 20 minutes ...
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bioRxiv - Immunology 2024Quote: ... complexed to 30µg dotap (Roche/Merck) respectively ...
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bioRxiv - Immunology 2024Quote: ... complexed to 30µg dotap (Roche/Merk) in HBSS (Gibco/Thermoscientific ...
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bioRxiv - Immunology 2024Quote: ... Real-time PCR analysis was performed on LightCycler 480 II (Roche, Pleasanton, USA) equipped with SYBR green detector (TaKaRa Bio ...
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bioRxiv - Immunology 2024Quote: ... 0.5mg/mL of Collagenase D (Roche, 11088882001), and 50μg/mL of DNase I (Worthington-biochem ...
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bioRxiv - Microbiology 2024Quote: DNA amplification for cloning was made by KAPA HiFi HotStart ReadyMix (Roche) according to manufacturer instruction ...
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bioRxiv - Immunology 2024Quote: ... Resulting material was quantified using the KAPA Library Quantification Kit for Illumina platforms (KAPA Biosystems) and sequenced with PE42 sequencing on the NextSeq 500 sequencer (Illumina).
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bioRxiv - Immunology 2024Quote: ... 20 mg/mL DNase I (Roche), and 125 U/mL collagenase D (Roche ...
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bioRxiv - Immunology 2024Quote: ... 1× KAFA HiFi enzyme and buffer (Roche, #KK2502), 0.5 µM Smart-seq3 forward primer and 0.1 µM Smart-seq3 reverse primer ...
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bioRxiv - Immunology 2024Quote: ... unlabeled streptavidin was prepared by incubating 190 µg streptavidin (Roche, Basel, Switzerland) in the presence of a 100-fold molar excess of biotin in 1X Gibco PBS in a final volume of 250 μl ...
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bioRxiv - Immunology 2024Quote: ... kidneys were enzymatically digested with 400 µg/ml collagenase D (Roche) and 10 U/ml DNase I (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... Kidney tissue was enzymatically digested with collagenase D at 0.4 mg/ml (Roche, Mannheim, Germany) and DNase I (10 μg/ml ...
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bioRxiv - Immunology 2024Quote: ... beads and lysis buffer (20 mM Tris pH 7.4, 120 mM NaCl, 1 mM EDTA, 1% Triton-X-100, 0.5% sodium deoxycholate, 1× protease inhibitor cocktail [Roche])) ...
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bioRxiv - Immunology 2024Quote: ... Quantitative PCR (qPCR) was performed using the SYBR Green Master Mix (Roche) and the StepOnePlus Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Immunology 2024Quote: ... Quantitative real-time PCR was performed with LightCycler 480 SYBR Green I Master detection kit (Roche 04707516001) on a Light Cycler 480 machine (Roche) ...
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bioRxiv - Immunology 2024Quote: ... and adding Complete Mini Protease Inhibitor Cocktail Tablet (Roche) and 100μl NP40 (BDH) ...
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bioRxiv - Immunology 2024Quote: ... and IgM repertoires was amplified with a multiplex primer set5 using the FastStart High Fidelity PCR System (Roche) under the following conditions ...
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bioRxiv - Immunology 2024Quote: ... on a Light Cycler 480 machine (Roche). Three technical replicates were performed for each sample ...