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Citations for Roche :
1351 - 1400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... using X-tremeGENE HP DNA transfection reagent (6366546001, Sigma-Aldrich/Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantification was performed on a LightCycler 480 (Roche). Target of interest values were expressed relative to the corresponding PPIA values using the ΔΔCt method ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted from culture plates using Tri-Reagent (Roche) and isolated using Direct-Zol RNA miniprep kits (ZYMO RESEARCH) ...
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bioRxiv - Cancer Biology 2024Quote: ... and protease inhibitors (Complete, Roche).
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bioRxiv - Cancer Biology 2024Quote: ... Cell extracts were obtained by washing 3 × in PBS and either lysing for RNA (TriPure reagent, Roche) or protein extraction (50 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed with PBS and lysed in radioimmunoprecipitation assay (RIPA) buffer supplemented with protease (SERVA) and phosphatase inhibitors (Roche). The protein concentration was determined using a Bicinchoninic Acid Protein (BCA ...
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bioRxiv - Cancer Biology 2024Quote: ... resuspended in lysis buffer (20 mM HEPES, 500 mM NaCl and 10 mM imidazole, pH 7.5) with cOmplete protease inhibitor Cocktail (Roche), and lysed by a sonicator (Q125 ...
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bioRxiv - Cancer Biology 2024Quote: ... If stained with phalloidin (1/500, Scientific Laboratory Supplies P1951) and DAPI (1/1000 in PBS from 1 mg/ml stocks, Roche 10236276001), or just DAPI ...
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bioRxiv - Cancer Biology 2024Quote: Total proteins were extracted from cultured NB cells using RIPA buffer supplemented with protease and phosphatase inhibitor cocktail (Roche). The cell lysates were pre-cleared with protein A or protein G beads (Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2024Quote: Tissue or cells were washed with cold PBS and lysed in RIPA buffer containing a protease and phosphatase inhibitor cocktail (Roche Diagnostics, Indianapolis, IN, USA). Proteins were analyzed by SDS-PAGE ...
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bioRxiv - Cancer Biology 2024Quote: ... and 0.1% SDS) supplemented with CompleteTM protease inhibitor cocktail (Roche, Switzerland). The extracted protein lysates were denatured with 4X SDS sample buffer (200mM Tris-HCl pH 6.8 ...
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bioRxiv - Cell Biology 2024Quote: ... and 0.075% NP-40) supplemented with 1 Complete EDTA-free protease inhibitor cocktail (Roche), 20 mM beta-glycerophosphate ...
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bioRxiv - Cell Biology 2024Quote: ... 6 uL X-tremegene-9 transfection reagent (Roche 06365787001), 0.3 μg pCMV-VSV-G (Addgene Plasmid #8454) ...
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bioRxiv - Cancer Biology 2024Quote: ... Proliferation rate in vitro was assessed using an xCELLigence Real-Time Cell Analyzer (RTCA) system (Roche Diagnostics)49 ...
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bioRxiv - Cancer Biology 2024Quote: ... Protease Inhibitor Cocktail (Roche Diagnostics), 1 mM β-glycerophosphate ...
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bioRxiv - Cancer Biology 2024Quote: ... The pooled library was quantified using KAPA library quantification kit (Roche KK4824) and equimolar libraries were pooled and subjected to 75-bp paired-end sequencing on a NextSeq 550 machine (Illumina) ...
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bioRxiv - Cancer Biology 2024Quote: ... The size and concentration of each sample’s library were determined using Agilent 2200 Tapestation and KAPA library quantification kit (Roche, #KK4824) respectively ...
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bioRxiv - Cancer Biology 2024Quote: ... an enzymatic cocktail of collagenase A (1mg/mL; Roche) and Dispase II (2mg/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% Triton X-100) with protease and phosphatase inhibitors (Roche). Protein concentrations were measured using the DC Protein Assay Kit (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: ... and specific primers on the LightCycler 96 (Roche). Relative target gene expression was determined by comparing average threshold cycles (CT ...
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bioRxiv - Cancer Biology 2024Quote: ... and phosphatase inhibitors (#4906837001, Roche). 15-25μg of proteins were run on NuPage 4-12% gradient Bis-Tris gels in NuPAGE™ MES SDS Running Buffer (20X ...
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bioRxiv - Cancer Biology 2024Quote: ... 10% glycerol + Complete protease inhibitor cocktail (Roche), phosphatase inhibitor (Sigma)) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were transfected with 1 µg of shRNA/cDNA and 1 µg of viral packaging plasmids (250 ng pMD2.G and 750 ng psPAX2) using 6 µl of Xtreme Gene 9 transfection reagent (Roche). After 24 hours of transfection ...
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bioRxiv - Developmental Biology 2024Quote: ... or incubated in 1X RBC lysis solution (Roche #11814389001) for 10 minutes at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: RNA-seq libraries were constructed by the ICM Platform (PARIS) from 100 ng of total RNA using the KAPA mRNA Hyperprep kit (Roche) that allows to obtain stranded polyA libraries ...
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bioRxiv - Developmental Biology 2024Quote: ... T7 DNA ligase (Roche) 25ng of backbone vector ...
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bioRxiv - Developmental Biology 2024Quote: ... Nuclei were washed once in 1 mL Wash350 (20 mM HEPES pH 7.5, 350 mM NaCl, 10 mM NaButyrate, 0.025% Digitonin, protease inhibitor cocktail (Roche), 0.5 mM Sper-midine ...
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bioRxiv - Developmental Biology 2024Quote: ... Phosphorylated and annealed oligos were diluted 1:10 and a Golden Gate reaction was setup with 1x rapid ligase (Roche), 10 units of BbsI enzyme ...
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bioRxiv - Developmental Biology 2024Quote: ... purified through a column (Roche), and final concentrations were measured using a Nanodrop 3300 spectrometer (Thermoscientific) ...
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bioRxiv - Developmental Biology 2024Quote: Primary tissue samples from reduction mammoplasties of healthy women were dissociated through a 3 mg/mL collagenase (Roche Life Science) solution and sorted via density gradient ...
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bioRxiv - Developmental Biology 2024Quote: ... The pIEx-4-BmU6-Cad96ca-gRNA-Cas9-GFP-P2A-Puro or pIEx-4-BmU6-Fgfr1-gRNA-Cas9-GFP-P2A-Puro recombinant vectors were transfected into HaEpi cells with transfection reagent (Roche, Basel, Switzerland). After 48 h of vector transfection (cells can be observed to express green fluorescent protein) ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were incubated in a blocking solution (0.5% Roche Western Blocking Reagent and 5% inactivated horse serum in TNTx ...
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bioRxiv - Developmental Biology 2024Quote: ... the tail of each embryo was collected for genomic DNA extraction using the KAPA Mouse Genotyping Kit HotStart (Kapa Biosystems, KK7352). Subsequently ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR products with flanking T7 promoters were used as template for the synthesis of anti-sense riboprobes using a T7 RNA polymerase (Roche) (Mercurio et al ...
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bioRxiv - Developmental Biology 2024Quote: ... The remaining lysate was rotated overnight with 50 µl of Protein A-agarose beads (Roche) and 2 µg of mouse anti-HA (monoclonal ...
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bioRxiv - Developmental Biology 2024Quote: ... EDTA-free Protease Inhibitor Cocktail (Roche, 11836170001). The resulting cell lysates underwent brief sonication and centrifugation ...
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bioRxiv - Developmental Biology 2024Quote: ... Incubation with anti-digoxigenin-AP Fab antibody (Roche), 1:2000 in PBST ...
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bioRxiv - Developmental Biology 2024Quote: ... and a digoxigenin labeling mix (Roche, catalog#: 11277073910). The synthesized riboprobes were treated with DNase I digestion to remove the DNA template ...
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bioRxiv - Developmental Biology 2024Quote: ... Animals were incubated with an anti-DIG-POD antibody (1:1,500; Roche) or anti-DNP-HRP (1:10,000 ...
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bioRxiv - Developmental Biology 2024Quote: ... EDTA free protease inhibitor cocktail (Roche)) at left on ice for 10 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... washed once with PBS and resuspended and cryopreserved in wash buffer 150 (20 mM HEPES pH 7.5, 150 mM NaCl, protease inhibitor cocktail (Roche), 0.5 mM Spermidine ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 phosphatase tablet (PhosSTOP – Roche), 20 μM MG132 ...
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bioRxiv - Cancer Biology 2024Quote: ... Isolated hepatocytes were seeded at a density of 4-500,000 and 1,000,000 cells in rat tail collagen I (5 μg/cm2, Roche) pre-coated 6-well plates and T25 flasks ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA plasmids were transfected using X-tremeGENE HP (Roche Diagnostics) whereas siRNA was delivered using Lipofectamine™ RNAiMAX (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Staining reaction was performed using BMP-Purple (Roche). Zebrafish larvae xenografts were photographed using a Zeiss SteREO Discovery.V8 coupled to a Zeiss AxioCam Icc 3 Camera.
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bioRxiv - Cell Biology 2024Quote: ... rat monoclonal anti-HA (Roche, Cat. No. 11 867 423 001; 1:2,000); rabbit polyclonal anti-Slp1 (Kim et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... Cell lines were transfected with siRNAs using xTremeGene siRNA transfection reagent (1ul/well, Roche, #4476093001; standard protocol). After addition of siRNA transfection mix ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA libraries were prepared using the KAPA mRNA HyperPrep Kit (Roche) and subsequently sequenced on a NextSeq 500 sequencer (paired-end 43 base pairs ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... The FastStart Universal SYBR Green Master (Roche) was used for all reactions ...
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bioRxiv - Cell Biology 2024Quote: ... and 1x cOmplete Mini protease inhibitor cocktail (Roche, Mannheim, Germany)) ...