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Citations for Roche :
1701 - 1750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and 1x Protease inhibitor cocktail (34044100, Roche Diagnostics GmBH). Cells were lysed by spinning at 70000 x g for 1 hour at 4°C and collecting the supernatant ...
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bioRxiv - Cell Biology 2024Quote: ... plus protease inhibitors (cOmplete, Roche, PMSF, Sigma) on ice ...
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bioRxiv - Developmental Biology 2024Quote: ... qPCR was performed with the Roche LightCycler 96 using FastStart Essential DNA Green Master (Roche; 06402712001). As reference gene we used the housekeeping gene Rplp0 ...
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bioRxiv - Developmental Biology 2024Quote: ... + 5% SDS + 2x cOmplete protease inhibitor cocktail (Roche). After centrifugation at 14k rpm for 10 min at RT ...
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bioRxiv - Cell Biology 2024Quote: ... DNA was stained with DAPI solution (Roche). Antibody details are provided in Table S3.
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bioRxiv - Cell Biology 2024Quote: ... HA (rat; Roche), Sec31 (mouse ...
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bioRxiv - Cell Biology 2024Quote: ... 1x EDTA-free complete protease inhibitor cocktail (Roche)] and lysis was initiated by addition of TritonX-100 to 0.1 % ...
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bioRxiv - Cell Biology 2024Quote: ... 1x complete protease inhibitor cocktail (Roche)] ...
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bioRxiv - Cell Biology 2024Quote: ... or DLD1 cells: anti-HA (Rat, Roche #ROAHAHA, 1:500), anti-SAS-6 (Mouse ...
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bioRxiv - Cell Biology 2024Quote: ... washed twice with PBS containing Complete Mini Protease Inhibitor Mixture (Roche Applied Science), resuspended in Laemmli buffer containing 1 mm DTT ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was quantified by qPCR with FastStart Essential DNA Green Master Mix (Roche, Ltd., Germany) and a real-time PCR instrument LightCycler (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... We used the Agilent Bioanalyzer to assess the quality of the library and a qPCR approach with the KAPA Library Quantification Kit (Roche; P/N: KK4873) on the QuantStudio 12K device to quantify the library ...
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bioRxiv - Developmental Biology 2024Quote: ... purified through a column (Roche), and final concentrations were measured using a Nanodrop 3300 spectrometer (Thermoscientific) ...
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bioRxiv - Developmental Biology 2024Quote: Primary tissue samples from reduction mammoplasties of healthy women were dissociated through a 3 mg/mL collagenase (Roche Life Science) solution and sorted via density gradient ...
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bioRxiv - Developmental Biology 2024Quote: ... The pIEx-4-BmU6-Cad96ca-gRNA-Cas9-GFP-P2A-Puro or pIEx-4-BmU6-Fgfr1-gRNA-Cas9-GFP-P2A-Puro recombinant vectors were transfected into HaEpi cells with transfection reagent (Roche, Basel, Switzerland). After 48 h of vector transfection (cells can be observed to express green fluorescent protein) ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were incubated in a blocking solution (0.5% Roche Western Blocking Reagent and 5% inactivated horse serum in TNTx ...
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bioRxiv - Developmental Biology 2024Quote: ... the tail of each embryo was collected for genomic DNA extraction using the KAPA Mouse Genotyping Kit HotStart (Kapa Biosystems, KK7352). Subsequently ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR products with flanking T7 promoters were used as template for the synthesis of anti-sense riboprobes using a T7 RNA polymerase (Roche) (Mercurio et al ...
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bioRxiv - Developmental Biology 2024Quote: ... The remaining lysate was rotated overnight with 50 µl of Protein A-agarose beads (Roche) and 2 µg of mouse anti-HA (monoclonal ...
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bioRxiv - Developmental Biology 2024Quote: ... EDTA-free Protease Inhibitor Cocktail (Roche, 11836170001). The resulting cell lysates underwent brief sonication and centrifugation ...
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bioRxiv - Developmental Biology 2024Quote: ... Incubation with anti-digoxigenin-AP Fab antibody (Roche), 1:2000 in PBST ...
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bioRxiv - Developmental Biology 2024Quote: ... and a digoxigenin labeling mix (Roche, catalog#: 11277073910). The synthesized riboprobes were treated with DNase I digestion to remove the DNA template ...
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bioRxiv - Developmental Biology 2024Quote: ... Animals were incubated with an anti-DIG-POD antibody (1:1,500; Roche) or anti-DNP-HRP (1:10,000 ...
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bioRxiv - Cell Biology 2024Quote: ... The chromosome V was visualized with a biotin-labelled probe generated by amplifying the endogenous 5s rDNA locus by PCR and labelling was performed with the Biotin-nick translation mix (Roche) according with manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: ... solution containing 1 μM of Y27362 (Roche), washed and digested for an additional 30 minutes in 0.25% trypsin (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... EDTA-free Protease Inhibitor Cocktail (Roche –11836170001).
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bioRxiv - Cell Biology 2024Quote: ... Cells were scraped into Laemmli buffer containing protease inhibitor cocktail (Roche) and pelleted to remove debris ...
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bioRxiv - Cell Biology 2024Quote: ... and KAPA qPCR (Kapa Biosystems KK4824), cDNA were sequenced on an Illumina HiSeq4000 at UCI’s Genomics Research and Technology Hub to achieve an average of 50,000 reads per cell.
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bioRxiv - Cell Biology 2024Quote: ... 50 µg of cryoground tissue powder was placed in a 1.5mL lobind tube and brought to 1 mL with lysis buffer (1% triton, 1x PBS, 1x Roche Complete protease inhibitor cocktail ...
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bioRxiv - Cell Biology 2024Quote: Apoptosis assay was performed using Annexin V FLUOS staining kit (Roche), and the manufacturer’s instructions were followed with slight modifications ...
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bioRxiv - Cell Biology 2024Quote: ... 1x EDTA-free complete protease inhibitor cocktail (Roche)] ...
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bioRxiv - Cell Biology 2024Quote: ... 1x EDTA-free complete protease cocktail (Roche)] and stored at -20°C.
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bioRxiv - Cell Biology 2024Quote: ... and a sgRNA preceded by a rrk1 leader and followed by a Hammerhead Ribozyme as described in [17] was digested and prepared for gap repair as follows: the vector pJB166 was purified from DH5α bacterial cells using the Genopure plasmid midi kit (Roche, 03143414001) and diluted to 1 μg/μL ...
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bioRxiv - Cell Biology 2024Quote: ... media containing 24 μL of X-tremeGENE 9 DNA (Roche) and added to HEK cells ...
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bioRxiv - Cell Biology 2024Quote: ... in Buffer D (20 mM HEPES pH 7.9, 150 mM KCl, 20% glycerol, 0.2 mM EDTA, 0.2% Triton X-100 and complete Roche inhibitor) overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... 100 synchronized young adult worms (24h post-L4 stage) were picked into 30 μl of 1XTE (Tris-EDTA Buffer, pH=8) containing 1X complete protease inhibitor cocktail (Roche) and flash-frozen in liquid nitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then digested for 2h in 1mg/ml Collagenase A (Roche, Basel, Switzerland) solution containing 1 μM of Y27362 (Roche) ...
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bioRxiv - Cell Biology 2024Quote: Cell lysates from mouse tissues were prepared in RIPA buffer (Beyotime, P0013B) containing Protease Inhibitor Cocktail (Roche, 11873580001) and 1mM PMSF ...
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bioRxiv - Immunology 2024Quote: ... The digestion was stopped by adding one volume of 2X STOP Buffer (340mM NaCl, 20mM EDTA, 4mM EGTA, 0.02% Digitonin, 50ug/mL RNase A (Cat #3335399001, Roche), 50ug/mL Linear Acrylamide (Cat #K548 ...
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bioRxiv - Immunology 2024Quote: ... 12,5 μg/ml DNAse (Roche), and 1% FBS (Bodego ...
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bioRxiv - Immunology 2024Quote: ... Whole transcriptome amplification was carried out using KAPA HiFi PCR Mastermix (Kapa Biosystems KK2602) with 2000 beads per 50-μl reaction volume ...
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bioRxiv - Immunology 2024Quote: ... cDNA libraries were then prepared from RNA samples using the KAPA-stranded mRNA-Seq kit (Roche KK8421). The sequencing was performed using the Illumina NovaSeq 6000 system ...
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bioRxiv - Immunology 2024Quote: ... DNase 12.5 U mL−1 (Roche, Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... and 10 μg/mL DNase I (Roche, Cat. 11284932001) for enzymatic digestion ...
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bioRxiv - Immunology 2024Quote: ... Tumours were dilacerated using scalpels to obtain <1mm sized pieces and resuspended in R10 supplemented with 1 mg/mL Liberase TL (Roche, Cat. 5401020001) and 10 μg/mL DNase I (Roche ...
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bioRxiv - Immunology 2024Quote: ... The purified libraries were quantified with Kapa Library Quant Kit (KAPA Biosystems) and quality assessed on a 4200 TapeStation System (Agilent) ...
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bioRxiv - Immunology 2024Quote: ... Quantitative real-time PCR was performed using FastStart Universal SYBR Green master (Roche) and run on LightCycler 96 Systems (Roche) ...
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bioRxiv - Immunology 2024Quote: Visceral white adipose tissues were diced and digested with 0.2 mg/ml Liberase TL (Roche) and 0.5 mg/ml DNase I (Sigma ...
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bioRxiv - Immunology 2024Quote: ... and digested for 1 hour at 37°C in 94 μg/mL DNase I (Roche) and 250 μg/mL collagenase type I and 250 μg/mL collagenase type IV (Worthington Biochemicals) ...
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bioRxiv - Immunology 2024Quote: ... The other 18 popliteal LNs were digested with 0.25 mg of Liberase DL (Roche, Indianapolis, IN) per mL of EHAA media with DNAse 1(Worthington ...