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Citations for Roche :
1551 - 1600 of 3852 citations for hsa mir 635 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... libraries were PCR amplified with HiFi Polymerase (Kapa Biosystems) for 12 cycles ...
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bioRxiv - Plant Biology 2024Quote: ... and a LightCycler 480 Real-Time PCR instrument (Roche). cDNA amplification was performed as follows ...
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bioRxiv - Immunology 2024Quote: ... PCR reactions were performed with HiFi Hotstart (KAPA Biosystems). Splint-mediated ligations were carried out with Ampligase Thermostable DNA Ligase (Epicenter Biotechnologies) ...
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bioRxiv - Physiology 2024Quote: ... assessed by Q-PCR KAPA Library Quantification kit (Roche) and with a run test using the iSeq100 (Illumina ...
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bioRxiv - Neuroscience 2024Quote: ... Real-time PCR was performed in LightCycler 480 (Roche Molecular Biochemical ...
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bioRxiv - Plant Biology 2021Quote: ... corresponding stem-loop primers were added to the RT reaction (adapted from Yang et al., 2014) and miR823 levels were measured using Fast SYBR Green Master Mix (Roche) with miRNA823 specific forward primer and a stem-loop specific universal reverse primer ...
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bioRxiv - Microbiology 2019Quote: ... To determine the viral load by RT-qPCR RNA was extracted from apical washes (High Pure Viral RNA kit, Roche). cDNA was prepared using 10 µL RNA (High Capacity cDNA Reverse Transcription kit ...
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bioRxiv - Neuroscience 2021Quote: ... RT-qPCR reactions were carried out using LightCycler® 480 SYBR Green I Master Mix (Roche #04-887-352-001) on a LightCycler® Real-Time PCR System (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... A total of 50 ng DNase-treated RNA was used as template for reverse transcription using either RT Transcriptor from Roche and random hexamers or Moloney Murine Leukemia Virus-RT (MMULV-RT ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was eluted from the beads by incubation for 1h at RT (while rotating) with 150-250 µl wash buffer supplemented with phosphatase inhibitors (PhosSTOP, Roche) and 0.5 mg/ml 3xFLAG peptide (APExBIO) ...
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bioRxiv - Microbiology 2019Quote: ... After washing with 1xPBS containing 0.1% v/v Tween-20, PIP–strips were incubated (1h, RT, agitated) with a monoclonal anti-HA antibody (clone 3F10, monoclonal antibody from Roche) at a dilution of 1:500 in blocking buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... The RNA concentration was measured by a spectrophotometer (NanDrop1000; Thermo) followed by a reverse transcription process using PrimeScript RT reagent kit (Roche). Real time PCR analysis was performed using SYBR green mix (Roche ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR experiments were done with SYBR Green I in a Light Cycler 96® (Roche Applied Science, Mannheim, Germany). For each primer pair (Table 4 ...
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bioRxiv - Neuroscience 2021Quote: ... diluted in 1x maleic acid for 1h at RT and riboprobes were detected by incubating in anti-digoxigenin-AP antibody (1:2000 in 1% sheep serum, Roche) overnight at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... Real-time RT-qPCR reactions were run in a 384-well LightCycler®480 II System (Roche Diagnostics, Mannheim, Germany) using the GoTaq qPCR Mastermix (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNAse was inactivated with 1 µl of Stop Solution at 65°C for 10 min and RT was performed using Transcriptor First Strand cDNA Synthesis Kit (Ref. 04 897 030 001, Roche) in a final volume of 20 µl ...
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bioRxiv - Cell Biology 2021Quote: ... for 1h at RT and incubated with a primary antibody diluted in blocking solution (sheep anti-digoxigenin, 1/200, Roche) overnight at +4°C in a dark humidified chamber ...
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bioRxiv - Microbiology 2020Quote: ... Library concentrations for pooling of barcoded samples was assessed by RT-qPCR with a KAPA Library Quantification kit (KAPA Biosystems) as recommended for high sensitivity ...
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bioRxiv - Cell Biology 2020Quote: ... TNbuffer 10 min at RT and TNT buffer 15’ at RT) and by the incubation of cryo-section using anti-digoxigenin-ap fab fragments (1/100, #11093274910, Roche) dissolved in TNbuffer for 2 hours at RT ...
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bioRxiv - Developmental Biology 2022Quote: ... The mRNA was used in an RT reaction that was specifically selected for the barcoded mRNA (Transcriptor High Fidelity Roche). The RT product was PCR amplified and size selected using Agencourt AMPure beads (Beckman Coulter) ...
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bioRxiv - Genomics 2019Quote: Validation of the RNA-Seq experiment was completed with the Universal Probe Library (UPL) RT-qPCR system (Roche Diagnostics ©). RNA samples analyzed were those used for RNAseq analyses (aphid collections from location 1 ...
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bioRxiv - Microbiology 2019Quote: ... RT-qPCR analysis of cytokine related porcine gene expression was performed using the KAPA SYBR-FAST qPCR Kit (KAPA Biosystems), using the primers shown in Table 1 ...
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bioRxiv - Developmental Biology 2020Quote: ... They were then blocked in PBS with 3% BSA for 3h at RT and incubated overnight at 4°C with a peroxidase-labeled anti-DIG antibody (Roche) diluted 1:1500 in PBS with 3% BSA ...
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bioRxiv - Biochemistry 2020Quote: RT activity was measured using an ELISA-based colorimetric reverse transcriptase activity assay (Catalog No. 11468120910, Roche Diagnostics, Indianapolis, IN). The HEPES integration buffer was used for the reaction ...
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bioRxiv - Cell Biology 2021Quote: ... A volume corresponding to 25-50 ng of cDNA was used for each RT-qPCR reaction using Roche LightCycler 480 SYBR Green I Master (04707516001, Roche) sequence detection system ...
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bioRxiv - Immunology 2020Quote: ... RT-qPCR was performed using the LightCycler 480 Probe Master Kit and the LightCycler 480 II system (Roche Applied Science) as described previously92 ...
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bioRxiv - Microbiology 2021Quote: ... The samples were subsequently diluted by adding 150 μL of water and 4 μL was used for RT-qPCR performed on a LightCycler96 platform (Roche) using the iTaq Universal Probes One-Step RT-qPCR kit (BioRad ...
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bioRxiv - Microbiology 2021Quote: ... Library concentration for pooling of barcoded samples was assessed by RT-qPCR with a KAPA Library Quantification kit (KAPA Biosystems) as recommended for high sensitivity ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were pelleted by centrifugation (750 x g, 2 min, RT) and washed in 4 ml vPBS containing EDTA-free protease inhibitor (Roche). The washed cells were again pelleted by centrifugation (750 x g ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was then washed and incubated 1 hs at RT with goat anti-mouse secondary antibody conjugated to HRP (Roche) at 1/20000 dilution in PBST - 5% milk.
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bioRxiv - Physiology 2023Quote: ... Each RT-qPCR assay was deposited in triplicates on a FrameStar® 384-well skirted qPCR plate (Roche Diagnostics, Switzerland). The qPCR program was initiated at 95°C for 10 minutes to denature the cDNA and activate the TAQ polymerase enzyme in a thermocycler (Lightcycler® 480 II Roche thermocycler ...
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bioRxiv - Microbiology 2020Quote: ... the DNA was ligated to unique i5/i7 barcoded primer combinations using the Illumina Nextera XT Index Kit v2 and KAPA HiFi HotStart ReadyMix (2X; KAPA Biosystems) and purified using AmPure Beads XP (Agencourt) ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... Coralville, Iowa, USA) and 0.25 µl reverse primer (5 µM, IDT) and was analyzed on a LC480 instrument (Roche, Basel, Switzerland).
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bioRxiv - Evolutionary Biology 2020Quote: ... Amplifications were performed using 15 cycles with Nextera-style dual indexing primers (Kircher et al., 2011) and KAPA HiFi Hotstart ReadyMix (Kapa Biosystems). Library concentrations were measured with Qubit dsDNA High Sensitivity Assay kit.
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bioRxiv - Molecular Biology 2021Quote: ... A two-hundredth aliquot of the cDNA was subjected to qRT-PCR with primers (final concentration 200 nM each) and MGB probe (final concentration 100 nM; the Universal Probe Library [UPL], Roche Diagnostics) sets as shown in Supplementary File 4 for human ACE2 ...
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bioRxiv - Immunology 2020Quote: ... Tagmented DNA was ligated to unique i5/i7 barcoded primer combinations using the Illumina Nextera XT Index Kit v2 and KAPA HiFi HotStart ReadyMix (2X; KAPA Biosystems) and then purified using AmPure Beads XP (Agencourt) ...
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bioRxiv - Immunology 2021Quote: ... Tagmented DNA was ligated to a unique i5/i7 barcoded primer combinations using the Illumina Nextera XT Index Kit v2 and KAPA HiFi HotStart ReadyMix (Roche, #07958927001), and then purified using AmPure Beads XP (Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... the DNA was ligated to i5/i7 barcoded primers using the Illumina Nextera XT Index Kit v2 and KAPA HiFi HotStart ReadyMix (2X; KAPA Biosystems). Next the DNA was purified using AmPure Beads XP (Agencourt) ...
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bioRxiv - Cancer Biology 2020Quote: ... Il1r1/IL1R1 and Gapdh/GAPDH qPCR was performed using specific primers and Lightcycler 480 Sybr Green I Master (Roche; Mannheim, Germany) in a Lightcycler 480 II (Roche Diagnostics) ...
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bioRxiv - Plant Biology 2021Quote: ... WTK4 transcript accumulation was confirmed by PCR amplification using the primers JS693xJS671 followed by a semi-nested reaction with primers JS696xJS671 using KAPA Hifi HotStart Polymerase (KK2502, Kapa Biosystems, Hoffmann-La Roche) with an annealing temperature of 60°C and extension time of 3 min ...
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bioRxiv - Microbiology 2020Quote: ... The PCR was performed in 10 μl volume reactions with 0.2 μM primer concentration and using the Kapa HiFi HotStart Ready Mix (Roche, ref. KK2602). Cycling conditions were initial denaturation of 3 min at 95 °C followed by 20 cycles of 95 °C for 30 s ...
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bioRxiv - Microbiology 2019Quote: ... and pooled for Illumina MiSeq sequencing using custom sequencing primers and the MiSeq Reagent v2 500 cycle Kit (Roche, Branford, CT) following the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2020Quote: ... and the final concentration was estimated by qPCR using primers shown in Supplementary Table S5 and KAPA Library Quantification Kit Illumina Platforms KK4828 (Roche #07960166001) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.5 μL of each forward and reverse primers (5 pM) and 5 μL of 2X FastStart SYBR Green master mix (# 6924204001, Roche, USA). The PCR cycling condition set in Light Cycler 480 instrument (Roche Diagnostics ...
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bioRxiv - Pathology 2022Quote: ... labeling probe (359 bp) was synthesized using primers TiPV-Fq and TiPV-NS1-R (Table 1) and DIG-labeling mix reagent (Roche, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... Libraries are validated on a Bioanalyzer DNA High Sensitivity chip to check for size and absence of primer dimers and quantified by qPCR using Kapa Library Quantification Illumina/ABI Prism Kit protocol (KAPA Biosystems). Validated libraries were paired-end sequenced on an Illumina HiSeq X platform following Illumina’s recommended protocol to generate paired-end reads of 150-bases in length.
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bioRxiv - Cancer Biology 2022Quote: ... using the gRNA_enrichment1_fw (5’-GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTCTTGTG-GAAAGGACGAAACACCG-3’) and gRNA_enrichment1_rv (5’-CTACACGAC-GCTCTTCCGATCT-3’) primers and the 2x KAPA HiFi HotStart Ready Mix (Roche, Cat. No. KK2601). In a subsequent PCR ...
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bioRxiv - Cancer Biology 2022Quote: ... Human samples were analyzed using a combination of gene-specific primers and Universal Probe Library (UPL) hydrolysis probes (Roche Life Science). Threshold cycle (Ct ...
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bioRxiv - Plant Biology 2019Quote: ... was performed using gene-specific primers (Supplementary Table 1) and the fluorescent intercalating dye Kapa SYBR Green (Kapa SYBR FAST qPCR Master Mix Universal, Kapa Biosystems) in a BIORAD CFX ConnectTM Real-Time PCR Detection System ...
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bioRxiv - Clinical Trials 2019Quote: ... Tagmented DNA was ligated to unique i5/i7 barcoded primer combinations using the Illumina Nextera XT Index Kit v2 and KAPA HiFi HotStart ReadyMix (2X) (KAPA Biosystems), and then purified using AmPure Beads XP (Agencourt) ...