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Citations for Roche :
1801 - 1850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... or 1/5th volume of anti-GFP antibody (Roche) and 50μl of 0.1M Na-phosphate with gentle agitation for 30min at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... For pulldown experiments mouse brain extracts were obtained by homogenizing four brains in homogenization buffer (20 mM HEPES, 320 mM sucrose, pH 7.5) containing complete EDTA-free protease inhibitor cocktail (Roche). The homogenate was centrifuged at 1000 x g for 15 min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... anti-GFP (1:3000, Roche, cat # 1814460001) and anti-Flag (1:3000 ...
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bioRxiv - Cell Biology 2024Quote: ... two steps of transfections were performed: DNA using XtremeGENE™ 9 (Roche) followed by siRNA (4392420-s30722 ...
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bioRxiv - Immunology 2024Quote: ... Transcriptome libraries were generated using the KAPA RNA HyperPrep (Roche) using a poly-A selection (Thermo Scientific) ...
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bioRxiv - Immunology 2024Quote: ... quantified using the Qubit 3.0 Fluorometer as well as by quantitative PCR (KAPA Biosystems, Wilmington, MA, USA), and sequenced on a partial S4 lane of an Illumina NovaSeq 6000 with a paired-end ...
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bioRxiv - Immunology 2024Quote: ... and by qPCR using the KAPA Library Quantification Kit for Illumina (Roche, Basel, Switzerland). Libraries were pooled and sequenced on the Illumina NovaSeq 6000 (Illumina ...
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bioRxiv - Immunology 2024Quote: ... and DNAse-I (Roche). Samples were filtered through a 100 µm filter to obtain a single cell suspension ...
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bioRxiv - Genetics 2024Quote: ... qRT-PCR was performed in technical duplicate or triplicate using SYBR FAST qPCR Master Mix (Kapa Biosystems, Cape Town, South Africa) on a LightCycler 480 (Roche Applied Science ...
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bioRxiv - Genomics 2024Quote: ... Libraries were constructed with KAPA™ RNA HyperPrep with RiboErase (Roche, Indiana, USA) and performed based on manufacturer’s recommendations ...
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bioRxiv - Genomics 2024Quote: ... Cell pellets were resuspended in 250 µL ice-cold Hi-C lysis buffer (10 mM Tris-HCl pH 8.0, 10 mM NaCl, 0.2% Igepal CA630, 1 tablet/10 mL Roche complete mini EDTA-free protease inhibitor) ...
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bioRxiv - Genomics 2024Quote: ... and SeqCapAdapter Kit A (Roche, USA). 14 PCR cycles were used to amplify the libraries ...
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bioRxiv - Genomics 2024Quote: ... adapter ligation and PCR amplification was performed using the Kapa Hyper Prep kit (Roche). Beads were resuspended in 60 µL of a cocktail containing 50 µL H2O ...
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bioRxiv - Evolutionary Biology 2024Quote: We amplified the enriched products using the KAPA HiFi (2x) HotStart ReadyMix PCR Kit (Roche, Basel, Switzerland) for 10 cycles ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We used 25 μl KAPA HiFi HotStart ReadyMix (Kapa Biosystems), 5 μl of 5uM Illumina TruSeq iTru5 and iTru7 dual-indexed primers 82 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 300 mM Guanidine Hydrochloride plus cOmplete protease inhibitor (Roche). The cell suspension was lysed using a glass homogenizer followed by sonication (with 8 pulses of 10 second with 70% amplitude sonic vibrations ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The samples were de-frosted in 1 mL of 1x DpnII buffer with protease inhibitors (Roche cOmplete™), transferred to Precellys VK05 lysis tubes (Bertin Technologies ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We did PCRs in a reaction volume of 25 μl containing 12.5 μl 2x FastStart PCR master mix (Roche, Germany), one forward and two reverse MHC IIB primers (Bracamonte et al. ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Using Kapa HyperPrep kits (Kapa Biosystems) and Illumina TruSeq iTru5 and iTru7 adapters 82 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Protease inhibitor cocktail tablet (Roche) and 1 mM PMSF) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Protease inhibitors were then added (Roche). Quantification of protein concentration was done using a BCA Protein assay Kit (Thermo Fisher Scientific) ...
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The transcriptomic landscape of monosomy X (45,X) during early human fetal and placental developmentbioRxiv - Genetics 2024Quote: ... RNA was used to generate cDNA libraries using a KAPA mRNA HyperPrep Kit (Roche, Basel, Switzerland) on a Hamilton Starlet robotic platform (Hamilton Company ...
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bioRxiv - Developmental Biology 2024Quote: ... 3.5µL/mL NBT (Sigma-Roche, Cat # 11383213001) plus 3.5µL/mL BCIP (Sigma-Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.5% Sodium deoxycholate to which was added one tablet per 50 uL of protease inhibitor cocktail (cOmplete ULTRA Tablets, Mini, EDTA-free, EASY pack, Roche, REF 05 892 791 001)] or JUME Buffer [10 mM MOPS ...
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bioRxiv - Developmental Biology 2024Quote: ... blocked for 30 minutes in 1X Western Blocking Reagent (WBR, Roche #) in PBT at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were lysed in cold lysis buffer (10mM Tris-HCl pH7.5, 10mM NaCl, 5mM MgCl2, 1mM EGTA, with Roche Protease Inhibitor #04693159001) and incubated on ice for 10 minutes to extract nuclei ...
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bioRxiv - Developmental Biology 2024Quote: ... supplemented with a protease inhibitor cocktail (PIC, 11873580001, Roche, Switzerland). After centrifugation at 15,000 xg for 10 min at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... All riboprobes were generated from linearized plasmids and synthesized using the DIG RNA Labelling Kit (Roche, 11175025910).
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bioRxiv - Developmental Biology 2024Quote: ... The following day unbound chromatin was removed through seven washes in RIPA buffer (1% NP-40, 0.7% Na-Deoxycholate, 1mM EDTA, 50mM HEPES-KOH, pH 7.55 and 0.5M LiCl with Roche Protease Inhibitor #04693159001) and one in TE buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... with cOmplete™ Mini Protease Inhibitor Cocktail (Roche: 4693124001). Protein samples were boiled for 10 minutes at 95 °C and loaded onto Mini-PROTEAN TGX™ Precast Gels (BIO-RAD:4569036) ...
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bioRxiv - Developmental Biology 2024Quote: ... NBT/BCIP (4-nitro blue tetrazolium chloride/5-bromo-4-chloro-3-indolylphosphate, Roche, 11681451001) was added after thoroughly washing samples ...
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bioRxiv - Developmental Biology 2024Quote: ... and used to produce the DIG-labeled single-stranded RNA probes using the DIG RNA labeling kit (Roche, #11175025910). Probes were then cleaned with MegaClear Kit (Thermo Fisher ...
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bioRxiv - Developmental Biology 2024Quote: ... The probes were detected with AP coupled with anti-digoxigenin Fab fragments (Roche, 11093274910). AP reaction was developed with 4-Nitro blue tetrazolium chloride (NBT ...
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bioRxiv - Developmental Biology 2024Quote: ... in 1% blocking regent (Roche, 1096176)/PBS ...
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bioRxiv - Developmental Biology 2024Quote: Pitx1 WISH was performed on E12.5 embryos with a digoxigenin-labelled Pitx1 antisense probe designed from a cloned antisense probe (PCR DIG Probe Synthesis Kit, Roche 11636090910). Experimental procedure followed the protocol outlined in (Kragesteen et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were then resuspended in cold lysis buffer (10 mM Tris, pH 7.5, 10 mM NaCl, 5 mM MgCl2, 1 mM EGTA, Protease Inhibitor (Roche, 04693159001)) ...
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bioRxiv - Developmental Biology 2024Quote: ... the samples were incubated overnight with Streptavidin-POD conjugate (Roche, 1:500 dilution) and then washed ...
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bioRxiv - Genetics 2024Quote: ... cut to very small pieces using a scalpel blade and digested using Collagenase D (Roche, Cat No. 11088858001, 1 mg/ml) /DNase I (Thermo Fisher ...
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bioRxiv - Genetics 2024Quote: ... qPCR was performed using a LightCycler® 96 Instrument (Roche) and SsoFast™ EvaGreen® Supermix with Low ROX (Bio RAD #1725211 ...
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bioRxiv - Genetics 2024Quote: ... were run with molecular weight standards Marker VIII (Roche) at 100V for 60 min in 1X TBE ...
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bioRxiv - Developmental Biology 2024Quote: ... Fasting blood glucose was measured at the end of treatment after a 12-hr fast using a single tail nick bleed to measure blood glucose levels via glucometer (Cat# 68623221868, Accu-Chek Performa, Roche). Perigonadal white adipose tissue (PWAT ...
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bioRxiv - Developmental Biology 2024Quote: ... then hybridized overnight at 65 °C with digoxigenin-labeled RNA probes (Roche, 11277073910). The probes were detected with AP coupled with anti-digoxigenin Fab fragments (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... AP reaction was developed with 4-Nitro blue tetrazolium chloride (NBT, Roche, 11383213001) and 5-bromo-4-chloro-3’-indolyphosphate p-toluidine salt (BCIP ...
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bioRxiv - Developmental Biology 2024Quote: ... Hematoxylin and eosin staining was performed using a Ventana Discovery Ultra automated slide preparation system (Roche).
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bioRxiv - Developmental Biology 2024Quote: ... Quantitative PCR (qPCR) was performed using the KAPA SYBR Fast qPCR Master Mix (KAPA Biosystems) on the StepOnePlus Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2024Quote: ... A 20 µl reaction (DIG RNA labelling mix (Roche), T7 RNA Polymerase (Roche) ...
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bioRxiv - Developmental Biology 2024Quote: ... 10X Transcription Buffer (Roche), Ribo-Lock RNase inhibitor (Thermo Fisher) ...
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bioRxiv - Developmental Biology 2024Quote: ... rat anti-HA (3F10; Roche Applied Science; RRID:AB_2314622), mouse anti-V5 (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... and protease inhibitor cocktail (Complete, Roche) were added ...
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bioRxiv - Developmental Biology 2024Quote: ... T7 RNA Polymerase (Roche), 10X Transcription Buffer (Roche) ...