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Citations for Roche :
1401 - 1450 of 3664 citations for hsa mir 635 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... DNA fragments were amplified using the Kapa HiFi Hotstart Uracil+ PCR mix in 14 cycles of PCR (Kapa Biosystems, Wilmington, MA, USA). Bisulfite libraries were sequenced on an Illumina HiSeq3000 instrument (150 bp paired-end reads).
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Gut microbial disruption in critically ill patients with COVID-19 associated pulmonary aspergillosisbioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 infection was confirmed by quantitative reverse transcription PCR (TaqMan™-PCR performed on Roche cobas® 6800, Basel, Switzerland), performed on nasopharyngeal swabs or tracheal fluid ...
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bioRxiv - Microbiology 2022Quote: ... then guide sequences were amplified from gDNA and the plasmid pool by nested PCR using KAPA HIFI Hotstart PCR kit (Kapa Biosystems, KK2501), as previously described in (Young et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... The relative expression levels were determined by performing a quantitative reverse transcription PCR (qRT-PCR) analysis using a LightCycler 480 system (Roche, Basel, Sweitzer) with an Evo M-MLV RT-PCR kit (Accurate Biotechnology Co. ...
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bioRxiv - Molecular Biology 2023Quote: ... the cDNA inserts of the plasmids were isolated and amplified by low-cycle (12x) PCR using a Kapa2G Robust Hotstart PCR kit (Roche, Ref #07961073001) according to specifications ...
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bioRxiv - Plant Biology 2024Quote: ... Templates were amplified in KOD SYBR qRT-PCR Mix (TOYOBO) using a Light Cycler Nano Real-time PCR Detection System (Roche Applied Science). The PCR was performed according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... corresponding stem-loop primers were added to the RT reaction (adapted from Yang et al., 2014) and miR823 levels were measured using Fast SYBR Green Master Mix (Roche) with miRNA823 specific forward primer and a stem-loop specific universal reverse primer ...
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bioRxiv - Microbiology 2019Quote: ... To determine the viral load by RT-qPCR RNA was extracted from apical washes (High Pure Viral RNA kit, Roche). cDNA was prepared using 10 µL RNA (High Capacity cDNA Reverse Transcription kit ...
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bioRxiv - Neuroscience 2021Quote: ... RT-qPCR reactions were carried out using LightCycler® 480 SYBR Green I Master Mix (Roche #04-887-352-001) on a LightCycler® Real-Time PCR System (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... A total of 50 ng DNase-treated RNA was used as template for reverse transcription using either RT Transcriptor from Roche and random hexamers or Moloney Murine Leukemia Virus-RT (MMULV-RT ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was eluted from the beads by incubation for 1h at RT (while rotating) with 150-250 µl wash buffer supplemented with phosphatase inhibitors (PhosSTOP, Roche) and 0.5 mg/ml 3xFLAG peptide (APExBIO) ...
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bioRxiv - Microbiology 2019Quote: ... After washing with 1xPBS containing 0.1% v/v Tween-20, PIP–strips were incubated (1h, RT, agitated) with a monoclonal anti-HA antibody (clone 3F10, monoclonal antibody from Roche) at a dilution of 1:500 in blocking buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... The RNA concentration was measured by a spectrophotometer (NanDrop1000; Thermo) followed by a reverse transcription process using PrimeScript RT reagent kit (Roche). Real time PCR analysis was performed using SYBR green mix (Roche ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR experiments were done with SYBR Green I in a Light Cycler 96® (Roche Applied Science, Mannheim, Germany). For each primer pair (Table 4 ...
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bioRxiv - Neuroscience 2021Quote: ... diluted in 1x maleic acid for 1h at RT and riboprobes were detected by incubating in anti-digoxigenin-AP antibody (1:2000 in 1% sheep serum, Roche) overnight at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... Real-time RT-qPCR reactions were run in a 384-well LightCycler®480 II System (Roche Diagnostics, Mannheim, Germany) using the GoTaq qPCR Mastermix (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNAse was inactivated with 1 µl of Stop Solution at 65°C for 10 min and RT was performed using Transcriptor First Strand cDNA Synthesis Kit (Ref. 04 897 030 001, Roche) in a final volume of 20 µl ...
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bioRxiv - Cell Biology 2021Quote: ... for 1h at RT and incubated with a primary antibody diluted in blocking solution (sheep anti-digoxigenin, 1/200, Roche) overnight at +4°C in a dark humidified chamber ...
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bioRxiv - Microbiology 2020Quote: ... Library concentrations for pooling of barcoded samples was assessed by RT-qPCR with a KAPA Library Quantification kit (KAPA Biosystems) as recommended for high sensitivity ...
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bioRxiv - Cell Biology 2020Quote: ... TNbuffer 10 min at RT and TNT buffer 15’ at RT) and by the incubation of cryo-section using anti-digoxigenin-ap fab fragments (1/100, #11093274910, Roche) dissolved in TNbuffer for 2 hours at RT ...
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bioRxiv - Developmental Biology 2022Quote: ... The mRNA was used in an RT reaction that was specifically selected for the barcoded mRNA (Transcriptor High Fidelity Roche). The RT product was PCR amplified and size selected using Agencourt AMPure beads (Beckman Coulter) ...
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bioRxiv - Genomics 2019Quote: Validation of the RNA-Seq experiment was completed with the Universal Probe Library (UPL) RT-qPCR system (Roche Diagnostics ©). RNA samples analyzed were those used for RNAseq analyses (aphid collections from location 1 ...
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bioRxiv - Microbiology 2019Quote: ... RT-qPCR analysis of cytokine related porcine gene expression was performed using the KAPA SYBR-FAST qPCR Kit (KAPA Biosystems), using the primers shown in Table 1 ...
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bioRxiv - Developmental Biology 2020Quote: ... They were then blocked in PBS with 3% BSA for 3h at RT and incubated overnight at 4°C with a peroxidase-labeled anti-DIG antibody (Roche) diluted 1:1500 in PBS with 3% BSA ...
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bioRxiv - Biochemistry 2020Quote: RT activity was measured using an ELISA-based colorimetric reverse transcriptase activity assay (Catalog No. 11468120910, Roche Diagnostics, Indianapolis, IN). The HEPES integration buffer was used for the reaction ...
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bioRxiv - Cell Biology 2021Quote: ... A volume corresponding to 25-50 ng of cDNA was used for each RT-qPCR reaction using Roche LightCycler 480 SYBR Green I Master (04707516001, Roche) sequence detection system ...
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bioRxiv - Immunology 2020Quote: ... RT-qPCR was performed using the LightCycler 480 Probe Master Kit and the LightCycler 480 II system (Roche Applied Science) as described previously92 ...
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bioRxiv - Microbiology 2021Quote: ... The samples were subsequently diluted by adding 150 μL of water and 4 μL was used for RT-qPCR performed on a LightCycler96 platform (Roche) using the iTaq Universal Probes One-Step RT-qPCR kit (BioRad ...
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bioRxiv - Microbiology 2021Quote: ... Library concentration for pooling of barcoded samples was assessed by RT-qPCR with a KAPA Library Quantification kit (KAPA Biosystems) as recommended for high sensitivity ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was then washed and incubated 1 hs at RT with goat anti-mouse secondary antibody conjugated to HRP (Roche) at 1/20000 dilution in PBST - 5% milk.
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bioRxiv - Physiology 2023Quote: ... Each RT-qPCR assay was deposited in triplicates on a FrameStar® 384-well skirted qPCR plate (Roche Diagnostics, Switzerland). The qPCR program was initiated at 95°C for 10 minutes to denature the cDNA and activate the TAQ polymerase enzyme in a thermocycler (Lightcycler® 480 II Roche thermocycler ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were pelleted by centrifugation (750 x g, 2 min, RT) and washed in 4 ml vPBS containing EDTA-free protease inhibitor (Roche). The washed cells were again pelleted by centrifugation (750 x g ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR amplification was performed using KAPA HiFi (Roche KK2101) for all amplifications with plasmid or phage templates ...
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bioRxiv - Genetics 2021Quote: ... PCR with Kapa HiFi HotStart ReadyMix (Roche, Basel, Switzerland) was performed on the cDNA using M28 virus-specific primer pairs ...
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bioRxiv - Developmental Biology 2021Quote: ... using SYBRgreen PCR master mix (Kapa Biosystems, London, UK). 21.5 ng of cDNA were loaded ...
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bioRxiv - Immunology 2019Quote: ... on the Roche real-time PCR system (Roche 480). Values were normalized to Rps17 gene for each sample ...
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bioRxiv - Molecular Biology 2021Quote: Using the LightCycler 96 Real-Time PCR System (Roche), the following cycling conditions were used ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative PCR was performed using a LightCycler (Roche Diagnostics). UBQ14 (for VISUAL experiments ...
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bioRxiv - Neuroscience 2021Quote: ... on a LightCycler® Real-Time PCR System (Roche). The final volume for each reaction was 20 μl with 500 nM of corresponding gene specific primers (Snhg11 forward primer ...
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bioRxiv - Molecular Biology 2020Quote: ... using either 480 SYBR Green LightCycler PCR mix (Roche), SsoAdvanced Universal SYBR mix (Bio-Rad ...
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bioRxiv - Cancer Biology 2019Quote: ... 1x FastStart PCR Buffer including 2 μM MgCl2 (Roche), 0.05 mm dNTPs (Roche) ...
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bioRxiv - Immunology 2020Quote: ... Using KAPA HiFi HotStart ReadyMix PCR Kit (Kapa Biosystems) the two 5’UTRs were amplified from cDNA.
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bioRxiv - Developmental Biology 2019Quote: ... using a LightCycler 480 Real-Time PCR System (Roche). The relative expression level of each target gene was determined using glyceraldehyde-3 phosphate dehydrogenase (GAPDH ...
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bioRxiv - Microbiology 2019Quote: ... The LightCycler 480 Real-Time PCR System (Roche Diagnostics) was used for thermal cycling ...
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bioRxiv - Genetics 2019Quote: ... PCR reactions included 5µL HiFi HotStart ReadyMix (KAPA Biosystems), 3.2µL primer mix (1.25µM) ...
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bioRxiv - Genomics 2020Quote: ... Seven cycles of PCR using HiFi polymerase (Kapa Biosystems) was used to enrich the bisulfite converted DNA and introduce fault tolerant hexamer barcode sequences ...
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bioRxiv - Cancer Biology 2021Quote: ... real time quantitative PCR using sybr-green (Kapa Biosystems) and the StepOnePlus™ Real-Time PCR Systems (Life Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative PCR was performed using FastStart SYBR Green (Roche) on a Lightcycler 480 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and Expand High Fidelity PCR System (Roche Life Science) according to manufacturer’s protocols ...
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bioRxiv - Genetics 2021Quote: ... qRT-PCR was performed with SYBR Green reagent (Roche) using Lightcycler 480 (Roche ...