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Citations for Roche :
1601 - 1650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... PCR was performed with KAPA HiFi DNA polymerase (Kapa Biosystems cat. KK2502) using barcoded indexing primers for Illumina ...
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bioRxiv - Genomics 2024Quote: ... and finally quantified by qPCR (Kapa Biosystems, Wilmington, MA, USA). These libraries were sequenced with 150 bp paired-end reads on an Illumina HiSeq 4000 or NovaSeq 6000 instrument (Illumina ...
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bioRxiv - Genomics 2024Quote: ... Adapted fragments were amplified by 12 cycles of PCR using the Kapa Hifi Hotstart NGS library Amplification kit (Roche, Basel, Switzerland), followed by 0.8x AMPure XP (Beckman Coulter Genomics ...
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bioRxiv - Genomics 2024Quote: ... as well as a Kapa library quantification kit for Illumina libraries (Roche, Switzerland), were used ...
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bioRxiv - Genomics 2024Quote: Library pools were amplified using KAPA HiFi Hotstart Readymix (Roche KK2601) with 0.3 µM each oJL109 and oJL090 and 20 ng input DNA per 50 µL PCR ...
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bioRxiv - Genomics 2024Quote: Real-time quantitative PCR reactions were performed using a LightCycler 480 system (Roche) with SYBR Green 2× PCR Master Mix I (Roche ...
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bioRxiv - Genomics 2024Quote: ... Samples were diluted in low-TE buffer and were subjected to 3 different miniaturized library prep methods: KAPA HyperPlus (Roche, KK8514), DNA Prep (Illumina ...
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bioRxiv - Genomics 2024Quote: ... the cDNA libraries were quantified using the KAPA Library Quantification kit (KR0405-v11.20, Kapa Biosystems, Woburn, USA). Paired-end reads of 150 bp were generated using TruSeq RNA Access sequencing chemistry on HiSeq X instrument (Illumina ...
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bioRxiv - Genomics 2024Quote: ... The cleared lysate was loaded onto a cOmplete His-tag purification column (Roche) and the His6-Sumo3-Tn5 was eluted with a running buffer containing 300 mM imidazole ...
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bioRxiv - Genomics 2024Quote: ... designed with Roche NimbleDesign online tool (Roche, now HyperDesign ...
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bioRxiv - Genomics 2024Quote: ... and 10 % glycerol) supplemented with cOmplete protease inhibitors (Roche) and lysed using a Microfluidizer ...
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bioRxiv - Genomics 2024Quote: ... Hybridization was carried out with SeqCap RNA Choice Probes using the KAPA HyperCapture Reagent and Bead kit (v2, Roche Sequencing Solutions, Inc.) according to SeqCap RNA Enrichment System User’s Guide (v.1.0 ...
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bioRxiv - Genomics 2024Quote: ... and 18.8 % dimethylformamide supplemented with 0.005x protease inhibitor cocktail (Roche, catalog no. 11697498001), 0.4 U/µl SUPERaseIn (Thermo Fisher ...
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bioRxiv - Genomics 2024Quote: ... 1 U/µl Protector RNase inhibitor (Roche, catalog no. 3335402001), 0.2 U/µl SUPERaseIn (Thermo Fisher ...
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bioRxiv - Genomics 2024Quote: ... DNA was extracted using the MagNA Pure DNA isolation kit (Roche Molecular Systems ...
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bioRxiv - Genomics 2024Quote: ... Both FFPE extracted DNA and PBMC DNA Libraries were prepared using KAPA HyperPlus Kit (Roche) following the recommended protocol ...
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bioRxiv - Immunology 2024Quote: ... minced and digested for 30 min in collagenase D (2 mg/mL, Roche) and DNase I (100 μg/mL ...
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bioRxiv - Genomics 2024Quote: ... and 0.1% Tween-20 (Roche, 11332465001) in 1x divalent cation-free phosphate-buffered saline ...
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bioRxiv - Genomics 2024Quote: ... All hybridization reagents were purchased from Roche as part of the KAPA Hyper Prep kit ...
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bioRxiv - Genomics 2024Quote: ... Amplified libraries were subjected to hybridization with biotinylated oligonucleotide pools that were designed by the HyperDesign Team (Roche) and synthesized by Roche ...
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bioRxiv - Genomics 2024Quote: ... we used bisulphite-converted product as input in PCR reactions and tested an annealing temperature gradient ranging from 55 to 62C for 30 cycles with Kapa Uracil+ HotStart ReadyMix polymerase (Roche, 7959052001). Samples of each PCR product were run on a 2% agarose gel ...
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bioRxiv - Genomics 2024Quote: ... and synthesized by Roche. All hybridization reagents were purchased from Roche as part of the KAPA Hyper Prep kit ...
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bioRxiv - Genomics 2024Quote: ... using the Biotin and Dig Nick Translation Mix (Roche, Mannheim, Germany). Chromosome preparations were made from fibroblast cell culture as described previously61 ...
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bioRxiv - Genomics 2024Quote: ... and 22-bp Sat III were synthesized as probes and labeled with Dig-dUTP or Bio-dUTP (Roche Diagnostics, Basel, Switzerland) using the Biotin and Dig Nick Translation Mix (Roche ...
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bioRxiv - Genomics 2024Quote: ... and HTO were performed in 1X KAPA HiFi PCR reagent (Roche) with 1 µM of forward primer and 1 µM of reverse primer and thermal protocol as specified below ...
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bioRxiv - Genomics 2024Quote: ... Proteinase K (100 mg/ml, Roche) was then added ...
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bioRxiv - Genomics 2024Quote: ... and libraries were constructed using on-bead PCR amplification using the KAPA HyperPlus kit (Roche). The constructed libraries were then sequenced using the Illumina NovaSeq 6000 platform (paired-end 50 bp reads ...
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bioRxiv - Immunology 2024Quote: ... digested three times (DMEM high glucose with 2% FBS, 100 µg/ml DNase I, and 100 µg/ml Liberase TM, Roche) or until no tissue fragments remained ...
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bioRxiv - Immunology 2024Quote: ... and 1.0% Nonidet P-40) containing protease and phosphatase inhibitors (PMSF, NaF, Na3VO4, and Roche PhosSTOP). Lysates were cleared by centrifuging at 10,000 g for 10 min at 4° C ...
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bioRxiv - Immunology 2024Quote: ... and subsequent PCR with the KAPA HiFi HotStart Ready Mix (Roche) and ISO SMART primer as per the standard protocol ...
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bioRxiv - Immunology 2024Quote: ... Libraries were quantified by qPCR measured by Kapa Library Quantification kit (Kapa Biosystems, Woburn, MA) using ABI’s 9700HT real-time PCR system (Lifetech ...
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bioRxiv - Genomics 2024Quote: ... Oligo pool amplification PCRs were assembled with 1X KAPA HiFi HotStart Ready Mix (Roche KK2601), 1X EvaGreen qPCR dye (Biotium 31000) ...
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bioRxiv - Genetics 2024Quote: ... supplemented with 1X Protease Inhibitor Cocktail (Roche) and then centrifuged for 10 min at 13,200 rpm to remove cell debris.
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bioRxiv - Genomics 2024Quote: ... cDNA was synthesized with 200ng of the stored RNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche, 04897030001) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... and supplemented with pronase (0.3 µg/ml, Roche, #10165921001). Cartilage fragments were digested in this medium for 90 minutes at 37°C with gentle shaking ...
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bioRxiv - Genetics 2024Quote: ... the SYBR Green I Master mix was used on a LightCycler 480 Real Time PCR System (Roche). The primers are listed in Table S2 ...
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bioRxiv - Genetics 2024Quote: ... 0.5% NP-40) supplemented with Complete Protease inhibitor cocktail (Roche, 11697498001), Complete Phosphatase inhibitor cocktail (Roche ...
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bioRxiv - Genomics 2024Quote: ... DNA was isolated following standard KAPA Pure Beads (KAPA Biosystems Catalog# KK8000) protocol with a 1.5X ratio of beads to DNA ...
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bioRxiv - Genomics 2024Quote: ... Cells were then transfected with the Tet-pLKO-puro plasmids targeting either UHRF1 or DNMT1 transcripts and the accompanying lentiviral packaging (psPAX2) and envelope (pMD.2G) plasmids with Opti-MEM and Xtreme HP Gene transfection reagent (Roche) per the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... and Kapa Illumina Library Quantification qPCR assays (Kapa Biosystems). Individually indexed libraries were pooled and 50 bp ...
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bioRxiv - Genomics 2024Quote: ... and protease inhibitor cocktail (Roche cOmplete Mini tablets, EDTA-free)) on ice and centrifuged to remove insoluble components ...
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bioRxiv - Genomics 2024Quote: ... Protease inhibitor cocktail (Roche cOmplete Mini tablets, EDTA-free)) and incubated for 10 minutes rotating at 4°C ...
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bioRxiv - Genomics 2024Quote: ... with SYBR Green 2× PCR Master Mix I (Roche, Cat# 04887352001) and 1 μM of forward and reverse primer respectively ...
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bioRxiv - Genomics 2024Quote: ... 25× protease inhibitor cocktail (Roche), 1:1000 dilution of SUPERase In™ RNase (Invitrogen ...
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bioRxiv - Genomics 2024Quote: ... Miniaturization involved testing libraries at 1/6th (IDT mini) or 1/8th (Roche mini, Illumina mini) of reaction volume tailoring input DNA to each kit between 24-45 ng total (Table 2) ...
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bioRxiv - Genomics 2024Quote: ... the dialyzed sample was loaded again onto a cOmplete His-tag purification column (Roche), and the untagged Tn5 was collected in the flow-through ...
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bioRxiv - Genomics 2024Quote: ... cDNA was synthesized with 200ng of the stored RNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche, 04897030001) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... TMR red (Roche 12156792910) manufacturer’s protocol was followed to detect cell death via DNA fragmentation ...
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bioRxiv - Developmental Biology 2024Quote: ... plus 3.5µL/mL BCIP (Sigma-Roche, Cat # 11383221001) in the Staining buffer (0.1M Tris-HCl ...
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bioRxiv - Developmental Biology 2024Quote: ... 3.5µL/mL NBT (Sigma-Roche, Cat # 11383213001) plus 3.5µL/mL BCIP (Sigma-Roche ...