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Citations for Roche :
1651 - 1700 of 3852 citations for hsa mir 635 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... Blots were blocked for at least 1 hour in blocking solution at RT (5% milk in 1xTBS) before probing with primary antibody in blocking solution (α-HA-HRP, 1:2500 (Roche); α-PGB1 ...
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bioRxiv - Genetics 2019Quote: ... which was diluted 1:10 to be used in RT-qPCR carried out using LightCycler 480 SYBR Green I Master (Roche, 4707516001) under the following conditions ...
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bioRxiv - Biochemistry 2020Quote: ... 150 mM NaCl and 1 mM DTT and incubated at RT in 0.05 mg/mL sequencing grade trypsin (Roche Applied Science). Digestions were stopped with 2X Laemmli sample buffer and placed on ice until analysis ...
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bioRxiv - Cancer Biology 2022Quote: Gene expression knockdown in response to shRNA expression was verified by RT-QPCR as previously described (Saurty-Seerunghen et al 2019) using the LightCycler480 (Roche, France) and the SYBR Green PCR Core Reagents kit (Bimake.com) ...
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bioRxiv - Cancer Biology 2023Quote: ... Then embryos were incubated overnight at RT in AP-conjugated anti-DIG antibody (Roche, 1:1000 diluted in the blocking buffer). On the third day ...
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bioRxiv - Immunology 2023Quote: ... DNA was incubated for 10 minutes at 65°C before performing RT qPCR using the KAPA SYBR FAST qPCR Kit from KAPA Biosystems. Primers used for real-time qPCR can be found in Supplementary Table 1.
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bioRxiv - Microbiology 2019Quote: ... PCR targeting the V4 region of archaeal 16S rRNA genes was conducted with KAPA HiFi HotStart PCR kit (KAPA Biosystems, Cape Town, South Africa) and barcoded primer sets Arc519F/806R (Table S1) ...
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bioRxiv - Developmental Biology 2020Quote: ... Quantitative reverse transcription-PCR (qRT-PCR) assays were performed using an ABI Prism 7900 sequence-detection system using SYBR (Roche Applied Science, Indianapolis, IN). Relative mRNA expression was calculated by the ΔCt method and the values were normalized to the expression of ARBP ...
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bioRxiv - Genetics 2019Quote: ... The PCR reaction mixture in a 20 μl volume containing 10 μl of 2×SYBR Green PCR Master Mix (Roche, Mannheim, Germany, code#06402712001), 2 μl diluted reverse transcriptase product (1:100) ...
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bioRxiv - Immunology 2019Quote: ... Capture bead-bound sample pools were washed and amplified for 16 PCR cycles using KAPA HiFi Hostart ReadyMix and LM-PCR oligos (Roche Diagnostics Corp.,Indianapolis, IN). Final products were visualized on a high-sensitivity DNA Advanced Analytical Fragment Analyzer assay (Advanced Analytical Technologies Inc. ...
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bioRxiv - Immunology 2019Quote: ... after which bisulfite-converted libraries were amplified with 12 polymerase chain reaction (PCR) cycles using KAPA HiFi Hotstart Uracil+ ReadyMix and Ligation-Mediated PCR oligos (Roche Diagnostics Corp.,Indianapolis, IN). Samples submitted with less than one microgram of extracted gDNA were amplified with an additional 2 PCR cycles to increase concentration pre-capture ...
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bioRxiv - Molecular Biology 2024Quote: ... Quantitative real-time PCR (qRT-PCR) was performed using MonAmp™ ChemoHS qPCR Mix (Monad, Suzhou, China) and LightCycler® 480 II (Roche Diagnostics, Germany). Table S2 contains the primer sequences ...
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bioRxiv - Microbiology 2020Quote: ... and final elution in 40 µL PCR-grade water (Roche). Extraction blanks were processed alongside to assess laboratory contamination ...
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bioRxiv - Cell Biology 2020Quote: ... qRT-PCRs were performed using SYBR green technology (KAPA Biosystems). Data were analyzed by comparative threshold cycle (delta delta Ct ...
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bioRxiv - Cell Biology 2020Quote: ... and real-time PCR was performed (LightCycler96, Roche, Basel, CHE) with SYBR Green (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... The sequencing libraries were quantified by quantitative PCR (KAPA Biosystems Library Quantification Kit for Illumina platforms P/N KK4824 ...
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bioRxiv - Immunology 2021Quote: ... therefore a PCR amplification and detection instrument LightCycler 480 (Roche) was used ...
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bioRxiv - Immunology 2021Quote: ... PCR reactions were performed using KAPA HiFi polymerase (KAPA biosystems) using the following primer pairs ...
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bioRxiv - Evolutionary Biology 2021Quote: ... using Roche High Pure™ PCR Template Preparation Kits (Roche Molecular Systems ...
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bioRxiv - Neuroscience 2022Quote: ... SYBR green based PCR was performed with SYBR mix (Roche). The qPCR amplification was performed using the LightCycler 480 System (Roche) ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was PCR amplified with Kapa Hifi DNA polymerase (Roche), quantified using picogreen dye (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Real time PCR was performed by Light Cycler 96 (Roche Health Care Thermalcycler 96 ...
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bioRxiv - Molecular Biology 2020Quote: ... 12.5 μL 2x KAPA HiFi HotStart PCR mix (Kapa Biosystems), and 1 μL of 25 μM PE1/PE2 index primer mix ...
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bioRxiv - Microbiology 2019Quote: ... with the KAPA HiFi HotStart ReadyMix PCR Kit (KAPA biosystems), in a volume of 25 µl ...
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bioRxiv - Microbiology 2019Quote: ... 25ng of cDNA and PCR grade water (Roche, Hertfordshire, UK). An initial denaturation cycle of 95°C for 10min was used followed by 45 cycles of 10s at 95°C ...
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bioRxiv - Developmental Biology 2019Quote: ... qRT-PCR was performed on a LightCycler 96 Instrument (Roche) using gene-specific and control elF4A primes (Table S5) ...
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bioRxiv - Plant Biology 2019Quote: ... Quantitative PCR was performed in the LightCycler 480 system (Roche) using primers listed in Table S1 ...
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bioRxiv - Plant Biology 2019Quote: ... in a Light Cycler 96 Real-Time PCR instrument (Roche). For semi-quantitative RT-PCR assays ...
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bioRxiv - Microbiology 2019Quote: ... and hybridized with PCR-generated digoxigenin-labeled probes (Roche Diagnostics) using DIG Easy Hyb hybridization solution (Roche Diagnostics ...
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bioRxiv - Biochemistry 2019Quote: ... Expanded high fidelity PCR system (Roche Life Science, Indianapolis, IN) was used to amplify the full length PAR2 cDNA coding region ...
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bioRxiv - Genomics 2019Quote: ... or Roche High Pure™ PCR Template Preparation Kits (Roche Molecular Systems ...
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bioRxiv - Genomics 2019Quote: ... in a Light Cycler 96 Real Time PCR system (Roche). Briefly ...
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bioRxiv - Cell Biology 2019Quote: ... Quantitative PCRs were performed on the LightCycler 480 System (Roche) using the KAPA SYBR FAST qPCR Kit (KAPA Biosystems ...
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bioRxiv - Pathology 2021Quote: ... qRT-PCR were performed on a LightCycler 480 instrument (Roche) using QuantiTect SYBR Green RT-PCR Kit and QuantiTect Primers (Qiagen) ...
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bioRxiv - Genetics 2020Quote: ... PCR reactions were performed using SYBR Green Master Mix (Roche). PCR conditions were as follows ...
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bioRxiv - Cancer Biology 2020Quote: ... all PCRs were performed using HotStart ReadyMix (Kapa Biosystems #KK2601). Restriction enzymes ...
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bioRxiv - Genomics 2020Quote: ... or Roche High Pure™ PCR Template Preparation Kits (Roche Molecular Systems ...
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bioRxiv - Plant Biology 2020Quote: ... amplified by PCR with KAPA HiFi HS ReadyMix (KAPA BIOSYSTEMS) and size-selected with the E-Gel size select (Life Technologies ...
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bioRxiv - Plant Biology 2020Quote: ... the LightCycler 480 Real-Time SYBR Green PCR System (Roche) was used ...
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bioRxiv - Cancer Biology 2021Quote: ... qRT-PCR was carried out using SYBR Green (Roche, Germany), and β-actin was used as control ...
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bioRxiv - Cell Biology 2021Quote: ... and a LightCycler® II real-time PCR system (Roche). Dissociation curve analysis of the amplified DNA melting temperature showed that each primer set gave a single and specific product (Sun et al. ...
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bioRxiv - Plant Biology 2020Quote: ... with KAPA3G Plant PCR Kit (Kapa Biosystems, Wilmington, MA, USA), following Section 2 ...
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bioRxiv - Microbiology 2020Quote: ... and final elution in 40 μL PCR-grade water (Roche). Extraction blanks were processed alongside to assess laboratory contamination ...
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bioRxiv - Microbiology 2021Quote: ... or plasmid inserts were amplified with KAPA PCR (Kapa Biosystems) using full length TruSeq Illumina primers ...
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bioRxiv - Neuroscience 2021Quote: ... PCR amplification using KAPA2G Fast HotStart Genotyping Mix (Kapa Biosystems) with flanking oligos (S4 Table ...
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bioRxiv - Microbiology 2021Quote: ... with the KAPA HiFi HotStart ReadyMix PCR Kit (KAPA Biosystems), in a volume of 25 μl ...
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bioRxiv - Plant Biology 2020Quote: ... on a Lightcycler 480 Real-Time PCR system (Roche Diagnostics). Relative quantification of expression was calculated after data analysis by the Lightcycler 480 software (Roche Diagnostics) ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative PCR was performed using FAST SYBR green mix (Roche) on the ABI 7500 real-time PCR System (Applied Biosystems) ...
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bioRxiv - Cell Biology 2020Quote: ... qRT-PCR was performed using a LightCycler® 480 (Roche) and TATAA SYBR® GrandMaster® Mix (TATAA Biocenter ...
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bioRxiv - Bioengineering 2022Quote: ... PCR reactions were performed using KAPA HiFi HotStart ReadyMix (Roche) according to the manufacturer’s instructions ...