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Citations for Roche :
1501 - 1550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... and 15 μL of Kapa HiFi HotStart ReadyMix polymerase (Roche Diagnostics, KK2601). Each reaction tube was initially incubated at 95°C for 5 mins ...
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bioRxiv - Genetics 2024Quote: ... 1 mM DTT, 10 mM NaPPi, 5 mM EGTA, 5 mM EDTA, 0.1 mM Na3VO4, 5 mM NaF, and Roche cOmplete™ Protease Inhibitor Mix). Crude extracts were prepared by centrifugation ...
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bioRxiv - Genetics 2024Quote: ... and digested with NB4 collagenase (12mg/ml) (SERVA) and Dispase II (100U/ml) (Roche) for 1 hour ...
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bioRxiv - Genetics 2024Quote: ... and phosphatase inhibitors (Roche, 04906837001). Lysis was allowed to proceed on ice for 30 minutes before centrifugation at 20,000xg for 10 min at 4°C ...
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bioRxiv - Genomics 2024Quote: ... and the RT-qPCR was performed using ChamQ SYBR qRCR Master Mix (High ROX Premixed) (Cat# Q341-02-AA, Vazyme, China) by the LightCycler® 480 System (Roche, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... 50 µL of Kapa Hifi Hotstart Ready Mix (Roche) and 40 µL of Nuclease-free water (Invitrogen) ...
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bioRxiv - Developmental Biology 2024Quote: ... Western blot was stained with the following primary antibodies: mouse anti-GFP antibody (11814460001, Roche) and rabbit anti-HA antibody (H6908 ...
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bioRxiv - Developmental Biology 2024Quote: ... After post-hybridisation washed and blocking with BMB (Roche), embryos were treated with anti-DIG antibody coupled to alkaline phosphatase (Merck ...
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bioRxiv - Developmental Biology 2024Quote: ... The probes were detected with AP coupled with anti-digoxigenin Fab fragments (Roche, 11093274910). AP reaction was developed with 4-Nitro blue tetrazolium chloride (NBT ...
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bioRxiv - Developmental Biology 2024Quote: ... in 1% blocking regent (Roche, 1096176)/PBS ...
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bioRxiv - Developmental Biology 2024Quote: Pitx1 WISH was performed on E12.5 embryos with a digoxigenin-labelled Pitx1 antisense probe designed from a cloned antisense probe (PCR DIG Probe Synthesis Kit, Roche 11636090910). Experimental procedure followed the protocol outlined in (Kragesteen et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were then resuspended in cold lysis buffer (10 mM Tris, pH 7.5, 10 mM NaCl, 5 mM MgCl2, 1 mM EGTA, Protease Inhibitor (Roche, 04693159001)) ...
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bioRxiv - Developmental Biology 2024Quote: ... the samples were incubated overnight with Streptavidin-POD conjugate (Roche, 1:500 dilution) and then washed ...
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bioRxiv - Genetics 2024Quote: ... cut to very small pieces using a scalpel blade and digested using Collagenase D (Roche, Cat No. 11088858001, 1 mg/ml) /DNase I (Thermo Fisher ...
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bioRxiv - Genetics 2024Quote: ... qPCR was performed using a LightCycler® 96 Instrument (Roche) and SsoFast™ EvaGreen® Supermix with Low ROX (Bio RAD #1725211 ...
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bioRxiv - Genetics 2024Quote: ... were run with molecular weight standards Marker VIII (Roche) at 100V for 60 min in 1X TBE ...
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bioRxiv - Developmental Biology 2024Quote: ... and amplified by PCR with HiFi Kapa Hot start ReadyMix (Kapa Biosystems, KK2601). After clean-up with SPRI beads ...
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bioRxiv - Developmental Biology 2024Quote: ... Plasma was stored at −30°C for analysis of leptin and insulin (CrystalChem Inc., Chicago, IL, USA) and triglyceride content (Roche triglyceride reagent ...
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bioRxiv - Developmental Biology 2024Quote: ... 28 using the KAPA Hyperprep Kit (KAPA Biosystems, Cat. #KK8504) with the following modifications ...
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bioRxiv - Developmental Biology 2024Quote: ... The labelling efficiency of probes was tested by performing a dot plot following the protocol provided by Roche. The probes that exhibited strong signals in the dot plot were considered suitable for subsequent fluorescence in-situ hybridization ...
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bioRxiv - Developmental Biology 2024Quote: Antisense digoxygenin-labeled probes (Genius kit; Roche, Indianapolis, IN) were synthesized using template cDNA encoding X ...
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bioRxiv - Developmental Biology 2024Quote: ... The signal is developed in BM Purple (Roche 11442074001) in the dark and the signal checked regularly until the best possible signal-to-noise ratio is achieved ...
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bioRxiv - Developmental Biology 2024Quote: ... and 0.1% sodium dodecyl sulfate) supplemented with 2% v/v protease inhibitor cocktail (Roche; Catalog #11697498001) and 1% v/v phosphatase inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Staining was developed in BM-Purple (Roche #11442074001) for approximately 4 hours at room temperature.
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bioRxiv - Developmental Biology 2024Quote: ... DIG (digoxigenin) RNA labelling mix (Roche, Cat.No. 11277073910). According to the cloning direct of the insert ...
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bioRxiv - Developmental Biology 2024Quote: ... A tablet of protease inhibitor cocktail (Roche) and a tablet of PhosSTOP were dissolved in 4.75ml water ...
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bioRxiv - Developmental Biology 2024Quote: ... and 0.05 mg/ml DNase1 (Roche Diagnostics Gmbh) dissolved in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Developmental Biology 2024Quote: ... TUNEL (In Situ Cell Death Detection Kit, TMR Red, Sigma/Roche); anti-Kmg (Fuller lab).
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bioRxiv - Developmental Biology 2024Quote: ... Per sample 200 ng of total RNA was used for library preparations with the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Kapa Biosystems), including steps of the oligonucleotide hybridization ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 U/µL Protector RNase inhibitor (Roche, 3335399001) in Nuclease-free water ...
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were generated with the RiboErase depletion RNA-sequencing protocol by KAPA (Roche, 8098131702) and sequenced on the Illumina Next Seq 2000 ...
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bioRxiv - Developmental Biology 2024Quote: ... and in situ hybridization probes (Roche DIG RNA Labeling Mix and T7 RNA Polymerase kits) ...
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bioRxiv - Genetics 2024Quote: ... Washed cells were carefully resuspended in 570 μL Buffer A with additives (0.2 mM spermidine, 0.5 mM spermine, 1 mM PMSF, ½ cOmplete ULTRA protease inhibitors tablet, Roche, per 5 mL Buffer A) and permeabilized with 0.1% digitonin in 30 ° C water bath for 5 min ...
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bioRxiv - Genetics 2024Quote: ... and 0.5% NP-40) supplemented with a protease inhibitor cocktail (cOmplete, Mini Protease Inhibitor Cocktail, Roche, Basel, Switzerland) and phosphatase inhibitors (PhosSTOP ...
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bioRxiv - Genetics 2024Quote: ... and protease inhibitors (Complete, EDTA-free, Roche). Lysis was performed with 0.5 mm acid washed beads in a FastPrep instrument (MP ...
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bioRxiv - Genetics 2024Quote: ... The pool was quantified by qPCR with a Kapa Library Quant kit (Kapa Biosystems) and sequenced on an Illumina NovaSeq 6000 (Illumina ...
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bioRxiv - Genetics 2024Quote: ... Strand-specific and dual-barcode indexed RNA-seq libraries were generated from 450 ng total RNA each using the Kapa RNA-seq Hyper kit (Kapa Biosystems-Roche, Basel, Switzerland) and both the QIAseq FastSelect–5S/16S/23S ribodepletion and FastSelect rRNA Plant reagents (Qiagen ...
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bioRxiv - Genetics 2024Quote: ... Strand-specific and dual-barcode indexed RNA-seq libraries were generated from 450 ng total RNA each using the Kapa RNA-seq Hyper kit (Kapa Biosystems-Roche, Basel, Switzerland) and both the QIAseq FastSelect–5S/16S/23S ribodepletion and FastSelect rRNA Plant reagents (Qiagen ...
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bioRxiv - Genetics 2024Quote: ... 0.1% Na-Doc) with freshly added EDTA-free protease inhibitors (Roche), 1mM PMSF and 100 U/ml SuperaseIn (Invitrogen ...
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bioRxiv - Genetics 2024Quote: ... the library was quantified by qPCR with a Kapa Library Quant kit (Kapa Biosystems/Roche, Basel, Switzerland). The library was sequenced on one flow cell of Aviti sequencer (Element Biosciences ...
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bioRxiv - Developmental Biology 2024Quote: ... ovaries were crosslinked in 250 µL YSS buffer (50 mM pH 8.0 Tris HCl, 50 mM NaCl, 0.1% NP40, 1 U/mL of RNAsin (Roche, 3335402001), protease inhibitor (Thermo Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... heads were rinsed for 5 minutes in PBST five times and incubated in Blocking Reagent (Roche, 1:5000 dilution) for 60 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... Digoxygenin or biotin-labelled probes were synthesized using the labelling kit (Roche). The template DNA for the Osi10b RNA antisense probe was amplified with the primer set (Forward ...
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bioRxiv - Genetics 2024Quote: ... the library was quantified by qPCR with a Kapa Library Quant kit (Kapa Biosystems/Roche, Basel, Switzerland). The library was sequenced on one flow cell of Aviti sequencer (Element Biosciences ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Poly A+ RNA from total RNA was extracted using the mRNA isolation kit (Roche). Remaining genomic DNA in isolated mRNA were digested with Turbo DNase (Thermo Fisher) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The four paired-end libraries were quantified by qPCR using the KAPA Library Quantification Kit (Roche). Genome sequencing was conducted on the Illumina MiSeq platform at the Federal University of Rio Grande do Sul ...
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bioRxiv - Developmental Biology 2024Quote: ... Fasting blood glucose was measured at the end of treatment after a 12-hr fast using a single tail nick bleed to measure blood glucose levels via glucometer (Cat# 68623221868, Accu-Chek Performa, Roche). Perigonadal white adipose tissue (PWAT ...
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bioRxiv - Developmental Biology 2024Quote: ... then hybridized overnight at 65 °C with digoxigenin-labeled RNA probes (Roche, 11277073910). The probes were detected with AP coupled with anti-digoxigenin Fab fragments (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... AP reaction was developed with 4-Nitro blue tetrazolium chloride (NBT, Roche, 11383213001) and 5-bromo-4-chloro-3’-indolyphosphate p-toluidine salt (BCIP ...
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bioRxiv - Developmental Biology 2024Quote: ... Hematoxylin and eosin staining was performed using a Ventana Discovery Ultra automated slide preparation system (Roche).