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Citations for Roche :
1751 - 1800 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... supplemented with Protease Inhibitor Cocktail (Roche) and disintegrated by sonication ...
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bioRxiv - Cell Biology 2024Quote: ... and one complete EDTA-free protease inhibitor mixture tablet (Roche Diagnostics). The lysates were centrifuged at 20,000×g for 30 min at 4 °C to obtain the soluble protein fraction containing the expressed LZTR1 proteins ...
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bioRxiv - Cell Biology 2024Quote: ... and the adipocytes were lysed using PBS containing 2 % SDS and protease inhibitor (1 tablet per 10 mL; cOmplete Mini, Roche Diagnotics). Secretion samples were centrifuged for 5 minutes at 2000 rpm and 4 0C ...
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bioRxiv - Cell Biology 2024Quote: Cells were collected in RIPA buffer containing 1x cOmplete EDTA-free protease inhibitor (Roche, 05056489001) and were then mechanically lysed by passage through a 27G needle on ice ...
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bioRxiv - Cancer Biology 2024Quote: Cells were lysed in RIPA buffer supplemented with a protease inhibitor cocktail (Roche) and phosphatase inhibitor (Cell Signaling 5870s) ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293T cells were co-transfected with sgRNA expression vectors and lentiviral packaging constructs psPAX2 and pMD2.G (VSV-G) in a 2:1:1 ratio using X-tremeGENE 9 DNA Transfection Reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... expression vectors were co-transfected into HEK293T cells with the lentiviral packaging constructs psPAX2 and pMD2.G (VSV-G) in a 1:1:1 ratio using X-tremeGENE9 DNA Transfection Reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and 2mg/mL collagenase (Roche). Following digestion and three subsequent PBS washes ...
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bioRxiv - Immunology 2024Quote: ... 0.5 mg/ml Collagenase IV (USBiological) and 30 µg/ml DNase I grade II (Roche) for 45 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... All lung lobes were cut into small pieces and digested in R5 media (RPMI 1640 supplemented with FCS (5 %) containing DNase I grade II (30 µg/ml) and Liberase TM (50 µg/ml, Roche) for 45 min at 37 °C with a magnetic stirrer.
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bioRxiv - Cell Biology 2024Quote: Embryonic lungs at E13.5 were collected in RIPA Lysis and Extraction Buffer (Thermo) supplemented with cOmplete Protease Inhibitor Cocktail tablets (Roche). Samples were homogenized in a QIAGEN TissueLyser II and proteins were purified from the supernatant after high-speed centrifuge ...
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bioRxiv - Cell Biology 2024Quote: ... with primer set #3 (Supplementary Table 1) using KAPA High-Fidelity DNA Polymerase (KAPA Biosystems, KE2502). The forward and reverse primers contained BspEI and KpnII sites respectively ...
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bioRxiv - Cell Biology 2024Quote: ... the full-length Treacle was amplified by PCR from cDNA with primer set #4 (Supplementary Table 1) using KAPA High-Fidelity DNA Polymerase (KAPA Biosystems, KE2502). The forward and reverse primers contained BglII and BamHI sites respectively ...
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bioRxiv - Cell Biology 2024Quote: ... the full-length Treacle was amplified by PCR from cDNA with primer set #1 (Supplementary Table 1) using KAPA High-Fidelity DNA Polymerase (KAPA Biosystems, KE2502). The forward and reverse primers contained XhoI sites ...
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bioRxiv - Cell Biology 2024Quote: ... 0.525mg/ml collagenase D (Roche), 5 unit/ml Dispase (Stemcell Technologies ...
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bioRxiv - Pathology 2024Quote: ... and detection were performed using DIG High Prime DNA Labeling and Detection Starter Kit I (Roche) according to the instruction manual.
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bioRxiv - Cell Biology 2024Quote: ... cDNA was then used for quantitative PCR (qPCR) done with SYBR Green Master Mix (Roche) and run in LightCycler 480 for 40 cycles ...
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bioRxiv - Cell Biology 2024Quote: ... and 0.25 mg DNase I (Roche) by running mouse lung 1-2 program on GentleMACS (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2024Quote: ... 0.525 mg/ml collagenase/dispase (Roche) and 0.25 mg DNase I (Roche ...
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bioRxiv - Immunology 2024Quote: ... with Protease Inhibitor cocktail (Roche) at the time of ELISPOT and lysates of all three experiments were analyzed together by Western blot ...
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bioRxiv - Immunology 2024Quote: ... plus 100 ug/ml Dnase (Roche) for 5 minutes ...
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bioRxiv - Immunology 2024Quote: ... the cells were quickly transferred into 10 ml of pre-warmed RPMI supplemented with 10% FBS and 100µg of Pulmozyme (dornase alpha, Roche, Basel, Switzerland) for 30 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... and the LightCycler 96 System (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... total nucleic acids (NA) were extracted with the automated MagNA Pure LC instrument (Roche) using the MagNA Pure LC DNA Isolation Kit – High performance (Roche ...
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bioRxiv - Microbiology 2024Quote: ... total RNA from fractionated cells was isolated with a High Pure RNA Isolation kit (Roche). (i ...
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bioRxiv - Microbiology 2024Quote: ... The amount of HSV-1 DNA was quantified using the Universal Probe Library (Roche) with TaqMan Master (Roche ...
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bioRxiv - Microbiology 2024Quote: ... with TaqMan Master (Roche) and the LightCycler 96 System (Roche ...
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bioRxiv - Microbiology 2024Quote: ... using the MagNA Pure LC DNA Isolation Kit – High performance (Roche, product no. 03310515001), and eluted in 50μL ...
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bioRxiv - Cancer Biology 2024Quote: qPCR reactions containing 1X KAPA SYBR Fast qPCR Master Mix (Roche), transcript-specific primers ...
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bioRxiv - Cancer Biology 2024Quote: ... The RT-qPCR cycling analysis was performed using a LC-480 device (Roche). qBasePlus software 3.2 (www.biogazelle.com ...
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bioRxiv - Bioengineering 2024Quote: ... EDTA-free Protease Inhibitor Cocktail (Roche) and 1 mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Biochemistry 2024Quote: ... an additional 6-cycle PCR was carried out with the HiFi HotStart Ready Mix (Kapa Biosystems, Wilmington, MA) and primers that preserve the DNA sequence ...
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bioRxiv - Developmental Biology 2024Quote: ... The expression of transcripts of the target gene was measured by using a LightCycle® 96 instrument (Roche) with Hieff UNICON SYBR green master mix (11198ES08 ...
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bioRxiv - Microbiology 2024Quote: ... and RNase A (Roche) to remove exogenous bacterial DNA and RNA prior to the addition of sodium dodecyl sulphate and proteinase K to release the DNA from phage particles ...
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bioRxiv - Genetics 2024Quote: ... and PCR amplified using the KAPA HiFi Uracil PCR Kit (Catalogue Number: ROC-07959052001, Kapa Biosystems). The final libraries were assessed with the Agilent 2200 Tapestation System using D1000 Kit (Catalogue Number:5067-5582) ...
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bioRxiv - Cancer Biology 2024Quote: ... and phosphatase inhibitors (PhosSTOP, Roche, 4906837001). Lysates were resolved by SDS-PAGE and transferred onto a nitrocellulose membrane (see above ...
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bioRxiv - Biophysics 2024Quote: ... coli MRE 600 (Roche), 227.5 μg/μL Folinic acid ...
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bioRxiv - Cell Biology 2024Quote: ... protease and phosphatase inhibitors (Roche). The lysates were centrifuged at 20,000 x g for 10 min at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... protease inhibitors (Roche 11836170001), RNAse inhibitor (Ambion AM2696) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1X PhosSTOP (Roche) and 0.5 mM Dithiothreitol (DTT ...
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bioRxiv - Cell Biology 2024Quote: ... the T17A3 cosmid was used to generate a digoxigenin-labelled DNA probe with the Digoxigenin-nick translation mix (Roche) according with manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: ... using SYBR Green I Master Mix (4887352001, Roche). Specific primers for ChIP-qPCR analysis are listed in Suppl ...
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bioRxiv - Immunology 2024Quote: ... 1 U HiFi enzyme mix (Roche), 1X buffer ...
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bioRxiv - Immunology 2024Quote: ... and 30 units/ml DNase (Roche) in PBS for 30min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... Primary antibodies dilutions were as follow: high affinity rat anti-HA (clone 3F10, Roche; 1:1,000), mouse anti-FIKK4.2 (1:1,000 ...
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bioRxiv - Neuroscience 2024Quote: ... 0.2 mg/ml BSA (Roche, 10711454001), 2× SSC ...
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bioRxiv - Microbiology 2024Quote: C3b was generated from C3 by limited proteolysis with trypsin (Roche) at 1 % w/w trypsin to C3 at 37°C for 2 minutes ...
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bioRxiv - Immunology 2024Quote: ... and 100µg/mL of DNAse I (Roche, #10104159001)) was added ...
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bioRxiv - Microbiology 2024Quote: ... The purified parasites were lysed in 200 μl RIPA buffer (150mM NaCl, 10 mM TrisHCl, pH 7.5, 0.1% SDS, 1% TX100 containing 2x protease inhibitor cocktail (Roche), and 1 mM PMSF ...
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bioRxiv - Microbiology 2024Quote: ... Primary antibodies were: monoclonal mouse anti-GFP (Roche) (1:1000) ...