Labshake search
Citations for Roche :
1451 - 1500 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... 20 ng cDNA was used for real-time qPCR reactions with KAPA SYBR FAST qPCR Kit (Catalog no. KK4618, Roche, Basel, Switzerland) using a CFX Opus Real-Time PCR System (Bio-Rad Laboratories) ...
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bioRxiv - Plant Biology 2024Quote: ... 0.1% Triton X-100, 0.2% Nonidet P-40, 0.6 mM PMSF, and 20 µM MG132 in a Roche protease inhibitor cocktail) was added ...
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bioRxiv - Plant Biology 2024Quote: ... The transformants were screened on MS plants with 1% sucrose and 0.8% agar containing 40mg/L hygromycin (Roche, no. 10843555001). For each construct ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was isolated using TRIzol reagent (Roche, Switzerland) and purified using Direct-zol™ RNA Miniprep Plus kit (Zymo Research ...
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bioRxiv - Microbiology 2024Quote: ... EDTA-free Protease inhibitor cocktail (Roche, USA). Cell lysis was accomplished by using a FisherbrandTM Model 505 Sonic Dismembrator (Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... and 10 mg DNaseI (Roche) and incubated for 30 min on ice ...
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bioRxiv - Microbiology 2024Quote: ... 12.5 mM Imidazole) supplemented with complete EDTA-free protease cocktail inhibitor (Roche), 400 mg lysozyme (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... Protease inhibitors (Roche) were added ...
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bioRxiv - Microbiology 2024Quote: ... pH 8.0 with 1 µL of 100x protease inhibitor cocktail (Roche) during sonication in water bath for 10 min followed by probe sonicated with VibraCell probe (Sonics & Materials ...
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bioRxiv - Genomics 2024Quote: ... protease inhibitor cocktail ml(Roche); and SUPERaseIn RNase Inhibitor (Ambion)] ...
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bioRxiv - Genomics 2024Quote: ... the embryos were transferred to pre-hybridization buffer (50% deionized formamide, 5x SSC pH 4.5, 2% Roche Blocking Reagent ...
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bioRxiv - Genomics 2024Quote: ... pH 9.5) and alkaline phosphatase activity was visualized by incubating in BM purple reagent (Roche) in the dark with gentle agitation ...
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bioRxiv - Genomics 2024Quote: ... and incubated overnight at 4°C with Anti-Dig-AP antibody (Roche, 1:5000) in 1% lamb serum ...
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bioRxiv - Genomics 2024Quote: ... in vitro synthesized from a linearized plasmid using RNA Labeling Mix (Roche) and T3 RNA polymerase (Roche) ...
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bioRxiv - Genomics 2024Quote: ... and T3 RNA polymerase (Roche). Embryos were fixed with 4% paraformaldehyde (PFA) ...
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bioRxiv - Biophysics 2024Quote: ... 1x EDTA-free complete protease inhibitor cocktail (Roche, 5056489001) and phosphatase inhibitor cocktails (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 million cells were pre-extracted on ice with ice-cold 30 µl CSK buffer (25 mM HEPES pH 7.4, 50 mM NaCl, 1 mM EDTA, 3 mM MgCl2, 300 mM sucrose, 0.2% Triton X-100, 1 Roche cOmplete protease inhibitor cocktail tablet per 50 ml of buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... the mutation-containing region was amplified by PCR using the FastStart high-fidelity PCR system (Roche, Nutley, NJ) or the GC-Rich PCR system (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... qPCR was performed on genomic DNA from all 69 tumor sections and the patient’s blood using the LightCycler 480 SYBR Green I master mix and LightCycler 480 qPCR machine according to the manufacturer’s recommendations (Roche, Nutley, NJ). Measurements were triplicated and data were analyzed using the standard curve method ...
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bioRxiv - Cancer Biology 2024Quote: ... 50ng of gDNA was used as template per sample in each reaction and 35 cycles of PCR amplification were performed with KAPA HiFi HotStart Ready Mix (2x, KAPA Biosystems, Wilmington, MA). Multiplexed PCR reactions were purified using a 2X volume ratio of KAPA pure SPRI beads (KAPA Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: ... Full-length RNA was made into libraries using the KAPA stranded mRNA library prep kit (Roche, Nutley, NJ) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The extracted DNA was RNAse treated (40 μg / ml) (Roche, Nutley, NJ) for 1 h at 37°C before being phenol chloroform extracted and ethanol precipitated ...
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bioRxiv - Cell Biology 2024Quote: ... and stranded libraries were prepared using KAPA mRNA HyperPrep Kit (7961901001, Roche) and sequenced using NovaSeq S1 at the UNC Sequencing Core ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA (1 μg) was reverse transcribed to cDNA using the Transcriptor First Strand cDNA Synthesis Kit (catalog no. 04379012001, Roche), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were washed in sterile-filtered PBS (pH 7.2) and resuspended in sterile-filtered ice-cold FACS buffer (2% (w/v) bovine serum albumin fraction V (Roche, Switzerland) and 0.01% sodium azide (w/v ...
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bioRxiv - Molecular Biology 2024Quote: ... and by qPCR using KAPA Library Quantification Kit for Illumina® Platforms (Roche). Library size was determined with Agilent Bioanalyzer 2100 system using the Agilent High Sensitivity DNA Kit ...
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bioRxiv - Molecular Biology 2024Quote: ... and by qPCR using KAPA Library Quantification Kit for Illumina® Platforms (Roche). Library size was determined with Agilent Bioanalyzer 2100 system using the Agilent High Sensitivity DNA Kit ...
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bioRxiv - Microbiology 2024Quote: ... on the LightCycler 480 System (Roche) with the following primers (5’ to 3’ ...
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bioRxiv - Neuroscience 2024Quote: ... Blood glucose levels were quantified using a hand-held glucometer (Roche, Accu-Check Compact). For triglyceride measurements ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM DTT added with protease inhibitor cocktail Complete EDTA-free (Roche) and lysed using sonicator ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM DTT added with protease inhibitor cocktail Complete EDTA-free (Roche) and lysed using sonicator ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed with cold PBS and lysed in IP lysis buffer supplemented with protease inhibitors (Roche, Basel, Switzerland, #05892791001). The supernatant was obtained by centrifugation at 10000 × g for 10 min at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mg of nuclear extract of KDM3A and KDM3B endogenously tagged ESCs (prepared as above) was incubated with 10 µg of HA antibody (Roche, clone 12ca5, catalog#11583816001) for 4 hours rotating at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM DTT added with protease inhibitor cocktail Complete EDTA-free (Roche) and lysed using sonicator ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM EDTA and 1% Triton X-100) containing 2 mM Na3VO4 (Sodium Orthovanadate) and 1 mM pmsf (phenylmethylsulfonyl fluoride) and 1x protease inhibitor cocktail tablet (Roche). Whole cell lysates were centrifuged at 12,000 rpm at 4 0C for 10 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... and the adipocytes were lysed using PBS containing 2% SDS and protease inhibitor (1 tablet per 10 mL; cOmplete Mini, Roche Diagnotics). Cell lysates were centrifuged at 14000 rpm for 10 minutes at 4 0C and total protein contents were measured by Bradford protein assay ...
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bioRxiv - Neuroscience 2024Quote: ... 0.1% SDS and 125mM sucrose) supplemented with Phos-STOP and protease inhibitors (Roche). A BCA Protein Assay kit (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... nmbb and nts probes (11175025910, Roche) and fluorescein (FITC)-labeled in vitro transcription of rln3a and sst1.1 probes (11685619910 ...
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bioRxiv - Neuroscience 2024Quote: ... Whole mount larvae and adult brain sections layered on glass slides were blocked for at least one hour in PBT with 2 mg/ml bovine serum albumin and 2% sheep serum at room temperature and then incubated overnight at 4°C with alkaline phosphatase-coupled anti-DIG antiserum (11093274910, Roche) diluted 1/5000 in blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... and 250 mg or 500mg protein was digested with 20 µg/ml proteinase K (PK) (Roche) for PrPSc blots for 1 hour at 37C ...
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bioRxiv - Cancer Biology 2024Quote: ... we treated sensitive and resistant cells with increasing concentrations of SP600125 for 24 hours and evaluated the cell viability using the Cell Proliferation Kit I (MTT) (Roche, Cat. 11465007001) and measuring the absorbance value at λ=590nm ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was assessed using the Cell Proliferation Kit I (MTT) (Roche, Cat. 11465007001), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... Libraries were quantified by real-time PCR using the KAPA Library Quantification Kit for Illumina Sequencing Platforms (Roche, Basel, Switzerland), followed by quality control using a DNA 100 Chip on a Bioanalyzer 2100 ...
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bioRxiv - Cell Biology 2024Quote: ... 1X protease inhibitors (Roche), 1X phosphatase inhibitor cocktail 1 (Sigma) ...
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bioRxiv - Cancer Biology 2024Quote: ... slides were blocked with digoxigenin blocking reagent (Roche, Mannheim, Germany) in maleic acid buffer containing 2% sheep serum at ambient temperature for 60 min and then incubated with sheep anti-digoxigenin conjugated to ALP (diluted 1:800 in blocking reagent ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 μL of 2× Sybr Green (LightCycler 96, Roche Molecular Systems, Inc.), 200 nM of each primer ...
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bioRxiv - Neuroscience 2024Quote: ... 50 μg/ml Insulin (Roche), 25 ng/ml bFGF (Corning ...
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bioRxiv - Biochemistry 2024Quote: ... chambers were treated with 1.5 mg/ml Blocking Reagent (Roche) for 30 min before introducing hSSB1 ...
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bioRxiv - Biochemistry 2024Quote: ... supplemented with Protease Inhibitor Cocktail (Roche) and disintegrated by sonication ...
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bioRxiv - Cell Biology 2024Quote: ... and one complete EDTA-free protease inhibitor mixture tablet (Roche Diagnostics). The lysates were centrifuged at 20,000×g for 30 min at 4 °C to obtain the soluble protein fraction containing the expressed LZTR1 proteins ...