Labshake search
Citations for Roche :
1601 - 1650 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2024Quote: ... AP reaction was developed with 4-Nitro blue tetrazolium chloride (NBT, Roche, 11383213001) and 5-bromo-4-chloro-3’-indolyphosphate p-toluidine salt (BCIP ...
-
bioRxiv - Developmental Biology 2024Quote: ... Hematoxylin and eosin staining was performed using a Ventana Discovery Ultra automated slide preparation system (Roche).
-
bioRxiv - Developmental Biology 2024Quote: ... Quantitative PCR (qPCR) was performed using the KAPA SYBR Fast qPCR Master Mix (KAPA Biosystems) on the StepOnePlus Real-Time PCR System (Applied Biosystems) ...
-
bioRxiv - Developmental Biology 2024Quote: ... A 20 µl reaction (DIG RNA labelling mix (Roche), T7 RNA Polymerase (Roche) ...
-
bioRxiv - Developmental Biology 2024Quote: ... 10X Transcription Buffer (Roche), Ribo-Lock RNase inhibitor (Thermo Fisher) ...
-
bioRxiv - Developmental Biology 2024Quote: ... rat anti-HA (3F10; Roche Applied Science; RRID:AB_2314622), mouse anti-V5 (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2024Quote: ... and protease inhibitor cocktail (Complete, Roche) were added ...
-
bioRxiv - Developmental Biology 2024Quote: ... T7 RNA Polymerase (Roche), 10X Transcription Buffer (Roche) ...
-
bioRxiv - Developmental Biology 2024Quote: ... TMR red (Roche 12156792910) manufacturer’s protocol was followed to detect cell death via DNA fragmentation ...
-
bioRxiv - Developmental Biology 2024Quote: ... plus 3.5µL/mL BCIP (Sigma-Roche, Cat # 11383221001) in the Staining buffer (0.1M Tris-HCl ...
-
bioRxiv - Genetics 2024Quote: ... and 0.5% NP-40) supplemented with a protease inhibitor cocktail (cOmplete, Mini Protease Inhibitor Cocktail, Roche, Basel, Switzerland) and phosphatase inhibitors (PhosSTOP ...
-
bioRxiv - Genetics 2024Quote: ... and protease inhibitors (Complete, EDTA-free, Roche). Lysis was performed with 0.5 mm acid washed beads in a FastPrep instrument (MP ...
-
bioRxiv - Genetics 2024Quote: ... The pool was quantified by qPCR with a Kapa Library Quant kit (Kapa Biosystems) and sequenced on an Illumina NovaSeq 6000 (Illumina ...
-
bioRxiv - Genetics 2024Quote: ... Strand-specific and dual-barcode indexed RNA-seq libraries were generated from 450 ng total RNA each using the Kapa RNA-seq Hyper kit (Kapa Biosystems-Roche, Basel, Switzerland) and both the QIAseq FastSelect–5S/16S/23S ribodepletion and FastSelect rRNA Plant reagents (Qiagen ...
-
bioRxiv - Genetics 2024Quote: ... Strand-specific and dual-barcode indexed RNA-seq libraries were generated from 450 ng total RNA each using the Kapa RNA-seq Hyper kit (Kapa Biosystems-Roche, Basel, Switzerland) and both the QIAseq FastSelect–5S/16S/23S ribodepletion and FastSelect rRNA Plant reagents (Qiagen ...
-
bioRxiv - Genetics 2024Quote: ... 0.1% Na-Doc) with freshly added EDTA-free protease inhibitors (Roche), 1mM PMSF and 100 U/ml SuperaseIn (Invitrogen ...
-
bioRxiv - Genetics 2024Quote: ... the library was quantified by qPCR with a Kapa Library Quant kit (Kapa Biosystems/Roche, Basel, Switzerland). The library was sequenced on one flow cell of Aviti sequencer (Element Biosciences ...
-
bioRxiv - Developmental Biology 2024Quote: ... ovaries were crosslinked in 250 µL YSS buffer (50 mM pH 8.0 Tris HCl, 50 mM NaCl, 0.1% NP40, 1 U/mL of RNAsin (Roche, 3335402001), protease inhibitor (Thermo Scientific ...
-
bioRxiv - Developmental Biology 2024Quote: ... heads were rinsed for 5 minutes in PBST five times and incubated in Blocking Reagent (Roche, 1:5000 dilution) for 60 minutes ...
-
bioRxiv - Developmental Biology 2024Quote: ... Digoxygenin or biotin-labelled probes were synthesized using the labelling kit (Roche). The template DNA for the Osi10b RNA antisense probe was amplified with the primer set (Forward ...
-
bioRxiv - Genetics 2024Quote: ... the library was quantified by qPCR with a Kapa Library Quant kit (Kapa Biosystems/Roche, Basel, Switzerland). The library was sequenced on one flow cell of Aviti sequencer (Element Biosciences ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... The four paired-end libraries were quantified by qPCR using the KAPA Library Quantification Kit (Roche). Genome sequencing was conducted on the Illumina MiSeq platform at the Federal University of Rio Grande do Sul ...
-
bioRxiv - Developmental Biology 2024Quote: ... and 0.1% Sodium deoxycholate) supplemented with Protease Inhibitor Cocktail COMPLETE EDTA-Free (Roche # 11873580001) and Phosphatase Inhibitor Cocktail Set II (Millipore #524625 ...
-
bioRxiv - Developmental Biology 2024Quote: ... blocked in MAB-Buffer2 (20% sheep serum, MAB-Buffer1) for 1h and incubated with anti-DIG-AP antibody (1:4000, Roche) in MAB-Buffer2 overnight at 4 °C.
-
bioRxiv - Developmental Biology 2024Quote: ... were pre-washed in 0.25%BSA/DPBS and resuspended in 1ml of L3 sonication buffer (10mM TrisCl pH 8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% Na-Deoxycholate, 0.5% N-Laroylsarcosine, filtered and with Roche Protease Inhibitor #04693159001) with 1% Triton ...
-
bioRxiv - Developmental Biology 2024Quote: ... levamisole 1 mM) containing NBT/BCIP (Roche). The background colour was removed by a series of washes in EtOH/PBSTw (30%/70% ...
-
bioRxiv - Developmental Biology 2024Quote: ... digested with 10 μg/mL Proteinase K (PK, Roche) in 0.1% SDS ...
-
bioRxiv - Developmental Biology 2024Quote: ... Plasma was stored at −30°C for analysis of leptin and insulin (CrystalChem Inc., Chicago, IL, USA) and triglyceride content (Roche triglyceride reagent ...
-
bioRxiv - Developmental Biology 2024Quote: ... 28 using the KAPA Hyperprep Kit (KAPA Biosystems, Cat. #KK8504) with the following modifications ...
-
bioRxiv - Developmental Biology 2024Quote: ... The labelling efficiency of probes was tested by performing a dot plot following the protocol provided by Roche. The probes that exhibited strong signals in the dot plot were considered suitable for subsequent fluorescence in-situ hybridization ...
-
bioRxiv - Developmental Biology 2024Quote: Antisense digoxygenin-labeled probes (Genius kit; Roche, Indianapolis, IN) were synthesized using template cDNA encoding X ...
-
bioRxiv - Developmental Biology 2024Quote: ... The signal is developed in BM Purple (Roche 11442074001) in the dark and the signal checked regularly until the best possible signal-to-noise ratio is achieved ...
-
bioRxiv - Developmental Biology 2024Quote: ... and 0.1% sodium dodecyl sulfate) supplemented with 2% v/v protease inhibitor cocktail (Roche; Catalog #11697498001) and 1% v/v phosphatase inhibitor cocktail (Sigma-Aldrich ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Staining was developed in BM-Purple (Roche #11442074001) for approximately 4 hours at room temperature.
-
bioRxiv - Developmental Biology 2024Quote: ... DIG (digoxigenin) RNA labelling mix (Roche, Cat.No. 11277073910). According to the cloning direct of the insert ...
-
bioRxiv - Developmental Biology 2024Quote: ... A tablet of protease inhibitor cocktail (Roche) and a tablet of PhosSTOP were dissolved in 4.75ml water ...
-
bioRxiv - Developmental Biology 2024Quote: ... Libraries from the CUT&RUN fragments were made with a KAPA Hyper Prep kit (Roche). Samples were then gel purified and size selected using E-gel EX 2% Agarose (Invitrogen ...
-
bioRxiv - Developmental Biology 2024Quote: ... or the Fluorescein RNA labeling mix (Roche, Sigma product # 11685619910) to facilitate double staining.
-
bioRxiv - Developmental Biology 2024Quote: ... Probes were labelled with either the DIG RNA labeling mix (Roche, Sigma product # 11277073910) or the Fluorescein RNA labeling mix (Roche ...
-
bioRxiv - Developmental Biology 2024Quote: ... Libraries were prepared with the KAPA mRNA hyperprep kit (Roche). Samples were then gel purified and size selected using E-gel EX 2% Agarose (Invitrogen ...
-
bioRxiv - Developmental Biology 2024Quote: ... the heads were incubated in the mixture of Anti-Digoxigenin-POD (Roche, 1:500 dilution), anti-Futsch (DSHB ...
-
bioRxiv - Developmental Biology 2024Quote: ... 50 µL of Kapa Hifi Hotstart Ready Mix (Roche) and 40 µL of Nuclease-free water (Invitrogen) ...
-
bioRxiv - Developmental Biology 2024Quote: ... After post-hybridisation washed and blocking with BMB (Roche), embryos were treated with anti-DIG antibody coupled to alkaline phosphatase (Merck ...
-
bioRxiv - Developmental Biology 2024Quote: ... TMR Red (12156792910, Roche). Slides were fixed in 4% PFA ...
-
bioRxiv - Genetics 2024Quote: ... qPCR was performed using the KAPA SYBR FAST kit (KAPA Biosystems) and BioRad CFX96 Real-Time System ...
-
bioRxiv - Genetics 2024Quote: 12.5 Kapa HiFi HotStart PCR ReadyMix (KAPA Biosystems, KK2601)
-
bioRxiv - Genetics 2024Quote: ... and 15 μL of Kapa HiFi HotStart ReadyMix polymerase (Roche Diagnostics, KK2601). Each reaction tube was initially incubated at 95°C for 5 mins ...
-
bioRxiv - Genetics 2024Quote: ... 1 mM DTT, 10 mM NaPPi, 5 mM EGTA, 5 mM EDTA, 0.1 mM Na3VO4, 5 mM NaF, and Roche cOmplete™ Protease Inhibitor Mix). Crude extracts were prepared by centrifugation ...
-
bioRxiv - Genetics 2024Quote: ... and digested with NB4 collagenase (12mg/ml) (SERVA) and Dispase II (100U/ml) (Roche) for 1 hour ...
-
bioRxiv - Genetics 2024Quote: ... and phosphatase inhibitors (Roche, 04906837001). Lysis was allowed to proceed on ice for 30 minutes before centrifugation at 20,000xg for 10 min at 4°C ...