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Citations for Roche :
1451 - 1500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Quantitative Western blot analysis was employed to determine concentration of recombinant protein using a recombinant GFP standard (Roche, 11814524001) with a defined concentration of 1 mg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... pH 7.5) supplemented with 1 mM PMSF and protease inhibitor cocktail (Roche). Lysates were then reduced and alkylated with 10 mM DTT and 100 mM of 2-Chloroacetamide (30 min each step at 30°C ...
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bioRxiv - Cell Biology 2024Quote: ... and 1x Pen/Strep (Roche). Lenti-X 293T cells were kindly provided by the lab of Ralf Brandes/Matthias Leisegang ...
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bioRxiv - Cell Biology 2024Quote: ... 6mM Tris HCl pH 6.8) supplemented with EDTA-free protease inhibitor cocktail (Roche). Tissues were disrupted using potter homogenizer (2x 25 strokes at 2000rpm) ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative RT-PCR (qRT-PCR) was performed using FastStart SYBR Green Master kit (Roche, Indianapolis, IN).
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bioRxiv - Cell Biology 2024Quote: ... mRNA levels were determined using gene-specific primers by SYBR green nucleic acid labeling (Selleckchem PCR Mastermix, #B21202) in a Lightcycler 480 (Roche, Indianapolis, IN). Fold change was calculated using the delta delta C(t ...
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bioRxiv - Cell Biology 2024Quote: ... EDTA-free protease inhibitor tablet (Roche)] and rotated for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... EDTA-free protease inhibitor cocktail tablet (Roche)] ...
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bioRxiv - Cell Biology 2024Quote: ... EDTA-free protease inhibitor tablet (Roche)] was added to supernatant to adjust salt concentration to 500 mM KCl final concentration ...
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bioRxiv - Cell Biology 2024Quote: ... and 1x Protease inhibitor cocktail (34044100, Roche Diagnostics GmBH). Cells were lysed by spinning at 70000 x g for 1 hour at 4°C and collecting the supernatant ...
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bioRxiv - Cell Biology 2024Quote: ... plus protease inhibitors (cOmplete, Roche, PMSF, Sigma) on ice ...
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bioRxiv - Developmental Biology 2024Quote: ... qPCR was performed with the Roche LightCycler 96 using FastStart Essential DNA Green Master (Roche; 06402712001). As reference gene we used the housekeeping gene Rplp0 ...
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bioRxiv - Developmental Biology 2024Quote: ... + 5% SDS + 2x cOmplete protease inhibitor cocktail (Roche). After centrifugation at 14k rpm for 10 min at RT ...
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bioRxiv - Cell Biology 2024Quote: ... DNA was stained with DAPI solution (Roche). Antibody details are provided in Table S3.
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bioRxiv - Cell Biology 2024Quote: ... HA (rat; Roche), Sec31 (mouse ...
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bioRxiv - Cell Biology 2024Quote: ... 1x EDTA-free complete protease inhibitor cocktail (Roche)] and lysis was initiated by addition of TritonX-100 to 0.1 % ...
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bioRxiv - Cell Biology 2024Quote: ... 1x complete protease inhibitor cocktail (Roche)] ...
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bioRxiv - Cell Biology 2024Quote: ... or DLD1 cells: anti-HA (Rat, Roche #ROAHAHA, 1:500), anti-SAS-6 (Mouse ...
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bioRxiv - Cell Biology 2024Quote: ... washed twice with PBS containing Complete Mini Protease Inhibitor Mixture (Roche Applied Science), resuspended in Laemmli buffer containing 1 mm DTT ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was quantified by qPCR with FastStart Essential DNA Green Master Mix (Roche, Ltd., Germany) and a real-time PCR instrument LightCycler (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... We used the Agilent Bioanalyzer to assess the quality of the library and a qPCR approach with the KAPA Library Quantification Kit (Roche; P/N: KK4873) on the QuantStudio 12K device to quantify the library ...
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bioRxiv - Cell Biology 2024Quote: Cell lysates from mouse tissues were prepared in RIPA buffer (Beyotime, P0013B) containing Protease Inhibitor Cocktail (Roche, 11873580001) and 1mM PMSF ...
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bioRxiv - Cell Biology 2024Quote: ... and a sgRNA preceded by a rrk1 leader and followed by a Hammerhead Ribozyme as described in [17] was digested and prepared for gap repair as follows: the vector pJB166 was purified from DH5α bacterial cells using the Genopure plasmid midi kit (Roche, 03143414001) and diluted to 1 μg/μL ...
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bioRxiv - Cell Biology 2024Quote: ... media containing 24 μL of X-tremeGENE 9 DNA (Roche) and added to HEK cells ...
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bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used: anti-HA (Rat, Roche #ROAHAHA, 1:500), anti-RFP (Rabbit ...
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bioRxiv - Cell Biology 2024Quote: ... Complete EDTA-free protease inhibitor cocktail tablets were from Roche Diagnostics GmbH (Mannheim ...
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bioRxiv - Cell Biology 2024Quote: ... The chromosome V was visualized with a biotin-labelled probe generated by amplifying the endogenous 5s rDNA locus by PCR and labelling was performed with the Biotin-nick translation mix (Roche) according with manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: ... solution containing 1 μM of Y27362 (Roche), washed and digested for an additional 30 minutes in 0.25% trypsin (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... EDTA-free Protease Inhibitor Cocktail (Roche –11836170001).
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bioRxiv - Cell Biology 2024Quote: ... Cells were scraped into Laemmli buffer containing protease inhibitor cocktail (Roche) and pelleted to remove debris ...
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bioRxiv - Cell Biology 2024Quote: ... and KAPA qPCR (Kapa Biosystems KK4824), cDNA were sequenced on an Illumina HiSeq4000 at UCI’s Genomics Research and Technology Hub to achieve an average of 50,000 reads per cell.
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bioRxiv - Cell Biology 2024Quote: ... 50 µg of cryoground tissue powder was placed in a 1.5mL lobind tube and brought to 1 mL with lysis buffer (1% triton, 1x PBS, 1x Roche Complete protease inhibitor cocktail ...
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bioRxiv - Cell Biology 2024Quote: Apoptosis assay was performed using Annexin V FLUOS staining kit (Roche), and the manufacturer’s instructions were followed with slight modifications ...
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bioRxiv - Cell Biology 2024Quote: ... 1x EDTA-free complete protease inhibitor cocktail (Roche)] ...
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bioRxiv - Cell Biology 2024Quote: ... 1x EDTA-free complete protease cocktail (Roche)] and stored at -20°C.
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bioRxiv - Cell Biology 2024Quote: ... in Buffer D (20 mM HEPES pH 7.9, 150 mM KCl, 20% glycerol, 0.2 mM EDTA, 0.2% Triton X-100 and complete Roche inhibitor) overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... 100 synchronized young adult worms (24h post-L4 stage) were picked into 30 μl of 1XTE (Tris-EDTA Buffer, pH=8) containing 1X complete protease inhibitor cocktail (Roche) and flash-frozen in liquid nitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then digested for 2h in 1mg/ml Collagenase A (Roche, Basel, Switzerland) solution containing 1 μM of Y27362 (Roche) ...
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bioRxiv - Neuroscience 2024Quote: ... 0.2 mg/ml BSA (Roche, 10711454001), 2× SSC ...
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bioRxiv - Plant Biology 2024Quote: ... anti-GFP (Roche), anti-HA (high affinity clone 3F-10 ...
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bioRxiv - Cancer Biology 2024Quote: ... with EDTA-free Protease Inhibitor Cocktail (Roche, Cat# 4693159001) and PhoSTOP (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... The rRNA-depleted RNA libraries were prepared using the KAPA RNA HyperPrep Kit (Roche) and subjected to the Agilent 2100 Bioanalyzer for quality and concentration ...
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bioRxiv - Cancer Biology 2024Quote: ... and DAPI 10236276001 (Roche Diagnostics, Mannheim, Germany). Kinase inhibitors used were Vandetanib (AstraZeneca PLC ...
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bioRxiv - Biophysics 2024Quote: ... EDTA-free Protease Inhibitor Cocktail (Roche), cleared by centrifugation ...
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bioRxiv - Biophysics 2024Quote: ... to a final molal concentration of 25 ng/μL and amplified using primers specific to each oligonucleotide subpool with KAPA SYBR FAST qPCR (KAPA Biosystems; 1-ng template). A second PCR amplification was performed with KAPA HiFi (KAPA Biosystems ...
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bioRxiv - Biophysics 2024Quote: ... 0.1 % SDS) and subsequently resuspended in 900 µL shearing buffer including 1x protease inhibitors Complete Mini cocktail (Roche). Chromatin was sheared by sonication in 15 ml Bioruptor tubes (Diagenode ...
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bioRxiv - Biophysics 2024Quote: ... A second PCR amplification was performed with KAPA HiFi (KAPA Biosystems; 1-μL qPCR template, 15 cycles maximum). We amplified corresponding regions of pSC101_TetR_specR with primers that linearized the backbone ...
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bioRxiv - Microbiology 2024Quote: ... using the LightCycler FastStart DNA Master HybProbe kit (Roche Diagnostics) supplemented with SYBR Green ...
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bioRxiv - Cell Biology 2024Quote: ... using SYBR Green I Master Mix (4887352001, Roche). Specific primers for ChIP-qPCR analysis are listed in Suppl ...
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bioRxiv - Immunology 2024Quote: ... 1 U HiFi enzyme mix (Roche), 1X buffer ...