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Citations for Roche :
1801 - 1850 of 3852 citations for hsa mir 635 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: The amount of pmoA DNA and mRNA copies in lake water during overturn was quantified by qPCR using the 189f-mb661 primer pair31 on a LightCycler 480 (Roche Diagnostics, Rotkreuz, Switzerland). 10µl reactions were performed with 0.2 µM primer ...
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bioRxiv - Genomics 2020Quote: ... Libraries were amplified in 50 μl reactions containing 150 pmol of P1.1 and P3 primer and Kapa HiFi HotStart Library Amplification kit (Cat# kk2612, Roche Sequencing and Life Science). The amplification was incubated at 95°C for 45 seconds ...
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bioRxiv - Immunology 2021Quote: ... 72°C for 1 min) including the TruSeq primer mix and the KAPA Hifi Uracil+ Poly-merase Master Mix (Kapa Biosystems, Cat. #KK2801). The PCR product was purified and size controlled by Agilent Technologies 2100 Bioanalyzer (High Sensitivity DNA Chip) ...
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bioRxiv - Neuroscience 2021Quote: ... For each gene the specific forward and reverse primers and probe were combined with Fast Start Universal Probe Master (Rox) Mastermix (Roche Diagnostics GmbH, Germany) and RNase free H2O (Sigma ...
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Tryptophan Metabolites And Their Predicted Microbial Sources In Fecal Samples Of Healthy IndividualsbioRxiv - Microbiology 2024Quote: ... Libraries were amplified for 13 PCR cycles in 50 μl reactions containing 150 pmol of P1.1 (5’-AATGATACGGCGACCACCGAGA) and P3 (5’-CAAGCAGAAGACGGCATACGAGA) primer and Kapa HiFi HotStart Library Amplification kit (Cat# kk2612, Roche Sequencing and Life Science). Library quantification and size estimation were performed using a Fragment Analyzer (Agilent Technologies ...
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bioRxiv - Plant Biology 2023Quote: ... and purified based on the manufacturer’s instructions before sample barcoding with xGen Stubby Adapter and Unique Dual Index Primers (IDT) and library preparation using KAPA HyperPrep Kit (Kapa Biosystems-Roche, Wilmington, MA). The library was then sequenced by Illumina MiSeq nano PE150 at the Georgia Genomics and Bioinformatics Core ...
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bioRxiv - Genetics 2024Quote: ... One microliter of each primer pool was combined with 50 ng of sample plasmid DNA and 25 µL Kapa Hifi Hotstart ReadyMix (Kapa Biosystems Cat# KK2602), and topped off to a total volume of 50 µL with water ...
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bioRxiv - Biochemistry 2021Quote: Relative mRNA expression was determined by RT-qPCR using LightCycler® 480 SYBR Green I Master Mix on a LightCycler® 480 instrument (Roche). All reactions were performed in 10 or 20 µL volumes ...
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bioRxiv - Plant Biology 2021Quote: ... and quantified by RT-qPCR in a Bio-Rad CFX384 Touch detection system using the KAPPA KK4824 kit (Kapa Biosystems, Wilmington, USA). Two 150-bp single-end sequencing libraries were prepared using the NextSeq 500/550 High Output Kit (Illumina ...
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bioRxiv - Plant Biology 2020Quote: ... RT–qPCR was performed in a 20 μl reaction with the Fast Start Universe SYBR Green Master (ROX) mix (Roche, IN, USA). The expression levels were normalized to glyceraldehyde-3-phosphate-dehydrogenase (GADPH) ...
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bioRxiv - Cancer Biology 2020Quote: ... were prepared in a final volume of 20 μL and then subjected to RT-qPCR using LightCycler® 480 instrument (Roche Diagnostics). qPCR reaction was performed under the following conditions ...
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bioRxiv - Neuroscience 2020Quote: ... sections were blocked with 10% lamb serum in TBST for 1 hour at RT and incubated with alkaline phosphatase conjugated to DIG antibody (Roche, Basel, Switzerland) in TBST ...
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bioRxiv - Plant Biology 2021Quote: Gene expression analysis via RT-qPCR was performed using a LightCycler480 instrument and LightCycler480 SYBR Green Master Kit reagents (Roche, Indiana, IN). Relative gene expression levels were determined using a standard curve method ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was diluted to a final concentration of 5 ng/μl and RT–qPCR was performed with KAPA SYBR® FAST qPCR kits (KAPA Biosystems) on a C1000 thermal cycler ...
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bioRxiv - Plant Biology 2021Quote: ... TaNADH-GoGAT (Li et al., 2020) and TaAMT1c (Li et al., 2017) was performed by RT-qPCR using a Roche LightCycler® 96 System (Roche, UK) and a LightCycler® RNA Master SYBR Green I one-step kit (Roche ...
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Glucocorticoid receptor collaborates with pioneer factors and AP-1 to execute genome-wide regulationbioRxiv - Genomics 2021Quote: ... followed by addition of 100 μL ChIP Elution Buffer (50 mM Tris-HCl, pH 7.5 @ RT, 10 mM EDTA, 1% SDS) and 20 μL Pronase (Roche, 20 mg/mL) for incubation at 42°C for 2 hours ...
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bioRxiv - Pathology 2022Quote: RNA was reverse-transcribed and amplified using the TaqMan Fast Virus 1-Step Master Mix RT-qPCR kit (LifeTechnologies, Carlsbad, CA) on the LightCycler 480 or LC96 instrument (Roche, Indianapolis, IN), and quantified by interpolation onto a standard curve made up of serial tenfold dilutions of in vitro transcribed RNA ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative PCR (qPCR) was performed using TaqMan Probe system (Roche Diagnostics) on the Applied Biosystems 7500 (Applied Biosystems ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 25μl of 1X PCR buffer B (Kapa Biosystems, Boston, MA, USA) pre-warmed at 65°C was added then the legs were ground ...
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bioRxiv - Immunology 2021Quote: ... Quantitative real time PCR was performed on Light cycler 480 (Roche) instrument with Kapa Sybr Fast Universal qPCR master mix (Cat ...
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bioRxiv - Microbiology 2019Quote: ... and the Light Cycler 480 real-time PCR system (Roche, Switzerland); cycling conditions were as follows ...
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CRISPR-SID: identifying EZH2 as a druggable target for desmoid tumors via in vivo dependency mappingbioRxiv - Cancer Biology 2021Quote: ... combined with a LightCycler® 480 Real-Time PCR System (Roche). Expression was normalized to housekeeping genes Actb ...
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bioRxiv - Pathology 2022Quote: ... PCRs were carried out in a LightCycler 480 system (Roche Diagnostics) with initial denaturation for 3 min at 95°C and then 39 cycles of 10 s at 95°C and 30 s at 55°C ...
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bioRxiv - Genetics 2022Quote: ... The CAN1 gene was amplified by PCR using KAPA HiFi (Roche) in 6 overlapping fragments that were purified using the Zymo ZR-96 DNA Clean-up Kit ...
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bioRxiv - Developmental Biology 2022Quote: ... The barcode sequencing libraries were quantified by quantitative PCR (KAPA Biosystems Library Quantification Kit for Illumina platforms P/N KK4824) ...
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bioRxiv - Neuroscience 2020Quote: ... qRT-PCR was performed with a Light cycler 480 (Roche, Switzerland).
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bioRxiv - Molecular Biology 2021Quote: ... The quantitative PCR was performed on a Light Cycler 480 (Roche) using 2 μl of the diluted cDNAs (1:5 ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative PCR was performed using SYBR Green I Master Mix (Roche) and the LightCycler 480 (Roche) ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR reactions were performed with FastStart Universal SYBR Green Master (Roche) in a Bio-Rad IQ5 system ...
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bioRxiv - Immunology 2021Quote: ... The PCR reaction was performed with KAPA HiFi HotStart ReadyMix (Roche) at 95°C for 3 min ...
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bioRxiv - Cell Biology 2019Quote: ... Real time quantitative PCR was performed using SYBR Green (Kapa Biosystems) or TAQMAN probes (details provided in supplementary table 1 ...
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bioRxiv - Cell Biology 2019Quote: ... The final sequencing libraries were assayed with quantitative PCR (KAPA Biosystems Quantification Kit for Illumina Libraries) ...
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bioRxiv - Developmental Biology 2019Quote: ... Genomic PCR was carried out using KAPA HiFi HotStart (KAPA Biosystems) on a Veriti 96-well Thermal Cycler (Applied Biosystems ...
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bioRxiv - Developmental Biology 2020Quote: ... Real time PCR analysis was performed using SYBR green mix (Roche) and the values were normalized to β-actin values ...
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bioRxiv - Microbiology 2019Quote: ... 10 µL of 2x KAPA HiFi HotStart PCR mix (Roche, Switzerland), and PCR grade water to final volume ...
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bioRxiv - Plant Biology 2019Quote: ... Quantitative PCR (qPCR) reactions were run on a LightCycler 480 (Roche), using 2.5 μL of LightCycler 480 SYBR Green I Master mix and 2.5 μM forward and reverse primers in a final volume of 5 µL ...
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bioRxiv - Bioengineering 2019Quote: ... qRT-PCR was performed on a LightCycler® 480 (Roche, Switzerland) with the LightCycler® 480 Probes Master (04887301001 ...
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bioRxiv - Cancer Biology 2019Quote: ... was detected using Power SYBR Green PCR master mix (Roche, Switzerland). Data were collected and analyzed quantitatively on ABI Prism 7500 sequence detection system (Life Technologies Corporation ...
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bioRxiv - Molecular Biology 2019Quote: ... qRT-PCR was performed on LightCycler 96 instrument (Roche, Basel, Swizerland) using THUNDERBIRD SYBR qPCR Mix (TOYOBO Co ...
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bioRxiv - Microbiology 2019Quote: ... DNA was purified using High Pure PCR Product Purification Kit (Roche), according to the recommendations of the manufacturer ...
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bioRxiv - Cell Biology 2019Quote: ... in a LightCycler 480 Real-Time PCR System (Roche Applied Science) as follows ...
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bioRxiv - Neuroscience 2021Quote: ... Quantitative PCR (qPCR) was performed on the Light Cycler LC480 (Roche) using the TB Green Ex Taq II polymerase (Takara RR820L) ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR amplicons were generated using Kapa HotStart Readymix (Kapa Biosystems KK2602) supplemented with 1 M Betaine (Sigma B0300-5VL ...
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bioRxiv - Microbiology 2021Quote: ... and the LightCycler 480 Real-Time PCR System (Roche, Basel, Switzerland). RNA extraction was performed using the Roche High Pure Viral RNA Kit (Roche ...
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bioRxiv - Bioengineering 2022Quote: ... Semi-quantitative PCR was performed using a LightCycler 480 (Roche Diagnostics) and the LightCycler DNA green master mix (Roche Molecular Systems ...
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bioRxiv - Immunology 2021Quote: ... All PCRs were performed using Kapa HiFi Hotstart polymerase (Kapa Biosystems). Amplicons were separated on a 2% agarose gel ...
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bioRxiv - Biochemistry 2020Quote: ... then purified with a High Pure PCR Product Purification Kit (Roche). Following this ...
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bioRxiv - Cancer Biology 2020Quote: PCR was performed by using the KAPA2G FAST mix (Kapa Biosystems) according to the manufacturer’s recommendations and by using the corresponding oligos in supplementary Table 1.
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bioRxiv - Cancer Biology 2020Quote: ... Quantitative PCR was performed in a LightCycler 480 (Roche, Mannheim, Germany) using the corresponding LightCycler 480 Probes Master and the pre-designed Probes of the Universal ProbeLibrary (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative PCR was performed using SYBR fast qPCR mastermix (KAPA Biosystems) on the Roche 480 Lightcycler ...